RESUMO
Objectives of this study were to determine effects of dietary true fecal digestible Ca to true digestible P ratio on growth performance and efficiency of Ca and P use in growing pigs fed corn (Triticum aestivum)-soybean (Glycine max) meal (SBM)-based diets. Experiment 1 was carried out to measure true fecal digestibility of Ca and P as well as the fecal endogenous outputs of these nutrients associated with a corn and SBM-based diet in 12 Yorkshire growing pigs with an average initial BW of 23.2 ± 0.6 kg by the substitution method. True fecal digestibility values (%; n = 6) of Ca (53.6 ± 12.7) and P (43.8 ± 16.7) as well as the fecal endogenous outputs (g/kg DMI; n = 12) of Ca (0.91 ± 0.20) and P (1.31 ± 0.15) associated with the diets were determined. Experiment 2 was conducted with 36 Yorkshire barrows of an average initial BW of 24.2 ± 0.6 kg and the pigs were fed 6 diets according to a completely randomized block design. The 6 diets were corn and SBM based with diet 1 containing 0.2% true digestible Ca and 0.3% true digestible P and were formulated to contain 6 total Ca to total P ratios based on analyzed dietary Ca and P contents (diet 1, 0.6:1; diet 2, 0.7:1; diet 3, 0.8:1; diet 4, 1.3:1; diet 5, 1.0:1; and diet 6, 1.3:1) by supplementing gradient levels of limestone with a constant dietary P content for meeting the recommended requirement. Changes in the dietary Ca to P ratio had no effects (P > 0.05) on ADG. No differences (P > 0.05) in ADFI were observed between the other diets except the lower ADFI (P < 0.05) in diet 3 compared with diet 2. However, G:F was higher (P < 0.05) in diet 2 compared with diets 5 and 6. Changes in the dietary Ca to P ratio had consistent effects on true fecal P digestibility and retention with much lower values (P < 0.05) observed in diet 5 in comparison with the other diets. In summary, true fecal digestible Ca to P ratios of 0.9:1 to 1.0:1 were associated with optimal responses in both G:F as well as true fecal P digestibility and retention in growing pigs fed corn and SBM-based diets.
Assuntos
Ração Animal/análise , Cálcio/metabolismo , Glycine max , Fósforo/metabolismo , Suínos/crescimento & desenvolvimento , Zea mays , Fenômenos Fisiológicos da Nutrição Animal , Animais , Cálcio/química , Cálcio da Dieta/análise , Cálcio da Dieta/metabolismo , Dieta/veterinária , Masculino , Fósforo/química , Fósforo na Dieta/análise , Fósforo na Dieta/metabolismo , Suínos/metabolismoRESUMO
Whereas dietary fibers are well recognized for nutritional management of human health issues, fiber is also known to be one of the dietary factors potentially affecting digestive use of dietary proteins. As a staple food, potato (Solanum tuberosum) may be a significant dietary fiber source. The objective of this study was to examine effects of dietary supplementation of six potato cultivar-genotype samples that differ in soluble fiber content and two conventional fiber components (i.e., cellulose and guar gum) on the apparent ileal AA digestibility in pigs fed a high-fat basal diet. The basal diet was formulated as a zero-fiber negative control (NC) to contain 41.5% poultry meal, 4% casein, 15% animal fat-oil blend, 2.8% sucrose, 31% corn (Zea mays) starch, 0.50% salt, and 0.40% trace mineral-vitamin supplement with fat contributing to 47% of the dietary GE. The two fiber diets were formulated by respectively diluting the basal diet with 10% guar gum and 10% cellulose at the expense of corn starch. Six other test diets were formulated by including 8.5% guar gum and further diluting the basal diet with 25.1% one of the six cultivar-genotype samples of dehydrated potato tuber powder to contain about 10% total dietary fiber at the expense of corn starch. Eighty-one 25-kg barrows were fitted with a simple T-cannula at the distal ileum and fed the diets according to a completely randomized block design with each block lasting 28 d. Compared with the NC, the ileal digestibility of Ala, Gly, and Pro were decreased (P < 0.05) by 10% guar gum whereas the digestibility of Gly was reduced (P < 0.05) by 10% cellulose. The ileal digestibility of several AA was decreased (P < 0.05) by the test potatoes plus 8.5% guar gum compared with the NC. Our results suggest that dietary inclusion of fiber at 10% from guar gum and cellulose and contributed by potatoes may adversely affect digestive use of dietary protein.
