RESUMEN
In this study, two hydrophilic polymers hydroxypropyl methyl cellulose and beta-cyclodextrin (ß-CD) are used to synthesize highly responsive and spongy polymeric matrices. Porous and stimulus-responsive polymeric network was developed to improve the solubility of acyclovir (ACV) at significant level. Grafting was successfully carried out by free radical polymerization technique. Spongy matrices were characterized by percentage entrapment efficiency, drug loading, solubility studies, FTIR, powder X-ray diffraction, TGA, DSC, XRD, SEM, swelling studies, and in vitro studies. Acute oral toxicity studies were conducted to determine the safety of oral administration of prepared HPMC-ßCD-g-poly(AMPS) formulation. Porous and spongy structures were depicted in SEM images. Complex formation and thermal stability of constituents and drug (ACV) were analyzed by FTIR, TGA, and DSC spectra. XRD analysis revealed reduction in acyclovir crystallinity in spongy matrices. Particle size of optimized formulation was found in the range of 197 ± 2.55 nm. The momentous difference with reference product committed that drug solubility and release characteristics were markedly enhanced by the developed spongy matrices. Toxicity studies endorsed that developed spongy matrices were non-toxic and compatible to biological system. The efficient method of preparation, enhanced solubility, excellent physico-chemical characteristics, high dissolution, and non-toxic HPMC-ßCD-g-poly(AMPS) spongy matrices may be a promising approach for oral delivery of poorly soluble drugs.
Asunto(s)
Acrilamidas/síntesis química , Aciclovir/síntesis química , Alcanosulfonatos/síntesis química , Derivados de la Hipromelosa/síntesis química , Polimerizacion , beta-Ciclodextrinas/síntesis química , Acrilamidas/administración & dosificación , Aciclovir/administración & dosificación , Administración Oral , Alcanosulfonatos/administración & dosificación , Animales , Antivirales/administración & dosificación , Antivirales/síntesis química , Evaluación Preclínica de Medicamentos/métodos , Derivados de la Hipromelosa/administración & dosificación , Masculino , Tamaño de la Partícula , Polímeros/administración & dosificación , Polímeros/síntesis química , Conejos , Solubilidad , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Difracción de Rayos X/métodos , beta-Ciclodextrinas/administración & dosificaciónRESUMEN
We present the development of surfactant-free, silica-free and fully biobased oil-in-water antimicrobial Pickering emulsions, based on the self-assembly of ß-cyclodextrin and phytoantimicrobial oils (terpinen-4-ol or carvacrol). Undecylenic acid (UA), derived from castor oil, can be used as bio-based drug to treat fungal infection, but is less effective than petroleum-based drugs as azole derivatives. To maximize its antifungal potential, we have incorporated UA in fully biobased Pickering emulsions. These emulsions are effective against fungi, Gram-positive and Gram-negative bacteria. The carvacrol emulsion charged with UA is +390 % and +165 % more potent against methicillin-resistant S. aureus (MRSA), compared to UA and azole-based commercial formulations. Moreover, this emulsion is up to +480 % more efficient that UA ointment against C. albicans. Finally, remarkable eradication of E. coli and MRSA biofilms was obtained with this environmental-friendly emulsion.
