Maximizing the extraction yield of plant gum exudate using response surface methodology and artificial neural networking and pharmacological characterization.

Prunus armeniaca gum is used as food additive and ethno medicinal purpose. Two empirical models response surface methodology and artificial neural network were used to search for optimized extraction parameters for gum extraction. A four-factor design was implemented for optimization of extraction process for maximum yield which was obtained under the optimized extraction parameter (temperature, pH, extraction time, and gum/water ratio). Micro and macro-elemental composition of gum was determined by using laser induced breakdown spectroscopy. Gum was evaluated for toxicological effect and pharmacological properties. The maximum predicted yield obtained by response surface methodology and artificial neural network was 30.44 and 30.70% which was very close to maximum experimental yield 30.23%. Laser induced breakdown spectroscopic spectra confirmed the presence Calcium, Potassium, Magnesium, Sodium, Lithium, Carbon, Hydrogen, Nitrogen and Oxygen. Acute oral toxicity study showed that gum is non-toxic up to 2000 mg/Kg body weight in rabbits, accompanied by high cytotoxic effects of gum against HepG2 and MCF-7cells by MTT assay. Overall, Aqueous solution of gum showed various pharmacological activities with significant value of antioxidant, antibacterial, anti-nociceptive, anti-cancer, anti-inflammatory and thrombolytic activities. Thus, optimization of parameters using mathematical models cans offer better prediction and estimations with enhanced pharmacological properties of extracted components.

Deciphering host-pathogen interaction during Streptomyces spp. infestation of potato.

Potato crop, currently, is the staple food crop of about 1.3 billion global population. Potato is attaining even more admiration globally day by day owing to its public acceptability. However, potato sustainable production is distinctly challenged by multiple factors like diseases, pests and climate change etc. Among diseases, common scab is one of the prime threats to potato crop due to its soil-borne nature and versatility in phytotoxins' secretion. Common scab is caused multiple number of phytopathogenic streptomyces strains. Despite extensive research programs, researchers are still unable to identify a significant solution to this threat that is proliferating exceptional rate across the globe. To develop feasible remedies, adequate information regarding host-pathogen interaction should be available. This review possesses insights on existing pathogenic species, the evolution of novel pathogenic streptomyces spp. and phytotoxins produced by the pathogenic strains. Furthermore, which type of physiological, biochemical and genetic activities occur during pathogen's infestation of the host are also canvassed.

Effect of green and chemically synthesized titanium dioxide nanoparticles on cadmium accumulation in wheat grains and potential dietary health risk: A field investigation.

A field study was designed to explore the impacts of foliar-applied chemically and green synthesized titanium dioxide nanoparticles (TiO2 NPs) on cadmium (Cd) uptake in wheat plants. The wheat was grown in field which was contaminated with Cd and plants were subjected to foliar episodes of TiO2 NPs during plant growth period. Leaf extracts of two plant species (Trianthema portulacastrum, Chenopodium quinoa) were used for green synthesis while sol-gel method was used for chemical preparation of TiO2 NPs. Results showed that TiO2 NPs significantly enhanced the plant height, length of spikes photosynthesis, and straw and grain yield compared to control. TiO2 NPs minimized the oxidative burst in leaves and improved the enzyme activities than control. Cadmium concentrations of straw, roots and grains decreased after TiO2 NPs treatments than control. The grain Cd contents were below recommended threshold (0.2 mg Cd /kg grain DW) for cereals upon NPs exposure. The health risk index by the dietary use of grains for adults was below threshold upon NPs exposure. Overall, foliar use of TiO2 NPs prepared from plant extracts was appropriate in minimizing Cd contents in wheat grains, thereby reducing risk of Cd to human health via food chain.

Combined application of Bacillus sp. MN-54 and phosphorus improved growth and reduced lead uptake by maize in the lead-contaminated soil.

Lead (Pb) is considered an important environmental contaminant due to its considerable toxicity to living organisms. It can enter and accumulate in plant tissues and become part of the food chain. In the present study, individual and combined effects of Bacillus sp. MN-54 and phosphorus (P) on maize growth and physiology were evaluated in Pb-contaminated soil. A pristine soil was artificially contaminated with two levels of Pb (i.e., 250 and 500 mg kg-1 dry soil) and was transferred to plastic pots. Bacillus sp. MN-54 treated and untreated maize (DK-6714) seeds were planted in pots. Recommended doses of nutrients (N and K) were applied in each pot while P was applied in selective pots. Results showed that Pb stress hampered the maize growth and physiological attributes in a concentration-dependent manner, and significant reductions in seedling emergence, shoot and root lengths, fresh and dry biomasses, leaf area, chlorophyll content, rate of photosynthesis, and stomatal conductance were recorded compared with control. Application of Bacillus sp. MN-54 or P particularly in combination significantly reduced the toxic effects of Pb on maize. At higher Pb level (500 mg kg-1), the combined application effectively reduced Pb uptake up to 42.4% and 50% by shoots, 30.8% and 33.9% by roots, and 18.4% and 26.2% in available Pb content in soil after 45 days and 90 days, respectively compared with that of control. Moreover, the use of Bacillus sp. MN-54 significantly improved the P uptake by maize plants by 44.4% as compared with that of control. Our findings suggest that the combined use of Bacillus sp. MN-54 and P could be effective and helpful in improving plant growth and Pb immobilization in Pb-contaminated soil.

Characterization of Hydrocarbon-Degrading Bacteria in Constructed Wetland Microcosms Used to Treat Crude Oil Polluted Water.

Ten plant species were grown in constructed wetlands (CWs) to remediate water containing 2% (w/v) crude oil. The plant species with better growth and biomass production were Typha latifolia and Cyperus laevigatus, and they were significantly correlated (R2 = 0.91) with hydrocarbon degradation. From T. latifolia and C. laevigatus, 33 hydrocarbon-degrading bacterial strains were isolated from the rhizosphere, and root and shoot interiors. More diversified bacteria were found in the rhizosphere and endosphere of C. laevigatus than those of T. latifolia. The predominant cultural hydrocarbon-degrading bacteria were shown to belong to the genera Pseudomonas, Acinetobacter and Bacillus. In addition to genes involved in hydrocarbon degradation, most of the bacteria displayed multiple plant growth promoting (PGP) activities. This study suggests the importance of selecting suitable bacterial strains with hydrocarbon degradation and PGP activities for improving the efficacy of CWs used in remediating water contaminated with crude oil.