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1.
Cell Mol Neurobiol ; 41(5): 949-960, 2021 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32107752

RESUMEN

OBJECTIVE: Pharmacological evaluation of the mu-opioid receptor (MOR) agonist properties of NKTR-181 in rodent models. METHODS: Graded noxious stimulus intensities were used in rats to establish the antinociceptive potency and efficacy of NKTR-181 relative to morphine, fentanyl, and oxycodone. Characteristics of MOR agonist actions, as measured by antinociceptive tolerance and cross-tolerance, as well as opioid-induced hyperalgesia (OIH) and naloxone-precipitated withdrawal in NKTR-181- and morphine-dependent in mice, were compared. RESULTS: NKTR-181 showed dose- and time-related antinociception with similar maximal effects to morphine in the rat and mouse hot-water tail-flick test. No sex or species differences were observed in NKTR-181 or morphine antinociception. Rats treated with NKTR-181 and morphine exhibited decreases in both potency and maximal efficacy as nociceptive stimulus intensity was increased from a water temperature of 50 °C to 54 °C. Evaluation of antinociception at a high stimulus intensity revealed that oxycodone and fentanyl exhibited greater efficacy than either NKTR-181 or morphine. The relative potency difference between NKTR-181 and morphine across all tail-flick studies was determined to be 7.6-fold (90% confidence interval, 2.6, 21.5). The peak antinociceptive effect of NKTR-181 was delayed compared to that of the other opioids and cumulative drug effects were not observed. Repeated treatment with escalating, approximately equi-analgesic doses of NKTR-181 or morphine, produced antinociceptive tolerance and cross-tolerance. Under these pharmacological conditions, OIH and naloxone-precipitated physical dependence were similar for NKTR-181 and morphine. CONCLUSIONS: NKTR-181 had a slower onset, but similar efficacy, to morphine in the models studied supporting reduced abuse potential while maintaining analgesic effect in comparison with current opioids.


Asunto(s)
Analgésicos Opioides/farmacología , Morfinanos/farmacología , Morfina/farmacología , Dimensión del Dolor/efectos de los fármacos , Receptores Opioides mu/agonistas , Animales , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos/métodos , Femenino , Masculino , Ratones , Dimensión del Dolor/métodos , Ratas , Ratas Sprague-Dawley , Receptores Opioides mu/fisiología , Roedores
2.
Curr Biol ; 29(24): 4291-4299.e4, 2019 12 16.
Artículo en Inglés | MEDLINE | ID: mdl-31786059

RESUMEN

Mammals maintain a nearly constant core body temperature (Tb) by balancing heat production and heat dissipation. This comes at a high metabolic cost that is sustainable if adequate calorie intake is maintained. When nutrients are scarce or experimentally reduced such as during calorie restriction (CR), endotherms can reduce energy expenditure by lowering Tb [1-6]. This adaptive response conserves energy, limiting the loss of body weight due to low calorie intake [7-10]. Here we show that this response is regulated by the kappa opioid receptor (KOR). CR is associated with increased hypothalamic levels of the endogenous opioid Leu-enkephalin, which is derived from the KOR agonist precursor dynorphin [11]. Pharmacological inhibition of KOR, but not of the delta or the mu opioid receptor subtypes, fully blocked CR-induced hypothermia and increased weight loss during CR independent of calorie intake. Similar results were seen with DIO mice subjected to CR. In contrast, inhibiting KOR did not change Tb in animals fed ad libitum (AL). Chemogenetic inhibition of KOR neurons in the hypothalamic preoptic area reduced the CR-induced hypothermia, whereas chemogenetic activation of prodynorphin-expressing neurons in the arcuate or the parabrachial nucleus lowered Tb. These data indicate that KOR signaling is a pivotal regulator of energy homeostasis and can affect body weight during dieting by modulating Tb and energy expenditure.


Asunto(s)
Regulación de la Temperatura Corporal/genética , Regulación de la Temperatura Corporal/fisiología , Receptores Opioides kappa/metabolismo , Analgésicos Opioides/metabolismo , Animales , Peso Corporal/fisiología , Encéfalo/metabolismo , Restricción Calórica/métodos , Ingestión de Energía/fisiología , Metabolismo Energético/fisiología , Femenino , Hipotálamo/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Neuronas/metabolismo , Receptores Opioides kappa/genética , Receptores Opioides mu/metabolismo , Receptores Opioides mu/fisiología , Pérdida de Peso/fisiología
3.
Int J Clin Exp Hypn ; 66(1): 106-118, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29319460

RESUMEN

The possible cooperation between hypnotizability-related and placebo mechanisms in pain modulation has not been consistently assessed. Here, we investigate possible genetic bases for such cooperation. The OPRM1 gene, which encodes the µ1 opioid receptor-the primary site of action for endogenous and exogenous opioids-is polymorphic in the general population for the missense mutation Asn40Asp (A118G, rs1799971). The minor allele 118G results in decreased levels of OPRM1 mRNA and protein. As a consequence, G carriers are less responsive to opioids. The aim of the study was to investigate whether hypnotizability is associated with the presence of the OPRM1 polymorphism. Forty-three high and 60 low hypnotizable individuals, as well as 162 controls, were genotyped for the A118G polymorphism of OPRM1. The frequency of the G allele was significantly higher in highs compared to both lows and controls. Findings suggest that an inefficient opioid system may be a distinctive characteristic of highs and that hypnotic assessment may predict lower responsiveness to opioids.


