RESUMEN
Fumonisin B1 (FB1) is the most common food-borne mycotoxin produced by the Fusarium species, posing a potential threat to human and animal health. Pigs are more sensitive to FB1 ingested from feed compared to other farmed livestock. Enzymatic degradation is an ideal detoxification method that has attracted much attention. This study aimed to explore the functional characteristics of the carboxylesterase FumDSB in growing pigs from the perspective of brain-gut regulation. A total of 24 growing pigs were divided into three groups. The control group was fed a basal diet, the FB1 group was supplemented with FB1 at 5 mg/kg feed, and the FumDSB group received added FumDSB based on the diet of the FB1 group. After 35 days of animal trials, samples from the hypothalamus and jejunum were analyzed through HE staining, qRT-PCR and immunohistochemistry. The results demonstrated that the ingestion of FB1 can reduce the feed intake and weight gain of growing pigs, indicating that several appetite-related brain-gut peptides (including NPY, PYY, ghrelin and obestatin, etc.) play important roles in the anorexia response induced by FB1. After adding FumDSB as detoxifying enzymes, however, the anorexia effects of FB1 were alleviated, and the expression and distribution of the corresponding brain-gut peptides exhibited a certain degree of regulation. In conclusion, the addition of FumDSB can reduce the anorexia effects of FB1 by regulating several brain-gut peptides in both the hypothalamus and the jejunum of growing pigs.
Asunto(s)
Carboxilesterasa/metabolismo , Fumonisinas/metabolismo , Fumonisinas/toxicidad , Crecimiento y Desarrollo/efectos de los fármacos , Hipotálamo/efectos de los fármacos , Yeyuno/efectos de los fármacos , Proteolisis/efectos de los fármacos , Porcinos/crecimiento & desarrollo , Animales , Hipotálamo/metabolismo , Yeyuno/metabolismo , Venenos/metabolismo , Venenos/toxicidadRESUMEN
Aconitine (AC) is well-known as the main toxic ingredient and active compound of Aconitum species, of which several aconites are essential herbal medicines of Traditional Chinese Medicine (TCM) and widely applied to treat diverse diseases for their excellent anti-inflammatory, analgesic, and cardiotonic effects. However, the cardiotoxicity and neurotoxicity of AC attracted a lot of attention and made it a favorite botanic poison in history. Nowadays, the narrow therapeutic window of AC limits the clinical application of AC-containing herbal medicines; overdosing on AC always induces ventricular tachyarrhythmia and heart arrest, both of which are potentially lethal. But the underlying cardiotoxic mechanisms remained chaos. Recently, beyond its cardiotoxic effects, emerging evidence shows that low doses of AC or its metabolites could generate cardioprotective effects and are necessary to aconite's clinical efficacy. Consistent with TCM's theory that even toxic substances are powerful medicines, AC thus could not be simply identified as a toxicant or a drug. To prevent cardiotoxicity while digging the unique value of AC in cardiac pharmacology, there exists a huge urge to better know the characteristic of AC being a cardiotoxic agent or a potential heart drug. Here, this article reviews the advances of AC metabolism and focuses on the latest mechanistic findings of cardiac efficacy and toxicity of this aconite alkaloid or its metabolites. We also discuss how to prevent AC-related cardiotoxicity, as well as the issues before the development of AC-based medicines that should be solved, to provide new insight into the paradoxical nature of this ancient poison.
Asunto(s)
Aconitum , Medicamentos Herbarios Chinos , Venenos , Aconitina/efectos adversos , Aconitina/toxicidad , Medicamentos Herbarios Chinos/efectos adversos , Humanos , Venenos/toxicidadRESUMEN
Increased intestinal permeability has been implicated in various pathologies, has various causes, and can develop during vigorous athletic training. Colostrum bovinum is a natural supplement with a wide range of supposed positive health effects, including reduction of intestine permeability. We assessed influence of colostrum supplementation on intestinal permeability related parameters in a group of 16 athletes during peak training for competition. This double-blind placebo-controlled study compared supplementation for 20 days with 500 mg of colostrum bovinum or placebo (whey). Gut permeability status was assayed by differential absorption of lactulose and mannitol (L/M test) and stool zonulin concentration. Baseline L/M tests found that six of the participants (75%) in the colostrum group had increased intestinal permeability. After supplementation, the test values were within the normal range and were significantly lower than at baseline. The colostrum group Δ values produced by comparing the post-intervention and baseline results were also significantly lower than the placebo group Δ values. The differences in stool zonulin concentration were smaller than those in the L/M test, but were significant when the Δ values due to intervention were compared between the colostrum group and the placebo group. Colostrum bovinum supplementation was safe and effective in decreasing of intestinal permeability in this series of athletes at increased risk of its elevation.
