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2.
J Nurs Scholarsh ; 54(2): 226-233, 2022 03.
Artículo en Inglés | MEDLINE | ID: mdl-35129290

RESUMEN

PURPOSE: To determine the research output of Caribbean nurses and midwives. METHODS: We searched the Scopus database to identify publications by Caribbean nurses and midwives during the period 2000-2020. Publications were included in the analysis if they had at least one author who was either a nurse or midwife and affiliated with a Caribbean geographic location. All publication types and languages were included in the analysis. Organization analysis and collaboration networks were created using the VOS Viewer application. FINDINGS: The number of Scopus publications by Caribbean nurses and midwives progressively grew from 22 in 2000 to 584 in 2020. Cuba recorded the highest number of nursing research publications (319) followed by Jamaica (92), and Puerto Rico (59). Most publishing institutions were universities. The University of the West Indies (Jamaica) ranked highest with 15.2% of publications, followed by Universidad de Ciencias Médicas de La Habana with 14.4% of publications, Universidad de Puerto Rico ranked third with 9.8% of publications. The majority of publications (83.6%) were peer reviewed research articles, while review articles accounted for 9.8% of publications. Six out of the ten journals that published most research done by Caribbean nurse researchers were Cuban journals which published a total of n = 250 (75.8%) articles. All six journals had no impact factor and had low cite scores. CONCLUSIONS: Our analysis of bibliometric indicators suggest that recent and steady growth in nursing and midwifery research in the Caribbean has had low visibility. Equipping nurses and midwives with the necessary knowledge and skills to lead, teach, and conduct high quality research through doctorate level education is an imperative for increasing research productivity among Caribbean nurses and midwives. CLINICAL RELEVANCE: Nursing and midwifery research is critical for evidence-based nursing and midwifery practice. High quality and context specific research evidence will enable Caribbean nurses and midwives to provide quality and culturally sensitive nursing and midwifery care and contribute to evidence informed policy decisions.


Asunto(s)
Partería , Investigación en Enfermería , Bibliometría , Región del Caribe , Femenino , Humanos , Embarazo , Publicaciones , Indias Occidentales
3.
Artículo en Inglés | MEDLINE | ID: mdl-33557252

RESUMEN

CONTEXT: The Caribbean diaspora in the United States is a diverse community that is afflicted with high morbidity and mortality due to preventable chronic diseases. OBJECTIVE: Our goal is to determine which culturally sensitive health and nutrition educational modalities have the highest efficacy for improving general health in the Caribbean diaspora. METHODS: A scoping literature review was performed on the MEDLINE, CINAHL, and Web of Science databases using terms related to health and nutrition in the Caribbean population. Original, peer-reviewed research published from 2010 to 2020, which took place in the U.S. and Caribbean countries, were included in our review. RESULTS: We identified a total of nine articles that met our inclusion criteria. Rate differences for individual education program features were calculated to assess the likelihood of a positive impact on diet, physical activity, and diabetes. CONCLUSION: Our review helps to identify key educational modalities targeting diabetes, diet, and physical activity levels that can be used to meet the health and nutritional needs of the Caribbean diaspora population.


Asunto(s)
Migración Humana , Terapia Nutricional , Región del Caribe , Educación en Salud , Estados Unidos , Indias Occidentales
4.
Eur J Sport Sci ; 21(4): 590-603, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32460638

RESUMEN

This study examined the perceived talent development environment (TDE) of youth track and field athletes within the Caribbean region and explores the relationship with their subjective wellbeing and burnout levels. A sample of 400 Caribbean youth track and field athletes (male = 198, and female = 202; age 13-20 years) participated in this cross-sectional study. Athletes responded to a survey which included the Talent Development Environment Questionnaire (TDEQ-5), the World Health Organization Wellbeing Index (WHO-5) and the Athlete Burnout Questionnaire (ABQ). Structural equation modelling showed that a positive and supportive TDE was associated with better athlete wellbeing and lower athlete burnout. Moreover, gender and hours of training moderated relationships of athletes' perceptions of their development environment with wellbeing and burnout levels respectively. Furthermore, there was a positive association between the support network and athlete wellbeing, while holistic quality preparation was negatively associated with athlete burnout. Development environments perceived as supportive and geared towards a holistic athlete-centred approach were associated with better athlete wellbeing and lower burnout.