Asunto(s)
Antibacterianos/farmacología , Antifúngicos/farmacología , Cimenos/farmacología , Ácidos Undecilénicos/farmacología , beta-Ciclodextrinas/farmacología , Antibacterianos/síntesis química , Antibacterianos/química , Antifúngicos/síntesis química , Antifúngicos/química , Candida albicans/efectos de los fármacos , Aceite de Ricino/química , Cimenos/síntesis química , Cimenos/química , Relación Dosis-Respuesta a Droga , Emulsiones/síntesis química , Emulsiones/química , Emulsiones/farmacología , Escherichia coli/efectos de los fármacos , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Relación Estructura-Actividad , Ácidos Undecilénicos/síntesis química , Ácidos Undecilénicos/química , beta-Ciclodextrinas/síntesis química , beta-Ciclodextrinas/químicaRESUMEN
Cyclodextrins (CDs), as pharmaceutical excipients with excellent biocompatibility, non-immunogenicity, and low toxicity in vivo, are widely used to carry drugs by forming inclusion complexes for improving the solubility and stability of drugs. However, the limited space of CDs' lipophilic central cavity affects the loading of many drugs, especially with larger molecules. In this study, ß-CDs were modified by acetonization to improve the affinity for the chemotherapy drug doxorubicin (DOX), and doxorubicin-adsorbing acetalated ß-CDs (Ac-CD:DOX) self-assembled to nanoparticles, followed by coating with the amphiphilic zinc phthalocyanine photosensitizer ZnPc-(PEG)5 for antitumor therapy. The final product ZnPc-(PEG)5:Ac-CD:DOX was demonstrated to have excellent stability and pH-sensitive drug release characteristics. The cell viability and apoptosis assay showed synergistic cytotoxic effects of chemotherapy and phototherapy. The mechanism of cytotoxicity was analyzed in terms of intracellular reactive oxygen species, mitochondrial membrane potential, and subcellular localization. More importantly, in vivo experiments indicated that ZnPc-(PEG)5:Ac-CD:DOX possessed significant tumor targeting, prominent antitumor activity, and less side effects. Our strategy expands the application of CDs as drug carriers and provides new insights into the development of CD chemistry.
Asunto(s)
Antineoplásicos/uso terapéutico , Doxorrubicina/uso terapéutico , Portadores de Fármacos/uso terapéutico , Nanopartículas/uso terapéutico , Neoplasias/tratamiento farmacológico , Animales , Antineoplásicos/química , Apoptosis/efectos de los fármacos , Doxorrubicina/química , Portadores de Fármacos/síntesis química , Portadores de Fármacos/efectos de la radiación , Liberación de Fármacos , Sinergismo Farmacológico , Células Hep G2 , Humanos , Concentración de Iones de Hidrógeno , Indoles/síntesis química , Indoles/efectos de la radiación , Indoles/uso terapéutico , Isoindoles , Luz , Masculino , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Ratones , Mitocondrias/efectos de los fármacos , Nanopartículas/química , Nanopartículas/efectos de la radiación , Compuestos Organometálicos/síntesis química , Compuestos Organometálicos/efectos de la radiación , Compuestos Organometálicos/uso terapéutico , Fármacos Fotosensibilizantes/química , Fármacos Fotosensibilizantes/efectos de la radiación , Fármacos Fotosensibilizantes/uso terapéutico , Especies Reactivas de Oxígeno/metabolismo , Compuestos de Zinc , beta-Ciclodextrinas/síntesis química , beta-Ciclodextrinas/efectos de la radiación , beta-Ciclodextrinas/uso terapéuticoRESUMEN
Multifunctional nanoconjugates possessing an assortment of key functionalities such as magnetism, florescence, cell-targeting, pH and thermo-responsive features were developed for dual drug delivery. The novelty lies in careful conjugation of each of the functionality with magnetic Fe3O4 nanoparticles by virtue of urethane linkages instead of silica in a simple one pot synthesis. Further ß-cyclodextrin (CD) was utilized to carry hydrophobic as well as hydrophilic drug. Superlative release of DOX could be obtained under acidic pH conditions and elevated temperature, which coincides with the tumor microenvironment. Mathematical modelling studies revealed that the drug release kinetics followed diffusion mechanism for both hydrophobic drug and hydrophilic drug. A number of fluorophores onto a single nanoparticle produced a strong fluorescence signal to optically track the nanoconjugates. Enhanced internalization due to folate specificity could be observed by fluorescence imaging. Further their accumulation driven by magnet near tumor site led to magnetic hyperthermia. in vitro studies confirmed the nontoxicity and hemocompatibility of the nanoconjugates. Remarkable cell death was observed with drug-loaded nanoconjugates at very low concentrations in cancer cells. The internalization and cellular uptake of poor bioavailable anticancer agent curcumin were found to be remarkably enhanced on dosing the drug loaded nanoconjugates as compared to free curcumin. Site specific drug delivery due to folate conjugation and subsequent significant suppression in tumor growth was demonstrated by in vivo studies.
