RESUMO
Objective:To optimize the water extraction process of traditional Chinese medicine ( TMC) Qubai granule. Methods:The orthogonal test was used to study four influencing factors including water amount, soaking time, extraction time and extraction times with dry extract yielding rate and the content of ferulic acid as the evaluation indices. Results:The optimum extraction process was as follows:A2 B1 C2 D2 , namely adding 10-fold amount of water, without soaking in advance, extracting twice with 2 h for each time. Con-clusion:The process is simple, stable and reproducible, which provides basis for the industrial production.
RESUMO
Baicalin has emerged as a promising agent for the therapy of infectious diseases due to the increasing number of pathogenic microbial strains resistant to several antibiotics. In this study, we investigated the inhibitory activity of baicalin on Chlamydia infection in vitro. We found that baicalin blocked the infection of HeLa cells in vitro when added to the infected cells. In order to shed light on the inhibitory effects of baicalin on the Chlamydia-infected cells, the expression of RFX5 and Chlamydia protease-like activity factor (CPAF) mRNAs and proteins in the Chlamydia-infected cells were examined using Western blot and real-time RT-PCR analysis. The results demonstrated that RFX5 and CPAF were upregulated and downregulated, respectively, by baicalin. Because CPAF is responsible for degrading RFX5, it is suggested that CPAF is a primary target of baicalin and plays an important role in downregulating RFX5. In conclusion, our findings demonstrated that baicalin can effectively inhibit Chlamydia Trachomatis in HeLa cells and therefore can be considered a potential agent for the therapy of infectious diseases caused by C. trachomatis.
Assuntos
Antibacterianos/uso terapêutico , Infecções por Chlamydia/prevenção & controle , Chlamydia trachomatis/efeitos dos fármacos , Medicamentos de Ervas Chinesas/uso terapêutico , Flavonoides/uso terapêutico , Fitoterapia , Scutellaria baicalensis/química , Antibacterianos/farmacologia , Proteínas de Bactérias/metabolismo , Western Blotting , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Regulação para Baixo , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Flavonoides/farmacologia , Células HeLa , Humanos , RNA Mensageiro/metabolismo , Fatores de Transcrição de Fator Regulador X , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
In this study, we tested the ability of Baicalin to block Chlamydia trachomatis infection and found that the Baicalin blocked infection of Hep-2 cells. Then, we looked into the expression of RFX5 and CPAF gene in Chlamydia-infected cells. We found that RFX5 and CPAF were up-regulated and down-regulated respectively by Baicalin. Since CPAF is responsible for degrading RFX5, we suggest that CPAF was a primary target of Baicalin and played an important role in regulating RFX5. Our findings demonstrate that Baicalin can inhibit C. trachomatis effectively and therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases.