RESUMEN
BACKGROUND: New strategies are needed to combat multidrug-resistant bacteria. The restriction of iron uptake by bacteria is a promising way to inhibit their growth. We aimed to suppress the growth of Vibrio bacterial species by inhibiting their ferric ion-binding protein (FbpA) using food components. METHODS: Twenty spices were selected for the screening of FbpA inhibitors. The candidate was applied to antibacterial tests, and the mechanism was further studied. RESULTS: An active compound, rosmarinic acid (RA), was screened out. RA binds competitively and more tightly than Fe3+ to VmFbpA, the FbpA from V. metschnikovii, with apparent KD values of 8 µM vs. 17 µM. Moreover, RA can inhibit the growth of V. metschnikovii to one-third of the control at 1000 µM. Interestingly, sodium citrate (SC) enhances the growth inhibition effect of RA, although SC only does not inhibit the growth. The combination of RA/SC completely inhibits the growth of not only V. metschnikovii at 100/100 µM but also the vibriosis-causative pathogens V. vulnificus and V. parahaemolyticus, at 100/100 and 1000/100 µM, respectively. However, RA/SC does not affect the growth of Escherichia coli. CONCLUSIONS: RA/SC is a potential bacteriostatic agent against Vibrio species while causing little damage to indigenous gastrointestinal bacteria.
Asunto(s)
Cinamatos/farmacología , Depsidos/farmacología , Hierro/metabolismo , Citrato de Sodio/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Sitios de Unión , Cinamatos/química , Cinamatos/metabolismo , Depsidos/química , Depsidos/metabolismo , Sinergismo Farmacológico , Proteínas de Unión a Hierro/química , Proteínas de Unión a Hierro/metabolismo , Simulación del Acoplamiento Molecular , Extractos Vegetales/química , Unión Proteica , Vibrio parahaemolyticus/metabolismo , Ácido RosmarínicoRESUMEN
Isoquinoline alkaloids (IQs) from Macleaya cordata are promising natural products for enhancing the growth performance and overall health condition of farmed animals. The present study aimed to investigate the effects of two formulas of IQ extract, provided in either a powdered formula (IQ-E) or a water-soluble, granulated formula (IQ-WS) and containing the main active component sanguinarine at a concentration of 0.5% and 1%, respectively, on the growth, survival, immune response, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei). In Experiment 1, the postlarvae were divided into five groups (four replicates/group and 100 shrimp/tank) and fed four times/day for 30 days with a control feed, IQ-E at 200 or 300 mg/kg of feed, or IQ-WS at 100 or 150 mg/kg of feed. In Experiment 2, the surviving shrimp from Experiment 1 were redistributed into six groups (four treatment groups as in Experiment 1 plus the positive and negative controls with four replicates/group and 30 shrimp/tank) and challenged with V. parahaemolyticus by immersion at a concentration of 103 colony-forming units (CFU)/mL and were fed with the same diets for another 14 days. The results revealed that all IQ-fed shrimp in Experiment 1 had significantly enhanced survival rates and immune parameters (total hemocyte count and phagocytic, phenoloxidase, and superoxide dismutase activities) compared to the control group, even though the growth performances were similar across all groups. In Experiment 2, all IQ-fed groups showed better growth performance and survival rates compared to the positive control. Other than in the positive control group, no histopathological lesions in the hepatopancreas and the intestine were found. In summary, the current study demonstrated the benefits of using IQs from M. cordata as feed additives for improving the growth performance, survival rate, immune responses, and resistance to vibriosis of Pacific white shrimp.
