Biofilm and suspension-based cultivation of microalgae to treat anaerobic digestate food effluent (ADFE).

Anaerobic digestion of organic waste produces effluent (ADE) that requires further treatment. Biofilm-based microalgal cultivation is a favoured approach to ADE treatment. This study compared Chlorella sp. MUR 268 and Scenedesmus sp. MUR 269 in biofilm and suspension cultures to treat anaerobic digestate food effluent (ADFE). Chlorella sp. MUR 268 biofilm had significantly higher biomass (50.38 g m-2) than Scenedesmus sp. biofilm (9.39 g m-2). Conversely, Scenedesmus sp. yielded 1.5 times more biomass (1.2 g L-1) than Chlorella sp. in suspension. Chlorella sp. biofilm had 49.3 % higher areal productivity than suspension, while Scenedesmus sp. showed 87.3 % higher areal growth in suspension. Chlorella sp. MUR 268 and Scenedesmus sp. MUR 269 significantly removed nutrients in ADFE. In suspension, COD, ammoniacal nitrogen, and phosphate were reduced to 94.9, 5.2, and 5.98 mg L-1 for Chlorella sp. MUR 268, and 245, 2.89, and 3.22 mg L-1 for Scenedesmus sp. MUR 269, respectively. In biofilm, Chlorella sp. MUR 268 achieved reductions to 149.9, 1.16, and 3.57 mg L-1, while Scenedesmus sp. MUR 269 achieved 100.2, 6.9 and 2.07 mg L-1. Most of these values are below the recommended effluent discharge standard, highlighting the efficacy of this system in ADFE treatment. Biofilm cultures fixed 68-81 % of removed nitrogen in biomass, while in suspension, only 55-71 % ended in the biomass. Chlorella sp. MUR 268 biofilm fixed 88 % of removed phosphorus, while Scenedesmus sp. MUR 269 suspension fixed more phosphorus (55 %) than the biofilm counterpart (34 %). This biofilm design offers advantages like simplified, cost-effective operation, easy biomass recovery, and reduced water usage.

Carbon-negative and high-rate nutrient recovery from municipal wastewater using mixotrophic Scenedesmus acuminatus.

The efficiency of mixotrophic microalgae in enhancing the recovery of waste nutrients has been well established; however, the recovery rate is crucial in meeting the needs of field applications. This study evaluated the impact of media characteristics on nutrient recovery under mixotrophic conditions. The mixotrophic N recovery rate with S. acuminatus in modified BG-11 reached 2.59 mg L-1h-1. A mixotrophic growth optimization strategy was applied to achieve a high-rate nutrient recovery from municipal wastewater treatment plant effluents. The contribution of waste chemical oxygen demand (COD) to nutrient recovery was assessed using secondary effluent (SE) under heterotrophy. The results highlighted a significant increase in total nitrogen (TN) and total phosphorus (TP) recovery rates when glucose was supplied, indicating the additional carbon requirements for efficient nutrient recovery. The TN and TP recovery rates under mixotrophic conditions with the addition of trace metals and high cell density were enhanced by 91.94% and 92.53%, respectively, resulting in recovery rates of 3.43 mg L-1h-1 and 0.30 mg L-1h-1. The same conditions were used for nutrient recovery from primary effluent (PE), and the results were more satisfactory as the TN and TP recovery rates reached 4.79 and 0.55 mg L-1h-1, respectively. Additionally, the study estimated the carbon footprints (C-footprints) and areal footprints of mixotrophy-based nitrogen recovery. The findings revealed carbon footprints and areal footprints of -15.93 ± 4.57 tCO2e t-1 N recovery and 0.53 ± 0.19 m3 m-2d-1 wastewater, respectively. This high-rate nutrient recovery, achieved under a carbon-negative (C-negative) budget through mixotrophy, presents a novel strategy for efficiently recovering resources from municipal wastewater, thus facilitating resource recycling and ensuring environmental sustainability.

Catalytic flash pyrolysis of Scenedesmus sp. post-extraction residue using low-cost HZSM-5 catalyst with the perspective to produce renewable aromatic hydrocarbons.

Recovering renewable chemicals from de-fatted microalgal residue derived from lipid extraction within the algal-derived biofuel sector is crucial, given the rising significance of microalgal-derived biodiesel as a potential substitute for petroleum-based liquid fuels. As a circular economy strategy, effective valorization of de-fatted biomass significantly improves the energetic and economic facets of establishing a sustainable algal-derived biofuel industry. In this scenario, this study investigates flash catalytic pyrolysis as a sustainable pathway for valorizing Scenedesmus sp. post-extraction residue (SPR), potentially yielding a bio-oil enriched with upgraded characteristics, especially renewable aromatic hydrocarbons. In the scope of this study, volatile products from catalytic and non-catalytic flash pyrolysis were characterized using a micro-furnace type temperature programmable pyrolyzer coupled with gas chromatographic separation and mass spectrometry detection (Py-GC/MS). Flash pyrolysis of SPR resulted in volatile products with elevated oxygen and nitrogen compounds with concentrations of 46.4% and 26.4%, respectively. In contrast, flash pyrolysis of lyophilized microalgal biomass resulted in lower concentrations of these compounds, with 40.9% oxygen and 17.3% nitrogen. Upgrading volatile pyrolysis products from SPR led to volatile products comprised of only hydrocarbons, while completely removing oxygen and nitrogen-containing compounds. This was achieved by utilizing a low-cost HZSM-5 catalyst within a catalytic bed at 500 °C. Catalytic experiments also indicate the potential conversion of SPR into a bio-oil rich in monocyclic aromatic hydrocarbons, primarily BETX, with toluene comprising over one-third of its composition, thus presenting a sustainable pathway for producing an aromatic hydrocarbon-rich bio-oil derived from SPR. Another significant finding was that 97.8% of the hydrocarbon fraction fell within the gasoline range (C5-C12), and 35.5% fell within the jet fuel range (C8-C16). Thus, flash catalytic pyrolysis of SPR exhibits significant promise for application in drop-in biofuel production, including green gasoline and bio-jet fuel, aligning with the principles of the circular economy, green chemistry, and bio-refinery.

Polystyrene nanoplastics alter the ecotoxicological effects of diclofenac on freshwater microalgae Scenedesmus obliquus.

Due to the escalating risk of plastic pollution, nanoplastics have attracted considerable attention in the recent past. They can co-exist and interact with other contaminants like pharmaceuticals in the aquatic environment. Therefore, it is pertinent to understand how these pollutants interact with one another in the ecosystem. The current study examined the individual and combined effects of fluorescent polystyrene nanoplastics (FNPs) and diclofenac (DCF) on Scenedesmus obliquus using a full factorial design. The toxicity of S. obliquus significantly increased in a dose-dependent manner upon exposure to pristine forms of DCF and FNPs. The major cause of individual toxicity of DCF and FNPs in S. obliquus was oxidative stress. In the combined toxicity tests when FNPs (0.01, 0.1, and 1 mg L-1) and DCF (1 mg L-1) were mixed, a synergistic effect was noted compared to the respective pristine FNPs. However, when the DCF concentration in the mixture was decreased to 0.25 mg L-1, the combined toxicity with FNPs (0.01, 0.1, and 1 mg L-1) reduced indicating an antagonistic effect. The independent action model also showed an antagonistic effect for low-dose combinations of DCF and a synergistic effect for high-dose combinations. The estimation of oxidative stress parameters, antioxidant enzyme activity, and photosynthetic pigment content in the algae further validated the cytotoxicity data. The mean hydrodynamic diameter and surface charge analyses further indicated that the colloidal stability of the FNPs in the medium was affected when they were combined with DCF. The key reason for differences in the cytotoxicity of combinations could be observed variations in the aggregation of FNPs and differential adsorption patterns of DCF on the FNPs. These factors efficiently altered cell-particle interactions in the mixture demonstrating a hormesis effect. Thus, this current study highlighted the hazardous nature of the nanoplastics and their co-exposure risks with pharmaceuticals on microalgae in freshwater environments.