Asunto(s)
Productos Biológicos/uso terapéutico , Calostro/química , Suplementos Dietéticos , Fármacos Gastrointestinales/uso terapéutico , Enfermedades Gastrointestinales/prevención & control , Mucosa Intestinal/metabolismo , Estrés Fisiológico , Adulto , Animales , Atletas , Productos Biológicos/efectos adversos , Bovinos , Permeabilidad de la Membrana Celular/efectos de los fármacos , Toxina del Cólera/análisis , Toxina del Cólera/antagonistas & inhibidores , Toxina del Cólera/metabolismo , Toxina del Cólera/toxicidad , Estudios Cruzados , Suplementos Dietéticos/efectos adversos , Método Doble Ciego , Heces/química , Liofilización , Fármacos Gastrointestinales/efectos adversos , Enfermedades Gastrointestinales/inducido químicamente , Enfermedades Gastrointestinales/metabolismo , Enfermedades Gastrointestinales/fisiopatología , Haptoglobinas , Humanos , Absorción Intestinal/efectos de los fármacos , Mucosa Intestinal/efectos de los fármacos , Mucosa Intestinal/fisiología , Mucosa Intestinal/fisiopatología , Masculino , Artes Marciales , Venenos/análisis , Venenos/química , Venenos/metabolismo , Venenos/toxicidad , Polonia , Precursores de Proteínas , ToxicocinéticaRESUMEN
The aim of this study was to develop the first CE-based method enabling separation of 20 structurally similar coumarin derivatives. To facilitate method optimization a series of three consequent Doehlert experimental designs with the response surface methodology was employed, using number of peaks and the adjusted time of analysis as the selected responses. Initially, three variables were examined: buffer pH, ionic strength and temperature (No. 1 Doehlert design). The optimal conditions provided only partial separation, on that account, several buffer additives were examined at the next step: organic cosolvents and cyclodextrin (No. 2 Doehlert design). The optimal cyclodextrin type was also selected experimentally. The most promising results were obtained for the buffers fortified with methanol, acetonitrile and heptakis(2,3,6-tri-O-methyl)-ß-cyclodextrin. Since these additives may potentially affect acid-base equilibrium and ionization state of analytes, the third Doehlert design (No. 3) was used to reconcile concentration of these additives with optimal pH. Ultimately, the total separation of all 20 compounds was achieved using the borate buffer at basic pH 9.5 in the presence of 10mM cyclodextrin, 9% (v/v) acetonitrile and 36% (v/v) methanol. Identity of all compounds was confirmed using the in-lab build UV-VIS spectra library. The developed method succeeded in identification of coumarin derivatives in three real samples. It demonstrates a huge resolving power of CE assisted by addition of cyclodextrins and organic cosolvents. Our unique optimization approach, based on the three Doehlert designs, seems to be prospective for future applications of this technique.
Asunto(s)
Manzanilla/química , Cumarinas/aislamiento & purificación , Electroforesis Capilar/métodos , Extractos Vegetales/química , Venenos/química , Proyectos de Investigación/normas , Acetonitrilos/química , Animales , Cumarinas/análisis , Concentración de Iones de Hidrógeno , Metanol/química , Concentración Osmolar , Venenos/toxicidad , Ratas , Temperatura , beta-Ciclodextrinas/químicaRESUMEN
Aflatoxin B1 (AFB1) has strong carcinogenicity. Consumption of AFB1-contaminated agricultural products and the occurrence of hepatocellular carcinoma have received widespread attention. The aim of this paper was to investigate whether zinc supplementation could inhibit AFB1-induced cytotoxicity and genotoxicity in HepG2 cells and the mechanism of this inhibition. Our data suggest that zinc sources can relieve a certain degree of AFB1-induced cytotoxicity and genotoxicity by protecting against apoptotic body formation and DNA strand breaks, affecting S phase cell cycle arrest, reducing 8-OHdG formation, inhibiting global DNA hypomethylation and regulating gene expression in antioxidation, zinc-association and apoptosis processes. Consequently, zinc stabilizes the integrity of DNA and improves cell survival. These data provides new insights into the protective role of zinc in alleviating AFB1-induced cytotoxicity and mediating epigenetic changes in hepatocytes, demonstrating that zinc sources have detoxification properties in mycotoxin-induced toxicity.
