Validation of a New HPLC-DAD Method to Quantify 3-Deoxyanthocyanidins Based on Carajurin in Medicinal Plants and for Chemical Ecology Studies.

Anthocyanidins are found in nature mainly as pelargonidin, cyanidin, peonidin, delphinidin, petunidin, and malvidin derivatives. These compounds are found free or as glycoside derivatives which are responsible for the color (red, blue, and violet) of some foods and are responsible for attracting seed dispersers. They are grouped into 3-hydroxyanthocyanidins, 3-deoxyanthocyanidins (3D-anth), and O-methylated anthocyanidins. A new method was developed and validated to quantify 3D-anth in plant-rich extracts. To test the new method, Arrabidaea chica Verlot was selected as it is widely used in folk medicine, and it is rich in 3D-anth. The new method was developed by HPLC-DAD and expressed 3D-anth as carajurin content. Carajurin was chosen as the reference standard due to its role as a biological marker for the antileishmanial activity for A. chica. The selected method used a silica-based phenyl column, a mobile phase composed of potassium dihydrogen phosphate buffer, acetonitrile, and methanol, in a gradient elution mode and detection at 480 nm. The method reliability was confirmed by verifying selectivity, linearity, precision, recovery, and robustness. This method contributes to quality control and development of a possible active pharmaceutical ingredient from A. chica as well as it can be used to evaluate 3D-anth in plant extracts with chemical ecology interest.

Flavonols and Flavones as Potential anti-Inflammatory, Antioxidant, and Antibacterial Compounds.

Plant preparations have been used to treat various diseases and discussed for centuries. Research has advanced to discover and identify the plant components with beneficial effects and reveal their underlying mechanisms. Flavonoids are phytoconstituents with anti-inflammatory, antimutagenic, anticarcinogenic, and antimicrobial properties. Herein, we listed and contextualized various aspects of the protective effects of the flavonols quercetin, isoquercetin, kaempferol, and myricetin and the flavones luteolin, apigenin, 3',4'-dihydroxyflavone, baicalein, scutellarein, lucenin-2, vicenin-2, diosmetin, nobiletin, tangeretin, and 5-O-methyl-scutellarein. We presented their structural characteristics and subclasses, importance, occurrence, and food sources. The bioactive compounds present in our diet, such as fruits and vegetables, may affect the health and disease state. Therefore, we discussed the role of these compounds in inflammation, oxidative mechanisms, and bacterial metabolism; moreover, we discussed their synergism with antibiotics for better disease outcomes. Indiscriminate use of antibiotics allows the emergence of multidrug-resistant bacterial strains; thus, bioactive compounds may be used for adjuvant treatment of infectious diseases caused by resistant and opportunistic bacteria via direct and indirect mechanisms. We also focused on the reported mechanisms and intracellular targets of flavonols and flavones, which support their therapeutic role in inflammatory and infectious diseases.

Carajurin: a anthocyanidin from Arrabidaea chica as a potential biological marker of antileishmanial activity.

Leishmaniasis is a group of neglected tropical diseases whose treatment with antimonials bears limitations and has changed little in over 80 years. Medicinal plants have been evaluated as a therapeutic alternative for leishmaniasis. Arrabidaea chica is popularly used as a wound healing and antiparasitic agent, especially as leishmanicidal agent. This study examined the leishmanicidal activity of a crude extract (ACCE), an anthocyanidin-rich fraction (ACAF), and three isolated anthocyanidins from A. chica: carajurin, 3'-hydroxy-carajurone, and carajurone. We evaluated the antileishmanial activity against promastigote and intracellular amastigote forms of Leishmania amazonensis and determined cytotoxicity in BALB/c peritoneal macrophages, as well as nitrite quantification, using the Griess method. Molecular docking was carried out to evaluate interactions of carajurin at the nitric oxide synthase enzyme. All compounds were active against promastigotes after 72 h, with IC50 values of 101.5 ± 0.06 µg/mL for ACCE and 4.976 ± 1.09 µg/mL for ACAF. Anthocyanidins carajurin, 3'-hydroxy-carajurone, and carajurone had IC50 values of 3.66 ± 1.16, 22.70 ± 1.20, and 28.28 ± 0.07 µg/mL, respectively. The cytotoxicity assay after 72 h showed results ranging from 9.640 to 66.74 µg/mL for anthocyanidins. ACAF and carajurin showed selectivity against intracellular amastigote forms (SI> 10), with low cytotoxicity within 24 h, a statistically significant reduction in all infection parameters, and induced nitrite production. Molecular docking studies were developed to understand a possible mechanism of activation of the nitric oxide synthase enzyme, which leads to an increase in the production of nitric oxide observed in the other experiments reported. These results encourage us to suggest carajurin as a biological marker of A. chica.

Incentivo governamental para Arranjos Produtivos Locais de Plantas Medicinais e Fitoterápicos no âmbito do SUS / Government incentives for Local Productive Arrangements of Medicinal Plants and Herbal Medicines at Unified Health System (SUS)

O Ministério da Saúde vem publicando, desde 2012, editais para a seleção pública de projetos de Arranjos Produtivos Locais (APLs) em plantas medicinais e fitoterápicos no âmbito do SUS. APL pode ser definido como aglomeração de empresas, localizadas em um mesmo território, que apresentam especialização produtiva e mantêm algum vínculo de articulação, interação, cooperação e aprendizagem entre si e com outros atores locais, tais como: governo, associações empresariais, instituições de crédito, ensino e pesquisa. O presente estudo teve como objetivo descrever, de modo sistematizado e conciso, as informações essenciais sobre os APLs selecionados nos anos de 2012 e 2013. De dezembro de 2014 a março de 2015 foi solicitado aos coordenadores dos 23 APLs selecionados responder um questionário. Destes, 17 responderam. O recurso financeiro aportado pelo Ministério da Saúde possibilitou a aquisição de materiais de consumo e permanentes para a reestruturação dos setores de cultivo e de manipulação, bem como a realização de cursos de capacitação para profissionais de saúde e agricultores familiares envolvidos no projeto. Quanto aos problemas identificados, o mais citado pelos coordenadores foi a dificuldade na execução do recurso financeiro, o que resultou no atraso do início de alguns projetos. Contudo, o incentivo governamental para a formação de APLs consolida o serviço de fitoterapia na atenção primária à saúde no SUS.(AU)

The Ministry of Health has since 2012 been issuing public bids for the selection of local productive arrangements (LPAs) projects in the area of medicinal plants and herbal medicines, under the auspices of the government's Unified Health System (SUS). LPAs can be defined as groups or communities, located in the same area, which have a productive specialization and possess some type of cooperation and exchange of knowledge among themselves and with other local stakeholders such as the government, business associations, credit institutions, educational and research. The present study aimed to describe, in a systematic and concise way, the essential informations about the LPAs which were selected in the years 2012 and 2013. From December 2014 to March 2015 coordinators of 23 LPAs were requested to answer a questionnaire. Of these, 17 replied. The resources invested by the Ministry of Health enabled the LPAs to purchase supplies and equipment for cultivation and production, as well as to provide technical training courses for health professionals and agricultural workers involved in the project. The most problem reported was the management of financial resources, which resulted in startup delays of some projects. However, these government incentives for settling LPAs in Brazil has contributed to establishing phytotherapy in SUS clinics.(AU)