Growth of Scenedesmus obliquus on anaerobic soybean wastewater using different wasted organics for high biomass production and nutrients recycling.

The microalgae culture in mixing sewage with different characteristics may significantly improve biomass production and nutrients recycling efficiency. In this study, three waste organic wastewater including molasses, alcohol and glycerol wastewater were mixed with anaerobic soybean wastewater as mediums for microalgae culture. The optimal mixture of molasses, alcohol and glycerol wastewater was at an initial carbon-nitrogen ratio of 7:1, 5:1 and 10:1, improving biomass production by 60.4%, 31.3% and 68.7%, respectively. The removal efficiencies of organics, ammonia nitrogen and phosphorus at optimal mixture were 54.8-62.4%, 79.5-99.1% and 49.3-61.5%, and the removal rates increased by 340-630%, 27.5-66.3% and 36.3-70.2% compared to the blank culture. In addition, the culture in mixed wastewater increased lipids contrast by 0.7-1.3 times, while achieving higher saturation in fatty acids. The results suggested that microalgae culture using mixed wastewater was a strategy for high biomass production and nutrients recycling efficiency.

Long-term assessment of the nutrient recovery capacity and biomass productivity of Scenedesmus almeriensis in raceway reactors using unprocessed urban wastewater.

The present work aims to assess the treatment of unprocessed urban wastewater using the microalga Scenedesmus almeriensis. Two 12 m3 raceway reactors, one supplemented by wastewater and the second by chemical fertilizer, operating outdoors in a semi-continuous mode, were used for eight months. Results suggested that S. almeriensis can be produced in wastewater without affecting the photosynthetic apparatus reaching a productivity of 13 g·m-2·day-1 on average in both the systems. Furthermore, the nutrient content in terms of nitrogen, phosphorous and chemical oxygen demand of the wastewater was reduced under the European limitations during most of the period, with an average removal rate of 2.2, 0.2 and 3.0 g·m-2·day-1 respectively. Therefore, raceways demonstrated a high potential for microalgal production and successful biotreatment, proving robust and reliable. Finally, the effect of environmental conditions on biomass productivity of the clean system was evaluated in a model with high accuracy (R2 = 0.9, p = 0.0002).

Growth of Scenedesmus dimorphus in swine wastewater with versus without solid-liquid separation pretreatment.

Scenedesmus dimorphus was cultivated in raw and pretreated swine wastewater (SW) with 6-L photobioreactors (PBRs) to investigate the effect of solid-liquid separation on algal growth. The same aerated PBRs containing no algae were used as control. Moderate COD and nitrogen removal from the SW was achieved with the algal PBRs. However, compared to the control reactors, they offered no consistent treatment boost. Improved algal growth occurred in the pretreated SW, as measured by maximum algal cell count (3202 ± 275 × 106 versus 2286 ± 589 × 106 cells L-1) and cell size. The enhanced algal growth in the pretreated SW resulted in relatively high nitrogen (5.7 %) and organic matter contents in the solids harvested at the end of cultivation experiments, with ∼25.6 % of nitrogen in the SW retained in the solids and ∼9.1 % absorbed by algae. The pretreatment also resulted in elevated phosphorus removal. This study is anticipated to foster the development of microalgae-based SW treatment processes.

Evaluation of the fertilizer potential of Chlorella vulgaris and Scenedesmus obliquus grown in agricultural drainage water from maize fields.