Asunto(s)
Hipnosis , Receptores Opioides mu/genética , Estudios de Casos y Controles , Frecuencia de los Genes/genética , Humanos , Polimorfismo de Nucleótido Simple/genética , Polimorfismo de Nucleótido Simple/fisiología , Receptores Opioides mu/fisiología
4.
J Neuroinflammation ; 14(1): 83, 2017 04 14.
Artículo en Inglés | MEDLINE | ID: mdl-28407740

RESUMEN

BACKGROUND: Opioid receptors are known to control neurotransmission of various peptidergic neurons, but their potential role in regulation of microglia and neuronal cell communications is unknown. We investigated the role of mu-opioid receptors (MOR) and delta-opioid receptors (DOR) on microglia in the regulation of apoptosis in proopiomelanocortin (POMC) neurons induced by neonatal ethanol in the hypothalamus. METHODS: Neonatal rat pups were fed a milk formula containing ethanol or control diets between postnatal days 2-6. Some of the alcohol-fed rats additionally received pretreatment of a microglia activation blocker minocycline. Two hours after the last feeding, some of the pups were sacrificed and processed for histochemical detection of microglial cell functions or confocal microscopy for detection of cellular physical interaction or used for gene and protein expression analysis. The rest of the pups were dissected for microglia separation by differential gradient centrifugation and characterization by measuring production of various activation markers and cytokines. In addition, primary cultures of microglial cells were prepared using hypothalamic tissues of neonatal rats and used for determination of cytokine production/secretion and apoptotic activity of neurons. RESULTS: In the hypothalamus, neonatal alcohol feeding elevated cytokine receptor levels, increased the number of microglial cells with amoeboid-type circularity, enhanced POMC and microglial cell physical interaction, and decreased POMC cell numbers. Minocycline reversed these cellular effects of alcohol. Alcohol feeding also increased levels of microglia MOR protein and pro-inflammatory signaling molecules in the hypothalamus, and MOR receptor antagonist naltrexone prevented these effects of alcohol. In primary cultures of hypothalamic microglia, both MOR agonist [D-Ala 2, N-MePhe 4, Gly-ol]-enkephalin (DAMGO) and ethanol increased microglial cellular levels and secretion of pro-inflammatory cell signaling proteins. However, a DOR agonist [D-Pen2,5]enkephalin (DPDPE) increased microglial secretion of anti-inflammatory cytokines and suppressed ethanol's ability to increase microglial production of inflammatory signaling proteins and secretion of pro-inflammatory cytokines. In addition, MOR-activated inflammation promoted while DOR-suppressed inflammation inhibited the apoptotic effect of ethanol on POMC neurons. CONCLUSIONS: These results suggest that ethanol's neurotoxic action on POMC neurons results from MOR-activated neuroinflammatory signaling. Additionally, these results identify a protective effect of a DOR agonist against the pro-inflammatory and neurotoxic action of ethanol.


Asunto(s)
Etanol/toxicidad , Microglía/metabolismo , Neuronas/metabolismo , Proopiomelanocortina/metabolismo , Receptores Opioides delta/fisiología , Receptores Opioides mu/fisiología , Animales , Animales Recién Nacidos , Apoptosis/efectos de los fármacos , Apoptosis/fisiología , Células Cultivadas , Encefalina Ala(2)-MeFe(4)-Gli(5)/farmacología , Femenino , Hipotálamo/efectos de los fármacos , Hipotálamo/metabolismo , Hipotálamo/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Microglía/efectos de los fármacos , Microglía/patología , Neuronas/efectos de los fármacos , Neuronas/patología , Ratas , Ratas Sprague-Dawley , Receptores Opioides delta/agonistas , Receptores Opioides mu/agonistas
5.
Alcohol Clin Exp Res ; 41(3): 637-643, 2017 03.
Artículo en Inglés | MEDLINE | ID: mdl-28166603

RESUMEN

BACKGROUND: Hyperalgesia or increased sensitivity to pain is often found in alcoholics during alcohol withdrawal and may contribute to relapse drinking. Alternative therapies such as acupuncture and electroacupuncture (EA), through mechanisms involving opioid receptors, may reduce pain and substance dependence and withdrawal syndromes. The lateral habenula (LHb), an epithalamic structure rich in mu opioid receptors (MORs), is a critical target for both drugs of abuse and pain. We previously observed hyperalgesia in rats withdrawn from chronic ethanol (EtOH) drinking and found that EA at the acupoint Zusanli (ST36) reduced EtOH intake. This raised question of whether EA can alleviate hyperalgesia during alcohol withdrawal and, if so, whether the mechanism involves MORs in the LHb. METHODS: We trained male rats to drink EtOH using the intermittent access 20% EtOH 2-bottle free-choice drinking paradigm for 8 weeks, after which the alcohol supply was discontinued. We measured pain sensitivity using radiant heat (a light beam directed at the hind paw of rats) and compared the paw withdrawal latencies (PWLs) with and without EA at ST36. RESULTS: The PWLs were significantly shorter in rats at 24, 48, and 72 hours and 7 days after the discontinuation of EtOH when compared to EtOH-naïve rats. After a single administration of 2-Hz EA for 20 minutes at ST36, the PWLs at 24 hours after the withdrawal of EtOH were significantly greater than those of the sham group (2-Hz EA at the tail). Furthermore, the effect of EA on PWLs was significantly attenuated by bilateral intrahabenula infusion of the MOR antagonist naltrexone. CONCLUSIONS: These results suggest that EA can alleviate hyperalgesia during EtOH withdrawal through a mechanism involving MORs in the habenula. Based on this, EA could be of potential value as a therapy for hyperalgesia in alcohol dependence.


Asunto(s)
Alcoholismo/terapia , Electroacupuntura/métodos , Habénula/efectos de los fármacos , Hiperalgesia/prevención & control , Receptores Opioides mu/antagonistas & inhibidores , Síndrome de Abstinencia a Sustancias/terapia , Consumo de Bebidas Alcohólicas/efectos adversos , Consumo de Bebidas Alcohólicas/terapia , Alcoholismo/complicaciones , Animales , Habénula/fisiología , Hiperalgesia/etiología , Masculino , Microinyecciones , Antagonistas de Narcóticos/administración & dosificación , Ratas , Ratas Long-Evans , Receptores Opioides mu/fisiología , Síndrome de Abstinencia a Sustancias/etiología
6.
Eur J Pharmacol ; 736: 63-9, 2014 Aug 05.
Artículo en Inglés | MEDLINE | ID: mdl-24815321