Asunto(s)
Alcaloides/uso terapéutico , Antibacterianos/uso terapéutico , Medicamentos Herbarios Chinos/uso terapéutico , Isoquinolinas/uso terapéutico , Papaveraceae , Penaeidae/efectos de los fármacos , Vibriosis/prevención & control , Vibriosis/veterinaria , Vibrio parahaemolyticus/efectos de los fármacos , Animales , Papaveraceae/química , Penaeidae/crecimiento & desarrollo , Penaeidae/inmunología , Penaeidae/microbiologíaRESUMEN
Vibrio parahaemolyticus and Escherichia coli are two major foodborne pathogens. In this paper, the antibiofilm activities of the ethanol extract of cinnamon against these two bacteria were studied in detail. The antibacterial activity and the MIC of the extract were determined, and the inhibition and removing effects of the extract on the biofilms of V. parahaemolyticus and E. coli were investigated. The biofilms stained with fluorescein isothiocyanate (FITC) and concanavalin A (Con A) were also observed by confocal laser scanning microscope (CLSM). The results indicated that the extract exhibited high antibacterial activity, with the MIC against V. parahaemolyticus and E. coli was 6.25 mg/mL. The effects on V. parahaemolyticus biofilm were significant with the inhibition rate of 75.46% at MIC, and the eradication rate of 93.26% at 32MIC, respectively. As to E. coli, the inhibition rate was 48.18% at MIC, and the eradication rate was 46.16% at 8MIC. Meanwhile, the extract could notably reduce the metabolic activities and the secretion of EPS in biofilm, it inhibited 78.57% EPS formation in V. parahaemolyticus biofilm at MIC, and eliminated 61.28% EPS in mature biofilm at 4MIC. CLSM images showed that the EPS of the treated biofilm became thinner and biofilm structure was looser, when compared with the untreated control. This study elucidated that the cinnamon extract was effective to prevent biofilm formation and eradicate mature biofilms of V. parahaemolyticus and E. coli.
Asunto(s)
Biopelículas/efectos de los fármacos , Cinnamomum zeylanicum/química , Escherichia coli/efectos de los fármacos , Extractos Vegetales/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Antibacterianos/farmacología , Pruebas de Sensibilidad Microbiana , Microscopía ConfocalRESUMEN
When the aquaculture water environment deteriorates or the temperature rises, shrimp are susceptible to viral or bacterial infections, causing a large number of deaths. This study comprehensively evaluated the effects of the oral administration of a chitosan-gentamicin conjugate (CS-GT) after Litopenaeus vannamei were infected with Vibrio parahaemolyticus, through nonspecific immunity parameter detection, intestinal morphology observation, and the assessment of microbial flora diversification by 16S rRNA gene sequencing. The results showed that the oral administration of CS-GT significantly increased total hemocyte counts and reduced hemocyte apoptosis in shrimp (p < 0.05). The parameters (including superoxide dismutase, glutathione peroxidase, glutathione, lysozyme, acid phosphatase, alkaline phosphatase, and phenoloxidase) were significantly increased (p < 0.05). The integrity of the intestinal epithelial cells and basement membrane were enhanced, which correspondingly alleviated intestinal injury. In terms of the microbiome, the abundances of Vibrio (Gram-negative bacteria and food-borne pathogens) in the water and gut were significantly reduced. The canonical correspondence analysis (CCA) showed that the abundances of Vibrio both in the water and gut were negatively correlated with CS-GT dosage. In conclusion, the oral administration of CS-GT can improve the immunity of shrimp against pathogenic bacteria and significantly reduce the relative abundances of Vibrio in aquaculture water and the gut of Litopenaeus vannamei.
Asunto(s)
Antibacterianos/farmacología , Quitosano/farmacología , Suplementos Dietéticos , Microbioma Gastrointestinal/efectos de los fármacos , Gentamicinas/farmacología , Intestinos/efectos de los fármacos , Penaeidae/efectos de los fármacos , Alimentos Marinos , Vibrio parahaemolyticus/efectos de los fármacos , Alimentación Animal , Animales , Acuicultura , Intestinos/inmunología , Intestinos/microbiología , Penaeidae/crecimiento & desarrollo , Penaeidae/inmunología , Penaeidae/microbiología , Vibrio parahaemolyticus/inmunología , Vibrio parahaemolyticus/patogenicidad , Microbiología del AguaRESUMEN
Vibrio parahaemolyticus (VP) is a marine bacterium that opportunistically caused foodborne gastroenteritis in human and some diseases in marine animals. The isolated strain of V. parahaemolyticus WS001 from Samut Sakhon, Thailand has a presence of ldh (~ 450 bp) toxA (~ 333 bp) and toxB (~ 1269 bp) genes which showed pathogenicity in shrimp. This strain is suspected as low pathogenicity in human due to the lack of tdh and trh genes for encoding thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), respectively. The shrimp pathogenic strain was tested and revealed the multi-antibiotic resistances but was susceptible to norfloxacin (10 µg/ml). Citrus peel extracts were examined because they are rich in bioactive compounds such as saponins, tannins, flavonoids, steroids, and alkaloids that are effective in anti-VP activities. The ethanolic peel extracts of Citrus aurantifolia (Christm.) Swingle and Citrus hystrix DC. were found to be more anti-VP effect than other solvent extracts by Agar disc diffusion method at an optimum concentration of 50 mg/ml and Broth micro-dilution method (MICs of 50-100 mg/ml and MBCs of 100-200 mg/ml). Thus, C. aurantifolia (Christm.) Swingle peel extract was a distinctive candidate for the development of alternative natural agent to control the spreading of diseases in shrimp.