Asunto(s)
Aflatoxina B1/toxicidad , Supervivencia Celular/efectos de los fármacos , Daño del ADN/efectos de los fármacos , Metilación de ADN/efectos de los fármacos , Hepatocitos/efectos de los fármacos , Venenos/toxicidad , Zinc/farmacología , Antioxidantes/metabolismo , Ciclo Celular/efectos de los fármacos , Células Hep G2 , Hepatocitos/patología , Humanos , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Oligoelementos/farmacologíaRESUMEN
To evaluate the renal toxicity of dietary aflatoxin B1 (AFB1) and ameliorating effects of added dietary sodium selenite in broiler, renal histopathological changes, ultrastructural changes, and renal function parameters were monitored at 7, 14, and 21 days of age. Two hundred one-day-old healthy male Avian broilers were divided into four groups, namely control group, AFB1 group (0.3 mg/kg AFB1), +Se group (0.4 mg/kg Se), and AFB1+Se group (0.3 mg/kg AFB1+0.4 mg/kg Se). Compared with that of the control group, the relative weight of kidney was increased in the AFB1 group. There were no significant differences between the AFB1+Se group and the control group. By histopathological observation, the renal epithelia were swelling and necrosis at 7 and 21 days of age. Ultrastructurally, the lipid droplets and expanded endoplasmic reticulum appeared in the plasma of epithelia cells in the AFB1 group. Enlarged mitochondria with degenerated cristae were observed in the +Se group. Compared with the control group, the contents of serum creatinine and serum uric acid in the AFB1 group were increased, while the activity of renal Naâº-K⺠ATPase was decreased. When 0.4 mg/kg selenium was added into the diet containing 0.3 mg/kg AFB1, there were no obvious histological changes in the AFB1+Se group, and the contents of the serum creatinine and serum uric acid contents and the activity of renal Naâº-K⺠ATPase were close to those in the control group. In conclusion, sodium selenite exhibited protective effects on AFB1-induced kidney toxicity in broilers.
Asunto(s)
Aflatoxina B1/toxicidad , Enfermedades Renales/prevención & control , Riñón/efectos de los fármacos , Venenos/toxicidad , Selenito de Sodio/farmacología , Oligoelementos/farmacología , Animales , Peso Corporal , Pollos , Creatinina/sangre , Suplementos Dietéticos , Riñón/ultraestructura , Enfermedades Renales/sangre , Enfermedades Renales/patología , Masculino , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Ácido Úrico/sangreRESUMEN
Pigmented rice bran has been suggested to be a valuable source of beneficial phytochemicals. We investigated genotoxic and anti-genotoxic effects of purple rice bran extract (PRBE) in rats using a liver micronucleus assay. Purple rice bran was extracted with methanol, obtaining large amounts of phenolic compounds, including anthocyanins and small amounts of gamma-oryzanol. The experimental protocols were divided into two sets. Male rats were divided into three groups. Group 1 was a negative control, while Groups 2 and 3 were fed with 100 and 500 mg/kg bw of PRBE, respectively, for 28 days. PRBE had no effect on micronucleus formation or xenobiotic metabolizing enzymes in rat liver. Experiments concerning the effect of PRBE on AFB1 showed that PRBE significantly lessened the amount of micronucleated hepatocytes in AFB1 treated rats. Furthermore, it modulated metabolic activation of AFB1 metabolism in the liver by suppressing activity and protein expression of CYP1A2, CYP3A and CYP 450 reductase, and enhancing phase II enzymes including GST and UGT. Overall, purple rice bran extract was not genotoxic in rats. It exhibited anti-genotoxicity by modulation some xenobiotic enzymes active in AFB1 metabolism.
