A new MAP-Rasagiline conjugate reduces α-synuclein inclusion formation in a cell model.

BACKGROUND: Parkinson's disease (PD) is the second most common neurodegenerative disease of the elderly. Current therapies are only symptomatic, and have no disease-modifying effect. Therefore, disease progresses continuously over time, presenting with both motor and non-motor features. The precise molecular basis for PD is still elusive, but the aggregation of the protein alpha-synuclein (α-syn) is a key pathological hallmark of the disease and is, therefore, a major focus of current research. Considering the intrinsic properties of cell-penetrating peptides (CPPs) for mediating drug delivery of neurotherapeutics across the blood brain barrier (BBB), these might open novel opportunities for the development of new solutions for the treatment of brain-related aspects of PD and other neurodegenerative disorders. METHODS: Here, we synthesized solid-phase CPPs using an amphipathic model peptide (MAP) conjugated with the drug Rasagiline (RAS), which we named RAS-MAP, and evaluated its effect on α-syn inclusion formation in a human cell-based model of synucleinopathy. RESULTS: We found that treatment with RAS-MAP at low concentrations (1-3 µM) reduced α-syn aggregation in cells. CONCLUSIONS: For the first time, we report that conjugation of a current drug used in the therapy of PD with CPP reduces α-syn aggregation, which might prove beneficial in PD and other synucleinopathies.

[Factors associated with the involuntary pilgrimage for childbirth care in São Luís (Maranhão State) and Ribeirão Preto (São Paulo State), Brazil: a contribution from the BRISA cohort]. / Fatores associados à peregrinação para o parto em São Luís (Maranhão) e Ribeirão Preto (São Paulo), Brasil: uma contribuição da coorte BRISA.

The objectives of this study were to estimate the involuntary pilgrimage by women in labor in search of childbirth care and to identify factors associated with this endeavor in two Brazilian cities. This was a cross-sectional study nested in the BRISA birth cohort, whose sample consisted of 10,475 women admitted to the selected maternity hospitals for delivery in São Luís (Maranhão State) and Ribeirão Preto (São Paulo State). Interviews were held with questionnaires that contained sociodemographic and obstetric variables. Hierarchical modeling was used, and relative risk was calculated with Poisson regression. Involuntary pilgrimage during labor was more frequent in São Luís (35.8%) than in Ribeirão Preto (5.8%). In São Luís, factors associated with pilgrimage were: first pregnancy (RR = 1.19; 95%CI: 1.08-1.31) and schooling less than 12 complete years. However, age 35 years or older (RR = 0.65; 95%CI: 0.54-0.84) was associated with less pilgrimage. In Ribeirão Preto, such trekking for obstetric care was more frequent in women with high-risk pregnancies (RR = 2.45; 95%CI: 1.81-3.32) and those with gestational age less than 37 weeks (RR = 1.93; 95%CI: 1.50-2.50). Meanwhile, delivery with gestational age equal to or greater than 42 weeks was associated with less pilgrimage (RR = 0.57; 95%CI: 0.33-0.98). In both cities, poor women had to trek more in search of childbirth care and had no guarantee of care, even for those who had received prenatal care. The study revealed the lack of guarantee of universal and equitable access and highlighted the unequal access to childbirth care between Brazil's major geographic regions.

O objetivo deste trabalho foi estimar a peregrinação de gestantes no momento do parto e identificar os fatores associados a essa peregrinação em duas cidades brasileiras. Estudo seccional, aninhado à coorte de nascimento BRISA, cuja amostra foi composta por 10.475 gestantes admitidas nas maternidades selecionadas por ocasião do parto em São Luís (Maranhão) e Ribeirão Preto (São Paulo). Entrevistas foram realizadas utilizando-se questionários que continham variáveis sociodemográficas e relacionadas ao parto. Utilizou-se modelagem hierarquizada, e calculou-se o risco relativo utilizando regressão de Poisson. A peregrinação foi mais frequente em São Luís (35,8%) que em Ribeirão Preto (5,8%). Em São Luís, foram fatores associados à maior peregrinação: ser primípara (RR = 1,19; IC95%: 1,08-1,31) e ter escolaridade menor que 12 ou mais anos de estudo. Entretanto, ter 35 anos ou mais (RR = 0,65; IC95%: 0,54-0,84) foi fator associado à menor peregrinação. Em Ribeirão Preto, peregrinaram com maior frequência as gestantes cujos partos foram de alto risco (RR = 2,45; IC95%: 1,81-3,32) e com idade gestacional inferior a 37 semanas (RR = 1,93; IC95%: 1,50-2,50). No entanto, partos com idade gestacional igual ou acima de 42 semanas foi um fator associado à menor peregrinação (RR = 0,57; IC95%: 0,33-0,98). Nas duas cidades, gestantes pobres peregrinaram com maior frequência, e sem garantia de que seriam atendidas, mesmo dentre as que realizaram o pré-natal. O estudo evidenciou ausência da garantia de acesso universal e equânime e reafirmou a desigualdade de acesso à assistência ao parto entre as regiões brasileiras.

