RESUMO
Goats were infected experimentally with a mycoplasma (the "Irbid" strain) isolated previously from a goat with contagious agalactia in northern Jordan. The strain was unusual in that, although it had been identified by molecular methods as Mycoplasma mycoides subsp. mycoides LC/Mycoplasma mycoides subsp. capri, it showed no inhibition of growth by any of the hyperimmune rabbit antisera conventionally used to speciate members of the Mycoplasma mycoides cluster. Animals were infected either intratracheally or by aerosol and placed "in-contact" with other goats. After 2 weeks, those infected intratracheally became febrile, showing a nasal discharge and slight conjunctivitis, followed a week later by respiratory distress and polyarthritis; lesions seen at necropsy included coagulative necrotic pneumonia, fibrinous pleurisy with pleural exudate, and inflammatory exudates, necrosis and fibrosis in the joints. Animals infected by aerosol showed much milder clinical signs, including nasal discharge and occasional swollen joints. In the "in-contact" goats, seroconversion was first seen after 7 weeks, accompanied by coughing and laboured respiration; lesions in this group consisted of fibrinous pneumonia with focal areas of necrosis and abundant pleural exudate.
Assuntos
Doenças das Cabras/microbiologia , Mycoplasma mycoides/patogenicidade , Pleuropneumonia Contagiosa/microbiologia , Animais , Artrite/microbiologia , Artrite/patologia , Artrite/veterinária , Conjuntivite/microbiologia , Conjuntivite/patologia , Conjuntivite/veterinária , Febre/microbiologia , Febre/patologia , Febre/veterinária , Fibrose/microbiologia , Fibrose/patologia , Doenças das Cabras/patologia , Doenças das Cabras/transmissão , Cabras , Articulações/microbiologia , Articulações/patologia , Mycoplasma mycoides/fisiologia , Necrose/microbiologia , Necrose/patologia , Pleuropneumonia Contagiosa/patologia , Pleuropneumonia Contagiosa/transmissão , CoelhosRESUMO
The in vitro effect of six Jordanian traditional medicine plant methanolic extracts were tested against 32 isolates of Mycoplasma species; Mycoplasma mycoides subsp. mycoides LC (6), Mycoplasma capricolum subsp. capricolum (8) and M. putrefaciens (18), all isolated from either nasal swabs or milk, from sheep and goats in different regions in Jordan. All Mycoplasma species showed susceptibility to Artemisia herba-alba and Artemisia arborescens with MIC ranges from 3.125-12.5 mg/ml. Allium sativum and Punica grantum showed limited activity against some Mycoplasma isolates. Olea europea and Citrullus colocynthis showed no in vitro activity against any of the Mycoplasma species tested. Artemisia herba-alba and Artemisia arborescens may therefore be useful for the treatment of mycoplasma infections.