Asunto(s)
Aflatoxina B1/toxicidad , Hepatocitos/efectos de los fármacos , Hígado/efectos de los fármacos , Micronúcleos con Defecto Cromosómico/efectos de los fármacos , Oryza , Extractos Vegetales/farmacología , Venenos/toxicidad , Animales , Carcinogénesis/efectos de los fármacos , Citocromo P-450 CYP1A2 , Citocromo P-450 CYP3A/efectos de los fármacos , Citocromo P-450 CYP3A/metabolismo , Citocromos/efectos de los fármacos , Citocromos/metabolismo , Glucuronosiltransferasa/efectos de los fármacos , Glucuronosiltransferasa/metabolismo , Glutatión Transferasa/efectos de los fármacos , Glutatión Transferasa/metabolismo , Neoplasias Hepáticas Experimentales , Masculino , Micronúcleos con Defecto Cromosómico/inducido químicamente , Pruebas de Micronúcleos , NADPH-Ferrihemoproteína Reductasa , RatasRESUMEN
Aflatoxin B1 (AFB1) is a mycotoxin that causes cytotoxicity through oxidative damage to its target organs. The liver is the first target of AFB1 damage. The aim of this study was to evaluate the protective effect of selenium on AFB1-induced hepatic mitochondrial damage in ducklings using molecular biological and histopathological techniques. Aflatoxin was administered via intragastric intubation (0.1 mg/kg body weight), daily for 21 days. The experimental group also received intragastric sodium selenite (1 mg/kg body weight), while the control group was given the same volume of dimethyl sulfoxide (DMSO). Sequence analysis of the mitochondrial DNA D-loop region showed that AFB1 induced damage. All AFB1-administrated ducklings were identified as having D-loop mitochondrial DNA mutations. Mutations were detected in two ducklings that had received both AFB1 and selenium. Mitochondrial swelling assays showed that opening of the mitochondrial permeability transition pores was increased in ducklings that had received AFB1 for 14 and 21 days (P < 0.05). Selenium significantly attenuated these adverse effects of AFB1. After AFB1 exposure, histological alterations were observed, including fat necrosis, steatosis, and formation of lymphoid nodules with infiltrated lymphocytes. These histological abnormalities were also attenuated by treatment with selenium. The overall data indicated that selenium exerts a potent protective effect against AFB1-induced hepatic mitochondrial damage, possibly through its antioxidant activity.
Asunto(s)
Aflatoxina B1/toxicidad , Daño del ADN , Mitocondrias Hepáticas/metabolismo , Membranas Mitocondriales/metabolismo , Venenos/toxicidad , Selenio/farmacología , Animales , Patos , Mitocondrias Hepáticas/patología , Membranas Mitocondriales/patología , PermeabilidadRESUMEN
BACKGROUND: Toxin-induced methemoglobinemia is seen in poisoning with oxidizing agents. We report the clinical features and outcome of patients admitted with severe methemoglobinemia due to intentional ingestion of toxicants. METHODS: In this observational case series, patients admitted with toxin-induced methemoglobinemia between September 2011 and January 2014 were identified from the institutional poisoning database. Clinical profile and outcome of patients with methemoglobin concentration greater than or equal to 49% is reported. RESULTS: Of the 824 patients admitted with poisoning, 5 patients with methemoglobin concentration greater than or equal to 49% were included. The implicated compounds were nitrobenzene, benzoylphenylurea, flubendamide and Rishab(TM). One patient refused to name the compound. All patients were managed in the intensive care unit. Altered sensorium [Glasgow coma scale (GCS) < 10] was common (80%); 2 patients presented with a GCS greater than 4. All patients manifested cyanosis, low oxygen saturation and chocolate-brown-colored blood despite supplemental oxygen therapy. The median methemoglobin concentration was 64.7% (range 49.8-91.6%); 2 patients had methemoglobin concentration greater than 70%. One patient needed inotropes. Four patients required mechanical ventilation for 4-14 days. All patients were treated with methylene blue; 4 received more than one dose. Three patients also received intravenous ascorbic acid 500 mg, once daily, for 3 days. Following treatment, there was evidence of haemolysis in all patients; 2 required blood transfusion. All patients survived. CONCLUSION: Patients with severe toxin-induced methemoglobinemia present with altered sensorium and cyanosis and may require ventilatory support and inotropes. Though methemoglobin concentrations greater than 70% are considered fatal, aggressive management with methylene blue and supportive therapy can lead to survival.
Asunto(s)
Metahemoglobinemia/diagnóstico , Venenos/toxicidad , Administración Oral , Adulto , Cianosis/inducido químicamente , Cianosis/diagnóstico , Cianosis/tratamiento farmacológico , Humanos , Metahemoglobina/metabolismo , Metahemoglobinemia/inducido químicamente , Metahemoglobinemia/tratamiento farmacológico , Azul de Metileno/uso terapéutico , Persona de Mediana Edad , Nitrobencenos/toxicidad , Adulto JovenRESUMEN
Poisons have long been used to kill wildlife throughout the world. An evolution has occurred from the use of plant- and animal-based toxins to synthetic pesticides to kill wildlife, a method that is silent, cheap, easy, and effective. The use of pesticides to poison wildlife began in southern Africa, and predator populations were widely targeted and eliminated. A steep increase has recently been observed in the intensity of wildlife poisonings, with corresponding population declines. However, the majority of poisonings go unreported. Under national laws, it is illegal to hunt wildlife using poisons in 83% of African countries. Pesticide regulations are inadequate, and enforcement of existing legislation is poor. Few countries have forensic field protocols, and most lack storage and testing facilities. Methods used to poison wildlife include baiting carcasses, soaking grains in pesticide solution, mixing pesticides to form salt licks, and tainting waterholes. Carbofuran is the most widely abused pesticide in Africa. Common reasons for poisoning are control of damage-causing animals, harvesting fish and bushmeat, harvesting animals for traditional medicine, poaching for wildlife products, and killing wildlife sentinels (e.g., vultures because their aerial circling alerts authorities to poachers' activities). Populations of scavengers, particularly vultures, have been decimated by poisoning. Recommendations include banning pesticides, improving pesticide regulations and controlling distribution, better enforcement and stiffer penalties for offenders, increasing international support and awareness, and developing regional pesticide centers.