RESUMEN

The opioid and nociceptin systems play a crucial role in the maintenance of homeostasis in the gastrointestinal (GI) tract. The aim of this study was to characterize the effect of BU08070, a novel mixed MOP/NOP agonist, on mouse intestinal contractility in vitro and GI motility in vivo in physiological conditions and in animal models mimicking symptoms of irritable bowel syndrome (IBS), including diarrhea and abdominal pain. The effect of BU08070 on muscle contractility in vitro was characterized in the ileum and colon. To assess the effect of BU08070 in vivo, the following parameters were assessed: whole GI transit, gastric emptying, geometric center, colonic bead expulsion, fecal pellet output and time to castor oil-induced diarrhea. The antinociceptive activity of BU08070 was characterized in the mustard oil (MO)-induced abdominal pain model and the writhing test, alone and in the presence of MOP and NOP antagonists. in vitro, BU08070 (10(-10)-10(-6) M) inhibited colonic and ileal smooth muscle contractions in a concentration-dependent manner. in vivo, BU08070 prolonged the whole GI transit and inhibited colonic bead expulsion. The antitransit and antidiarrheal effects of BU08070 were observed already at the dose of 0.1 mg/kg (i.p.). BU08070 reversed hypermotility and reduced pain in mouse models mimicking IBS-D symptoms. Our results suggest that BU08070 has a potential of becoming an efficient drug in IBS-D therapy. Here we also validate mixed NOP/MOP receptor targeting as possible future treatment of functional GI diseases.


Asunto(s)
Dolor Abdominal/tratamiento farmacológico , Analgésicos Opioides/uso terapéutico , Buprenorfina/análogos & derivados , Diarrea/tratamiento farmacológico , Síndrome del Colon Irritable/tratamiento farmacológico , Dolor Abdominal/inducido químicamente , Analgésicos Opioides/farmacología , Animales , Buprenorfina/farmacología , Buprenorfina/uso terapéutico , Aceite de Ricino , Colon/efectos de los fármacos , Colon/fisiología , Diarrea/inducido químicamente , Modelos Animales de Enfermedad , Vaciamiento Gástrico/efectos de los fármacos , Motilidad Gastrointestinal/efectos de los fármacos , Íleon/efectos de los fármacos , Íleon/fisiología , Técnicas In Vitro , Masculino , Ratones Endogámicos BALB C , Contracción Muscular/efectos de los fármacos , Músculo Liso/efectos de los fármacos , Músculo Liso/fisiología , Planta de la Mostaza , Aceites de Plantas , Receptores Opioides/agonistas , Receptores Opioides/fisiología , Receptores Opioides mu/agonistas , Receptores Opioides mu/fisiología , Receptor de Nociceptina
7.
Brain Res ; 1448: 63-70, 2012 Apr 11.
Artículo en Inglés | MEDLINE | ID: mdl-22381895

RESUMEN

We have shown previously that intracerebroventricular (icv) injection of naloxone (a non-selective opioid receptor antagonist) or naloxonazine (a selective µ1-opioid receptor antagonist) at the maintenance phase of hibernation arouses Syrian hamsters from hibernation. This study was designed to clarify the role of ß-endorphin (an endogenous µ-opioid receptor ligand) on regulation of body temperature (T(b)) during the maintenance phase of hibernation. The number of c-Fos-positive cells and ß-endorphin-like immunoreactivity increased in the arcuate nucleus (ARC) after hibernation onset. In contrast, endomorphin-1 (an endogenous µ-opioid receptor ligand)-like immunoreactivity observed on the anterior hypothalamus decreased after hibernation onset. In addition, hibernation was interrupted by icv injection of anti-ß-endorphin antiserum at the maintenance phase of hibernation. The mRNA expression level of proopiomelanocortin (a precursor of ß-endorphin) on ARC did not change throughout the hibernation phase. However, the mRNA expression level of prohormone convertase-1 increased after hibernation onset. [D-Ala2,N-MePhe4,Gly-ol5] enkephalin (DAMGO, a selective µ-opioid receptor agonist) microinjection into the dorsomedial hypothalamus (DMH) elicited the most marked T(b) decrease than other sites such as the preoptic area (PO), anterior hypothalamus (AH), lateral hypothalamus (LH), ventromedial hypothalamus and posterior hypothalamus (PH). However, microinjected DAMGO into the medial septum indicated negligible changes in T(b). These results suggest that ß-endorphin which synthesizes in ARC neurons regulates T(b) during the maintenance phase of hibernation by activating µ-opioid receptors in PO, AH, VMH, DMH and PH.


Asunto(s)
Regulación de la Temperatura Corporal/fisiología , Sistema Nervioso Central/fisiología , Hibernación/fisiología , betaendorfina/fisiología , Analgésicos Opioides/farmacología , Animales , Química Encefálica/fisiología , Recuento de Células , Cricetinae , Encefalina Ala(2)-MeFe(4)-Gli(5)/farmacología , Hipotálamo/fisiología , Hipotermia/fisiopatología , Inmunohistoquímica , Inyecciones Intraventriculares , Mesocricetus , Oligopéptidos/metabolismo , Reacción en Cadena de la Polimerasa , Proproteína Convertasa 1/biosíntesis , Proteínas Proto-Oncogénicas c-fos/metabolismo , Receptores Opioides mu/fisiología
8.
Pharmacol Biochem Behav ; 101(4): 625-31, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22446386

RESUMEN

Recent work has examined the relationship between stress and relapse to alcohol use in clinical populations. Few prospective studies, however, have examined stress as a precipitant of alcohol problems. The present study is a longitudinal examination of the role of stress reactivity and alcohol craving in the etiology of alcohol problems in a sample of 41 (mean age=20.8), heavy-drinking, young adults. Participants completed a guided imagery exposure to stressful life events, followed by exposure to a neutral imagery control. Following the exposure, participants completed an alcohol cue exposure paradigm. Measures of negative mood (Profile of Mood States (POMS) depression/dejection scale), tension (POMS tension/anxiety scale) and alcohol craving (measured by the Alcohol Urge Questionnaire (AUQ)) were used as indicators of reactivity to stress and to alcohol cues. Polymorphisms of the corticotropin-releasing hormone binding protein (CRH-BP) gene and of the µ-opioid receptor (OPRM1) gene were examined as moderators of this relationship. Results revealed that stress-induced negative mood predicted negative consequences of drinking (scores on the Drinker's Inventory of Consequences (DrInC-2R)), whereas stress and cue-induced alcohol craving did not predict alcohol use or problems. Additionally, the CRH-BP genotype was found to moderate the relationship between stress-induced negative affect and the negative consequences of drinking. The current study supports and extends laboratory research describing phenotypes of stress-induced alcohol craving.