Producing microalgae with agricultural drainage water (ADW) allows recycling water and nutrients, with the production of a biofertilizer, avoiding receiving waters' contamination. Chlorella vulgaris and Scenedesmus obliquus were cultivated using ADW and standard media supplementation and presented higher productivities, relatively to the control industrial growth medium (using freshwater). Selected strains were grown outdoors in pilot flat panel photobioreactors, reaching 2.20 g L-1 for S. obliquus and 1.15 g L-1 for C. vulgaris, and degrading herbicides in the ADW to non-quantifiable concentrations. The potential of the C. vulgaris and S. obliquus suspensions to replace 50% of nitrogen (N) mineral fertilization of lettuce (0.5 g pot-1) was evaluated through a pot trial, also using a 2-times (1.0 g pot-1) and 5-times (2.5 g pot-1) higher dose, applied 31 days before lettuce transplanting. Even the lower dose of N, applied via C. vulgaris or S. obliquus suspensions, was able to provide significantly higher lettuce fresh matter yield, relatively to the mineral fertilized control. Soil enzymatic activities were improved, with significantly higher dehydrogenase, ß-glucosidase, and acid phosphatase activities for the 2.5 g pot-1 dose, more marked for S. obliquus, which was also able to increase soil organic matter content. Both the non-fertilized control and microalgae fertilized pots led to similar soil electrical conductivities, 3-fold lower than in the N-mineral fertilized pots, evidencing the capacity of microalgae fertilizers to avoid soil secondary salinization. Results suggest benefits from using ADW from maize cultivation to produce C. vulgaris or S. obliquus suspensions, that can be further used as liquid organic slow-release fertilizer.

The growth and lipid accumulation of Scenedesmus quadricauda under nitrogen starvation stress during xylose mixotrophic/heterotrophic cultivation.

In order to conquer the block of high cost and low yields which limit to realize the commercialization of microalgal biodiesel, the mixotrophic and heterotrophic cultivation of Scenedesmus quadricauda FACHB-1297 fed on xylose was separately studied employing six forms of media: phosphorus sufficient, phosphorus restricted, and phosphorus starvation were combined with nitrogen sufficient and nitrogen starvation conditions. The maximum lipid content (about 41% of dry weight) was obtained on the 5th day (heterotrophic cultivation) and 8th day (mixotrophic cultivation) under the nitrogen starved and phosphorus sufficient (N0&P) conditions, which was about twofold in comparison to the final lipid content on the sufficient nitrogen condition (control). Under mixotrophic and heterotrophic modes, the highest lipid production was achieved in the N0&P trial, with the value of 274.96 mg/L and 193.77 mg/L, respectively. Xylose utilization rate of 30-96% under heterotrophic modes was apparently higher than that of 20-50% in mixotrophic modes. In contrast, phosphorus uptake rate of 100% under mixotrophic cultivation was significantly more than that of 60-90% in heterotrophic cultivation. Furthermore, under the condition of heterotrophic cultivation using xylose as a carbon source, the phosphorus had a positive impact on microalgae cell synthesis and the lipid content enhanced with the augmentation in phosphorus concentrations. We suggested that sufficient phosphorus should be supplied for obtaining higher microalgal lipid production in the lack of nitrogen under xylose heterotrophic/mixotrophic condition. This was a highly effective way to obtain efficient microalgae lipid production.

Revealing the role of phosphorus supply on the phosphorus distribution and lipid production in Scenedesmus obliquus UTEX 393 during nitrogen starvation.

Microalgal-based processes offer promise for addressing two sustainability challenges: recovering phosphorus (P) from wastewater and producing biofuel feedstock. This study investigated the role of phosphorus supply on microalgal growth, lipid yield, and P distribution for Scenedesmus during nitrogen starvation. Extracellular polymeric substances and intracellular polymeric substances were the most important pools for inorganic phosphorus (IP) and organic phosphorus (OP), respectively. The main P pool for microalgae with low phosphorus supply was EPS, which accounted for 57 % of the total biomass phosphorus; while under high P concentrations, 79 % of the phosphorus was stored in IPS. A high concentration of orthophosphate stimulated rapid P uptake as IP and promoted the transformation of IP to OP associating with biomass synthesis. The highest P content of microalgal biomass was 6.5 % of dry weight when the phosphorus concentration in medium was 113 mg/L, and the OP content was 4.9 % of dry weight. High phosphate-P enhanced the biomass's lipid content by 60 %, and the distribution of fatty acid methyl esters was not altered by P concentrations. Collectively, high phosphate-P availability could promote microalgal biomass synthesis, lipid production and P accumulation.