El objetivo de este trabajo fue estimar los desplazamientos largos de gestantes en el momento del parto e identificar los factores asociados a estos desplazamientos en dos ciudades brasileñas. Se trata de una investigación seccional, encajada en la cohorte de nacimiento BRISA, cuya muestra estuvo compuesta por 10.475 gestantes, admitidas en las maternidades seleccionadas para dar a luz en São Luís (Maranhão) y Ribeirão Preto (São Paulo). Las entrevistas se realizaron utilizando cuestionarios que contenían variables sociodemográficas y relacionadas con el parto. Se utilizó un modelado jerarquizado, y se calculó el riesgo relativo utilizando la regresión de Poisson. Los desplazamientos fueron más frecuentes en São Luís (35,8%) que en Ribeirão Preto (5,8%). En São Luís, los factores asociados a mayores desplazamientos fueron: ser primípara (RR = 1,19; IC95%: 1,08-1,31) y contar con una escolaridad menor a 12 o más años de estudio. Sin embargo, tener 35 años o más (RR = 0,65; IC95%: 0,54-0,84) fue un factor asociado a menores desplazamientos. En Ribeirão Preto, se desplazaron con mayor frecuencia las gestantes cuyos partos fueron de alto riesgo (RR = 2,45; IC95%: 1,81-3,32) y con una edad gestacional inferior a 37 semanas (RR = 1,93; IC95%: 1,50-2,50). Sin embargo, los partos con una edad gestacional igual o por encima de las 42 semanas fue un factor asociado a un menor desplazamiento (RR = 0,57; IC95%: 0,33-0,98). En las dos ciudades, las gestantes pobres se desplazaron con mayor frecuencia, y sin garantía de que serían atendidas, incluso entre quienes realizaron seguimiento prenatal. El estudio evidenció la inexistencia de garantías de acceso universal y ecuánime a la salud y reafirmó la desigualdad de acceso en la asistencia al parto entre regiones brasileñas.

Fatores associados à peregrinação para o parto em São Luís (Maranhão) e Ribeirão Preto (São Paulo), Brasil: uma contribuição da coorte BRISA / Factors associated with the involuntary pilgrimage for childbirth care in São Luís (Maranhão State) and Ribeirão Preto (São Paulo State), Brazil: a contribution from the BRISA cohort / Factores asociados a largos desplazamientos para el parto en São Luís (Maranhão) y Ribeirão Preto (São Paulo), Brasil: la contribución de la cohorte BRISA