Asunto(s)
Aptitud , Atletas/psicología , Agotamiento Psicológico/psicología , Medio Social , Atletismo/psicología , Adolescente , Factores de Edad , Atletas/clasificación , Agotamiento Psicológico/epidemiología , Región del Caribe/epidemiología , Estudios Transversales , Femenino , Estado de Salud , Humanos , Masculino , Salud Mental , Acondicionamiento Físico Humano/psicología , Acondicionamiento Físico Humano/estadística & datos numéricos , Factores Sexuales , Encuestas y Cuestionarios , Factores de Tiempo , Atletismo/clasificación , Atletismo/fisiología , Indias Occidentales/epidemiología , Adulto Joven
6.
Viruses ; 12(10)2020 09 29.
Artículo en Inglés | MEDLINE | ID: mdl-33003342

RESUMEN

Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.


Asunto(s)
Dioscorea/virología , Patología Molecular/métodos , Enfermedades de las Plantas/virología , Virus de Plantas/aislamiento & purificación , Saneamiento/métodos , Badnavirus/genética , Badnavirus/aislamiento & purificación , Virus de Plantas/genética , Reacción en Cadena de la Polimerasa/métodos , Potexvirus/genética , Potexvirus/aislamiento & purificación , Reproducibilidad de los Resultados , Indias Occidentales
7.
J Holist Nurs ; 38(3): 263-277, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31619120

RESUMEN

Purpose: The purpose of the study is to understand the meaning of folklore as a health patterning modality and to uncover its unique characteristics in the life-health process. Design: Hermeneutic phenomenological. Method: Max van Manen's methodology was used for this study. The sample was selected from the Afro-Caribbean American community in the United States. The criterion for selection was that participants used folklore healing practices for health and well-being on an ongoing basis. In-depth interviews were done. Purposive sampling with networking was done based on whether the participants used folk healing on a regular basis. Themes of the meaning of folklore healing practices were identified from participants' verbatim data. Findings: The meaning of folklore healing practices was interpreted as phenomena with six interconnected essential themes. Additionally, Barrett's nursing theory of power as knowing participation in change was used to reflect and understand the findings from a nursing perspective. Conclusion: The research findings have implications for nursing science, and the knowledge gleaned from the study may be applied to nursing practice.


Asunto(s)
Folclore/psicología , Acontecimientos que Cambian la Vida , Medicina Tradicional/normas , Adulto , Anciano , Femenino , Hermenéutica , Humanos , Masculino , Medicina Tradicional/métodos , Medicina Tradicional/psicología , Persona de Mediana Edad , Investigación Cualitativa , Estados Unidos , Indias Occidentales/etnología
8.
Environ Res ; 181: 108908, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-31759648

RESUMEN

This study measured the concentration of total mercury (THg) and selenium (Se), and calculated the Se:Hg molar ratios in the muscle, blubber, liver, and kidney of small cetaceans (false killer whale, Pseudorca crassidens; killer whale, Orcinus orca; Risso's dolphin, Grampus griseus; short-finned pilot whale, Globicephala macrorhynchus; and dolphins of the genus Stenella) taken for human consumption off St. Vincent, West Indies. Overall, 122 samples were analyzed; mean THg concentrations (µg/g dry weight) were highest in the liver (730), followed by the kidney (274), muscle (76.4), and blubber (4.57). To explain variability in muscle THg concentrations, carbon (δ13C) and nitrogen (δ15N) stable isotope ratios were analyzed to explore differences in dietary carbon source and relative trophic position, respectively, among species. There was no relationship between δ15N and THg concentration, but there was a positive relationship between δ13C and THg concentration. On average for each species, the Se:Hg molar ratios were >1 in blubber and <1 in muscle. All liver samples and the majority of kidney, muscle, and blubber samples exceeded the FAO/WHO human consumption advisory level of 1 µg/g wet weight. Based on our estimations, consuming only 6.6 g of muscle a week would exceed the MeHg provisional tolerable weekly intake of 1.6 µg MeHg/kg body weight/week for a 60 kg person. Given the high THg concentration in these cetaceans and the frequency at which these tissues are consumed, this is a potential human health issue that warrants further investigation.