Asunto(s)
Citrus/química , Extractos Vegetales/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/genética , Factores de Virulencia/genética , Animales , Antibacterianos/farmacología , Farmacorresistencia Bacteriana Múltiple , Pruebas de Sensibilidad Microbiana , Penaeidae/microbiología , Tailandia , Vibrio parahaemolyticus/patogenicidadRESUMEN
In México, the infusion of Jatropha vernicosa stem bark has been used in folk medicine for many clinical situations, but no reports were available about this particular species of Jatropha in fish of mammals. In this first screening report, the phytochemical, antioxidant profile and antimicrobial properties of aqueous J. vernicosa stem bark extract were explored against Vibrio parahaemolyticus, an opportunist fish pathogen. To evaluate the cytotoxicity and immunological effect for the possible application of aqueous J. vernicosa stem bark in aquaculture, this study assessed it by using Longfin yellowtail Seriola rivoliana leukocytes. The results showed that phytochemical composition of the J. vernicosa extract was rich in phenol, flavonoid, saponin, and coumarin compounds. The antioxidant capacity of hydroxyl radical and superoxide anion scavenging activities, iron-chelation activity and ß-carotene bleaching coupled to linoleic acid showed that J. vernicosa extracts had a moderate antioxidant effect compared with synthetic antioxidants (BHT, BHA and EDTA). No adverse effects were observed on spleen leukocytes (viability > 98%). Interestingly, J. vernicosa stem bark extract has immunostimulant and antioxidant effects, increasing phagocytosis, respiratory burns activity, and nitric oxide production, as well as superoxide dismutase and catalase activities. Additionally, J. vernicosa extract increased pro-inflammatory cytokine IL-1ß and suppressed anti-inflammatory IL-10 gene expression upon stimuli and V. parahaemolyticus challenge. Finally, the data confirms that J. vernicosa stem bark extract is non-cytotoxic, rich in bioactive compounds with antioxidant effects, capable of enhancing the immune system in leukocytes and with great potential to fight against opportunistic diseases, such as vibriosis in fish.
Asunto(s)
Enfermedades de los Peces/inmunología , Peces , Factores Inmunológicos/farmacología , Jatropha/química , Sustancias Protectoras/farmacología , Vibriosis/veterinaria , Vibrio parahaemolyticus/efectos de los fármacos , Animales , Factores Inmunológicos/administración & dosificación , Corteza de la Planta/química , Sustancias Protectoras/administración & dosificación , Vibriosis/inmunologíaRESUMEN
The well-known red color change plays a significant role in consumer acceptability of crustacean species. In this study, we described the purification of the red color-related protein named MjRCP75 from the shell of Marsupenaeus japonicus. It was a homogeneous monomer with molecular mass of 75â¯kDa and rich in α-helix conformation. The α-helix content decreased within the increasing of heating temperature and was transformed dominantly to ß types. Identification and structural analysis revealed that MjRCP75 belonged to hemocyanin family. The released pigment from heated MjRCP75 showed a λmax at 483â¯nm in acetone. MjRCP75 showed clearly antibacterial activity against Escherichia coli, Staphylococcus aureus, and Vibrio parahaemolyticus. These findings identify MjRCP75 as the red color-related protein in M. japonicus shell and reveal its involvement in antibacterial activities.
Asunto(s)
Exoesqueleto/química , Antibacterianos/farmacología , Proteínas de Artrópodos/química , Proteínas de Artrópodos/farmacología , Penaeidae/química , Animales , Antibacterianos/química , Proteínas de Artrópodos/aislamiento & purificación , Evaluación Preclínica de Medicamentos , Escherichia coli/efectos de los fármacos , Hemocianinas/química , Pruebas de Sensibilidad Microbiana , Peso Molecular , Pigmentos Biológicos/química , Conformación Proteica , Staphylococcus aureus/efectos de los fármacos , Vibrio parahaemolyticus/efectos de los fármacosRESUMEN
Acute hepatopancreatic necrosis disease (AHPND) is a new emerging bacterial disease that has been recently reported to cause mass mortalities in Pacific whiteleg shrimp Penaeus vannamei. Antibiotics have been used to treat bacterial diseases in shrimp, but most of them have been ineffective and have resulted in drug residues in the harvested shrimp products. In this study, an alternative approach was tested for its efficacy in controlling AHPND. The extract of rose myrtle Rhodomyrtus tomentosa seed, a traditional Vietnamese medicine, was tested for antibacterial effect against three AHPND bacterial strains in vitro (Vibrio parahaemolyticus [VPAHPND ] KC12.020, VPAHPND KC13.14.2, and V. harveyi KC13.17.5) and was further evaluated for its potential efficacy in prevention of AHPND in shrimp in vivo. The in vitro studies showed that the antibacterial activity of the R. tomentosa extract was dose dependent, with the strongest bacterial susceptibility (≥18.0 mm) at a concentration of around 3,500 µg/disc. The in vivo studies showed that after challenge with VPAHPND KC12.020, the survival rates for shrimp in the groups that received feed pellets supplemented with extract at 3.5% or 7.0% (survival ~48.9% and 52.2%, respectively) were significantly higher than the zero survival rate in the positive control group, which received feed without the extract. These results indicate that the use of the R. tomentosa extract as an alternative therapy for control of AHPND in shrimp could help to minimize disease outbreaks. As a result, the extract is further expected to reduce drug/chemical residues in shrimp products.
Asunto(s)
Antibacterianos/farmacología , Myrtaceae/química , Penaeidae/microbiología , Extractos Vegetales/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Animales , Hepatopáncreas/patología , Necrosis/patología , Extractos Vegetales/química , Semillas/químicaRESUMEN
Acute hepatopancreatic necrosis disease (AHPND), caused by a toxin-producing Vibrio parahaemolyticus strain, has become a serious threat to shrimp aquaculture. The need to regulate antibiotic use prompted the development of alternative ways to treat infections in aquaculture including the use of chicken egg yolk immunoglobulin (IgY) for passive immunization. This study evaluated the protective effect of IgY against AHPND infection in Litopenaeus vannamei (Boone). IgY was isolated from eggs laid by hens immunized with recombinant PirA-like (rPirA) and PirB-like (rPirB) toxins. Whole-egg powders having IgY specific to rPirA (anti-PirA-IgY) and rPirB (anti-PirB-IgY) and IgY from non-immunized hen (control-IgY) were mixed with basal diets at 20% concentrations and used to prefeed shrimp 3 days before the bacterial challenge test. Survival rates of the challenged shrimp fed the anti-PirA-IgY, anti-PirB-IgY and control-IgY diets were 86%, 14% and 0%, respectively. Only the feed containing anti-PirA-IgY protected shrimp against AHPND. Increasing the concentration of rPirA antigen to immunize hens and lowering the amount of egg powder in feeds to 10% consistently showed higher survival rates in shrimp fed with anti-PirA-IgY (87%) compared with the control (12%). These results confirm that addition of anti-PirA-IgY in feeds could be an effective prophylactic method against AHPND infection in shrimp.
Asunto(s)
Inmunización Pasiva , Inmunoglobulinas/inmunología , Penaeidae/inmunología , Vibrio parahaemolyticus/efectos de los fármacos , Alimentación Animal/análisis , Animales , Toxinas Bacterianas/toxicidad , Pollos , Dieta , Suplementos Dietéticos/análisis , Yema de Huevo/química , Inmunoglobulinas/administración & dosificación , Penaeidae/efectos de los fármacos , Penaeidae/microbiología , Vacunación , Vibrio parahaemolyticus/fisiologíaRESUMEN
Bacteria respond to host immunity for their proliferation and survival by cell-cell communications such as biofilm formation, bioluminescence, and secreting virulence factors. In the biofilm form, bacteria are more resistant to various antimicrobial treatments and withstand the host's immune system. The approaches of deciphering biofilm formation for treating bacterial infections are therefore highly desirable. Recently, we have reported that the ethanolic extract of the red seaweed Gracilaria fisheri (G. fisheri) enhanced immune activities and inhibited growth of the luminescent bacteria Vibrio harveyi in shrimp. We undertook the present research study in order to evaluate and compare the effectiveness of the ethanolic extract from G. fisheri and furanone, a known biofilm inhibitor, in inhibiting the formation of clinically important Vibrio biofilms. The results showed that sub-lethal concentrations of both the ethanolic extracts (5, 10 and 100⯵gâ¯ml-1) and furanone (5⯵M) inhibited biofilm formation by V. harveyi and Vibrio parahaemolyticus and also light production (luminescence) in V. harveyi. It is known that V. harveyi mediated light production via autoinducer AI-2 pathway, we further determined whether the inhibitory effect of the extract was involved the AI-2 signaling. The bioluminescence assay was conducted in an AI-2 deletion mutant V. harveyi. Supplementation of the AI-2 containing media with the extract or furanone impaired the light production in the mutant V. harveyi suggesting that the extract interfered AI-2 mediated light production similar to furanone. In vivo challenge study showed that the low concentrations (Sub MICs) of the ethanolic extract and furanone decreased bacterial adhesion and colonization in the surfaces of stomach lumen, down-regulated expression of a virulence factor, and protected shrimp against mortality from V. harveyi and V. parahaemolyticus infection. In conclusion, the present results suggest a potential application of the low concentrations of the ethanolic extract of G. fisheri as an efficient approach for treating biofilm-associated Vibrio diseases in aquacultures.
Asunto(s)
Furanos/farmacología , Gracilaria/química , Penaeidae/microbiología , Vibrio/efectos de los fármacos , Animales , Acuicultura , Biopelículas/efectos de los fármacos , Luminiscencia , Extractos Vegetales/farmacología , Vibrio parahaemolyticus/efectos de los fármacosRESUMEN
The effects of oral administration of enrofloxacin (ENR) and San-Huang-San (SHS), singly or in combination, on the survival performance, disease resistance, and immunity of Litopenaeus vannamei were investigated. After challenge with an AHPND-causing strain of Vibrio parahaemolyticus (VPAHPND), shrimp were immediately fed a drug-free diet, diets containing only ENR (20â¯mg·kg-1) or SHS (500â¯mg·kg-1) or diets containing low-dose (10â¯mg·kg-1 ENR + 250 mg ·kg-1 SHS), medium-dose (20â¯mg·kg-1 ENR + 500 mg ·kg-1 SHS), and high-dose (40â¯mg·kg-1 ENR + 1000 mg ·kg-1 SHS) drug combinations for 5 days. The cumulative shrimp mortality over 5 days after injection of VPAHPND in the ENR + SHS combination groups was significantly lower than that in the ENR or SHS alone groups (pâ¯<â¯0.05). Immune parameters, including the vibrio density, total hemocyte counts (THCs), hemocyanin (HEM) concentration, antibacterial activity, activity levels of lysozyme (LZM), acid phosphatase (ACP), alkaline phosphatase (AKP), and phenoloxidase (PO) in cell-free hemolymph, and the expression levels of the immune-related genes anti-lipopolysaccharide factor (ALF), cathepsin B (catB), crustin, lectin (Lec), lysozyme (LZM), and Toll-like receptor (TLR) in hemocytes were determined in the shrimp. The results showed that the shrimp in drug combination groups cleared more VPAHPND than that in the ENR or SHS group in the same time. The values for other immune parameters in the drug combination groups were higher than those in the ENR or SHS group (pâ¯<â¯0.05). Finally, in the histological examinations, the histological structural alignment and integrity of the hepatopancreatic tubules in the drug combination groups were better than that in the ENR and SHS groups. Under the experimental conditions, compared with ENR or SHS used alone, the combination use of ENR and SHS could improve immunity and disease resistance in shrimp after VPAHPND infection, and could reduce the use of ENR when the better therapeutic effect was achieved.
