Silicon induces phytochelatin and ROS scavengers facilitating cadmium detoxification in rice.

Cadmium (Cd) is detrimental to crops and the environment. This work examines the natural mechanisms underlying silicon- (Si-)directed Cd detoxification in rice plants. The addition of Si to plants under Cd stress caused significant improvements in morphological parameters, chlorophyll score, Fv /Fm and total soluble protein concentration compared to controls, confirming that Si is able to ameliorate Cd-induced damage in rice plants. This morpho-physiological evidence was correlated with decreased cell death and electrolyte leakage after Si application. The results showed no critical changes in root Cd concentration, while shoot Cd decreased significantly after Si supplementation in comparison with Cd-stressed rice. Additionally, expression of Cd transporters (OsNRAMP5 and OsHMA2) was significantly down-regulated while the concentration of phytochelatin, cysteine and glutathione, together with expression of OsPCS1 (phytochelatin synthase) in roots of Cd-stressed rice was significantly induced when subjected to Si treatment. This confirms that the alleviation of Cd stress is not only limited to the down-regulation of Cd transporters but also closely related to the phytochelatin-driven vacuolar storage of Cd in rice roots. The enzymatic analysis further revealed the role of SOD and GR enzymes in protecting rice plants from Cd-induced oxidative harm. These findings suggest a mechanistic basis in rice plants for Si-mediated mitigation of Cd stress.

Beliefs and treatment related to diarrhoeal episodes reported in association with measles.

Eighty mothers whose children had measles in the past month were interviewed for beliefs and practices related to the management of measles and measles associated diarrhoea. Beliefs and practices about diarrhoea, associated with measles are described. Diarrhoea during and after measles was considered beneficial by mothers, who believed it helped to flush out impurities from the body. Paradoxically ORS was not used because of an erroneous idea that ORS would stop diarrhoea. Informing mothers that ORS will not stop diarrhoea, but will help in flushing out the impurities, could enhance ORS use, reducing morbidity and mortality. The prevalent belief, that measles patients must be kept in a clean environment is useful and should be encouraged. Other beliefs and practices to hasten the eruption are neutral, but since they encourage cleanliness and isolation, need not be discouraged.