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Fish Physiol Biochem ; 46(4): 1375-1385, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32232615

RESUMO

This study investigated the effect of Lactobacillus acidophilus-fortified diets on growth performance, antioxidant profiles, and immunity-related gene expressions of common carp, Cyprinus carpio juveniles. Four isonitrogenous diets containing 0, 102, 104, or 106 cfu/mL/kg L. acidophilus were fed to 240 Cyprinus carpio juveniles (mean = 21.34 ± 1.85 g), allotted to 12 rectangular tanks in a completely randomized designed at 3% body weight for 56 days. Growth performance and nutrient utilization were evaluated using standard procedures. Intestinal villi were measured, antioxidant profiles were evaluated from blood sera, and immunity-related gene expressions were evaluated. The results revealed that fish fed dietary 106 had significantly higher weight gain, SGR, feed intake, and lower FCR. Also, villi length, width, and areas of absorption were significantly improved in a dose-dependent manner. Furthermore, innate immune profiles, superoxide dismutase, catalase, respiratory bust activity as well as transforming growth factor beta (TGFß), interleukin 8 (IL-8), and tumor necrosis factor alpha (TNF-alpha) were significantly stimulated. This study evoked that Lactobacillus acidophilus supplementation enhanced better growth performance, improved antioxidant profiles, and modulated expression of immune-related genes of common carp, Cyprinus carpio, than the fish fed control diet. Results show that fish fed fortified diets had better growth performance, improved antioxidant profiles, and modulated expression of immune-related genes.


Assuntos
Carpas/fisiologia , Lactobacillus acidophilus/fisiologia , Análise de Variância , Animais , Antioxidantes/metabolismo , Carpas/crescimento & desenvolvimento , Carpas/imunologia , Carpas/metabolismo , Catalase/metabolismo , Suplementos Nutricionais , Ingestão de Alimentos , Alimentos Fortificados , Expressão Gênica/imunologia , Peróxido de Hidrogênio/análise , Concentração de Íons de Hidrogênio , Intestinos/anatomia & histologia , Microvilosidades/ultraestrutura , Oxigênio/análise , Distribuição Aleatória , Explosão Respiratória , Temperatura , Água/normas , Aumento de Peso
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