Asunto(s)
Animales Salvajes/fisiología , Plaguicidas/envenenamiento , Venenos/toxicidad , África , Animales , Conservación de los Recursos Naturales , Historia del Siglo XX , Venenos/historiaRESUMEN
Poisoning by cyanide can be verified by analysis of the cyanide detoxification product, α-ketoglutarate cyanohydrin (α-KgCN), which is produced from the reaction of cyanide and endogenous α-ketoglutarate. Although α-KgCN can potentially be used to verify cyanide exposure, limited toxicokinetic data in cyanide-poisoned animals are available. We, therefore, studied the toxicokinetics of α-KgCN and compared its behavior to other cyanide metabolites, thiocyanate and 2-amino-2-thiazoline-4-carboxylic acid (ATCA), in the plasma of 31 Yorkshire pigs that received KCN (4mg/mL) intravenously (IV) (0.17 mg/kg/min). α-KgCN concentrations rose rapidly during KCN administration until the onset of apnea, and then decreased over time in all groups with a half-life of 15 min. The maximum concentrations of α-KgCN and cyanide were 2.35 and 30.18 µM, respectively, suggesting that only a small fraction of the administered cyanide is converted to α-KgCN. Although this is the case, the α-KgCN concentration increased >100-fold over endogenous concentrations compared to only a three-fold increase for cyanide and ATCA. The plasma profile of α-KgCN was similar to that of cyanide, ATCA, and thiocyanate. The results of this study suggest that the use of α-KgCN as a biomarker for cyanide exposure is best suited immediately following exposure for instances of acute, high-dose cyanide poisoning.
Asunto(s)
Ácidos Cetoglutáricos/farmacocinética , Ácidos Cetoglutáricos/toxicidad , Nitrilos/farmacocinética , Nitrilos/toxicidad , Venenos/toxicidad , Cianuro de Potasio/toxicidad , Animales , Arginina/sangre , Cianuros/sangre , Semivida , Indicadores y Reactivos , Infusiones Intravenosas , Farmacocinética , Porcinos , Tiazolidinas/sangre , Tiocianatos/sangreRESUMEN
A total of 1,280 1-d-old ducks were used in a study to investigate the effects of increasing aflatoxin B1 (AFB1) concentrations from naturally contaminated corn on young ducklings, and the effectiveness of a clay adsorbent (CA) to protect against those effects. Ducks were randomly allotted to 8 treatments (TRT) in a 4 × 2 factorial arrangement with 4 levels of AFB1 (0, 25, 50, and 100 µg/kg) and 2 levels of CA (0 and 0.1%) with 8 pens per TRT and 20 ducks per pen. All ducks were allowed ad libitum access to feed and water during the 21-d experiment. The ADG, ADFI, feed conversion rate, mortality, bill color, and CV of BW of each replicate were measured at the end of the study. Blood and tissue samples from 8 ducks per TRT were obtained on d 21 of the experiment to determine the serum immunoglobulin and protein concentrations, relative organ weights, and intestinal morphology. Average daily gain and relative weights of the liver, spleen, thymus, and bursa of Fabricius decreased linearly (P < 0.05) as dietary AFB1 increased. Serum proteins and intestinal villi heights and villus/crypt ratio followed the same pattern. Bill decolorization ratio, CV of BW, and mortality increased linearly (P < 0.05) as dietary AFB1 increased. Adding 0.1% CA to the diet improved (P < 0.05) the relative weights of the small intestine, spleen, and thymus, and the villus height and villus/crypt ratio of the duodenum and jejunum, as well as the serum IgG and IgM concentrations. Adding CA also reduced (P < 0.05) bill decolorization ratio, CV of BW, mortality, and serum IgA concentration. Therefore, duck performance was negatively affected by increasing AFB1 concentrations in diets. But the addition of 0.1% CA can protect against the detrimental effects caused by AFB1-contaminated corn in diets for ducks.