Asunto(s)
Antibacterianos/uso terapéutico , Medicamentos Herbarios Chinos/uso terapéutico , Enrofloxacina/uso terapéutico , Inmunidad Innata , Penaeidae/inmunología , Vibrio parahaemolyticus/efectos de los fármacos , Animales , Antibacterianos/administración & dosificación , Medicamentos Herbarios Chinos/administración & dosificación , Enrofloxacina/administración & dosificación , Inmunidad Innata/efectos de los fármacos , Necrosis/prevención & control , Penaeidae/efectos de los fármacosRESUMEN
Seven compounds including four undescribed fusaric acid derivatives, namely fusaricates H-K, and two undescribed γ-pyrone derivatives, named fusolanones A-B, as well as a known compound fusaric acid, were isolated from a mangrove endophytic fungus Fusarium solani. Fusaricates H-K represent the first cases of fusaric acid butanediol esters and are diastereoisomers. Their structures including absolute configurations were elucidated based on NMR, MS, chemical synthesis, chiral HPLC analysis and ECD calculations. The antibacterial activity of all undescribed compounds were tested and fusolanone B showed the best activity with MIC value 6.25⯵g/mL on Vibrio parahaemolyticus.
Asunto(s)
Antibacterianos/química , Antibacterianos/farmacología , Ácido Fusárico/análogos & derivados , Ácido Fusárico/química , Fusarium/química , Pironas/química , China , Cromatografía Líquida de Alta Presión , Evaluación Preclínica de Medicamentos/métodos , Endófitos/química , Espectroscopía de Resonancia Magnética , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Estereoisomerismo , Vibrio parahaemolyticus/efectos de los fármacos , HumedalesRESUMEN
Peracetic acid (PAA) is a common oxidative sanitizer that is used in the food industry against various microorganisms. Limited information on the response of bacteria to this biocide is available. This study investigates the molecular response of the prevalent seafood-borne pathogenic Vibrio parahaemolyticus to PAA using mutants of peroxide scavenging genes. Among katE1, katE2, katG1, katG2, ahpC1 and ahpC2, and their regulator oxyR gene mutants, oxyR and katE mutants were highly susceptible to PAA. The growth and lethality of V. parahaemolyticus were harmed by 15â¯ppm of PAA in the â³katE1E2 double mutant, and were significantly ameliorated in the presence of the katE1 gene in the wild-type strain and the gene-complementary strains that were pre-adapted in 2â¯ppm of PAA or 100⯵M hydrogen peroxide. The application of PAA to these strains induced the accumulation of reactive oxygen species. The reduction of the level of hydrogen peroxide and gene expression during this treatment was influenced by the presence of katE genes. This investigation confirmed the major role of katE1 and a compensatory role of katE2 in the resistance of V. parahaemolyticus to PAA, and demonstrated some minor differences in the responses of this bacterium against PAA and hydrogen peroxide.
Asunto(s)
Desinfectantes/farmacología , Peróxido de Hidrógeno/farmacología , Ácido Peracético/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Farmacorresistencia Bacteriana Múltiple/genética , Microbiología de Alimentos , Pruebas de Sensibilidad Microbiana , Alimentos Marinos/microbiología , Vibrio parahaemolyticus/genéticaRESUMEN
The anti-Vibrio activity of essential oils (EOs) of nine medicinal plants was tested against 28 Vibrio spp. isolated from diseased Fenneropenaeus indicus. EO of Nigella sativa exhibited anti-Vibrio activity against all Vibrio spp. and greater inhibition was noted for the isolate V2 which was identified as Vibrio parahaemolyticus Dahv2. Further, EO of N. sativa effectively inhibited V. parahaemolyticus Dahv2 with an inhibition zone of 23.9mm at 101.2µgml(-1). Moreover, EO of N. sativa revealed anti-biofilm activity at 101.2µgml(-1) against V. parahaemolyticus Dahv2 and inhibited the growth of V. parahaemolyticus Dahv2 at 100µgml(-1).In vivo experimental infection studies showed that the survival of Artemia spp. infected with V. parahaemolyticus Dahv2 at 1×10(3)cfuml(-1) was only 40%. However, the survival of Artemia spp. was significantly increased after challenge with 100µgml(-1) of EO of N. sativa. EO of N. sativa showed higher anti-oxidant potential and total phenol content than other EOs tested. The anti-oxidant activity of EO of N. sativa was highly correlated to their total phenolic contents (r=0.836, P<0.05). This observation suggests that EO of N. sativa protected the Artemia spp. after experimental infection of V. parahaemolyticus Dahv2.