Asunto(s)
Consumo de Bebidas Alcohólicas/psicología , Alcoholismo/psicología , Estrés Psicológico , Adolescente , Afecto , Consumo de Bebidas Alcohólicas/genética , Consumo de Bebidas Alcohólicas/fisiopatología , Alcoholismo/genética , Alcoholismo/fisiopatología , Proteínas Portadoras/genética , Proteínas Portadoras/fisiología , Femenino , Humanos , Masculino , Polimorfismo Genético , Estudios Prospectivos , Receptores Opioides mu/genética , Receptores Opioides mu/fisiología , Adulto Joven
9.
Horm Behav ; 60(5): 540-8, 2011 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-21872598

RESUMEN

Sexual receptivity, lordosis, can be induced by sequential estradiol and progesterone or extended exposure to high levels of estradiol in the female rat. In both cases estradiol initially inhibits lordosis through activation of ß-endorphin (ß-END) neurons of the arcuate nucleus of the hypothalamus (ARH) that activate µ-opioid receptors (MOP) in the medial preoptic nucleus (MPN). Subsequent progesterone or extended estradiol exposure deactivates MPN MOP to facilitate lordosis. Opioid receptor-like receptor-1 (ORL-1) is expressed in ARH and ventromedial hypothalamus (VMH). Infusions of its endogenous ligand, orphanin FQ (OFQ/N, aka nociceptin), into VMH-ARH region facilitate lordosis. Whether OFQ/N acts in ARH and/or VMH and whether OFQ/N is necessary for steroid facilitation of lordosis are unclear. In Exp I, OFQ/N infusions in VMH and ARH that facilitated lordosis also deactivated MPN MOP indicating that OFQ/N facilitation of lordosis requires deactivation of ascending ARH-MPN projections by directly inhibiting ARH ß-END neurons and/or through inhibition of excitatory VMH-ARH pathways to proopiomelanocortin neurons. It is unclear whether OFQ/N activates the VMH output motor pathways directly or via the deactivation of MPN MOP. In Exp II we tested whether ORL-1 activation is necessary for estradiol-only or estradiol+progesterone lordosis facilitation. Blocking ORL-1 with UFP-101 inhibited estradiol-only lordosis and MPN MOP deactivation but had no effect on estradiol+progesterone facilitation of lordosis and MOP deactivation. In conclusion, steroid facilitation of lordosis inhibits ARH ß-END neurons to deactivate MPN MOP, but estradiol-only and estradiol+progesterone treatments appear to use different neurotransmitter systems to inhibit ARH-MPN signaling.


Asunto(s)
Hipotálamo/efectos de los fármacos , Péptidos Opioides/fisiología , Postura/fisiología , Conducta Sexual Animal/fisiología , Animales , Estradiol/administración & dosificación , Femenino , Hipotálamo/fisiología , Masculino , Péptidos Opioides/administración & dosificación , Péptidos Opioides/antagonistas & inhibidores , Péptidos Opioides/farmacología , Área Preóptica , Progesterona/administración & dosificación , Ratas , Ratas Long-Evans , Receptores Opioides mu/fisiología , Conducta Sexual Animal/efectos de los fármacos , Nociceptina
10.
Brain Res ; 1395: 38-45, 2011 Jun 13.
Artículo en Inglés | MEDLINE | ID: mdl-21565329

RESUMEN

It has been reported that intracerebroventricular injection of a µ receptor antagonist blocked 2 but not 100Hz electroacupuncture (EA)-produced analgesia in an uninjured animal model. Because persistent pain changes neural response to external stimulation, we hypothesized that the mechanisms of EA anti-hyperalgesia may be different in persistent pain than in health. Hyperalgesia, decreased paw withdrawal latency (PWL) to a noxious thermal stimulus, was induced by subcutaneously injecting complete Freund's adjuvant (CFA) into the hind paws of rats. Selective antagonists against µ (CTOP: D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-ThrNH2, 6.25 nmol) and κ (Nor-BIN: nor-binaltorphimine, 10 nmol) opioid receptors were infused into the rostral ventromedial medulla (RVM) 10 min before a 30-min EA treatment at acupoint Huantiao (GB30) 1h 30 min post-CFA. PWL was measured before and 2.5 post-CFA. Both 10 Hz and 100 Hz EA-produced anti-hyperalgesia were blocked by intra-RVM µ, but not κ, receptor antagonists. Double immunofluorescence staining demonstrated that µ receptor-containing neurons were GABAnergic and that GABAa receptor-containing neurons were serotonergic in the RVM. The results demonstrated an involvement of RVM µ, but not κ, receptors in EA-produced anti-hyperalgesia. In summary, EA may induce release of endogenous endomorphins that activate µ opioid receptors in GABAnergic neurons to suppress the release of GABA. This removes the tonic inhibition of GABA on serotonergic neurons in the RVM, and activation of these serotonergic neurons inhibits pain. EA may be used as complementary treatment for inflammatory pain.