Assuntos
Aminoácidos/metabolismo , Fibras na Dieta/metabolismo , Digestão/fisiologia , Íleo/fisiologia , Solanum tuberosum/química , Suínos/fisiologia , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Gorduras na Dieta/administração & dosagem , Gorduras na Dieta/metabolismo , Gorduras na Dieta/farmacologia , Fibras na Dieta/análise , MasculinoRESUMO
The porcine gut microbiome is a novel genomic resource for screening cellulose-degrading enzymes. A plasmid metagenomic expression library was constructed from the hindgut microbiota of 6 Yorkshire growing pigs (25 to 40 kg) fed a high-fat basal diet supplemented with 10% Solka-Floc for 28 d. Fresh cecal and colonic digesta samples were collected, flash-frozen in liquid N, and stored under -80°C. Metagenomic DNA was extracted, mechanically sheared, and cleaned to remove small DNA fragments (<1.0 kb). The resulting DNA fragments were subjected to blunt-end polishing, fractionation, and purification by using commercial kits. The end-modified DNA fragments were ligated to pCR4Blunt-TOPO vector and transformed into competent Escherichia coli TOPO10 cells. Metagenomic plasmid libraries were screened for carboxymethyl cellulolytic activities by using lysogeny broth agar plates. The average insert size of the resulting library was approximately 4.2 kb. Screening for the ability to hydrolyze carboxymethyl cellulose yielded 14 positive colonies, giving an estimated 430 Mb of metagenomic DNA in the approximately 102,000 E. coli clones with an overall hit rate of 0.14%. The 11 assembled insert sequences included 4 function-related gene clusters, and a total of 18 putative carbohydrate active enzyme genes were identified. This included genes encoding 11 cellulases, 4 hemicellulases, 1 polygalacturonas, 1 glycoside hydrolase family 26 mannanase-family 5 cellulase chimeric enzyme gene, and 1 cellobiose phosphorylase. In conclusion, the coupling of functional metagenomic mining with biochemical characterization of fiber-degrading enzymes is a powerful strategy for exploring the enzymological underpinnings of the anaerobic fermentation of dietary fiber in the complex animal gut environment.
Assuntos
Ração Animal/análise , Bactérias/genética , Celulases/metabolismo , Celulose/química , Biblioteca Genômica , Suínos/fisiologia , Fenômenos Fisiológicos da Nutrição Animal , Animais , Bactérias/classificação , Celulases/genética , Celulose/metabolismo , Dieta/veterinária , Gorduras na Dieta/administração & dosagem , Suplementos Nutricionais , Relação Dose-Resposta a Droga , Conteúdo Gastrointestinal/microbiologia , Regulação Bacteriana da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Genoma BacterianoRESUMO
BACKGROUND: Inflammatory bowel disease (IBD), a chronic inflammation of the gastrointestinal tract, is characterized by a deregulation of the mucosal immune system and resistance of activated T cells to apoptosis. Current therapeutics show limited efficacy and potential toxicity; therefore there is a need for novel approaches for the treatment of IBD. L-cysteine was examined for its ability to reduce colitis symptoms and modulate local gene expression in a DSS-induced porcine model of colitis. METHODS: Colitis was induced via intra-gastric infusion of dextran sodium sulfate (DSS), followed by the administration of L-cysteine or saline. Clinical signs, morphological measurements, histology and gut permeability were assessed for the prognosis of colitis. Local tissue production of cytokines and gene expression in the colon were analyzed by ELISA and real-time RT-PCR. RESULTS: L-cysteine supplementation attenuated DSS-induced weight loss and intestinal permeability, reduced local chemokine expression and neutrophil influx, and markedly improved colon histology. Furthermore, cysteine significantly reduced the expression of pro-inflammatory cytokines, including TNF-alpha, IL-6, IL-12p40, IL-1beta, and resulted in increased expression of the apoptosis initiator caspase-8 and decreased expression of the pro-survival genes cFLIP and Bcl-xL. CONCLUSIONS AND GENERAL SIGNIFICANCE: These results suggest that L-cysteine administration aids in restoring gut immune homeostasis by attenuating inflammatory responses and restoring susceptibility of activated immune cells to apoptosis, and that cysteine supplementation may be a novel therapeutic strategy for the treatment of IBD.
Assuntos
Colite/prevenção & controle , Cisteína/administração & dosagem , Trato Gastrointestinal/efeitos dos fármacos , Homeostase/efeitos dos fármacos , Inflamação/prevenção & controle , Animais , Animais Recém-Nascidos , Caspase 8/genética , Colite/induzido quimicamente , Colo/efeitos dos fármacos , Colo/metabolismo , Colo/patologia , Sulfato de Dextrana , Suplementos Nutricionais , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Trato Gastrointestinal/metabolismo , Trato Gastrointestinal/patologia , Expressão Gênica/efeitos dos fármacos , Subunidade p40 da Interleucina-12/genética , Subunidade p40 da Interleucina-12/metabolismo , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Interleucina-6/genética , Interleucina-6/metabolismo , Absorção Intestinal/efeitos dos fármacos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Suínos , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo , Proteína bcl-X/genéticaRESUMO
The objectives of this study were to determine true P digestibility, the gastrointestinal endogenous P outputs associated with soybean meal (SBM), and the role of the large intestine in P digestion in growing pigs. Four Yorkshire barrows, with average initial and final BW of 40 and 58 kg, were fitted with a simple T-cannula at the distal ileum and fed four diets according to a 4 x 4 Latin square design. The diets were cornstarch-based and contained four levels of P (0.098, 0.196, 0.293, and 0.391% on a DM basis) from solvent-extracted conventional SBM. Chromic oxide (3.5 g/kg of diet, as-fed basis) was included as a digestibility marker. Each experimental period consisted of 8 d with a 4-d adaptation period and a 4-d collection of representative ileal digesta (2 d) and fecal (2 d) samples. True ileal and fecal P digestibility values and the ileal and fecal endogenous P outputs associated with SBM were determined by the regression analysis technique. There were no differences (P > 0.05) in true P digestibility values (ileal, 59.0 +/- 8.3 vs. fecal, 51.3 +/- 7.9%, n = 16) and endogenous P outputs (ileal, 0.59 +/- 0.18 vs. fecal, 0.45 +/- 0.21 g/kg of DMI, n = 16) between the ileal and the fecal levels. The endogenous fecal P loss accounted for 8.1 and 17.6% of the NRC (1998) recommended total and available P requirements in growing pigs, respectively. In conclusion, approximately 51% of the total P in conventional SBM is digested in growing pigs. The large intestine does not play an important role in the digestion of P associated with SBM in the growing pig. The fecal loss of the gastrointestinal endogenous P is an important route of P excretion in the growing pig.
Assuntos
Digestão , Glycine max , Íleo/metabolismo , Fósforo/metabolismo , Suínos/metabolismo , Animais , Compostos de Cromo/metabolismo , Relação Dose-Resposta a Droga , Fezes/química , Absorção Intestinal , Masculino , Necessidades Nutricionais , Valor Nutritivo , Distribuição Aleatória , Análise de Regressão , Suínos/crescimento & desenvolvimentoRESUMO
Methodology was developed for measuring the gastrointestinal endogenous phosphorus (P) outputs and true P digestibility values in studies with piglets. Four barrows, average initial body weight 6.8 kg, were fitted with a simple T-cannula at the distal ileum and fed four diets according to a 4 x 4 Latin square design. Four cornstarch-based diets containing four levels of P (1.1, 2.1, 3.2 and 4.3 g/kg diet) on a dry matter (DM) basis were formulated from soybean meal (SBM). Each experimental period comprised 8 d with a 4-d adaptation and 4-d collection of ileal digesta and feces. The apparent ileal and fecal P digestibility values in SBM were affected (P < 0.05) by P levels in the assay diets. The ileal and fecal P digestibility values increased from -24.8 to 37.1% and from 18.8 to 42.5%, respectively, as P contents increased from 1.1 to 4.3 g/kg DM diet. Linear relationships (P < 0.05), expressed as g/kg DM diet intake, between ileal and fecal outputs and dietary inputs of P, suggested that the endogenous P outputs can be determined by linear regression analysis. The endogenous P output was higher (P < 0.05) in ileal digesta than in feces (0.86 +/- 0.09 vs. 0.31 +/- 0.06 g/kg DM diet intake). There was no difference (P > 0.05) between the true ileal (50.7 +/- 7.1%) and fecal (48.5 +/- 5.4%) P digestibility values in SBM. These results suggest that differences in P contents between assay diets are primarily responsible for the large variability in apparent P digestibility values reported within the same ingredient. Apparent digestibility values underestimate the true digestive utilization of P by approximately 25%. True rather than apparent P digestibility values should be determined and used in diet formulation for pigs. In addition, this study shows that the gastrointestinal endogenous P output is important in whole-body P requirement and homeostasis.