Asunto(s)
Delfines , Mercurio , Selenio , Contaminantes Químicos del Agua , Animales , Monitoreo del Ambiente , Humanos , Indias Occidentales
9.
Rev Neurol (Paris) ; 175(10): 641-643, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31519303

RESUMEN

In the 90's, clinico pathological studies have considerably improved the diagnosis of specific and rare neurodegenerative diseases. After a training in Parkinsons' disease in Paris, the author moved to French West Indies (Guadeloupe) and observed a high incidence of atypical parkinsonism with dementia, unresponsive to levodopa. Similar features were observed in Martinique. An environmental origin has been suspected with the exposure to toxins of annonaceae leaves and seeds. The candidate toxins are acetogenins acting as mitochondrial poison. This was demonstrated in neuronal cell cultures, and in animals. However, the agency for food security did not conclude that Annonaceae should not be used for herbal (medicinal) tea, even if the population is now aware about the possible risk of parkinsonism after exposure to annonaceae acetogenins.


Asunto(s)
Annonaceae/química , Demencia , Alimentos/toxicidad , Trastornos Parkinsonianos , Tés de Hierbas/toxicidad , Región del Caribe/epidemiología , Demencia/complicaciones , Demencia/epidemiología , Demencia/etiología , Resistencia a Medicamentos , Guadalupe/epidemiología , Humanos , Levodopa/uso terapéutico , Martinica/epidemiología , Enfermedad de Parkinson/clasificación , Enfermedad de Parkinson/epidemiología , Enfermedad de Parkinson/etiología , Trastornos Parkinsonianos/complicaciones , Trastornos Parkinsonianos/epidemiología , Trastornos Parkinsonianos/etiología , Indias Occidentales/epidemiología
11.
Chemosphere ; 199: 35-43, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29428514

RESUMEN

Toxicological screening of Swietenia mahagoni Jacq. (Meliaceae, West Indies Mahogany) against the lepidopteran pest Spodoptera litura was examined. Phytochemical screening through GC-MS analysis revealed nine peaks with prominent peak area % in Bis (2-ethylhexyl) phthalate (31.5%) was observed. The larvae exposed to discriminating dosage of 100 ppm deliver significant mortality rate compare to other treatment concentrations. The lethal concentrations (LC50 and LC90) was observed at the dosage of 31.04 and 86.82 ppm respectively. Sub-lethal concentrations (30 ppm) showed higher larval and pupal durations. However, pupal weight and mean fecundity rate reduced significantly. Similarly, the adult longevity reduced significantly in dose dependent manner. Midgut histology studies showed that the methanolic extracts significantly disturbs the gut epithelial layer, lumen and brush border membrane compare to the control. The soil assay on a non-target beneficial organism, the soil indicator earthworm Eudrilus eugeniae, with extracts from S. mahagoni (200 mg/kg) showed no toxicity compared to Monocrotophos at the dosage of 10 ppm/kg. Current results suggest that this bio-rational plant product from S. mahagoni displays a significant effect to reduce lepidopteran pests with low toxicity to other beneficial species.


Asunto(s)
Meliaceae/toxicidad , Oligoquetos/efectos de los fármacos , Spodoptera/efectos de los fármacos , Animales , Cromatografía de Gases y Espectrometría de Masas , Insecticidas/farmacología , Larva/efectos de los fármacos , Fitoquímicos/análisis , Extractos Vegetales/toxicidad , Pupa/efectos de los fármacos , Indias Occidentales
12.
Zoo Biol ; 34(1): 46-52, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25255994

RESUMEN

Conservation breeding programmes are a tool used to prevent amphibian extinctions. The husbandry requirements of amphibians are complex. Ongoing research is needed to ensure optimal management of those captive-bred animals destined, in particular, for reintroduction. The UV-B and vitamin D3 requirements of amphibians are largely unknown. Metabolic bone disease has been reported in a number of species. These include the Critically Endangered mountain chicken frog (Leptodactylus fallax) reared in captivity on diets supplemented with a high-calcium multivitamin and mineral supplement containing vitamin D3 but without UV-B provision. Captive-bred L. fallax being reared for reintroduction to Montserrat were provided with UV-B radiation from metamorphosis and were fed on insects supplemented with vitamins and minerals. Overlapping heat, light and UV-B gradients were provided, mimicking what we believe best represents the natural situation and thereby facilitated self-regulation of UV-B exposure. A subset of 10 frogs was periodically radiographed to assess skeletal health. Radiographic bone density and anatomical integrity appeared unremarkable when compared with a wild caught L. fallax. In addition to other routine health-screening, we recommend that radiography be performed to a structured schedule on a subset of all captive-bred and reared amphibians to assess skeletal health and to gauge the appropriateness of captive husbandry. We demonstrate here that, through the appropriate provision of a combination of both UV-B radiation and dietary supplementation, L. fallax can be bred and reared in captivity with healthy skeletal development.


