Aqueous extracts from the selected hyperaccumulators used as soil additives significantly improve accumulation capacity of Solanum nigrum L. for Cd and Pb.

The effects of soil treatment with aqueous extracts from three hyperaccumulators on Cd and Pb accumulation by Solanum nigrum L. were determined. The stem (S-RG) and leaf extracts (L-RG) of Rorippaglobosa (Turcz.) Thell., and stem extract (S-BP) of Bidens pilosa L. significantly enhanced Cd and Pb total accumulation capacity of S. nigrum compared to control (by 44 %, 47 %, and 29 % for Cd and by 28 %, 28 % and 21 % for Pb, respectively), while EDTA caused its 9 % and 15 % decrease due to the plant biomass reduction (by 33 %). The leaching experiments reflected affinity of additives to metal mobilization in soils. The concentrations of total organic acid in S-RG, L-RG and S-BP were the highest among studied extracts, which besides the beneficial effect on the soil environment (microbe number and enzyme activities), may be partial reasons of strong promotion of S. nigrum accumulation capacity for Cd and Pb. It was shown that hyperaccumulation properties of a plant are not a prerequisite of enhancing effect of the plant-based soil additive on the metal accumulation capacity of the target living hyperaccumultor. The plant-based chelators were found to be promising candidates for EDTA and other chemicals replacement in promoting efficient and environmentally safe phytoremediation.

Profiling of glucosinolates and flavonoids in Rorippa indica (Linn.) Hiern. (Cruciferae) by UHPLC-PDA-ESI/HRMS(n).

An UHPLC-PDA-ESI/HRMS(n) profiling method was used to identify the glucosinolates and flavonoids of Rorippa indica (Cruciferae), a wild vegetable and Chinese herb used to treat cough, diarrhea, and rheumatoid arthritis. Thirty-three glucosinolates, more than 40 flavonol glycosides, and 18 other phenolic and common organic compounds were identified. The glucosinolates and polyphenols were separated by UHPLC. High-resolution deprotonated molecules provided high accuracy mass values that were used to determine formulas and provide putative identification of the glucosinolates and flavonoids. The fragments from multistage mass spectrometry were used to elucidate the structures. The concentrations of the main components were based on UV peak areas and molar relative response factors with a single calibration standard. This study found this plant to be a rich source for glucosinolates, containing 24 new glucosinolates, including 14 glucosylated glucosinolates that were previously unidentified.