[RAPD analysis of 8 medicinal species of Selaginella].

OBJECTIVE: To identify 8 medicinal species of Selaginella with method of molecular marker and to explore their genetic relationship. METHODS: 8 primers were selected from 60 random primers to amplify the total DNA extracts of 17 samples with RAPD method and then the amplified were analyzed. RESULTS: A total of amplified 58 bands and RAPD map were obtained. Based on them, a Jaccard's genetic similarity matrix and a dendrogram for the 8 plants were established using SPSS 11.5 software. CONCLUSION: The method revealed distinct differences of different species and the variation of the same species of Selaginella from different habitats. It can provide with genetic proof for authentication of some species and units below species.

[Study on HPLC fingerprint characteristics of Selaginella plants].

OBJECTIVE: To establish a method to analyze HPLC fingerprint characteristics of 10 plants from Selaginella. METHOD: HPLC was applied for establishment of fingerprints, which were used to evaluate and distinguish the different species of Selaginella. RESULT: The different species from Selaginella showed different HPLC fingerprint characteristic. The samples of the same species but collected in different period, different environment or different locations showed certain difference in fingerprints CONCLUSION: 2 important mutual fingerprint peaks were found in the 10 plants of Selaginella species and 5 peaks can be used as "main fingerprint peaks". The dates of these peaks can used for assessment of phylogenetic relation among species and evaluation of quality.

[Determination of amentoflavone in 11 species of Selaginella medicinal material by HPLC].

OBJECTIVE: To determine the content of amentoflavone in 11 species of Selaginella medicinal materials. METHODS: HPLC was used with kromasil (250 x 4. 6 mm, 5 microm) column. The mobile phase consisted of methanol and phosphate buffer. The gradient condition was 0-->28 min with methanol 50%-->58%, and 30-->35 min with methanol 58%-->70%. The column temperature was 40 degrees C. The flow rate was 1. 0 ml/min and the detection wavelength at 330 nm. RESULTS: Amentoflavone showed a good linearity in the range of 153 - 767 ng. The average recovery was 97.03% and RSD was 0. 80% (n = 5). CONCLUSION: The method was rapid, accurate and can be used for quality control of Selaginella medicinal materials.

[Investigations and studies on medicinal plants from Selaginellaceae in Hubei Province].

OBJECTIVE: To investigate the species, the distribution and the utilization of the medicinal plants from Selaginellaceae in Hubei Province. METHOD: Through field investigations and comparing the collected specimens and literatures, the classification and identification of the species in Hubei Province were studied. RESULT: The results indicated that 15 species of plants from Selaginellaceae in Hubei, including 14 medicinal and 1 newly recorded species existed. The distribution and use in folk medicine were investigated. And the morphological description of several species was appended. CONCLUSION: The results provided a basis for the exploitation and utilization of the medicinal plant resources of Selagingellaceae.

[Resources of medicinal pteridophyta in Fengyang Mountain and Baishanzu Nature Reservation].

Natural environment of Fengyang Mountain and Baishanzu Nature Reservation is superiority. The vegetation is preserved well and the plant resources is rich. There are 79 species medicinal pteridophyta in this area. According to potency, it can be divided into 16 types, such as medicines for relieving exterior syndrome, medicines for clearing away heat, antirheumatic, medicines for inducing diuresis, medicines for expelling the parasites, hemostasis medicines, medicines for activating blood circulation to remove blood stasis, medicines for restoring vital energy, medicines for calming the liver to stop the wind, etc.