Asunto(s)
Crianza de Animales Domésticos/métodos , Fenómenos Fisiológicos Nutricionales de los Animales , Animales de Zoológico , Anuros/fisiología , Enfermedades Óseas Metabólicas/veterinaria , Conservación de los Recursos Naturales/métodos , Terapia Ultravioleta/veterinaria , Animales , Enfermedades Óseas Metabólicas/prevención & control , Enfermedades Óseas Metabólicas/radioterapia , Suplementos Dietéticos , Terapia Ultravioleta/métodos , Indias Occidentales
16.
Animal ; 6(5): 763-77, 2012 May.
Artículo en Inglés | MEDLINE | ID: mdl-22558924

RESUMEN

Mixed farming systems (MFS) have demonstrated some success by focusing on the use of integrative and holistic mechanisms, and rationally building on and using the natural and local resource base without exhausting it, while enhancing biodiversity, optimizing complementarities between crops and animal systems and finally increasing opportunities in rural livelihoods. Focusing our analysis and discussion on field experiences and empirical knowledge in the Caribbean islands, this paper discusses the opportunities for a change needed in current MFS research-development philosophy. The importance of shifting from fragile/specialized production systems to MFS under current global conditions is argued with an emphasis on the case of Small Islands Developing States (SIDS) and the Caribbean. Particular vulnerable characteristics as well as the potential and constraints of SIDS and their agricultural sectors are described, while revealing the opportunities for the 'richness' of the natural and local resources to support authentic and less dependent production system strategies. Examples are provided of the use of natural grasses, legumes, crop residues and agro-industrial by-products. We analyse the requirement for a change in research strategies and initiatives through the development of a complex but necessary multi-/inter-/trans-disciplinary teamwork spirit. We stress as essential the collaboration and active participation of local and regional actors, stakeholders and end-users in the identification of research priorities, as well as the generation, exchange and dissemination of knowledge and technology innovations, while strengthening the leadership roles in the conduct of integrative and participative research and development projects.


Asunto(s)
Agricultura/métodos , Crianza de Animales Domésticos/métodos , Biodiversidad , Cruzamiento/métodos , Geografía , Investigación , Agricultura/economía , Alimentación Animal , Conducta Cooperativa , Factores Socioeconómicos , Clima Tropical , Indias Occidentales
17.
Am J Kidney Dis ; 58(4): 544-53, 2011 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-21803465

RESUMEN

BACKGROUND: Vitamin D (25 hydroxyvitamin D [25(OH)D]) deficiency is common in patients with chronic kidney disease (CKD). Neither the relation of this deficiency to the decrease in glomerular filtration rate (GFR) nor the effects on CKD mineral and bone disorders (MBD) are clearly established. STUDY DESIGN: Cross-sectional analysis of baseline data from a prospective cohort, the NephroTest Study. SETTING & PARTICIPANTS: 1,026 adult patients with all-stage CKD not on dialysis therapy or receiving vitamin D supplementation. PREDICTORS: For part 1, measured GFR (mGFR) using (51)Cr-EDTA renal clearance; for part 2, 25(OH)D deficiency at <15 ng/mL. OUTCOMES & MEASUREMENTS: For part 1, 25(OH)D deficiency and several circulating MBD markers; for part 2, circulating MBD markers. RESULTS: For part 1, the prevalence of 25(OH)D deficiency was associated inversely with mGFR, ranging from 28%-51% for mGFR ≥60-<15 mL/min/1.73 m(2). It was higher in patients of African origin; those with obesity, diabetes, hypertension, macroalbuminuria, and hypoalbuminemia; and during winter. After adjusting for these factors, ORs for 25(OH)D deficiency increased from 1.4 (95% CI, 0.9-2.3) to 1.4 (95% CI, 0.9-2.1), 1.7 (95% CI, 1.1-2.7), and 1.9 (95% CI, 1.1-3.6) as mGFR decreased from 45-59 to 30-44, 15-29, and <15 (reference, ≥60) mL/min/1.73 m(2) (P for trend = 0.02). For part 2, 25(OH)D deficiency was associated with higher age-, sex-, and mGFR-adjusted ORs of ionized calcium level <1.10 mmol/L (2.6; 95% CI, 1.2-5.9), 1,25 dihydroxyvitamin D concentration <16.7 pg/mL (1.8; 95% CI, 1.3-2.4), hyperparathyroidism (1.8; 95% CI, 1.3-2.4), and serum C-terminal cross-linked collagen type I telopeptides concentration >1,000 pg/mL (1.6; 95% CI, 1.0-2.6). It was not associated with hyperphosphatemia (phosphate >1.38 mmol/L). LIMITATIONS: Cross-sectional analysis of the data prevents causal inferences. CONCLUSIONS: 25(OH)D deficiency is related independently to impaired mGFR. Both mGFR decrease and 25(OH)D deficiency are associated with abnormal levels of circulating MBD biomarkers.


