Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
J Immunol Methods ; 468: 1-9, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30802448

RESUMO

The presence of background autofluorescence sources is considered as an important problem when performing fluorometric methods, due to the possible spectral overlap between it and the fluorescence emission of probes. Regarding that, we evaluated the presence of background autofluorescence in human lymphocytes after the treatment with extracts from three medicinal plants, including ethanolic extract from aerial parts of Ageratum fastigiatum, ethanolic extract from aerial parts of Eriosema campestre and the ethanolic extract from stem of Pseudobrickellia brasiliensis. Human peripheral blood mononuclear cells were treated with each extract in vitro during 24 h, followed by flow cytometric analysis. Additionally, the fluorescence emission of plant extracts was evaluated by fluorometry, using the same concentrations used in cell cultures. We identified that plant extracts treatment on lymphocytes induced background autofluorescence detectable in several wavelength ranges. Isolated extracts showed no expressive fluorescence emission in fluorometric analyses, suggesting that background autofluorescence was induced in lymphocytes by interactions between cellular components and extracts compounds. Here we discuss the importance to perform previous tests to evaluate a possible background autofluorescence induction after cell treatments with plant extracts or any other substance. In spite of being mandatory, background autofluorescence analysis of cells after treatments and stimulations is still underestimated on literature. In summary, following the precautions herein established should help to reduce the incidence of false positive results.


Assuntos
Citometria de Fluxo , Linfócitos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Adulto , Ageratum , Asteraceae , Células Cultivadas , Fabaceae , Reações Falso-Positivas , Humanos , Medições Luminescentes , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Reprodutibilidade dos Testes
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA