RESUMO
Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter describes a chromium (51Cr)-release bioassay designed to measure to the target cell killing capacity of NK cells (NKCC). Key features of the cytotoxicity assay are that it is done with whole blood and that numbers of effector cells are determined for each sample by flow cytometry and lymphocyte count. Effector cells are defined as CD3-CD56+ lymphocytes. Target cells are the K562 erythroleukemia cell line. Killing capacity is defined as number of target cells killed per effector cell, at an effector cell/target cell ratio of 1:1 during a 4-h in vitro assay.
Assuntos
Cromo/sangue , Testes Imunológicos de Citotoxicidade/métodos , Síndrome de Fadiga Crônica/imunologia , Células Matadoras Naturais/imunologia , Síndrome do Golfo Pérsico/imunologia , Psiconeuroimunologia/métodos , Bioensaio , Antígeno CD56/imunologia , Estudos de Casos e Controles , Cromo/imunologia , Síndrome de Fadiga Crônica/sangue , Síndrome de Fadiga Crônica/patologia , Citometria de Fluxo , Humanos , Células K562 , Células Matadoras Naturais/citologia , Síndrome do Golfo Pérsico/sangue , Síndrome do Golfo Pérsico/patologiaRESUMO
Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter will describe a chromium ((51)Cr) release bioassay designed to measure the target cell killing capacity of NK cells (NKCC). Key features of the cytotoxicity assay are that it is done with whole blood and that numbers of effector cells are determined for each sample by flow cytometry and lymphocyte count. Effector cells are defined as CD3-CD56+ lymphocytes. Target cells are the K562 eyrthroleukemia cell line. Killing capacity is defined as number of target cells killed per effector cell, at an effector cell/target cell ratio of 1:1 during a 4 h in vitro assay.