Asunto(s)
Aflatoxina B1/toxicidad , Silicatos de Aluminio/administración & dosificación , Alimentación Animal/microbiología , Patos/metabolismo , Microbiología de Alimentos , Venenos/toxicidad , Adsorción , Animales , Análisis Químico de la Sangre/veterinaria , Cromatografía Líquida de Alta Presión/veterinaria , Arcilla , Dieta/veterinaria , Suplementos Dietéticos , Patos/crecimiento & desarrollo , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Fluorometría/veterinaria , Intestinos/microbiología , Intestinos/patología , Masculino , Zea mays/microbiologíaAsunto(s)
Hipoglucemiantes/administración & dosificación , Insulina/administración & dosificación , Venenos/toxicidad , Choque Cardiogénico/tratamiento farmacológico , Antagonistas Adrenérgicos beta/envenenamiento , Animales , Bloqueadores de los Canales de Calcio/envenenamiento , Humanos , Hipoglucemiantes/uso terapéutico , Insulina/uso terapéutico , Choque Cardiogénico/inducido químicamente , Bloqueadores de los Canales de Sodio/envenenamientoRESUMEN
Aflatoxicosis is a cause of economic losses in broiler production. In this study, the effect of one commercial nanocompound, Nanocid (Nano Nasb Pars Co., Iran) was evaluated in reduction of aflatoxin effects on the growth and performance indices in broiler chickens suffering from experimental aflatoxicosis. For this, a total of 300 one-day-old broiler chicks (Ross strain) were randomly divided into 4 groups with 3 replicates of 15 chicks in each separated pen during the 28-day experiment. Treatment groups including group A: chickens fed basal diet, group B: chickens fed 3 ppm productive aflatoxin in basal diet, group C: chickens fed basal diet plus 2500 ppm Nanocid, and group D: chickens fed 3 ppm productive aflatoxin and 2500 ppm Nanocid, in basal diet. Data on body weight, body weight gain (BWG), feed intake, and feed conversion ratio (FCR) were recorded at weekly intervals. Also cumulative data were assessed. Results showed, although supplement of Nanocid to conventional diet had no effect on performance but addition of Nanocid to diet containing 3 ppm aflatoxin increased significantly the cumulative BWG, cumulative feed consumption and decreased FCR in the last 2 weeks of experimental period. The improvement in these performance indices by supplement of Nanocid to diet containing aflatoxin showed the ability of Nanocid to diminish the inhibitory effects of aflatoxin.
Asunto(s)
Aflatoxinas/toxicidad , Pollos/crecimiento & desarrollo , Nanopartículas del Metal/administración & dosificación , Micotoxicosis/tratamiento farmacológico , Venenos/toxicidad , Enfermedades de las Aves de Corral/tratamiento farmacológico , Compuestos de Plata/farmacología , Animales , Dieta , Modelos Animales de Enfermedad , Ingestión de Alimentos/efectos de los fármacos , Contaminación de Alimentos , Micotoxicosis/etiología , Micotoxicosis/fisiopatología , Enfermedades de las Aves de Corral/inducido químicamente , Enfermedades de las Aves de Corral/fisiopatología , Aumento de Peso/efectos de los fármacosRESUMEN
Microelectrode array (MEA) approaches have been proposed as a tool for detecting functional changes in electrically excitable cells, including neurons, exposed to drugs, chemicals or particles. However, conventional single well-MEA systems lack the throughput necessary for screening large numbers of uncharacterized compounds. Recently, multi-well MEA (mwMEA) formats have become available to address the need for increased throughput. The current experiments examined the effects of a training set of 30 chemicals on spontaneous activity in networks of cortical neurons grown on mwMEA plates. Each plate contained 12 wells with 64 microelectrodes/well, for a total of 768 channels. Of the 30 chemicals evaluated, 23 were known to alter neuronal function in vivo ("positives"), including 6 GABAergic and 3 glutamatergic antagonists/agonists, 4 pyrethroids, 3 metals, 2 cholinesterase inhibitors, 2 nicotinic acetylcholine receptor agonists, valproic acid, verapamil, and fluoxetine. Seven compounds expected to have no effect on neuronal function were tested as "negatives" (glyphosate, acetaminophen, salicylic acid, paraquat, saccharin, d-sorbitol and amoxicillin). Following collection of 33 min of baseline activity, chemical effects (50 µM or highest soluble concentration) were recorded for 33 min. Twenty of the positives altered the mean network spike rate by more than the 14% threshold (two standard deviations from the mean for DMSO control). The three positives without effect were bifenthrin, nicotine and imidacloprid. None of the negative compounds caused a change in activity beyond the threshold. Based on these results, the mwMEA assay has both high sensitivity (87% identification of positive compounds) and specificity (100% identification of negative compounds). These experiments demonstrate the capacity of mwMEAs to screen compounds for neurotoxic effects mediated by a broad variety of mechanisms.
