Study on the hematological toxicity of Cyprinus carpio exposed to water-soluble fraction of crude oil and TiO2 nanoparticles in the dark and ultraviolet.

This study is addressing the potential toxicity concerns of crude oil in common carp (Cyprinus carpio) with the novel use of TiO2-NP for enhancing photocatalytic degradation of WSF of crude oil prepared under UV light or darkness. Blood samples were taken, and the biochemical parameters were analyzed. The levels of ALT, AST and ALP were significantly higher in fish exposed to UV-treated WSF. However, they were significantly lower in the groups exposed to UV-treated TiO2-NPs and the combination of WSF and TiO2-NPs. The levels of total protein, triglycerides, albumin and cholesterol were significantly lower in treatments exposed to UV-treated and dark-conditioned WSF compared to the control group, but they were significantly higher in fish exposed to UV-treated TiO2-NPs and the combination of WSF and TiO2-NPs compared to fish exposed under dark conditions and were not significantly different from the control group. The toxicity of UV-treated WSF was significantly higher than that of dark-conditioned WSF. The toxicity of TiO2-NPs was lower in the presence of UV and was similar to the control treatment. The results of the study suggests that photocatalytic TiO2-NPs and UV radiation reduce toxicity of the water-soluble fraction of crude oil on common carp.

Investigation of Different Nutritional Effects of Dietary Chromium in Fish: A Literature Review.

The supply of food for the world population that is increasing is one of the concerns of governments. The Food and Agriculture Organization of the United Nations assessment shows that the aquaculture industry could help meet food needs for human communities. The aquaculture industry also relies on providing a feed of high quality. Minerals are one essential component of an aquatic diet. Chromium (Cr) is a trace element that finds the form of Cr+3 (trivalent) and Cr+6 (hexavalent) in nature and food items. Studies show that exposure to Cr waterborne have toxicity effects on fish. However, oral exposure to Cr has a different impact on fish. Cr is usually involved in the metabolism of fats, carbohydrates, proteins, growth function, enzyme functions, etc. This element could play a significant role in fish nutrition and physiology. Cr as a dietary supplement can improve growth performance and adjust the metabolism of carbohydrates and lipids. However, high concentrations of Cr can be toxic to fish. Although the physiological effects of Cr on aquatic organisms are well known, there are still ambiguities in determining the appropriate concentration in the diet of some species. Maybe, the physiological response of fish depends on the concentration, origin, and chemical composition of Cr, as well as the biological and individual characteristics of the fish. Therefore, it is necessary to estimate the appropriate concentration of Cr in fish diets. This article aims to summarize the available information about the effect of Cr on various physiological indicators and fish growth. Therefore, this information may help to find the appropriate concentration of Cr in the diet.

Protective effect of protexin concentrate in reducing the toxicity of chlorpyrifos in common carp (Cyprinus carpio).

The present study aimed to evaluate the protective effect of protexin supplementation against chlorpyrifos-induced oxidative stress and immunotoxicity in Cyprinus carpio. After 21 days, the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR), and total antioxidant levels significantly decreased in hepatocytes of fish exposed to chlorpyrifos, while malondialdehyde (MDA) increased. Treatment with protexin was able to reverse the decrease in SOD and GR and significantly reduce MDA levels. Exposure to chlorpyrifos also induced alterations in blood biochemical parameters and caused immunosuppression. Dietary protexin return some parameters (aspartate aminotransferase, lactate dehydrogenase, and γ-glutamyltransferase activities, and glucose, cholesterol, total protein, creatinine, and complement C4 levels) to values similar to those of the control group. Based on the results, it can be concluded that protexin exerted protective effects against chlorpyrifos exposure in C. carpio reducing oxidative damage, and ameriorating blood biochemical alterations and the immunosuppression.

Effect of petroleum wastewater treated with gravity separation and magnetite nanoparticles adsorption methods on the blood biochemical response of mrigal fish (Cirrhinus cirrhosus).