Asunto(s)
Electroacupuntura/métodos , Hiperalgesia/metabolismo , Hiperalgesia/terapia , Bulbo Raquídeo/metabolismo , Receptores Opioides kappa/fisiología , Receptores Opioides mu/fisiología , Animales , Modelos Animales de Enfermedad , Adyuvante de Freund/administración & dosificación , Adyuvante de Freund/toxicidad , Hiperalgesia/fisiopatología , Masculino , Bulbo Raquídeo/efectos de los fármacos , Bulbo Raquídeo/fisiopatología , Inhibición Neural/efectos de los fármacos , Inhibición Neural/fisiología , Inflamación Neurogénica/metabolismo , Inflamación Neurogénica/patología , Inflamación Neurogénica/terapia , Ratas , Ratas Sprague-Dawley , Receptores Opioides kappa/antagonistas & inhibidores , Receptores Opioides mu/antagonistas & inhibidores
11.
Domest Anim Endocrinol ; 40(3): 165-72, 2011 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-21194875

RESUMEN

Syndyphalin-33 (SD-33) increases feed intake in sheep and recently weaned pigs. To assess the effects of SD-33 on hypothalamic gene expression, hypothalami were collected from unweaned pigs (n=19; 21±3 d of age) on day 0. Remaining pigs received an intramuscular injection of 0.5 µmole/kg SD-33 (SD) or saline (VEH) and weaned into individual pens. On days 1, 4, and 7 after weaning, hypothalami were collected from subsets of pigs (n=8 or 9) within each treatment group. Expression of µ-opioid receptor (MOR) was less in SD pigs than in VEH pigs on day 1 and day 4, suggesting down-regulation of the receptor by SD-33. Expression of hypothalamic melanocortin 4 receptor (MC4R) at 1 d after weaning was increased in VEH pigs (but not SD pigs) relative to levels before weaning. Expression of AGRP was not significantly altered by weaning or treatment at 1 d after weaning. At 4 d after weaning, expression of AGRP was greater in SD pigs than in VEH pigs, but at day 7 expression was less in SD pigs than in VEH pigs. A strong positive correlation was noted between expression levels of MOR and MC4R across treatment and time. Treatment with SD-33 appeared to partially abrogate the effects of weaning on expression of two key appetite-regulating genes within 24 h. Effects of SD-33 appear to be mediated at least in part by the µ-opioid receptor and include actions on the melanocortinergic pathway.


Asunto(s)
Regulación de la Expresión Génica/fisiología , Hipotálamo/fisiología , Oligopéptidos/farmacología , Porcinos/fisiología , Proteína Relacionada con Agouti/genética , Proteína Relacionada con Agouti/fisiología , Animales , Animales Lactantes , Ingestión de Alimentos/fisiología , Femenino , Masculino , ARN Mensajero/química , ARN Mensajero/genética , Receptor de Melanocortina Tipo 4/genética , Receptor de Melanocortina Tipo 4/fisiología , Receptores Opioides mu/genética , Receptores Opioides mu/fisiología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Destete
12.
Alcohol Clin Exp Res ; 35(1): 166-74, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21039637

RESUMEN

BACKGROUND: Neurobiological theories of addiction have highlighted disruption in stress pathways as a central feature of addictive disorders, and pharmacological treatments targeting stress mechanisms hold great promise. This study examines genetic determinants of stress-induced and cue-induced craving in heavy drinkers by testing single-nucleotide polymorphisms (SNPs) of the corticotrophin-releasing hormone binding protein (CRH-BP) gene and the mu-opioid receptor (OPRM1) gene. METHODS: This study combines guided imagery stress exposure and in vivo alcohol cue exposure in a sample of 64 (23 women) non-treatment-seeking heavy drinkers. RESULTS: Analyses, uncorrected for multiple comparisons, revealed that a tag SNP of the CRH-BP gene (rs10055255) moderated stress-induced craving in this sample. The same SNP predicted greater affective responses to the stress manipulation, including greater levels of subjective tension and negative mood. The Asp40 allele of the OPRM1 was associated with greater cue-induced alcohol craving following the neutral imagery condition. CONCLUSIONS: These initial results extend recent preclinical and clinical findings implicating the CRH-BP in stress-related alcoholism and confirm the role of the Asp40 allele of the OPRM1 gene in reward-driven alcohol phenotypes. Human laboratory models of stress and cue-induced craving may be useful in pharmacotherapy development targeting dysregulation of stress systems. Larger studies are needed to validate these preliminary findings, which should also be extended to clinical samples.


Asunto(s)
Consumo de Bebidas Alcohólicas/genética , Alcoholismo/genética , Conducta Adictiva/genética , Proteínas Portadoras/genética , Receptores Opioides mu/genética , Estrés Psicológico , Adulto , Afecto , Consumo de Bebidas Alcohólicas/metabolismo , Consumo de Bebidas Alcohólicas/psicología , Alcoholismo/metabolismo , Alcoholismo/psicología , Alelos , Conducta Adictiva/metabolismo , Conducta Adictiva/psicología , Proteínas Portadoras/fisiología , Señales (Psicología) , Femenino , Humanos , Masculino , Persona de Mediana Edad , Polimorfismo de Nucleótido Simple , Receptores Opioides mu/fisiología , Recompensa , Adulto Joven
13.
Brain Res ; 1355: 61-9, 2010 Oct 08.
Artículo en Inglés | MEDLINE | ID: mdl-20696145

RESUMEN

Many studies have demonstrated the anti-nociceptive and anti-inflammatory effects of injecting bee venom (BV) into the Zusanli (ZSL) acupoint in rats. The present study was designed to determine whether the injection of other chemical irritants, such as formalin and complete Freund's adjuvant (CFA), into the ZSL acupoint can produce anti-nociceptive and anti-inflammatory effects in the BV pain model and to determine the possible mechanisms underlying these effects. First, the effects of injecting BV, formalin, CFA, or saline into the ZSL acupoint on intraplantar BV-induced persistent spontaneous pain, mechanical hyperalgesia, and inflammatory swelling of the injected paw were observed. BV, formalin, CFA, and saline injection into the ZSL acupoint significantly inhibited intraplantar BV-induced persistent spontaneous nociception (PSN) and mechanical hyperalgesia but had no effect on intraplantar BV-induced inflammatory swelling. Next, the effects of pretreatment with naloxone (5mg/kg, ip) or injection of 0.15% capsaicin into the ZSL acupoint on the anti-nociceptive effect of BV acupuncture (BVA) were observed. Pretreatment with naloxone had no effect on the BVA-induced anti-nociceptive effect, intraplantar BV-induced PSN, and mechanical hyperalgesia. Pretreatment with capsaicin produced partial blockage of the BVA-induced anti-nociceptive effect on PSN, but it had no effect on BVA-induced anti-nociception of mechanical hyperalgesia. These results suggest that (1) chemical irritant acupuncture produces the anti-nociceptive effect but not the anti-inflammatory effect in the BV pain model, and (2) chemical irritant acupuncture-induced analgesia is a common mechanism that is not specific to BV acupuncture. Our results also suggest that the BVA-induced anti-nociceptive mechanism is partially mediated by capsaicin-sensitive primary afferent fibers but not by endogenous mu opioid receptors in the BV pain model.