Asunto(s)
Antineoplásicos/uso terapéutico , Portadores de Fármacos/química , Nanoconjugados/química , Nanomedicina Teranóstica/métodos , beta-Ciclodextrinas/química , Animales , Carcinoma Hepatocelular/tratamiento farmacológico , Curcumina/química , Curcumina/uso terapéutico , Doxorrubicina/química , Doxorrubicina/uso terapéutico , Portadores de Fármacos/síntesis química , Portadores de Fármacos/toxicidad , Liberación de Fármacos , Femenino , Fluoresceínas/síntesis química , Fluoresceínas/química , Fluoresceínas/toxicidad , Fluorescencia , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/química , Colorantes Fluorescentes/toxicidad , Células HeLa , Humanos , Nanopartículas de Magnetita/química , Nanopartículas de Magnetita/toxicidad , Masculino , Ratones Endogámicos BALB C , Nanoconjugados/toxicidad , beta-Ciclodextrinas/síntesis química , beta-Ciclodextrinas/toxicidadRESUMEN
Dantrolene sodium (Da) is an effective skeletal muscle relaxant. However, its pharmacological effects are severely limited owing to its poor solubility and low oral bioavailability. In order to solve these problems, an inclusion complex using hydroxypropyl-ß-cyclodextrin (HP-ß-CD) to improve the oral bioavailability of Da was prepared successfully by freeze-drying. The prepared complex was characterized by Powder X-ray diffractometry (PXRD), Fourier transform infrared spectroscopy (FTIR) and evaluated by a dissolution test and a pharmacokinetic study. The results of PXRD and FTIR proved the formation of a complex between Da and HP-ß-CD. The dissolution rate of Da was markedly improved from inclusion complex with more than 90% being released within 5min. The in vivo pharmacokinetics of Da and dantrolene sodium-hydroxypropyl-ß-cyclodextrin (Da-HP-ß-CD) inclusion complex were investigated in rats using a UPLC/MS/MS method. The Cmax and AUC0-t of the Da-HP-ß-CD inclusion complex were 5- and 3-fold higher than that of the Da. These results suggested that the Da-HP-ß-CD inclusion complex markedly improved the dissolution rate and bioavailability of Da.
Asunto(s)
Dantroleno/sangre , Dantroleno/síntesis química , Espectrometría de Masa por Ionización de Electrospray/métodos , beta-Ciclodextrinas/sangre , beta-Ciclodextrinas/síntesis química , 2-Hidroxipropil-beta-Ciclodextrina , Administración Oral , Animales , Dantroleno/administración & dosificación , Combinación de Medicamentos , Composición de Medicamentos , Evaluación Preclínica de Medicamentos/métodos , Femenino , Ratas , Ratas Sprague-Dawley , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Difracción de Rayos X/métodos , beta-Ciclodextrinas/administración & dosificaciónRESUMEN
In this study, double-emulsion capsules (DECs) capable of concealing drug-incorporated targeted-supermolecules are developed to achieve "on-demand" supermolecule release and enhanced sequential targeting for magneto-chemotherapy. These water-in-oil-in-water DECs less than 200 nm in diameter are synthesized using a single component of PVA (polyvinyl alcohol) polymer and the magnetic nanoparticles, which are capable of encapsulating large quantities of targeted supermolecules composed of palitaxel-incorporated beta-cyclodextrin decorated by hyaluronic acid (HA, a CD44-targeting ligand) in the watery core. The release profiles (slow, sustained and burst release) of the targeted supermolecules can be directly controlled by regulating the high-frequency magnetic field (HFMF) and polymer conformation without sacrificing the targeting ability. Through an intravenous injection, the positive targeting of the supermolecules exhibited a 20-fold increase in tumor accumulation via the passive targeting and delivery of DECs followed by positive targeting of the supermolecules. Moreover, this dual-targeting drug-incorporated supermolecular delivery vehicle at the tumor site combined with magneto-thermal therapy suppressed the cancer growth more efficiently than treatment with either drug or supermolecule alone.