Simultaneous bioremediation of Disperse orange-2RL Azo dye and fatty acids production by Scenedesmus obliquus cultured under mixotrophic and heterotrophic conditions.

Several types of green photosynthetic microalgae can grow through the process of heterotrophic growth in the dark with the help of a carbon source instead of the usual light energy. Heterotrophic growth overcomes important limitations in the production of valuable products from microalgae, such as the reliance on light, which complicates the process, raises costs, and lowers the yield of potentially useful products. The present study was conducted to explore the potential growth of green microalga Scenedesmus obliquus under mixotrophic and heterotrophic conditions utilizing Disperse orange 2RL Azo dye as a carbon source to produce a high lipid content and the maximum dye removal percentage. After 7 days of algal growth with dye under mixotrophic and heterotrophic conditions with varying pH levels (5, 7, 9, and 11), KNO3 concentrations (1, 1.5, 2, and 3 g/L), and dye concentrations (20, 40, and 60 ppm); dye removal percentage, algal dry weight, and lipid content were determined. The results showed that the highest decolorization of Disperse orange 2RL Azo dye (98.14%) was attained by S. obliquus in heterotrophic medium supplemented with glucose at the optimal pH 11 when the nitrogen concentration was 1 g/L and the dye concentration was 20 ppm. FT-IR spectroscopy of the dye revealed differences in peaks position and intensity before and after algal treatment. S. obliquus has a high concentration of oleic acid, which is enhanced when it is grown with Disperse orange 2RL Azo dye, making it ideal for production of high-quality biodiesel. In general, and in the vast majority of instances, heterotrophic cultivation is substantially less expensive, easier to set up, and requires less maintenance than mixotrophic cultivation. Heterotrophic cultivation allows for large-scale applications such as separate or mixed wastewater treatment along with biofuel production.

The comprehensive effects of aluminum oxide nanoparticles on the physiology of freshwater microalga Scenedesmus obliquus and it's phycoremediation performance for the removal of sulfacetamide.

Nanoparticles are inevitable byproducts of modern industry. However, the environmental impacts arising from industrial applications of nanoparticles are largely under-reported. This study evaluated the ecotoxicological effects of aluminum oxide nanoparticles (Al2O3NP) and its influence on sulfacetamide (SA) biodegradation by a freshwater microalga, Scenedesmus obliquus. Although Al2O3NP showed limited toxicity effect on S. obliquus, we observed the toxicity attenuation aspect of Al2O3NP in a mixture of sulfacetamide on microalgae. The addition of 100 mg L-1 of Al2O3NP and 1 mg L-1 of SA reduced total chlorophyll by 23.3% and carotenoids by 21.6% in microalgal compared to control. The gene expression study demonstrated that ATPF0C, Lhcb1, HydA, and psbA genes responsible for ATP synthesis and the photosynthetic system were significantly downregulated, while the Tas gene, which plays a major role in biodegradation of organic xenobiotic chemicals, was significantly upregulated at 1 and 100 mg L-1 of Al2O3NP. The S. obliquus removed 16.8% of SA at 15 mg L-1 in 14 days. However, the removal was slightly enhanced (18.8%) at same concentration of SA in the presence of 50 mg L-1 Al2O3NP. This result proves the stability of sulfacetamide biodegradation capacity of S. obliquus in the presence of Al2O3NP co-contamination. The metabolic analysis showed that SA was degraded into simpler byproducts such as sulfacarbamide, sulfaguanidine, sulfanilamide, 4-(methyl sulfonyl)aniline, and N-hydroxy-benzenamine which have lower ecotoxicity than SA, demonstrating that the ecotoxicity of sulfacetamide has significantly decreased after the microalgal degradation, suggesting the environmental feasibility of microalgae-mediated wastewater technology. This study provides a deeper understanding of the impact of nanoparticles such as Al2O3NP on aquatic ecosystems.

Selenium recovery from wastewater by the green microalgae Chlorella vulgaris and Scenedesmus sp.