Asunto(s)
Analgesia por Acupuntura/métodos , Puntos de Acupuntura , Venenos de Abeja/farmacología , Irritantes/farmacología , Dolor/tratamiento farmacológico , Animales , Venenos de Abeja/uso terapéutico , Modelos Animales de Enfermedad , Interacciones Farmacológicas/fisiología , Inflamación/tratamiento farmacológico , Inflamación/etiología , Irritantes/uso terapéutico , Masculino , Dolor/fisiopatología , Ratas , Ratas Sprague-Dawley , Receptores Opioides mu/fisiología , Células Receptoras Sensoriales/fisiología
14.
Mol Pharmacol ; 77(5): 804-17, 2010 May.
Artículo en Inglés | MEDLINE | ID: mdl-20159949

RESUMEN

The pharmacology of G protein-coupled receptors can be influenced by factors such as constitutive receptor activation and Na(+) ions. In this study, we examined the coupling of natively and heterologously expressed nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptors with voltage-dependent Ca(2+) channels after exposure to four high-affinity NOP receptor blockers [[Nphe(1)Arg(14)Lys(15)]N/OFQ-NH(2) (UFP-101), 1-[1-(cyclooctylmethyl)-1,2,3,6-tetrahydro-5-(hydroxymethyl)-4-pyridinyl]-3-ethyl-1,3-dihydro-2H-benzimidazol-2-one (Trap-101), 1-benzyl-N-{3-[spiroisobenzofuran-1(3H),4'-piperidin-1-yl]propyl}pyrrolidine-2-carboxamide (compound 24), and N-(4-amino-2-methylquinolin-6-yl)-2-(4-ethylphenoxymethyl)benzamide hydrochloride (JTC-801)] in sympathetic neurons. The enhanced tonic inhibition of Ca(2+) currents in the absence of agonists, indicative of constitutively active NOP receptors in transfected neurons, was abolished after pretreatment with pertussis toxin. In control neurons, the four antagonists did not exert any effects when applied alone but significantly blocked the N/OFQ-mediated Ca(2+) current inhibition. Exposure of transfected neurons to UFP-101 resulted in partial agonist effects. In contrast, Trap-101, compound 24, and JTC-801 exerted inverse agonism, as measured by the loss of tonic Ca(2+) current inhibition. In experiments designed to measure the N/OFQ concentration-response relationship under varying Na(+) concentrations, a leftward shift of IC(50) values was observed after Na(+) exposure. Although similar N/OFQ efficacies were measured with all solutions, a significant decrease of Hill coefficient values was obtained with increasing Na(+) concentrations. Examination of the allosteric effects of Na(+) on heterologously overexpressed NOP receptors showed that the tonic Ca(2+) current inhibition was abolished in the presence of the monovalent cation. These results demonstrate that constitutively active NOP receptors exhibit differential blocker pharmacology and allosteric regulation by Na(+). Data are also presented demonstrating that heterologously expressed mu opioid receptors in sympathetic neurons are similarly modulated.


Asunto(s)
Neuronas/fisiología , Péptidos Opioides/farmacología , Receptores Opioides/genética , Sodio/farmacología , Sistema Nervioso Simpático/fisiología , Animales , Calcio/farmacología , Calcio/fisiología , ADN Complementario/genética , Electrofisiología/métodos , Antagonistas de Narcóticos , Neuronas/efectos de los fármacos , Plásmidos , Ratas , Receptores Opioides/agonistas , Receptores Opioides/efectos de los fármacos , Receptores Opioides mu/efectos de los fármacos , Receptores Opioides mu/genética , Receptores Opioides mu/fisiología , Transfección , Receptor de Nociceptina
15.
Eur J Pharmacol ; 610(1-3): 49-54, 2009 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-19285491

RESUMEN

The effect of new NOP receptor agonists and antagonists in the rat chronic constriction injury model was investigated. Intraperitoneally administered NOP receptor agonist SR14150 and antagonists SR16430 and SR14148, had no effect on mechanical allodynia when given alone. The nonselective NOP/mu-opioid receptor agonist SR16435, however, produced an anti-allodynic response, similar to morphine and reversible by naloxone. Notably, co-administration of the NOP receptor antagonists potentiated the anti-allodynic activity of both morphine and SR16435. Increased levels of the NOP receptor are implicated in the reduced efficacy of morphine in neuropathic pain. Our results suggest the utility of NOP receptor antagonists for potentiating opioid efficacy in chronic pain.