Asunto(s)
Antineoplásicos/farmacología , Sistemas de Liberación de Medicamentos/métodos , Hipertermia Inducida/métodos , Magnetismo/métodos , Nanocápsulas/química , beta-Ciclodextrinas/química , Animales , Emulsiones/química , Endocitosis , Células HeLa , Humanos , Ácido Hialurónico/síntesis química , Ácido Hialurónico/química , Cinética , Células MCF-7 , Ratones Desnudos , Nanocápsulas/ultraestructura , Tamaño de la Partícula , Coloración y Etiquetado , Difracción de Rayos X , Ensayos Antitumor por Modelo de Xenoinjerto , beta-Ciclodextrinas/síntesis químicaRESUMEN
Baicalein is a type of flavonoid isolated from the roots of a medicinal plant, Scutellaria baicalensis. Although it has attracted considerable attention due to its antiviral, anti-tumor, and anti-inflammatory activities, its limited aqueous solubility inhibits the clinical application of this flavonoid. The present study aimed to prepare and characterize a host-guest complex in an effort to improve the solubility and antioxidant activity of baicalein. The host molecule is a macrocyclic ß-cyclodextrin (ß-CD) functionalized with cysteine for a synergetic effect. The structure of the synthesized cysteinyl ß-CD was analyzed using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The inclusion complex with baicalein was studied by UV-vis, NMR spectroscopy, scanning electron microscopy, and X-ray powder diffractometry. The formed cysteinyl ß-CD/baicalein inclusion complex efficiently improved the solubility and antioxidant ability of baicalein. Therefore, we suggest that the present cysteinyl ß-CD is a potential host molecule for inclusion complexation and for bioavailability augmentation.
Asunto(s)
Antioxidantes/química , Antioxidantes/farmacología , Cisteína/química , Flavanonas/química , Flavanonas/farmacología , beta-Ciclodextrinas/química , Disponibilidad Biológica , Espectroscopía de Resonancia Magnética , Estructura Molecular , Solubilidad , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Difracción de Rayos X , beta-Ciclodextrinas/síntesis químicaRESUMEN
A novel mono-modified ß-cyclodextrin (ß-CD) consisting of 4-carbamoylmethoxy-benzoyl unit at the primary side was synthesized and its self-assembly behavior was determined by X-ray crystallography and NMR spectroscopy. The crystal structure shows a 'Yin-Yang'-like packing mode, in which the modified ß-CD exhibits a channel superstructure formed by a tail-to-tail dimer as the repeating motif with the substituted group embedded within the hydrophobic cavity of the facing ß-CD. The geometry of the substituted group is determined by the inclusion of the cavity and is further stabilized by two intermolecular hydrogen bonds between the carbonyl O atom and phenyl group. Furthermore, NMR ROESY investigation indicates that the self-assembly behavior of the substituted group within the ß-CD cavity is retained in aqueous solution, and the effective binding constant Ka was calculated to be 1330 M(-1) by means of (1)H NMR titration according to iterative determination.
Asunto(s)
Acetamidas/síntesis química , beta-Ciclodextrinas/síntesis química , Acetamidas/química , Conformación de Carbohidratos , Secuencia de Carbohidratos , Cristalografía por Rayos X , Modelos Moleculares , Datos de Secuencia Molecular , Soluciones , beta-Ciclodextrinas/químicaRESUMEN
Flavor plays an important role and has been widely used in foods. Encapsulation can prevent the loss of volatile aromatic ingredients, provide protection and enhance the stability of the flavor. Kinetic and thermodynamic parameters are helpful in understanding the mechanism of molecular recognition between hosts and guests. This work focused on the study of production of a sweet orange flavor-ß-cyclodextrin (CD) inclusion complex, and investigated the combination of flavor and ß-CD by thermogravimetric analysis. Pyrolysis characteristics, kinetic and thermodynamic parameters of the flavor-ß-CD inclusion complex were determined. The results showed that the flavor-ß-CD inclusion complexes can form large aggregates in water. During thermal degradation of blank ß-CD and flavor-ß-CD inclusion complex, three main stages can be distinguished. The thermogravimetric (TG) curve of blank ß-CD shows a leveling-off from room temperature to 250°C, while the TG curve of flavor-ß-CD inclusion complex is downward sloping in this temperature range.