Resumo: O objetivo deste trabalho foi estimar a peregrinação de gestantes no momento do parto e identificar os fatores associados a essa peregrinação em duas cidades brasileiras. Estudo seccional, aninhado à coorte de nascimento BRISA, cuja amostra foi composta por 10.475 gestantes admitidas nas maternidades selecionadas por ocasião do parto em São Luís (Maranhão) e Ribeirão Preto (São Paulo). Entrevistas foram realizadas utilizando-se questionários que continham variáveis sociodemográficas e relacionadas ao parto. Utilizou-se modelagem hierarquizada, e calculou-se o risco relativo utilizando regressão de Poisson. A peregrinação foi mais frequente em São Luís (35,8%) que em Ribeirão Preto (5,8%). Em São Luís, foram fatores associados à maior peregrinação: ser primípara (RR = 1,19; IC95%: 1,08-1,31) e ter escolaridade menor que 12 ou mais anos de estudo. Entretanto, ter 35 anos ou mais (RR = 0,65; IC95%: 0,54-0,84) foi fator associado à menor peregrinação. Em Ribeirão Preto, peregrinaram com maior frequência as gestantes cujos partos foram de alto risco (RR = 2,45; IC95%: 1,81-3,32) e com idade gestacional inferior a 37 semanas (RR = 1,93; IC95%: 1,50-2,50). No entanto, partos com idade gestacional igual ou acima de 42 semanas foi um fator associado à menor peregrinação (RR = 0,57; IC95%: 0,33-0,98). Nas duas cidades, gestantes pobres peregrinaram com maior frequência, e sem garantia de que seriam atendidas, mesmo dentre as que realizaram o pré-natal. O estudo evidenciou ausência da garantia de acesso universal e equânime e reafirmou a desigualdade de acesso à assistência ao parto entre as regiões brasileiras.

Abstract: The objectives of this study were to estimate the involuntary pilgrimage by women in labor in search of childbirth care and to identify factors associated with this endeavor in two Brazilian cities. This was a cross-sectional study nested in the BRISA birth cohort, whose sample consisted of 10,475 women admitted to the selected maternity hospitals for delivery in São Luís (Maranhão State) and Ribeirão Preto (São Paulo State). Interviews were held with questionnaires that contained sociodemographic and obstetric variables. Hierarchical modeling was used, and relative risk was calculated with Poisson regression. Involuntary pilgrimage during labor was more frequent in São Luís (35.8%) than in Ribeirão Preto (5.8%). In São Luís, factors associated with pilgrimage were: first pregnancy (RR = 1.19; 95%CI: 1.08-1.31) and schooling less than 12 complete years. However, age 35 years or older (RR = 0.65; 95%CI: 0.54-0.84) was associated with less pilgrimage. In Ribeirão Preto, such trekking for obstetric care was more frequent in women with high-risk pregnancies (RR = 2.45; 95%CI: 1.81-3.32) and those with gestational age less than 37 weeks (RR = 1.93; 95%CI: 1.50-2.50). Meanwhile, delivery with gestational age equal to or greater than 42 weeks was associated with less pilgrimage (RR = 0.57; 95%CI: 0.33-0.98). In both cities, poor women had to trek more in search of childbirth care and had no guarantee of care, even for those who had received prenatal care. The study revealed the lack of guarantee of universal and equitable access and highlighted the unequal access to childbirth care between Brazil's major geographic regions.

Resumen: El objetivo de este trabajo fue estimar los desplazamientos largos de gestantes en el momento del parto e identificar los factores asociados a estos desplazamientos en dos ciudades brasileñas. Se trata de una investigación seccional, encajada en la cohorte de nacimiento BRISA, cuya muestra estuvo compuesta por 10.475 gestantes, admitidas en las maternidades seleccionadas para dar a luz en São Luís (Maranhão) y Ribeirão Preto (São Paulo). Las entrevistas se realizaron utilizando cuestionarios que contenían variables sociodemográficas y relacionadas con el parto. Se utilizó un modelado jerarquizado, y se calculó el riesgo relativo utilizando la regresión de Poisson. Los desplazamientos fueron más frecuentes en São Luís (35,8%) que en Ribeirão Preto (5,8%). En São Luís, los factores asociados a mayores desplazamientos fueron: ser primípara (RR = 1,19; IC95%: 1,08-1,31) y contar con una escolaridad menor a 12 o más años de estudio. Sin embargo, tener 35 años o más (RR = 0,65; IC95%: 0,54-0,84) fue un factor asociado a menores desplazamientos. En Ribeirão Preto, se desplazaron con mayor frecuencia las gestantes cuyos partos fueron de alto riesgo (RR = 2,45; IC95%: 1,81-3,32) y con una edad gestacional inferior a 37 semanas (RR = 1,93; IC95%: 1,50-2,50). Sin embargo, los partos con una edad gestacional igual o por encima de las 42 semanas fue un factor asociado a un menor desplazamiento (RR = 0,57; IC95%: 0,33-0,98). En las dos ciudades, las gestantes pobres se desplazaron con mayor frecuencia, y sin garantía de que serían atendidas, incluso entre quienes realizaron seguimiento prenatal. El estudio evidenció la inexistencia de garantías de acceso universal y ecuánime a la salud y reafirmó la desigualdad de acceso en la asistencia al parto entre regiones brasileñas.