Selenium (Se) is an important element for many living organisms and its supplementation may be needed in food, feed, and soil to make up for its deficiency. At the same time, high selenium concentrations can harm the environment, thus its management in sewage and the study of its removal from waste streams are important. Microalgae-based systems may be used for wastewater treatment and nutrients recovery, while producing biomass for bioproducts or bioenergy. In this study, Chlorella vulgaris and Scenedesmus sp. grown in urban wastewater with different selenium concentrations (50-1000 µg Se/L) were evaluated for their resistance and selenium removal/recovery efficiency. Chlorella vulgaris and Scenedesmus sp. were able to remove up to 43 and 52 % of Se from wastewater, respectively. Chlorella vulgaris accumulated up to 323 mgSe/kg DW (in urban wastewater with 1000 µg Se/L). The Se-rich biomass produced may be applied to the supplementation of animal feed or used for biofortification of crops.

Self-regulation mechanism difference of Chlorella vulgaris and Scenedesmus obliquus in toxic sludge extract caused by hydroquinone biodegradation.

Chlorella vulgaris (C. vulgaris) and Scenedesmus obliquus (S. obliquus) were compared to remove toxicity under conditions of sludge extract cultivation for 30 days. The toxicity of sludge extract, the growth characteristics, photosynthetic pigment, superoxide dismutase (SOD) enzyme and catalase (CAT) enzyme activities of the two microalgae were studied by contrast. The results showed that small molecular organic matter (<500 Da) was more easily utilized by microalgae. The toxicity in the toxic group of C. vulgaris and S. obliquus on the 30th day decreased to 56.8 ± 1.2% and 60.7 ± 2.8%, respectively. In the toxic group, the maximal SOD enzyme activity of C. vulgaris and S. obliquus were 2.02 U/mg proteins and 8.21 U/mg proteins, respectively, demonstrating that toxicity caused more oxidative damage to S. obliquus than to C. vulgaris. Proteomics analysis revealed that C. vulgaris mainly regulates energy synthesis and distribution primarily through sugar metabolism, and biomass synthesis primarily through carbon metabolism, whereas S. obliquus mainly regulates energy synthesis and distribution primarily through sugar metabolism and oxidative phosphorylation, resulting in sludge toxicity stress regulation.

Adverse role of colonial morphology and favorable function of microcystins for Microcystis to compete with Scenedesmus.

In eutrophic freshwaters, Microcystis usually becomes dominant in phytoplankton communities due to the synergistic effects of its special eco-physiological traits and environmental factors. Colonial morphology can protect Microcystis from zooplankton grazing, which indirectly favors Microcystis to outcompete other phytoplankton, although the colonial form is not conducive to the absorption of nutrients. Moreover, unicellular Microcystis usually has competitive advantages over other phytoplankton due to its efficient absorption capacity for nutrients and releasing microcystins. However, the consequence of direct competition between toxic colonial Microcystis and green algae without external grazing pressure still remained unknown. In this study, the competition between toxic colonial Microcystis aeruginosa and a common green alga Scenedesmus obliquus was explored. Results showed that: (1) colonial M. aeruginosa had a higher requirement for key macro-nutrient phosphorus than S. obliquus, and thus its population declined and was replaced by S. obliquus eventually; (2) microcystins released by colonial M. aeruginosa inhibited the photosynthetic activity and growth of S. obliquus at early stage of the competition; (3) the photosynthetic potential of colonial M. aeruginosa was stimulated in response to the competitive stress from S. obliquus, although the population of colonial M. aeruginosa declined eventually; (4) microcystin production of colonial M. aeruginosa was enhanced by phosphorus limitation due to S. obliquus competition and was positively related to photosynthetic potential of colonial M. aeruginosa. These results indicated that, in the absence of complex natural environment, colonial Microcystis cannot outcompete Scenedesmus in a pure competition, although microcystins can play a favorable role in the competition, which clarified the opposite role of colonies and microcystins in the competition of colonial Microcystis against other phytoplankton.