Drainage of treated wastewater to surface water is a severe threat to the health of aquatic organisms. This study aimed to evaluate the effects of 0.5 and 1% water-soluble fractions of crude oil (WSFO), WSFO treated with magnetic nanoparticles of Fe3O4 (TWSFO-Fe3O4) and with the gravity separation method (TWSFO-GSM) on Cirrhinus cirrhosis for 21 days. The rate of erythrocyte hemolysis in fish exposed to untreated 0.5 and 1% WSFO were significantly high. The activities of alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP) were significantly increased in the groups exposed to TWSFO-GSM compared to the control group, while lactate dehydrogenase (LDH) was reduced. No significant differences in LDH, ALT, ALP, and GGT activities were observed in the fish treated with TWSFO-Fe3O4. The aspartate aminotransferase activity was significantly increased after exposure to TWSFO-Fe3O4 (1%) and TWSFO-GSM. The levels of triglyceride were decreased, whereas glucose, cholesterol, and cholinesterase activity increased in fish after both treatments. The total protein and albumin contents significantly decreased in fish under exposure to both doses of TWSFO-Fe3O4 and TWSFO-GSM. The globulin level decreased in fish exposed to TWSFO-Fe3O4 (1%) and TWSFO-GSM. Glutathione peroxidase, catalase, glucose-6-phosphate dehydrogenase activities, and total antioxidant levels were significantly reduced in the hepatocytes of fish exposed to TWSFO-Fe3O4, TWSFO-GSM, and WSFO, while superoxide dismutase activity and malondialdehyde content were increased. This study showed that despite removing oil drips from the WSFO, the xenobiotics present in the effluent treated by gravitational or nano-magnetite methods caused changes in biochemical parameters and induced oxidative stress. Therefore, it is recommended to prevent the discharge of treated effluent from the oil and petrochemical industries to aquatic ecosystems.

Genotoxicity, oxidative stress, and biochemical biomarkers of exposure to green synthesized cadmium nanoparticles in Oreochromis niloticus (L.).

The considerable increment in the use of Nanoparticles in the industry has been recognized as an environmental concern today. Therefore, this study aimed to investigate the toxicity effects of green synthesized cadmium nanoparticles [Cd]NPs using Moringa oleifera leaves extract on multi-biomarkers in Oreochromis niloticus after four weeks of exposure. The results showed that LC50 values of [Cd]NPs for 24, 48, 72 and 96 h were 2.17, 1.75, 1.49 and 1.22 mg l-1, respectively. There was a significant decrease in the number of white and red blood cells, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration value in fish exposed to [Cd]NPs. The mean corpuscular volume and neutrophils were increased. [Cd]NPs exposure to fish has led to cytotoxic and genotoxic changes in the erythrocytes. Significant changes were observed in the cortisol, triiodothyronine, and thyroxine levels of the fish exposed to [Cd]NPs. The activities of aspartate aminotransferase and alanine aminotransferase increased. Glucose, total lipids, urea, and creatinine levels increased in the serum of fish exposed to [Cd]NPs, whereas total protein contents and alkaline phosphatase activity decreased. A significant reduction was observed in glycogen, total antioxidant levels, and superoxide dismutase, catalase and glutathione S-transferase activities of fish exposed to [Cd]NPs. In contrast, the [Cd]NPs exposure resulted in a significant increase in DNA fragmentation percentages, lipid peroxidation, and carbonyl protein levels in different tissues. The results of the present study confirmed that [Cd]NPs has the toxicity potential to cause Cyto-genotoxicity, oxidative damages, changes in the hematological and biochemical changes, and endocrine disruptor in the fish.

Selenium protection against mercury toxicity on the male reproductive system of Clarias gariepinus.