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human's erythrocytes.

Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances - TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP, potentiate NPSH oxidation and hemolysis. Moreover, both extracts significantly prevents against t-BHP-induced TBARS production. A significant correlation among hemolysis and NPSH levels was found. Taking together, our data points that the association of M. pulegium extracts with t-BHP culminates in toxic effect to exposed erythrocytes, besides its protective effect against t-BHP-induced TBARS production. So, we infer that the use of this extract may exert negative effect during painful crisis in sickle cell anemia. However, more studies are still necessary to better investigate/understand the mechanism(s) involved in the toxic effect resultant from this association.

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human's erythrocytes

ABSTRACT Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances - TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP, potentiate NPSH oxidation and hemolysis. Moreover, both extracts significantly prevents against t-BHP-induced TBARS production. A significant correlation among hemolysis and NPSH levels was found. Taking together, our data points that the association of M. pulegium extracts with t-BHP culminates in toxic effect to exposed erythrocytes, besides its protective effect against t-BHP-induced TBARS production. So, we infer that the use of this extract may exert negative effect during painful crisis in sickle cell anemia. However, more studies are still necessary to better investigate/understand the mechanism(s) involved in the toxic effect resultant from this association.

Health Vulnerability Index and newborn hearing screening: urban inequality. / Índice de Vulnerabilidade à Saúde e triagem auditiva neonatal: diferenciais intraurbanos.

PURPOSE: To analyze the intra-urban differentials related to the outcome of the Newborn Hearing Screening (NHS) of children living in Belo Horizonte tested in a reference service using the Health Vulnerability Index (HVI). METHODS: cross-sectional study with children living in Belo Horizonte evaluated by a Newborn Hearing Screening Reference Service (NHSRS) between 2010 and 2011. The HVI of the census tract of each child was obtained by the georeferencing of their respective addresses. Multivariate analysis was conducted using the decision tree technique, considering a statistical model for each response. A thematic map of points representing the geographic distribution of the children evaluated by the NHS program was also developed. RESULTS: The NHS failure rate for children living in areas with very high HVI, or without HVI data, was 1.5 times higher than that for children living in other census tracts. For children living in areas of low, medium, and high HVI, who underwent NHS after 30 days of life, the NHS failure rate was 2.1 times higher in children that presented Risk Indicator for Hearing Loss (RIHL) (17.2%) than in those who did not (8.1%). Uneven distribution was observed between areas for children that underwent the NHS and those who failed it. CONCLUSION: Significant intra-urban differentials were found in Belo Horizonte, indicating correlation between health vulnerability and NHS outcomes.

Índice de Vulnerabilidade à Saúde e triagem auditiva neonatal: diferenciais intraurbanos / Health Vulnerability Index and newborn hearing screening: urban inequality

RESUMO Objetivo Analisar os diferenciais intraurbanos associados ao resultado da triagem auditiva neonatal (TAN) de crianças residentes em Belo Horizonte e avaliadas em um Serviço de Referência de TAN, tendo como referência o Índice de Vulnerabilidade à Saúde (IVS). Método Estudo observacional com amostra de crianças residentes em Belo Horizonte e avaliadas por um Serviço de Referência de TAN, entre 2010 e 2011. O IVS do setor censitário de cada criança foi obtido por georreferenciamento de seu respectivo endereço. Foi elaborado modelo estatístico para as variáveis respostas: “resultado da TAN”, “resultado no reteste”, “absenteísmo no reteste” e realizada análise multivariada, utilizando-se a técnica de árvore de decisão. Foi elaborado mapa temático de pontos para representar a distribuição espacial das crianças avaliadas pelo Programa, segundo seu resultado na TAN. Resultados A probabilidade de falhar na TAN para as crianças residentes em áreas de IVS muito elevado é 1,5 vez maior do que para as crianças residentes nas demais áreas. Para as crianças que residem em áreas de IVS baixo, médio e elevado e que fizeram a TAN após os 30 dias de vida, a probabilidade de falhar é 2,1 vezes maior nas crianças que apresentam indicador de risco para deficiência auditiva (17,2%), em relação às sem indicador de risco (8,1%). Observou-se também distribuição heterogênea de realização da TAN e de resultado da avaliação entre as regiões do município. Conclusão Foram evidenciados importantes diferenciais intraurbanos no Município de Belo Horizonte, indicando associação entre a vulnerabilidade à saúde e o resultado da TAN.