Asunto(s)
Citrus sinensis , Aromatizantes/síntesis química , Aceites de Plantas/síntesis química , beta-Ciclodextrinas/síntesis química , Termogravimetría/métodosRESUMEN
Chemical hosts bind their guests by the same physical mechanisms as biomolecules and often display similarly subtle structure activity relationships. The cyclodextrins have found increasing application as inert, nontoxic carriers of active compounds in drug formulations. The present study was conducted to prepare inclusion complexes of chlorhexidine:ß-cyclodextrin (Cx:ß-cd), and evaluate their interactions with bacterial membrane through: scanning electron microscopy (SEM) and transmission electron microscopy (TEM); and measuring morphology alterations, roughness values, and cell weights by atomic force microscopy (AFM). It was found that the antimicrobial activity was significantly enhanced by cyclodextrin encapsulation. SEM analysis images demonstrated recognizable cell membrane structural changes and ultrastructural membrane swelling. By TEM, cellular alterations such as vacuolization, cellular leakage, and membrane defects were observed; these effects were enhanced at 1:3 and 1:4 Cx:ß-cd. In addition, AFM analysis at these ratios showed substantially more membrane disruption and large aggregates mixing with microorganism remains. In conclusion, nanoaggregates formed by cyclodextrin inclusion compounds create cluster-like structures with the cell membrane, possibly due to a hydrogen rich bonding interaction system with increasing surface roughness and possibly increasing the electrostatic interaction between cationic chlorhexidine with the lipopolysaccharides of Gram negative bacteria.
Asunto(s)
Membrana Celular/ultraestructura , Bacterias Gramnegativas/ultraestructura , Microscopía de Fuerza Atómica/métodos , Microscopía Electrónica de Transmisión/métodos , Nanopartículas/química , beta-Ciclodextrinas/síntesis química , Aggregatibacter actinomycetemcomitans/química , Aggregatibacter actinomycetemcomitans/ultraestructura , Membrana Celular/efectos de los fármacos , Clorhexidina/administración & dosificación , Clorhexidina/síntesis química , Evaluación Preclínica de Medicamentos/métodos , Bacterias Gramnegativas/efectos de los fármacos , Pruebas de Sensibilidad Microbiana/métodos , Microscopía Electrónica de Rastreo/métodos , Nanopartículas/administración & dosificación , Tamaño de la Partícula , beta-Ciclodextrinas/administración & dosificaciónRESUMEN
Thalidomide is emerging as a therapeutic agent with renewed clinical importance, presenting anti-inflammatory, immunomodulatory, and antineoplasic properties. In this work, we studied the complexation of thalidomide with cyclodextrins as a strategy to circumvent the poor aqueous solubility of the drug. Thalidomide-hydroxypropyl-ß-cyclodextrin complexes were obtained by kneading method and were characterized by differential scanning calorimetry, powder X-ray diffractometry, and scanning electronic microscopy. The aqueous solubility and in vitro dissolution of thalidomide were significantly improved through the complexation. Physicochemical analysis of the complexes in solid state revealed a decreased crystallinity of the complexed drug in comparison with free thalidomide. Thalidomide was able to dissociate from the complexes and permeates across intestinal epithelial Caco-2 cells with a favorable high permeability profile equivalent to that of the free drug. In summary, the present results suggest that thalidomide-hydroxypropyl-ß-cyclodextrin complexes could be regarded as a promising strategy for improving the gastrointestinal absorption of thalidomide.
Asunto(s)
Absorción Intestinal/fisiología , Talidomida/síntesis química , Talidomida/metabolismo , beta-Ciclodextrinas/síntesis química , beta-Ciclodextrinas/metabolismo , 2-Hidroxipropil-beta-Ciclodextrina , Células CACO-2 , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Humanos , Absorción Intestinal/efectos de los fármacos , Permeabilidad/efectos de los fármacos , Solubilidad/efectos de los fármacos , Talidomida/farmacología , Difracción de Rayos X , beta-Ciclodextrinas/farmacologíaRESUMEN
Microencapsulation of ethanol extract of tea was performed in this study. In order to microencapsulate, ß-cyclodextrin was used as wall material. Ethanol extract of tea was used as the core material. Microcapsules in the solid form were obtained by drying the emulsions. RSM showed that optimal processing parameters were as followings: core material/wall material 1/4, ß-cyclodextrin content 16%, stirring time 30 min and stirring temperature 200°C. Pharmacological activities of ß-cyclodextrin encapsulation tea extract were determined. It was found that ß-cyclodextrin encapsulation tea extract could enhance BMD, BMC and bone Ca, Zn and Cu contents. In addition, ß-cyclodextrin encapsulation tea extract could still reduce blood Ca contents. These results indicated that ß-cyclodextrin encapsulation tea extract was useful for improving bone quality in aged animals.
