RESUMEN
The primo vascular system (PVS) is being established as a circulatory system that corresponds to acupuncture meridians. There have been two critical questions in making the PVS accepted as a novel liquid flowing system. The first one was directly to show the flow of liquid in PVS and the second one was to explain why it was not observed in the conventional histological study of animal tissues. Flow in the PVS in the abdominal cavity was previously verified by injecting Alcian blue into a primo node. However, the tracing of the dye to other subsystems of the PVS has not been done. In the current work we injected fluorescent nanoparticles (FNPs) into a primo node and traced them along a primo vessel which was inside a fat tissue in the abdominal wall. Linea alba is a white middle line in the abdominal skin of a mammal and a band of fat tissue is located in parallel to the linea alba in the parietal side of the abdominal wall of a rat. In this fat band a primo vessel runs parallel to the prominent blood vessels in the fat band and is located just inside the parietal peritoneum. About the second question on the reason why the PVS was not in conventional histological study the current work provided the answer. Histological analysis with hematoxyline and eosine, Masson's trichrome, and Toluidine blue could not discriminate the primo vessel even when we knew the location of the PVS by the trace of the FNPs. This clearly explains why the PVS is hard to observe in conventional histology: it is not a matter of resolution but the contrast. The PVS has very similar structure to the connective tissues that surround the PVS. In the current work we propose a method to find the PVS: Observation of mast cell distribution with toluidine blue staining and the PN has a high density of mast cells, while the lymph node has low density.
Asunto(s)
Cavidad Abdominal/anatomía & histología , Grasa Abdominal/anatomía & histología , Pared Abdominal/anatomía & histología , Puntos de Acupuntura , Nanopartículas/química , Coloración y Etiquetado/métodos , Cavidad Abdominal/irrigación sanguínea , Grasa Abdominal/irrigación sanguínea , Grasa Abdominal/citología , Pared Abdominal/irrigación sanguínea , Azul Alcián/química , Animales , Colorantes/química , Eosina Amarillenta-(YS) , Hematoxilina , Humanos , Ganglios Linfáticos/irrigación sanguínea , Ganglios Linfáticos/citología , Vasos Linfáticos/anatomía & histología , Vasos Linfáticos/irrigación sanguínea , Masculino , Mastocitos/citología , Ratas , Ratas Sprague-Dawley , Reología , Rodaminas/química , Cloruro de Tolonio/químicaRESUMEN
We report a case of a 44-year-old female patient, presented to us after years of recurrent intermittent episodes of unilateral left neck swelling. An MR lymphangiogram demonstrated a lymphatic varix at the confluence of the left upper extremity lymphatic ducts, confirmed by intranodal axillary lymphangiography. After successful catheterization of the feeding lymphatic vessels, the varix was successfully embolized with detachable microcoils and an autologous blood patch. The patient has been free from symptoms on subsequent outpatient follow-up.