The present study aimed to evaluate the protective role of Selenium (Se) (0.1 ppm) on the male reproductive system of the catfish Clarias gariepinus exposed to sublethal doses of Mercury (Hg) (0.04 and 0.12 ppm) for 30 days. Indicators of seminal and gonadal hormone disruption (testosterone, estradiol and 11 keto testosterone), antioxidants (total antioxidant capacity (TAO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)), oxidative stress biomarkers (lipid peroxidation (LPO), percentage of DNA fragmentation, carbonylated proteins (CP) and nitric oxide (NO)) and histopathological alterations in testicles of Clarias gariepinus were determined. The exposure to Hg resulted in a high accumulation of residues of this metal in testicular tissues. The results showed a significant decrease in sperm count, activity and motility and in all gonadal hormones in Hg exposed groups. Hg exposure also induced a decline in TAO, SOD, CAT and GPx, whereas LPO, DNA fragmentation, CP and NO significantly increased in testicles of C. gariepinus respect to the control group. Although exposure to Se did not reduce the degree of mercury bioconcentration in the testicles, the sperm quality parameters were recovered. Moreover, TAO levels and GPx activity significantly increased after fish exposure to Se, whereas CP levels decreased. LPO, NO, CAT and SOD were also partially normalized when compared with the groups exposed to only Hg. In conclusion, the results showed that Hg, even in the small doses is capable to induce reproductive toxicity in the male catfish. Se exposure partially restored the values of biochemical parameters and sperm quality in Hg-treated fish suggesting protective effects against Hg reproductive toxicity.

Therapeutic effects of marshmallow (Althaea officinalis L.) extract on plasma biochemical parameters of common carp infected with Aeromonas hydrophila.

This study evaluated preclinical and clinical safety of marshmallow (Althaea officinalis L.) extract as a naturopathic medicine in common carp deliberately infected with Aeromonas hydrophila. The fish were fed 0 (control), 2.50, 5.00 and 10.00 g of marshmallow extract for 60 days in a preclinical experiment and then, challenged with A. hydrophila for a 10-day experiment. Significant increases were observed in aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), creatine phosphokinase (CPK) activities and plasma creatinine levels in fish fed 10 g marshmallow extract per kg feed. However, alanine aminotransferase (ALT) significantly decreased on day 60. The fish fed 2.50 g marshmallow extract per kg feed indicated increased levels of total protein and globulin. There were no significant changes in albumin levels (p > 0.05). 2.50 and 5.00 g marshmallow significantly decreased triglyceride and cholesterol levels and increased glucose levels (p < 0.05). A. hydrophila significantly increased AST, ALT, LDH, ALP and CPK activities and plasma glucose, cholesterol, triglycerides and creatinine levels after 10 days (p < 0.05). Total plasma protein, albumin and globulin levels in fish challenged with A. hydrophila were significantly lower than the control group (p < 0.05). Marshmallow extract at 5.00 and 10.00 g can adjust plasma biochemical parameters in fish challenged with A. hydrophila. The results of preclinical studies and pharmaceutical toxicity of marshmallow extract revealed that dietary levels lower than 5.00 g were safe and effective. The results of this clinical study demonstrated that marshmallow extract (5.00 g kg-1 feed) can protect fish against A. hydrophila.

Effects of long-term silymarin oral supplementation on the blood biochemical profile of rainbow trout (Oncorhynchus mykiss).

Silymarin, an extract from "milk thistle" (Silybum marianum) plant is traditionally used as herbal medicine. The present study was conducted to investigate the clinical effects and possible side effects of silymarin on biochemical blood parameters of rainbow trout (Oncorhynchus mykiss). Fishes were treated with 0 (control), 100, 400, and 800 mg of silymarin per kg of food during 4 weeks. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), creatine kinase (CK), glucose, total protein, creatinine, triglyceride, cholesterol, urea, uric acid and liver cellular total antioxidant, and protein content were measured after 7, 14, and 28 days of silymarin treatment. The results showed that oral administration of silymarin in fish significantly reduced plasma glucose and cholesterol levels and relatively increased plasma total protein and globulin concentrations (P < 0.05). Increasing plasma albumin levels indicate the important role of albumin in drug transportation in circulatory system of fish. Silymarin also stabilized cellular membrane structure and regulated the levels of AST, ALT, ALP, CK, and LDH activity. In conclusion, on the basis of these results, oral administration of silymarin up to 400 mg per 1 kg of food has no side effect on blood biochemical and clinical parameters of fishes. However, oral administration of 800 mg/kg- of silymarin caused cytotoxicity and modifications in blood biochemical parameters of fish.