Asunto(s)
Analgésicos Opioides/farmacología , Indoles/farmacología , Mononeuropatías/tratamiento farmacológico , Morfina/farmacología , Dolor/tratamiento farmacológico , Fenalenos/farmacología , Receptores Opioides mu/antagonistas & inhibidores , Analgésicos Opioides/uso terapéutico , Animales , Evaluación Preclínica de Medicamentos , Interacciones Farmacológicas , Indoles/uso terapéutico , Ligandos , Masculino , Mononeuropatías/etiología , Dimensión del Dolor/métodos , Fenalenos/uso terapéutico , Ratas , Ratas Sprague-Dawley , Receptores Opioides , Receptores Opioides mu/agonistas , Receptores Opioides mu/fisiología , Nervio Ciático/cirugía , Receptor de Nociceptina
16.
J Neurosci ; 28(11): 2814-9, 2008 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-18337411

RESUMEN

Arousal and maintenance of a wake state is dependent on the hypothalamic hypocretin/orexin system. We found that hypocretin neurons are depressed by opiates, drugs of abuse that reduce cognitive alertness. Met-enkephalin (mENK), an endogenous opioid, and exogenous opiates such as morphine inhibited the hypocretin system by direct actions on the cell body that include reduced spike frequency, hyperpolarization, increased G-protein-coupled inwardly rectifying K(+) channel current, and attenuated calcium current, and indirectly through reducing excitatory synaptic tone by a presynaptic mechanism. CTAP (H-d-Phe-Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH(2)) and naloxone, antagonists of mu-opioid receptors, blocked mu agonist actions. In the absence of exogenous opioids, mu receptor antagonists enhanced activity of the hypocretin system, suggesting ongoing inhibition by endogenous receptors. Morphine pretreatment attenuated subsequent excitatory responses to hypocretin, suggesting a long-lasting depression caused by opiate exposure. Chronic exposure to morphine reduced subsequent responses to morphine and to mENK, but increased the response to opioid receptor antagonists. Together, these data are consistent with the view that the hypocretin system may be an important direct target for drugs of abuse, including opiates, that induce sedation and mental lethargy.


Asunto(s)
Nivel de Alerta/fisiología , Hipotálamo/fisiología , Péptidos y Proteínas de Señalización Intracelular/fisiología , Depresión Sináptica a Largo Plazo/fisiología , Neuropéptidos/fisiología , Receptores Opioides mu/fisiología , Animales , Nivel de Alerta/efectos de los fármacos , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Hipotálamo/efectos de los fármacos , Drogas Ilícitas/farmacología , Depresión Sináptica a Largo Plazo/efectos de los fármacos , Ratones , Ratones Transgénicos , Morfina/farmacología , Antagonistas de Narcóticos/farmacología , Orexinas , Receptores Opioides mu/agonistas , Receptores Opioides mu/antagonistas & inhibidores , Porcinos
17.
Anesth Analg ; 105(6): 1729-35, table of contents, 2007 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-18042875

RESUMEN

BACKGROUND: High-dose opioid therapy can precipitate seizures; however, the mechanism of such a dangerous adverse effect remains poorly understood. The aim of our study was to determine whether the neuroexcitatory activity of high-dose morphine is mediated by selective stimulation of opioid receptors. METHODS: Mice hippocampi were resected intact and bathed in low magnesium artificial cerebrospinal fluid to induce spontaneous seizure-like events recorded from CA1 neurons. RESULTS: Application of morphine had a biphasic effect on the recorded spontaneous seizure-like events. In a low concentration (10 microM), morphine depressed electrographic seizure activity. Higher morphine concentrations (30 and 100 microM) enhanced seizure activity in an apparent dose-dependent manner. Naloxone, a nonselective opiate antagonist blocked the proconvulsant action of morphine. Selective mu and kappa opiate receptor agonists and antagonists enhanced and suppressed the spontaneous seizure activity, respectively. On the contrary, delta opioid receptor ligands did not have an effect. CONCLUSIONS: The proseizure effect of morphine is mediated through selective stimulation of mu and kappa opiate receptors but not the activation of the delta receptor system. The observed dose-dependent mechanism of morphine neuroexcitation underscores careful adjustment and individualized opioid dosing in the clinical setting.


Asunto(s)
Morfina/toxicidad , Convulsiones/inducido químicamente , Convulsiones/fisiopatología , Animales , Animales Recién Nacidos , Relación Dosis-Respuesta a Droga , Hipocampo/efectos de los fármacos , Hipocampo/fisiología , Ratones , Ratones Endogámicos C57BL , Receptores Opioides kappa/agonistas , Receptores Opioides kappa/fisiología , Receptores Opioides mu/agonistas , Receptores Opioides mu/fisiología
18.
Chin Med J (Engl) ; 120(5): 394-9, 2007 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-17376310

RESUMEN

BACKGROUND: Preconditioning with repeated electroacupuncture (EA) could mimic ischemic preconditioning to induce cerebral ischemic tolerance in rats. The present study was designed to investigate whether mu (micro)-, delta (delta)- or kappa (kappa)-opioid receptors are involved in the neuroprotection induced by repeated EA preconditioning. METHODS: The rats were pretreated with naltrindole (NTI), nor-binaltorphimine (nor-BNI) or D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2 (CTOP), which is a highly selective delta-, kappa- or micro-opioid receptor antagonist respectively, before each EA preconditioning (30 minutes per day, 5 days). Twenty-four hours after the last EA treatment, the middle cerebral artery occlusion (MCAO) was induced for 120 minutes. The brain infarct volume was determined with 2, 3, 5-triphenyltetrazolium chloride staining at 24 hours after MCAO and compared with that in rats which only received EA preconditioning. In another experiment, the met-enkephalin-like immunoreactivity in rat brain was investigated by immunohistochemistry in both EA preconditioning and control rats. RESULTS: The EA preconditioning reduced brain infarct volume compared with the control rats (P = 0.000). Administration of both NTI and CTOP attenuated the brain infarct volume reduction induced by EA preconditioning, presenting with larger infarct volume than that in the EA preconditioning rats (P < 0.001). But nor-BNI administration did not block the infarct volume reduction induced by EA preconditioning, presenting with smaller infarct volume than the control group rats (P = 0.000). The number of met-enkephalin-like immunoreactivity positive neurons in the EA preconditioning rats was more than that of the control rats (P = 0.000). CONCLUSION: Repeated EA preconditioning stimulates the release of enkephalins, which may bind delta- and micro-opioid receptors to induce the tolerance against focal cerebral ischemia.