Asunto(s)
Artemia/microbiología , Biopelículas/efectos de los fármacos , Aceites Volátiles/farmacología , Vibriosis , Vibrio parahaemolyticus/efectos de los fármacos , Animales , Antioxidantes/farmacología , Pruebas de Sensibilidad Microbiana , Nigella sativa/químicaRESUMEN
The marine foodborne enteropathogen Vibrio parahaemolyticus has four putative catalase genes. The functions of two katE-homologous genes, katE1 (VPA1418) and katE2 (VPA0305), in the growth of this bacterium were examined using gene deletion mutants with or without complementary genes. The growth of the mutant strains in static or shaken cultures in a rich medium at 37°C or at low temperatures (12 and 4°C), with or without competition from Escherichia coli, did not differ from that of the parent strain. When 175 µM extrinsic H2O2 was added to the culture medium, bacterial growth of the ΔkatE1 strain was delayed and growth of the ΔkatE1 ΔkatE2 and ΔkatE1 ΔahpC1 double mutant strains was completely inhibited at 37°C for 8 h. The sensitivity of the ΔkatE1 strain to the inhibition of growth by H2O2 was higher at low incubation temperatures (12 and 22°C) than at 37°C. The determined gene expression of these catalase and ahpC genes revealed that katE1 was highly expressed in the wild-type strain at 22°C under H2O2 stress, while the katE2 and ahpC genes may play an alternate or compensatory role in the ΔkatE1 strain. This study demonstrated that katE1 encodes the chief functional catalase for detoxifying extrinsic H2O2 during logarithmic growth and that the function of these genes was influenced by incubation temperature.
Asunto(s)
Catalasa/metabolismo , Oxidantes/toxicidad , Estrés Oxidativo , Vibrio parahaemolyticus/enzimología , Vibrio parahaemolyticus/crecimiento & desarrollo , Catalasa/genética , Medios de Cultivo/química , Escherichia coli/crecimiento & desarrollo , Eliminación de Gen , Peróxido de Hidrógeno/toxicidad , Temperatura , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/genéticaRESUMEN
The aim of this study is to investigate the biocompatibility and anti-Vibrio efficacy of green synthesized silver nanoparticles (AgNPs) using an aqueous leaf extract of Adathoda vasica (A. vasica). The green synthesized silver nanoparticles were characterized by UV-vis, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). A. vasica AgNPs showed significant antibacterial activity against Vibrio parahaemolyticus in agar bioassay and well diffusion method. Further, nanoparticles interactions with bacteria and its antibacterial activity were confirmed by CLSM analysis. In vivo evaluation results confirmed that synthesized A. vasica AgNPs had good antibacterial efficacy and also nontoxic to the Artemia nauplii.
Asunto(s)
Antibacterianos/metabolismo , Antibacterianos/farmacología , Género Justicia/química , Nanopartículas/toxicidad , Extractos Vegetales/química , Plata/metabolismo , Plata/farmacología , Antibacterianos/química , Microscopía Electrónica de Transmisión , Nanopartículas/química , Hojas de la Planta/química , Plata/química , Espectroscopía Infrarroja por Transformada de Fourier , Vibrio parahaemolyticus/efectos de los fármacos , Difracción de Rayos XRESUMEN
Vibrio parahaemolyticus is a common marine food-borne enteropathogen. In this study, we examined the antioxidative activity, growth, biofilm formation, and cell mobility of an oxyR deletion mutant and its genetically complementary strain of V. parahaemolyticus. oxyR is the regulator of catalase and ahpC genes. Protection against extrinsic H2O2 and against the organic peroxides cumene hydroperoxide and tert-butyl hydroperoxide was weaker in the deletion mutant than in its parent strain. Expression of the major functional antioxidative genes, ahpC1 and VPA1418, was markedly decreased in the oxyR mutant. Growth of this mutant on agar medium was significantly inhibited by autoclaved 0.25% glucose and by 0.25% dipotassium hydrogen phosphate, 0.5% monosaccharides (glucose, galactose, xylose, and arabinose), or 114.8 mM phosphates. The inhibition of the growth of this oxyR mutant by extrinsic peroxides, autoclaved sugars, and phosphates was eliminated by the complementary oxyR gene or by the addition of catalase to the autoclaved medium, while no inhibition of growth was observed when filter-sterilized sugars were used. The formation of biofilm and swimming mobility were significantly inhibited in the oxyR mutant relative to that in the wild-type strain. This investigation demonstrates the antioxidative function of oxyR in V. parahaemolyticus and its possible roles in biofilm formation, cell mobility, and the protection of growth in heated rich medium.