ABSTRACT Purpose To analyze the intra-urban differentials related to the outcome of the Newborn Hearing Screening (NHS) of children living in Belo Horizonte tested in a reference service using the Health Vulnerability Index (HVI). Methods cross-sectional study with children living in Belo Horizonte evaluated by a Newborn Hearing Screening Reference Service (NHSRS) between 2010 and 2011. The HVI of the census tract of each child was obtained by the georeferencing of their respective addresses. Multivariate analysis was conducted using the decision tree technique, considering a statistical model for each response. A thematic map of points representing the geographic distribution of the children evaluated by the NHS program was also developed. Results The NHS failure rate for children living in areas with very high HVI, or without HVI data, was 1.5 times higher than that for children living in other census tracts. For children living in areas of low, medium, and high HVI, who underwent NHS after 30 days of life, the NHS failure rate was 2.1 times higher in children that presented Risk Indicator for Hearing Loss (RIHL) (17.2%) than in those who did not (8.1%). Uneven distribution was observed between areas for children that underwent the NHS and those who failed it. Conclusion Significant intra-urban differentials were found in Belo Horizonte, indicating correlation between health vulnerability and NHS outcomes.

Quality indicators in a newborn hearing screening service / Indicadores de qualidade em um serviço de triagem auditiva neonatal

INTRODUCTION: Newborn hearing screening (NHS) programs are implemented across the globe to detect early hearing impairment. In order to meet this objective, the quality of these programs should be monitored using internationally recognized indicators. OBJECTIVE: To evaluate a newborn hearing screening service (NHSS) using international quality indicators. METHODS: A retrospective cohort study on the NHSS of Minas Gerais was conducted, analyzing the services performed between 2010 and 2011. Results were analyzed according to criteria from the American Academy of Pediatrics and the Joint Committee on Infant Hearing. RESULTS: This study assessed 6987 children. The proportions of cases that were referred for a retest, that followed through with retest, and that were referred for diagnosis were 8.0%, 71.9%, and 2.1%, respectively. The proportion of assessed newborn children in the first 30 days of life in this study was 65%. The median age of those children who failed both the NHS and the retest was significantly higher than the other children. The chance of a child with a hearing impairment risk indicator to fail the NHS was 2.4 times higher than of those without a risk indicator. CONCLUSION: NHSS achieved three of four evaluated indicators. Despite this, it is still necessary to perform NHS earlier and to ensure that the subsequent steps are followed. .

INTRODUÇÃO: Programas de Triagem Auditiva Neonatal (TAN) são implantados em todo mundo objetivando a detecção precoce da deficiência auditiva. A qualidade destes programas deve ser monitorada utilizando indicadores reconhecidos internacionalmente, para que este objetivo seja alcançado. OBJETIVO: Avaliar um Serviço de Triagem Auditiva Neonatal (STAN) com base nos indicadores internacionais de qualidade. MÉTODO: Coorte retrospectiva com análise dos atendimentos realizados por um STAN de Minas Gerais entre 2010 a 2011. Os resultados foram analisados segundo critérios da American Academy of Pediatrics e do Joint Committee on Infant Hearing. RESULTADOS: Foram avaliadas 6.987 crianças. As proporções de encaminhamento para reteste, adesão ao reteste e encaminhamento para diagnóstico foram 8,0%, 71,9% e 2,1%, respectivamente. A proporção de crianças avaliadas nos primeiros 30 dias de vida foi 65,0%. A mediana de idade das crianças que falharam na TAN e no reteste foi significativamente maior do que para as demais. O risco de uma criança com indicador de risco para deficiência auditiva (IRDA) falhar na TAN foi 2,4 vezes maior do que para as demais. CONCLUSÃO: O STAN alcançou 3 dos 4 indicadores avaliados. No entanto, ainda são necessários esforços para captação precoce de neonatos para a triagem auditiva e adesão às etapas subsequentes. .

