RESUMO
The aqueous extracts of Citrus unshiu peel containing flavonoid glycosides was used as co-substrate with Schizophyllum commune mycelia producing ß-glucosidase and its biological activities were studied. ß-glucosidase-produced S. commune mycelia converted the glycosides (narirutin and hesperidin) into aglycones (naringenin and hesperetin). The photoprotective potential of fermented C. unshiu peel extract with S. commune (S-CPE) was tested in human dermal fibroblasts (HDFs) exposed to UVA. It was revealed that S-CPE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with S-CPE resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA. The UVA irradiation raised the proportion of senescence-associated ß-galactosidase (SA-ß-gal) positive cells in comparison with the normal control group. The treatment of UVA-irradiated HDFs with S-CPE was shown to decrease the level of SA-ß-gal (by approximately 45% at an S-CPE concentration 0.1%, w/v) compared with the UVA-irradiated HDFs. It was found that S-CPE containing hesperetin has notable collagen biosynthetic activity for fibroblasts, indicating that S-CPE can be promising cosmetic ingredients.
Assuntos
Citrus/química , Derme/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/efeitos da radiação , Extratos Vegetais/farmacologia , Células Cultivadas , Colágeno/biossíntese , Derme/efeitos da radiação , Dissacarídeos/metabolismo , Fermentação , Flavanonas/metabolismo , Frutas/química , Hesperidina/metabolismo , Humanos , Metaloproteinase 1 da Matriz/metabolismo , Schizophyllum/crescimento & desenvolvimento , Schizophyllum/metabolismo , Envelhecimento da Pele/efeitos dos fármacos , Raios Ultravioleta , beta-Galactosidase/metabolismoRESUMO
Fructus arctii extract containing phenolic glycosides was cultured with Grifola frondosa mycelia to produce ß-glucosidase and its biological activities were studied. This ß-glucosidase converted the glycosides (arctiin and caffeic acid derivatives) into aglycones (arctigenin and caffeic acid). Fermented Fructus arctii extract (G-FAE) with G. frondosa had antioxidant and 5-lipoxygenase inhibitory activities. The photoprotective potential of G-FAE was tested in human dermal fibroblasts (HDF) exposed to ultra-violet A (UVA). It was revealed that G-FAE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDF. The treatment of UVA-irradiated HDF with G-FAE resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA. G-FAE also showed notable stimulation of collagen biosynthetic activity for fibroblasts. These diverse functionalities suggest that G-FAE could be a promising cosmetic ingredient.
Assuntos
Antioxidantes/farmacologia , Arctium/metabolismo , Cosméticos/química , Grifola/metabolismo , Inibidores de Lipoxigenase/farmacologia , Extratos Vegetais/farmacologia , beta-Glucosidase/metabolismo , Antioxidantes/química , Antioxidantes/metabolismo , Células Cultivadas , Derme/efeitos dos fármacos , Fermentação , Fibroblastos/efeitos dos fármacos , Grifola/química , Grifola/genética , Humanos , Lipoxigenase/metabolismo , Inibidores de Lipoxigenase/química , Metaloproteinase 1 da Matriz/metabolismo , Inibidores de Metaloproteinases de Matriz , Micélio/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Raios UltravioletaRESUMO
Superoxide radical scavenging activity and DPPH radical scavenging activity were assessed in order to evaluate the antioxidant effect of the Sorbus commixta Hedl. extract (SCoE). SCoE was also treated with several carbohydrate-hydrolytic enzymes that significantly increased the total phenol and flavonoid composition of SCoE. The enzymatically treated SCoE was then assessed for antioxidative activity. The most efficient radical scavenging activity was observed when SCoE was treated with -glucanase. The radical scavenging activity of beta-glucanase-treated SCoE (beta-GSCoE) enhanced the viability of human dermal fibroblasts (HDFs) exposed to ultraviolet (UV) light. The intracellular reactive oxygen species (ROS) scavenging activity of beta-GSCoE was assessed using UVB (20 mJ/cm2)-irradiated HDFs. UVB irradiation increased dichlorofluorescein (DCF) fluorescence, which was measured by a 5-(6-)chloromethyl-2',7'- dichlorodihydrofluorescein diacetate (CM-H2DCFDA). DCF-fluorescence was significantly decreased in the beta-GSCoE-containing culture medium, suggesting that beta-GSCoE scavenges free radicals. The protective effect was further verified by assessing the expression of matrix metalloproteinase-1 (MMP-1) in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with beta-GSCoE resulted in a dose-dependent decrease in the expression level of MMP-1 protein and mRNA. These results suggest that beta-GSCoE may mitigate the effects of photoaging in skin by reducing UV-induced adverse skin reactions.