Asunto(s)
Isquemia Encefálica/prevención & control , Electroacupuntura , Precondicionamiento Isquémico , Receptores Opioides delta/fisiología , Receptores Opioides mu/fisiología , Animales , Encefalina Metionina/análisis , Inmunohistoquímica , Masculino , Naltrexona/análogos & derivados , Naltrexona/farmacología , Ratas , Ratas Sprague-Dawley , Somatostatina/análogos & derivados , Somatostatina/farmacología
19.
J Neurophysiol ; 97(2): 1058-67, 2007 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-17151221

RESUMEN

The most common single-nucleotide polymorphism (SNP) of the human mu-opioid receptor (hMOR) gene occurs at position 118 (A118G) and results in substitution of asparagine to aspartate at the N-terminus. The purpose of the present study was to compare the pharmacological profile of several opioid agonists to heterologously expressed hMOR and N-type Ca(2+) channels in sympathetic neurons. cDNA constructs coding for wild-type and mutant hMOR were microinjected in rat superior cervical ganglion neurons and N-type Ca(2+) channel modulation was investigated using the whole cell variant of the patch-clamp technique. Concentration-response relationships were generated with the following selective MOR agonists: DAMGO, morphine, morphine-6-glucuronide (M-6-G), and endomorphin I. The estimated maximal inhibition for the agonists ranged from 52 to 64% for neurons expressing either hMOR subtype. The rank order of potencies for estimated EC(50) values (nM) in cells expressing wild-type hMOR was: DAMGO (31) >> morphine (76) congruent with M-6-G (77) congruent with endomorphin I (86). On the other hand, the rank order in mutant-expressing neurons was: DAMGO (14) >> morphine (39) >> endomorphin I (74) congruent with M-6-G (82), with a twofold leftward shift for both DAMGO and morphine. The DAMGO-mediated Ca(2+) current inhibition was abolished by the selective MOR blocker, CTAP, and by pertussis toxin pretreatment of neurons expressing either hMOR subtype. These results suggest that the A118G variant MOR exhibits an altered signal transduction pathway and may help explain the variability of responses to opiates observed with carriers of the mutant allele.


Asunto(s)
Canales de Calcio Tipo N/genética , Canales de Calcio Tipo N/fisiología , Polimorfismo de Nucleótido Simple/genética , Polimorfismo de Nucleótido Simple/fisiología , Receptores Opioides mu/genética , Receptores Opioides mu/fisiología , Analgésicos Opioides/metabolismo , Analgésicos Opioides/farmacología , Animales , Separación Celular , ADN Complementario/genética , Relación Dosis-Respuesta a Droga , Electrofisiología , Encefalina Ala(2)-MeFe(4)-Gli(5)/farmacología , Humanos , Masculino , Microinyecciones , Morfina/metabolismo , Morfina/farmacología , Derivados de la Morfina/farmacología , Mutación/fisiología , Neuronas/fisiología , Técnicas de Placa-Clamp , Toxina del Pertussis/farmacología , Ratas , Ratas Wistar , Ganglio Cervical Superior/citología , Ganglio Cervical Superior/fisiología
20.
Eur J Pharmacol ; 549(1-3): 63-70, 2006 Nov 07.
Artículo en Inglés | MEDLINE | ID: mdl-16978601

RESUMEN

7-hydroxymitragynine, a constituent of the Thai herbal medicine Mitragyna speciosa, has been found to have a potent opioid antinociceptive effect. In the present study, we investigated the mechanism of antinociception and the inhibitory effect on gastrointestinal transit of 7-hydroxymitragynine, and compared its effects with those of morphine. When administered subcutaneously to mice, 7-hydroxymitragynine produced antinociceptive effects about 5.7 and 4.4 times more potent than those of morphine in the tail-flick (ED50=0.80 mg/kg) and hot-plate (ED50=0.93 mg/kg) tests, respectively. The antinociceptive effect of 7-hydroxymitragynine was significantly blocked by the mu1/mu2-opioid receptor antagonist beta-funaltrexamine hydrochloride (beta-FNA) and the mu1-opioid receptor-selective antagonist naloxonazine in both tests. Thus, 7-hydroxymitragynine acts predominantly on mu-opioid receptors, especially on mu1-opioid receptors. Isolated tissue studies further supported its specificity for the mu-opioid receptors. Further, 7-hydroxymintragynine dose-dependently (ED50=1.19 mg/kg, s.c.) and significantly inhibited gastrointestinal transit in mice, as morphine does. The inhibitory effect was significantly antagonized by beta-FNA pretreatment, but slightly antagonized by naloxonazine. The ED50 value of 7-hydroxymitragynine on gastrointestinal transit was larger than its antinociceptive ED50 value. On the other hand, morphine significantly inhibits gastrointestinal transit at a much smaller dose than its antinociceptive dose. These results suggest that mu-opioid receptor mechanisms mediate the antinociceptive effect and inhibition of gastrointestinal transit. This compound induced more potent antinociceptive effects and was less constipating than morphine.


Asunto(s)
Analgésicos/farmacología , Tránsito Gastrointestinal/efectos de los fármacos , Mitragyna/química , Receptores Opioides mu/fisiología , Alcaloides de Triptamina Secologanina/farmacología , Analgésicos/química , Analgésicos/aislamiento & purificación , Analgésicos Opioides/farmacología , Animales , Relación Dosis-Respuesta a Droga , Tránsito Gastrointestinal/fisiología , Cobayas , Medicina de Hierbas , Íleon/efectos de los fármacos , Íleon/fisiología , Masculino , Ratones , Estructura Molecular , Morfina/farmacología , Contracción Muscular/efectos de los fármacos , Naltrexona/análogos & derivados , Naltrexona/farmacología , Antagonistas de Narcóticos/farmacología , Narcóticos/química , Narcóticos/aislamiento & purificación , Narcóticos/farmacología , Dolor/fisiopatología , Dolor/prevención & control , Dimensión del Dolor/métodos , Receptores Opioides mu/antagonistas & inhibidores , Alcaloides de Triptamina Secologanina/química , Alcaloides de Triptamina Secologanina/aislamiento & purificación , Tailandia , Conducto Deferente/efectos de los fármacos , Conducto Deferente/fisiología
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