Asunto(s)
Enfermedades Óseas Metabólicas/epidemiología , Enfermedades Renales/epidemiología , Riñón/fisiopatología , Minerales/metabolismo , Deficiencia de Vitamina D/epidemiología , Adulto , África del Sur del Sahara/etnología , Anciano , Fosfatasa Alcalina/sangre , Biomarcadores , Enfermedades Óseas Metabólicas/sangre , Enfermedad Crónica , Estudios de Cohortes , Colágeno Tipo I/sangre , Comorbilidad , Estudios Transversales , Francia/epidemiología , Tasa de Filtración Glomerular , Humanos , Hiperparatiroidismo Secundario/sangre , Hiperparatiroidismo Secundario/epidemiología , Enfermedades Renales/sangre , Enfermedades Renales/fisiopatología , Persona de Mediana Edad , Orosomucoide/análisis , Péptidos/sangre , Prevalencia , Estudios Prospectivos , Vitamina D/análogos & derivados , Vitamina D/sangre , Deficiencia de Vitamina D/sangre , Indias Occidentales/etnología
18.
Midwifery ; 27(1): 36-41, 2011 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-19939527

RESUMEN

OBJECTIVE: to determine differences in antenatal care use between the native population and different ethnic minority groups in the Netherlands. DESIGN: the Generation R Study is a multi-ethnic population-based prospective cohort study. SETTING: seven midwife practices participating in the Generation R Study conducted in the city of Rotterdam. PARTICIPANTS: in total 2093 pregnant women with a Dutch, Moroccan, Turkish, Cape Verdean, Antillean, Surinamese-Creole and Surinamese-Hindustani background were included in this study. MEASUREMENTS: to assess adequate antenatal care use, we constructed an index, including two indicators; gestational age at first visit and total number of antenatal care visits. Logistic regression analysis was used to assess differences in adequate antenatal care use between different ethnic groups and a Dutch reference group, taking into account differences in maternal age, gravidity and parity. FINDINGS: overall, the percentages of women making adequate use are higher in nulliparae than in multiparae, except in Dutch women where no differences are present. Except for the Surinamese-Hindustani, all women from ethnic minority groups make less adequate use as compared to the native Dutch women, especially because of late entry in antenatal care. When taking into account potential explanatory factors such as maternal age, gravidity and parity, differences remain significant, except for Cape-Verdian women. Dutch-Antillean, Moroccan and Surinamese-Creole women exhibit most inadequate use of antenatal care. KEY CONCLUSIONS: this study shows that there are ethnic differences in the frequency of adequate use of antenatal care, which cannot be attributed to differences in maternal age, gravidity and parity. Future research is necessary to investigate whether these differences can be explained by socio-economic and cultural factors. IMPLICATIONS FOR PRACTISE: clinicians should inform primiparous women, and especially those from ethnic minority groups, on the importance of timely antenatal care entry.


Asunto(s)
Actitud Frente a la Salud/etnología , Diversidad Cultural , Etnicidad/estadística & datos numéricos , Grupos Minoritarios/estadística & datos numéricos , Aceptación de la Atención de Salud/etnología , Atención Prenatal/organización & administración , Adulto , Estudios de Cohortes , Femenino , Humanos , Partería/estadística & datos numéricos , Marruecos/etnología , Países Bajos , Embarazo , Estudios Prospectivos , Suriname/etnología , Encuestas y Cuestionarios , Turquía/etnología , Indias Occidentales/etnología , Salud de la Mujer/etnología , Adulto Joven
19.
West Indian med. j ; West Indian med. j;59(6): 597-601, Dec. 2010. graf, tab
Artículo en Inglés | LILACS | ID: lil-672687

RESUMEN

OBJECTIVE: To assess the cytotoxic activities of crude extracts and solvent fractions of Spermacoce verticillata, Ficus pumila and Flemingia strobilifera against a MT-4 human leukaemia cancer cell line. METHODS: Crude extracts of dried leaves of S verticillata, F pumila and F strobilifera were made by exhaustive methanol extraction, fractions were obtained from sequential extraction of the crude extract using solvents of increasing polarity. Dose responses corresponding to cell survival following 72-hour exposure to the extracts were determined using a leukaemia cancer cell line (MT-4). Cell viability was assessed using the MTT[3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay reading absorbances at 570 nm. Comparisons were made with controls and cell survival, in each sample well, was determined based on the ratio ofthe absorbance ofthe sample to the control. RESULTS: Crude extracts of S verticillata, F pumila and F strobilifera displayed cytotoxicity and the IC50 values were 89 µ/ml, 131 µ/ml and 81 µ/ml, respectively. The petroleum ether and chloroform fractions ofthe crude extracts of S verticillata and F strobilifera showed potent cytotoxic activity but the highest cytotoxic activity was found in the chloroform and butanol fractions of F pumila with IC50 values of 23 µg/ml and 26 µg/ml, respectively. CONCLUSION: The crude extracts of S verticillata, F pumila and F strobilifera were shown to be cytotoxic to the leukaemia cell line, MT-4 and IC50 values were determined. Fractionation of the crude extracts by solvent-solvent extraction enabled determination of the active fractions and their IC50 values. We propose that cytotoxic activity may be due to antioxidant compounds previously isolated from these plants.


OBJETIVO: Evaluar las actividades citotóxica de extractos crudos y las fracciones solventes de Spermacoce verticillata, Ficus pumila y Flemingia strobilifera contra una línea celular de la leucemia humana MT4. MÉTODOS: Se obtuvieron extractos crudos de hojas secas de S verticillata, F pumila y F strobilifera mediante extracción exhaustiva con etanol, y se obtuvieron fracciones a partir de la extracción secuencial del extracto crudo mediante solventes de polaridad creciente. Se determinaron las respuestas a las dosis correspondientes a la sobrevivencia de las células luego de 72 horas de exposición a los extractos, usando una línea celular de leucemia (MT-4). La viabilidad celular fue evaluada usando lecturas de absorbancia a partir del ensayo MTT [3-(4, 5-dimetiltiazol-2-il)-2, 5-difenil tetrazolio bromuro] a 570 nm. Se hicieron comparaciones con los controles. La sobrevivencia celular en cada pozo de muestreo, fue determinada a partir de la tasa de absorbancia de la muestra con respecto al control. RESULTADOS: Los extractos crudos de S verticillata, F pumila y F strobilifera mostraron citotoxicidad y los valores IC50 fueron 89 µ/ml, 131 µ/ml y 81 µ/ml, respectivamente. El éter de petróleo y las fracciones de cloroformo de los extractos crudos de S verticillata y F strobilifera mostraron una potente actividad citotóxica, pero la actividad citotóxica más alta fue hallada en las fracciones de cloroformo y butanol de F pumila con valores IC50 de 23 µ/ml y 26 µ/ml, respectivamente. CONCLUSIÓN: Los extractos de S verticillata, F pumila y F strobilifera demostraron ser citotóxicos a la línea celular MT4 y IC50 se determinaron los valores. El fraccionamiento de los extractos crudos extractos mediante extracción solventes-solventes hizo posible la determinación de las fracciones activas y sus valores IC50. Sugerimos que la actividad citotóxica puede deberse a compuestos antioxidantes previamente aislados a partir de estas plantas.


Asunto(s)
Humanos , Antineoplásicos Fitogénicos/farmacología , Leucemia/tratamiento farmacológico , Fitoterapia , Extractos Vegetales/farmacología , Plantas Medicinales , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Fabaceae/química , Ficus/química , Medicina Tradicional , Hojas de la Planta , Rubiaceae/química , Indias Occidentales
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