RESUMO
This study focused on the biological evaluation and chemical characterization of Malabaila lasiocarpa Boiss. (M. lasiocarpa) (Family: Apiaceae). The phytochemical profile, antioxidant, enzyme inhibitory of the methanolic, aqueous, dichloromethane, hexane extracts were investigated. Based on UHPLC-HRMS analyses, a total of 101 peaks were annotated or identified for the first time in M. lasiocarpa extracts. They include hydroxybenzoic, hydroxycinnamic, acylquinic acids and their glycosides, C- and O-glycosyl and O-diglycosyl flavonoids. In addition, 10 simple mono- and disubstituted coumarins together with 10 furanocoumarins were tentatively annotated. The methanolic extract possessing the highest phenolic (24.36±0.60â mg gallic acid equivalent/g extract) and flavonoid (69.15±0.37â mg rutin equivalent/g extract) content also exhibited the strongest radical scavenging potential against 2,2-diphenyl-1 picrylhydrazyl (21.73±0.42â mg Trolox equivalent/g extract, respectively), and highest reducing capacity (57.81±0.97 and 28.00±0.40â mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively). The dichloromethane extract substantially depressed the tyrosinase (73.92±5.37â mg kojic acid equivalent/g extract), α-amylase (0.63±0.01â mmol acarbose equivalent/g extract) and α-glucosidase (0.69±0.02â mmol acarbose equivalent/g extract) enzymes. This study has produced critical scientific data on M. lasiocarpa which are potential contenders for the development of novel phyto-pharmaceuticals.
Assuntos
Antioxidantes , Apiaceae , Acarbose , Antioxidantes/química , Antioxidantes/farmacologia , Flavonoides/análise , Cloreto de Metileno/análise , Extratos Vegetais/química , TurquiaRESUMO
The aim of the present study was to quantify selected phenolic compounds, determine antioxidant activity and enzyme inhibitory effects of the aerial parts of Alkanna trichophylla Hub.-Mor. (A.â trichophylla) and Convolvulus galaticus Rost.ex Choisy (C.â galaticus) extracts prepared by homogenizer-assisted extraction (HAE), maceration (MAC) and infusion techniques. This is the first time such study has been designed to validate the phytochemical composition and bioactivity of these plants. Multivariate analysis was conducted on collected data. Rutin and caffeoylquinic acid derivatives were the most significant compounds in A.â trichophylla and C.â galaticus, respectively. The highest antioxidant activity of A.â trichophylla was mostly exhibited by HAE/methanolic extracts as determined by DPPH, ABTS, FRAP (51.39, 112.70 and 145.73â mg TE/g, respectively) and phosphomolybdenum (2.05â mmol TE/g) assays. However, significant antioxidant activities varied within the extracts of C.â galaticus. HAE/methanolic extract of A.â trichophylla significantly depressed AChE (2.70â mg GALAE/g), BChE (5.53â mg GALAE/g) and tyrosinase (26.34â mg KAE/g) activities and that of C.â galaticus inhibited AChE (2.04â mg GALAE/g), tyrosinase (31.25â mg KAE/g) and α-amylase (0.53â mmol ACAE/g) activities significantly. We concluded that HAE was the most efficient extraction technique as high yield of compounds and promising bioactivities were recorded from extracts prepared. Multivariate analysis showed that types of solvents influenced recovery of compounds and biological activities. This research study can be used as one methodological starting point for further investigation on these plants as all results are clearly promising and open the door to further research challenges such as cytotoxicity evaluation, molecular docking analysis, and more screening of pharmacological actions.