Quality indicators in a newborn hearing screening service.

INTRODUCTION: Newborn hearing screening (NHS) programs are implemented across the globe to detect early hearing impairment. In order to meet this objective, the quality of these programs should be monitored using internationally recognized indicators. OBJECTIVE: To evaluate a newborn hearing screening service (NHSS) using international quality indicators. METHODS: A retrospective cohort study on the NHSS of Minas Gerais was conducted, analyzing the services performed between 2010 and 2011. Results were analyzed according to criteria from the American Academy of Pediatrics and the Joint Committee on Infant Hearing. RESULTS: This study assessed 6987 children. The proportions of cases that were referred for a retest, that followed through with retest, and that were referred for diagnosis were 8.0%, 71.9%, and 2.1%, respectively. The proportion of assessed newborn children in the first 30 days of life in this study was 65%. The median age of those children who failed both the NHS and the retest was significantly higher than the other children. The chance of a child with a hearing impairment risk indicator to fail the NHS was 2.4 times higher than of those without a risk indicator. CONCLUSION: NHSS achieved three of four evaluated indicators. Despite this, it is still necessary to perform NHS earlier and to ensure that the subsequent steps are followed.

TGF-ß1 expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding.

Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor ß(TGF-ß1 ) mRNA levels than E(C) rats (2·58 ± 0·83 versus 3·53 ± 0·57, P < 0·01) and in comparison with M(S) and M(A) rats (4·66 ± 2·49 and 4·61 ± 2·11, respectively; P < 0·05). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (0·74 ± 0·16 versus 1·25 ± 0·66; and 1·07 ± 0·45 versus 1·79 ± 0·89, respectively; P≤ 0·04), but did not differ from levels in E(C) and M(C) animals. In experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-ß1 genes, which was minimised by early enteral feeding with standard or supplemented diets.

[Quality of data on the Child Health Record and related factors]. / Qualidade do preenchimento da Caderneta de Saúde da Criança e fatores associados.

The Child Health Record (CHR) is an essential document for children's health care activities. A cross-sectional study was performed with simple random sampling, aimed at analyzing factors related to quality of data completion on the CHR. Interviews and direct verification of 365 CHRs from 9 health districts in Belo Horizonte, Minas Gerais State, Brazil, were performed. A scoring system was created to classify the CHRs in terms of quality of data completion and to relate them to explanatory variables. Odds ratios were calculated by logistic regression. Completion of the 20 scoring items varied from 3.1% (use of iron supplements) to 99.7% (date of birth). Factors associated with worse scores were: children > 12 months old (OR = 1.77), mothers with < or = 6 years of schooling (OR = 1.97), children not treated by general practitioners (OR = 3.18), and mothers who had not received explanations on the CHR while in the maternity ward (OR = 1.77). The results show poor use of the CHR and emphasize the need for on-going efforts to train health professionals and prepare health services for their important role in the promotion of children's health.

Qualidade do preenchimento da Caderneta de Saúde da Criança e fatores associados / Quality of data on the Child Health Record and related factors

A Caderneta de Saúde da Criança (CSC) é um documento imprescindível para a promoção da saúde infantil. Foi realizado um estudo transversal com amostra aleatória simples de crianças acompanhadas no Sistema Único de Saúde (SUS), visando analisar os fatores associados à qualidade do preenchimento da CSC. Foram realizadas entrevistas e verificação direta das cadernetas de 365 crianças das nove regionais de saúde de Belo Horizonte, Minas Gerais, Brasil. Foi criado um sistema de escore para classificar as CSC quanto à qualidade do seu preenchimento e relacioná-la às variáveis explicativas. O odds ratio foi calculado por regressão logística. O preenchimento dos vinte itens do escore variou de 3,1 por cento (uso de ferruginoso) a 99,7 por cento (data de nascimento). Os fatores associados aos piores escores foram: crianças > 12 meses de idade (OR = 1,77), mães < 6 anos de estudo (OR = 1,97), crianças não acompanhadas por médicos generalistas (OR = 3,18) e mães que não receberam explicações sobre a CSC na maternidade (OR = 1,77). Os resultados apontam a precária utilização da CSC, reforçando a necessidade de investimentos em capacitação dos profissionais e organização dos serviços para que ela cumpra seu papel na promoção da saúde infantil.

The Child Health Record (CHR) is an essential document for children's health care activities. A cross-sectional study was performed with simple random sampling, aimed at analyzing factors related to quality of data completion on the CHR. Interviews and direct verification of 365 CHRs from 9 health districts in Belo Horizonte, Minas Gerais State, Brazil, were performed. A scoring system was created to classify the CHRs in terms of quality of data completion and to relate them to explanatory variables. Odds ratios were calculated by logistic regression. Completion of the 20 scoring items varied from 3.1 percent (use of iron supplements) to 99.7 percent (date of birth). Factors associated with worse scores were: children > 12 months old (OR = 1.77), mothers with < 6 years of schooling (OR = 1.97), children not treated by general practitioners (OR = 3.18), and mothers who had not received explanations on the CHR while in the maternity ward (OR = 1.77). The results show poor use of the CHR and emphasize the need for on-going efforts to train health professionals and prepare health services for their important role in the promotion of children's health.

Effect of different polishing systems on the surface roughness of microhybrid composites

The use of composite resins in dentistry is well accepted for restoring anterior and posterior teeth. Many polishing protocols have been evaluated for their effect on the surface roughness of restorative materials. This study compared the effect of different polishing systems on the surface roughness of microhybrid composites. Thirty-six specimens were prepared for each composite $#91;Charisma® (Heraeus Kulzer), Fill Magic® (Vigodent), TPH Spectrum® (Dentsply), Z100® (3M/ESPE) and Z250® (3M/ESPE)] and submitted to surface treatment with Enhance® and PoGo® (Dentsply) points, sequential Sof-Lex XT® aluminum oxide disks (3M/ESPE), and felt disks (TDV) combined with Excel® diamond polishing paste (TDV). Average surface roughness (Ra) was measured with a mechanical roughness tester. The data were analyzed by two-way ANOVA with repetition of the factorial design and the Tukey-Kramer test (p<0.01). The F-test result for treatments and resins was high (p<0.0001 for both), indicating that the effect of the treatment applied to the specimen surface and the effect of the type of resin on surface roughness was highly significant. Regarding the interaction between polishing system and type of resin used, a p value of 0.0002 was obtained, indicating a statistically significant difference. A Ra of 1.3663 was obtained for the Sof-Lex/TPH Spectrum interaction. In contrast, the Ra for the felt disk+paste/Z250 interactions was 0.1846. In conclusion, Sof-Lex polishing system produced a higher surface roughness on TPH Spectrum resin when compared to the other interactions.

Effect of different polishing systems on the surface roughness of microhybrid composites.

The use of composite resins in dentistry is well accepted for restoring anterior and posterior teeth. Many polishing protocols have been evaluated for their effect on the surface roughness of restorative materials. This study compared the effect of different polishing systems on the surface roughness of microhybrid composites. Thirty-six specimens were prepared for each composite $#91;Charisma (Heraeus Kulzer), Fill Magic (Vigodent), TPH Spectrum (Dentsply), Z100 (3M/ESPE) and Z250 (3M/ESPE)] and submitted to surface treatment with Enhance and PoGo (Dentsply) points, sequential Sof-Lex XT aluminum oxide disks (3M/ESPE), and felt disks (TDV) combined with Excel diamond polishing paste (TDV). Average surface roughness (Ra) was measured with a mechanical roughness tester. The data were analyzed by two-way ANOVA with repetition of the factorial design and the Tukey-Kramer test (p<0.01). The F-test result for treatments and resins was high (p<0.0001 for both), indicating that the effect of the treatment applied to the specimen surface and the effect of the type of resin on surface roughness was highly significant. Regarding the interaction between polishing system and type of resin used, a p value of 0.0002 was obtained, indicating a statistically significant difference. A Ra of 1.3663 was obtained for the Sof-Lex/TPH Spectrum interaction. In contrast, the Ra for the felt disk+paste/Z250 interactions was 0.1846. In conclusion, Sof-Lex polishing system produced a higher surface roughness on TPH Spectrum resin when compared to the other interactions.