Microalgae and Bacteria Interaction-Evidence for Division of Diligence in the Alga Microbiota.

Microalgae are one of the most dominant forms of life on earth that is tightly associated with a distinct and specialized microbiota. We have previously shown that the microbiota of Scenedesmus quadricauda harbors less than 10 distinct microbial species. Here, we provide evidence that dominant species are affiliated with the genera of Variovorax, Porphyrobacter, and Dyadobacter. Experimental and transcriptome-based evidence implies that within this multispecies interaction, Dyadobacter is a key to alga growth and fitness and is highly adapted to live in the phycosphere. While presumably under light conditions the alga provides the energy source to the bacteria, Dyadobacter produces and releases mainly a large variety of polysaccharides modifying enzymes. This is coherent with high-level expression of the T9SS in alga cocultures. The transcriptome data further imply that quorum-quenching proteins (QQ) and biosynthesis of vitamins B1, B2, B5, B6, and B9 are expressed by Dyadobacter at high levels in comparison to Variovorax and Porphyrobacter. Notably, Dyadobacter produces a significant number of leucine-rich repeat (LRR) proteins and enzymes involved in bacterial reactive oxygen species (ROS) tolerance. Complementary to this, Variovorax expresses the genes of the biosynthesis of vitamins B2, B5, B6, B7, B9, and B12, and Porphyrobacter is specialized in the production of vitamins B2 and B6. Thus, the shared currency between partners are vitamins, microalgae growth-promoting substances, and dissolved carbon. This work significantly enlarges our knowledge on alga-bacteria interaction and demonstrates physiological investigations of microalgae and associated bacteria, using microscopy observations, photosynthetic activity measurements, and flow cytometry. IMPORTANCE The current study gives a detailed insight into mutualistic collaboration of microalgae and bacteria, including the involvement of competitive interplay between bacteria. We provide experimental evidence that Gram-negative bacteria belonging to the Dyadobacter, Porphyrobacter, and Variovorax are the key players in a Scenedesmus quadricauda alga-bacteria interaction. We impart strong evidence that Dyadobacter produces and releases polysaccharides degradation enzymes and leucine-rich repeat proteins; Variovorax supplies the consortium with auxins and vitamin B12, while Porphyrobacter produces a broad spectrum of B vitamins. We show not only that the microalgae collaborate with the bacteria and vice versa but also that the bacteria interact with each other via quorum-sensing and secretion system mechanisms. The shared currency between partners appears to be vitamins, microalgae growth-promoting substances, and dissolved carbon.

Effects of different concentrations of CO2 on Scenedesmus obliquus to overcome sludge extract toxicity and accumulate biomass.

Large amounts of toxic excess sludge as well as high concentrations of carbon dioxide can be produced in coal-gasification industry. Microalgae has huge potential in the use of nutrients, the removal of toxic organic matter in excess sludge and CO2 fixation. At the same time, the cultivation of the microalgae and the accumulation of high-quality biomass are also the key problems of concern. In this study, the growth and biomass synthesis of Scenedesmus obliquus cultured in sludge extract under 0%-15% (v/v) CO2 were investigated. Results indicated that the highest microalgae biomass yield of 1.609 ± 0.012 g/L can be achieved under 15% CO2 on the 30th day. The maximal photochemical efficiency of PSⅡ (Fv/Fm) decreased in the first 12 h and then increased with the culture time, and the decline amplitude decreased with the increase of the CO2 concentration, indicating that CO2 slowed down the toxic inhibition of sludge extract to Scenedesmus obliquus, which was expressed as the down-regulation of p53 signaling pathway and protein A0A383WFI7. Proteomic analysis showed that under high-concentration CO2, the protein interaction network with the protein of photosystem II assembly (A0A383VSL5) as the core protein regulated the growth of Scenedesmus obliquus in terms of energy metabolism and material transportation. On the 4th day, Methyltransf_11 domain-containing protein (A0A383VH03) was up-regulated and promoted lipid synthesis, leading to the accumulation of lipids in Scenedesmus obliquus in the early stage and the increase of polysaccharides in the later stage. Collectively, this study revealed the regulation mechanism of CO2 on toxicity removal and carbon distribution of Scenedesmus obliquus.