Asunto(s)
Proteínas Bacterianas/metabolismo , Biopelículas/crecimiento & desarrollo , Factores de Transcripción/metabolismo , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/fisiología , Proteínas Bacterianas/genética , Derivados del Benceno/farmacología , Biopelículas/efectos de los fármacos , Catalasa/metabolismo , Medios de Cultivo/química , Regulación Bacteriana de la Expresión Génica , Prueba de Complementación Genética , Glucosa/farmacología , Peróxido de Hidrógeno , Monosacáridos/farmacología , Peróxidos/farmacología , Fosfatos/farmacología , Eliminación de Secuencia , Factores de Transcripción/genética , Vibriosis/microbiología , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/crecimiento & desarrollo , terc-Butilhidroperóxido/farmacologíaRESUMEN
BACKGROUND: Recently isolated Vibrio parahaemolyticus strains have displayed multiple antibiotic resistance. Alternatives to conventional antibiotics are needed, especially for the multiple-antibiotic-resistant V. parahaemolyticus pandemic strain. METHODS: A bacteriophage, designated pVp-1, showed effective infectivity for multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus, including V. parahaemolyticus pandemic strains. The therapeutic potential of the phage was studied in a mouse model of experimental infection using a multiple-antibiotic-resistant V. parahaemolyticus pandemic strain. We monitored the survivability and histopathological changes, quantified the bacterial and phage titers during phage therapy, and observed the immune response induced by phage induction. RESULTS: Phage-treated mice displayed protection from a V. parahaemolyticus infection and survived lethal oral and intraperitoneal bacterial challenges. CONCLUSIONS: To the best of our knowledge, this is the first report of phage therapy in a mouse model against a multiple-antibioticresistant V. parahaemolyticus pandemic strain infection.
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Bacteriófagos/crecimiento & desarrollo , Terapia Biológica/métodos , Farmacorresistencia Bacteriana Múltiple , Vibriosis/microbiología , Vibriosis/terapia , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/virología , Animales , Modelos Animales de Enfermedad , Femenino , Humanos , Ratones , Ratones Endogámicos BALB C , Análisis de Supervivencia , Resultado del TratamientoRESUMEN
Dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. In this context, the validations of the traditional claim that the leaf extract of D. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. The bacterial biofilms were quantified by crystal violet staining. Among the 17 bacterial pathogens screened, the methanolic fraction of the leaf extract clearly demonstrated antibiofilm activity for Proteus mirabilis, Vibrio vulnificus, Aeromonas hydrophila, Shigella sonnei, Chromobacterium violaceum ATCC 12472, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio cholerae, and Proteus vulgaris. At biofilm inhibitory concentrations, biofilm formation was reduced by up to 70-90â%. Furthermore, the potential quorum-sensing activity of the leaf extract was tested by agar well diffusion using Chromobacterium violaceum (ATCC 12472 & CV O26) reporter strains. The inhibition of violacein production may be due to direct or indirect interference on QS by active constituents or the interactive effect of different phytocompounds present in the extracts. This is the first report on antibiofilm and QS activity of D. falcata leaf extracts, signifying the scope for development of complementary medicine for biofilm-associated infections.
Asunto(s)
Antibacterianos/farmacología , Biopelículas/efectos de los fármacos , Loranthaceae/química , Extractos Vegetales/farmacología , Hojas de la Planta/química , Percepción de Quorum/efectos de los fármacos , Aeromonas hydrophila/efectos de los fármacos , Chromobacterium/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Humanos , Medicina Tradicional , Proteus mirabilis/efectos de los fármacos , Proteus vulgaris/efectos de los fármacos , Pseudomonas aeruginosa/efectos de los fármacos , Shigella sonnei/efectos de los fármacos , Vibrio alginolyticus/efectos de los fármacos , Vibrio cholerae/efectos de los fármacos , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio vulnificus/efectos de los fármacosRESUMEN
Four new polyketides (1-4) were isolated from the fungus Paecilomyces variotii, which was derived from the jellyfish Nemopilema nomurai. The planar structures and relative configurations of these polyketides were elucidated on the basis of spectroscopic analyses, including 2D NMR experiments. The compounds showed inhibitory activity against pathogenic bacteria including methicillin-resistant Staphylococcus aureus 3089 and multi-drug-resistant Vibrio parahemolyticus 7001 with MIC values in the range 5-40 µg/mL.