Asunto(s)
Composición de Medicamentos/métodos , Extractos Vegetales/síntesis química , Té/química , beta-Ciclodextrinas/síntesis química , Animales , Densidad Ósea/efectos de los fármacos , Calcio/metabolismo , Cromatografía Líquida de Alta Presión , Cobre/metabolismo , Etanol , Análisis de los Mínimos Cuadrados , Ratones , Extractos Vegetales/farmacología , Espectrofotometría Ultravioleta , Zinc/metabolismo , beta-Ciclodextrinas/farmacologíaRESUMEN
A poly(amidoamine) (PAA) copolymer with beta-cyclodextrin was obtained by polyaddition reaction of 6-deoxy-6-amino-beta-cyclodextrin (beta-CD-NH(2)) and 2-methylpiperazine to 2,2-bis(acrylamido)acetic acid in aqueous medium. This beta-CD/PAA copolymer bears beta-CD units along the macromolecular chain, is water-soluble and non-cytotoxic. The complexing capacity of beta-CD/PAA was determined using an antiviral drug, Acyclovir, as a model of poorly water-soluble drug. Complex formation was confirmed by means of DSC and FTIR analyses. beta-CD/PAA can solubilize up to 11% w/w of Acyclovir notably increasing the aqueous solubility of the drug. The in vitro release studies showed the dependence of Acyclovir release rate on the solution pH. The antiviral activity of Acyclovir beta-CD/PAA complex was evaluated against herpes simplex virus type I in cell cultures. The Acyclovir beta-CD/PAA complex exhibited a higher antiviral activity than the free drug.
Asunto(s)
Aciclovir , Antivirales , Portadores de Fármacos/química , Poliaminas/química , beta-Ciclodextrinas/química , Aciclovir/administración & dosificación , Aciclovir/química , Aciclovir/farmacología , Animales , Antivirales/administración & dosificación , Antivirales/química , Antivirales/farmacología , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Portadores de Fármacos/síntesis química , Evaluación Preclínica de Medicamentos , Herpesvirus Humano 1/efectos de los fármacos , Concentración de Iones de Hidrógeno , Poliaminas/síntesis química , Solubilidad , Células Vero , beta-Ciclodextrinas/síntesis químicaRESUMEN
A 6A,6A'-dicyclohexylamine-6B,6B'-diselenide-bis-beta-cyclodextrin (6-CySeCD) was designed and synthesized to imitate the antioxidant enzyme glutathione peroxidase (GPX). In this novel GPX model, beta-cyclodextrin provided a hydrophobic environment for substrate binding within its cavity, and a cyclohexylamine group was incorporated into cyclodextrin in proximity to the catalytic selenium in order to increase the stability of the nucleophilic intermediate selenolate. 6-CySeCD exhibits better GPX activity than 6,6'-diselenide-bis-cyclodextrin (6-SeCD) and 2-phenyl-1,2-benzoisoselenazol-3(2H)-one (Ebselen) in the reduction of H(2)O(2), tert-butyl hydroperoxide and cumenyl hydroperoxide by glutathione, respectively. A ping-pong mechanism was observed in steady-state kinetic studies on 6-CySeCD-catalyzed reactions. The enzymatic properties showed that there are two major factors for improving the catalytic efficiency of GPX mimics. First, the substrate-binding site should match the size and shape of the substrate and second, incorporation of an imido-group increases the stability of selenolate in the catalytic cycle. More efficient antioxidant ability compared with 6-SeCD and Ebselen was also seen in the ferrous sulfate/ascorbate-induced mitochondria damage system, and this implies its prospective therapeutic application.