Biosorption of uranium by immobilized Nostoc sp. and Scenedesmus sp.: kinetic and equilibrium modeling.

Different activities related to uranium mining and nuclear industry may have a negative impact on the environment. Bioremediation of nuclear pollutants using microorganisms is an effective, safe, and economic method. The present study compared the uranium biosorption efficiency of two immobilized algae: Nostoc sp. (cyanophyte) and Scenedesmus sp. (chlorophyte). Effects of metal concentration, contact time, pH, and biosorbent dosage were also studied. The maximum biosorption capacity (60%) by Nostoc sp. was obtained at 300 mg/l uranium solution, 60 min, pH 4.5, and 4.2 g/l algal dosage, whereas Scenedesmus sp. maximally absorbed uranium (65 %) at 150 mg/l uranium solution, 40 min, pH 4.5, and 5.6 g/l of algal dosage. The interaction of metal ions as Na2SO4, FeCl3, CuCl2, NiCl2, CoCl2, CdCl2, and AlCl3 did not support the uranium biosorption by algae. The obtained data was adapted to the linearized form of the Langmuir isotherm model. The experimental qmax values were 130 and 75 mg/g for Nostoc sp. and Scenedesmus sp., respectively. Moreover, the pseudo-second-order kinetic model was more applicable, as the calculated parameters were close to the experimental data. The biosorbents were also characterized by Fourier-transform infrared spectroscopy (ATR-FTIR), energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM) analyses. The results suggest the applicability of algae, in their immobilized form, for recovery and biosorption of uranium from aqueous solution.

Organic onion growth, yield and storage improved by foliar sprays of microalgae and fulvic acid as a natural biofertilizer

The use of microalgae as natural biofertilizer in horticulture has recently been reported, while the use of humic substances is widespread. However, the combined use of microalgae with humic substances applied to plant leaves is still unexploited. Thus, the objective of this work was to combine fulvic acid (FA) with the Scenedesmus subspicatus microalga biomass (SC) as a natural biofertilizer applied via leaf in two onion cultivars in organic system. Four experiments were conducted: i) bioassays to verify the bioactive effect of FA, SC and combinations using the Vigna radiata model plant; ii) greenhouse pot experiment with foliar applications of FA, SC and combination in two onion cultivars; iii) field experiment in organic system with foliar applications of FA, SC and combination in two onion cultivars; and iv) onion bulb storage experiment. The bioactive effect of SC, FA and their combinations was identified by promoting changes in root growth of V. radiata. In pots, treatments containing FA, SC and combination promoted increase in fresh and dry leaf mass. The foliar application of FA, SC and combination promoted an increase in field bulb productivity, reduced mass loss in stored bulbs and increased carbohydrate, amino acid and protein contents in onion bulbs.

The performance of co-immobilized strains isolated from activated sludge combined with Scenedesmus quadricauda to remove nutrients and organics in black odorous water.

In this study, three bacteria were isolated from activated sludge (Pseudomonas aeruginosa, Bacillus subtilis, and Dietzia maris). After that, isolated strains and Scenedesmus quadricauda that could degrade refractory organics, as co-immobilization species, were prepared gel beads to treat black odorous water. Under the optimized conditions, the removal rate of chemical oxygen demand (COD), ammonia nitrogen (NH3-N), total nitrogen (TN) and total phosphorus (TP) reached 94.36%, 95.7%, 91.22% and 95.27%, respectively, and organics (including aromatic proteins and microbial-by-product-like compounds) were also significantly removed. Microbial analysis reveals that the community structure had a significant difference before and after treatment, and the main dominant at the genus level was transformed from Nitrospirillum (approximately 18.03%) to Flavobacterium (approximately 17.64%). This study also found that the immobilized gel beads have excellent stability and reusability, which provided a feasible and robust bioremediation strategy for the treatment of actual black-odor water.