Asunto(s)
Evaluación Preclínica de Medicamentos/instrumentación , Microelectrodos , Red Nerviosa/efectos de los fármacos , Neuronas/efectos de los fármacos , Venenos/toxicidad , Pruebas de Toxicidad/instrumentación , Potenciales de Acción/efectos de los fármacos , Animales , Animales Recién Nacidos , Células Cultivadas , Corteza Cerebral/citología , Relación Dosis-Respuesta a Droga , Neuronas/fisiología , Neurotransmisores/toxicidad , Ratas , Ratas Long-Evans , Tetrodotoxina/toxicidadRESUMEN
Spirulina (SPN) and Whey protein (WPC) are being touted as functional foods with a number of health benefits. SPN is blue green algae while WPC is a protein complex derived from milk and both have strong antioxidant activity and provoke a free radical scavenging enzyme system. The aim of the present study was to evaluate the antioxidant potentials of SPN and WPC to regulate the alteration of genes' expression and counteract oxidative stress in rats during aflatoxecosis. Eighty male Sprague-Dawley rats were divided into eight groups, which included the control group, the group fed with aflatoxins (AFs)-contaminated diet (2.5 mg/kg diet) for 30 day, the group treated orally with WPC (300 mg/kg b.w.), the group treated orally with SPN (50 mg/kg b.w), the group treated orally with WPC plus SPN and the groups fed with AFs-contaminated diet and treated orally with WPC, SPN and/or WPC. Oxidative stress markers and gene expression were assayed in liver and testis and the damage of DNA was evaluated by DNA fragmentation and micronucleus tests. The results demonstrated that supplementation of SPN and/or WPC reduced the oxidative stress induced by AFs as indicated by decreased lipid peroxidation level, increased glutathione content and up-regulated PHGPx gene expression. Both agents succeed to inhibit DNA damage as indicated by the down-regulation of Fas gene expression, and decreased the percentage of DNA fragmentation and micronucleated erythrocytes. Moreover, WPC was found to be effective than SPN and the combined treatment was more effective than the single treatment. It could be concluded that both SPN and WPC induced a protective action and regulated the alteration of genes expression induced by AFs; however, the combined treatment may be useful than the single treatment.
Asunto(s)
Antioxidantes/farmacología , Suplementos Dietéticos , Regulación de la Expresión Génica , Proteínas de la Leche/farmacología , Spirulina , Aflatoxinas/toxicidad , Animales , Daño del ADN , Regulación hacia Abajo/efectos de los fármacos , Eritrocitos/metabolismo , Expresión Génica/efectos de los fármacos , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Hígado/metabolismo , Masculino , Estrés Oxidativo/efectos de los fármacos , Venenos/toxicidad , Ratas , Ratas Sprague-Dawley , Testículo/metabolismo , Proteína de Suero de LecheRESUMEN
The present study was aimed to test the hypothesis that inorganic phosphate may reduce arsenic toxicity by decreasing its intestinal transference. Co-administration of inorganic phosphate (6.56 M) and arsenic (6.07 mM) in the intestinal loops of rats, in situ, caused significant reduction of arsenic transference. Short-term arsenic exposure (3mg/kg body weight/day for 30 days) caused liver damage evidenced by activities of liver enzymes and necroinflammatory changes. These effects of arsenic were coupled with enhanced mitochondrial swelling, inhibition of cytochrome c oxidase, Ca(2+)-ATPase, a decrease in mitochondrial calcium content, changes in indices of hepatic mitochondrial oxidative stress and iNOS expression. Arsenic also increased hepatic caspase 3 activity and DNA fragmentation. All these apoptosis-related molecular changes caused by arsenic could be alleviated by supplementation with inorganic phosphate, which likely suggests a protective role of phosphate against arsenic-induced hepatotoxic changes.
