RESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Ephedrae Herba (EH, Ephedra sinica Stapf.) and Armeniacae Semen Amarum (ASA, Prunus armeniaca L. var. ansu Maxim.) have been used to treat asthma, cold, fever, and cough in China for thousands of years. AIM OF THE STUDY: In this study, we aimed to investigate the optimal ratio of EH and ASA compatibility (EAC) to reduce airway injury in asthmatic rats and its possible mechanism. METHODS: Rats were sensitized with a mixture of acetylcholine chloride and histamine bisphosphate 1 h before sensitization by intragastric administration of EAC or dexamethasone or saline for 7 days. Subsequently, the ultrastructure of rat airway epithelial tissue changes, apoptosis of the airway epithelial cells, and the expression of mRNA and protein of EGRF and Bcl-2 were detected. RESULTS: Transmission electron microscope: EAC (groups C and E) had the most prominent effect on repairing airway epithelial cells' ultrastructural changes in asthmatic rats. TUNEL: dexamethasone and EAC (groups BãCãE and F) inhibited the apoptosis of airway epithelial cells in asthmatic rats (P < 0.05). In situ hybridization: EAC (group E) inhibited the overexpression of EGFR and Bcl-2 mRNA (P < 0.05).Western Blotting: EAC (groups AãBãCãE and F) inhibited the upregulation of airway epithelial EGFR and Bcl-2 protein expression (P < 0.01). CONCLUSIONS: Our findings indicate that EAC can inhibit abnormal changes in airway epithelial structure and apoptosis of airway epithelial cells, thereby alleviating airway injury. In this study, the best combination of EH and ASA to alleviate airway epithelial injury in asthmatic rats was group E (EH: ASA = 8: 4.5).
Asunto(s)
Asma/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Ephedra sinica/química , Prunus armeniaca/química , Sistema Respiratorio/efectos de los fármacos , Acetilcolina/toxicidad , Animales , Apoptosis/efectos de los fármacos , Asma/inducido químicamente , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/uso terapéutico , Células Epiteliales/efectos de los fármacos , Células Epiteliales/patología , Células Epiteliales/ultraestructura , Receptores ErbB/antagonistas & inhibidores , Receptores ErbB/biosíntesis , Receptores ErbB/genética , Histamina/análogos & derivados , Histamina/toxicidad , Masculino , Proteínas Proto-Oncogénicas c-bcl-2/antagonistas & inhibidores , Proteínas Proto-Oncogénicas c-bcl-2/biosíntesis , Proteínas Proto-Oncogénicas c-bcl-2/genética , Ratas Sprague-Dawley , Sistema Respiratorio/lesiones , Sistema Respiratorio/patología , Sistema Respiratorio/ultraestructura , Tráquea/efectos de los fármacos , Tráquea/lesiones , Tráquea/patología , Tráquea/ultraestructuraRESUMEN
A characteristic feature of bromium acute intoxications in levomycetine production is the delayed hemorrhagic component manifestation in the clinical course of toxic bronchitis (on the 20th day after inhaling bromium vapours), and the changes in the heart in the form of myocardial toxic dystrophia, as well as the growing activity of some liver related enzymes, which is indicative of the hepatocyte membrane lesions.
Asunto(s)
Accidentes de Trabajo , Bromo/envenenamiento , Quemaduras Químicas/complicaciones , Quemaduras por Inhalación/complicaciones , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Sistema Respiratorio/lesiones , Tecnología Farmacéutica/normas , Enfermedad Aguda , Adulto , Humanos , Masculino , Sistema Respiratorio/efectos de los fármacos , VolatilizaciónRESUMEN
We present the fingings in a patient with acute thermal burn to the upper and lower airway who developed mucosal edema followed by patchy areas of granuloma-like lesions in the trachea and bronchi. A four-month follow-up showed resolution of the gross lesions, but functional alterations remained. This patient illustrates the necessity for repeated direct observation and functional evaluation of the lower airway folllowing thermal injury, which can be a life-threatening disorder.