Asunto(s)
Arsénico/antagonistas & inhibidores , Arsénico/toxicidad , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Mitocondrias Hepáticas/efectos de los fármacos , Mitocondrias Hepáticas/patología , Fosfatos/uso terapéutico , Fósforo Dietético/farmacología , Venenos/toxicidad , Animales , Intoxicación por Arsénico/metabolismo , Caspasa 3/metabolismo , Catalasa/metabolismo , Fragmentación del ADN/efectos de los fármacos , Suplementos Dietéticos , Radical Hidroxilo/metabolismo , Mucosa Intestinal/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Hígado/patología , Masculino , Membranas Mitocondriales/efectos de los fármacos , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismoRESUMEN
The purpose of this study was evaluation of ethanolic turmeric extract (ETE; Curcuma longa) effect on overall performance including body weight (BW), body weight gain (BWG), feed intake and feed conversion ratio (FCR) weekly and cumulative for a period of 4 weeks with 300 commercial broiler chicks (Ross strain). These chicks were randomly divided into four groups with three replicates of 15 chicks in each replicate. In group A, chickens were fed a basal diet, in group B, chickens were fed a basal diet plus 3 ppm productive aflatoxin. In group C, chickens consumed a basal diet plus 0.05% ETE and in group D, chickens received a basal diet with 0.05% ETE plus 3 ppm productive aflatoxin. Aflatoxin production by Aspergillus parasiticus (PTTC NO:1850) in maize was according to the Shotwell method. The results revealed that there were no significant differences in BW, BWG and FCR between groups fed turmeric at 0.05% and the control group. The supplement of ETE in a diet containing 3 ppm aflatoxin can significantly improve performance indices compared with the group that consumed aflatoxin alone. In conclusion, our results suggest that turmeric extract (Curcuma longa) can provide protection against the negative effects of aflatoxin on performance of broiler chickens.
Asunto(s)
Antioxidantes/administración & dosificación , Pollos , Curcuma/química , Micotoxicosis/dietoterapia , Extractos Vegetales/administración & dosificación , Enfermedades de las Aves de Corral/tratamiento farmacológico , Aflatoxinas/toxicidad , Alimentación Animal , Animales , Enfermedad Hepática Inducida por Sustancias y Drogas/dietoterapia , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Modelos Animales de Enfermedad , Hígado/efectos de los fármacos , Hígado/metabolismo , Hígado/patología , Venenos/toxicidad , Enfermedades de las Aves de Corral/inducido químicamente , Enfermedades de las Aves de Corral/metabolismoRESUMEN
In the present study, the ability of aflatoxin B(1) (AFB(1) ) to induce endogenous oxidative damages and the possible ameliorating effects of dietary melatonin (MEL) were investigated. Newly hatched broiler chicks (n = 240) were fed aflatoxin-contaminated diets (0.5 or 1 mg kg(-1) diet) with or without MEL (40 mg kg(-1) bw) supplementation in diet for 40 days. AFB(1) resulted in a significant increase in lipid peroxidation (LPO) in the liver and erythrocytes accompanied with suppression of superoxide dismutase (SOD) and catalase (CAT) enzyme activities of erythrocytes. It also caused a significant reduction in levels of serum proteins and marked elevation in activities of serum transaminases. AFB(1) treatment also decreased both humoral and cell-mediated immune responses in growing chicks. Simultaneous administration of MEL with AFB(1) resulted in an obvious improvement in all the tested parameters. Long-term rather than short-term administration of MEL was more effective in rendering protection against AFB(1)-induced toxicity.
Asunto(s)
Aflatoxina B1/toxicidad , Antioxidantes/uso terapéutico , Sistema Inmunológico/efectos de los fármacos , Melatonina/uso terapéutico , Venenos/toxicidad , Animales , Animales Recién Nacidos , Antioxidantes/administración & dosificación , Pollos/metabolismo , Dieta , Relación Dosis-Respuesta a Droga , Peroxidación de Lípido/efectos de los fármacos , Masculino , Melatonina/administración & dosificación , Estrés Oxidativo/efectos de los fármacos , Intoxicación/tratamiento farmacológicoRESUMEN
The Yunnan region of China produces a distinctive variety of Pu'er tea, which is consequently labeled as a Chinese geographic indication product. In this study, the safety of Chinese Yunnan Pu'er tea with regard to heavy metal content was evaluated in 30 different brands of Pu'er tea, including 150 commercial samples. Metal levels in the Pu'er tea samples followed the order: copper (12-22 µg/g) > lead (0.26-3.2 µg/g) > arsenic (0.035-0.24 µg/g) > cadmium (0.0059-0.085 µg/g) > mercury (<0.010 µg/g). Mercury was not detected in 17 of the brands of Pu'er tea. Metal-to-metal correlation studies showed that there were no significant correlation between metal pairs. Based on current safety standards, the low levels of metals detected in these Pu'er tea samples mean they are safe for human consumption.