RESUMO
Cyclophosphamide (CP) is a commonly used anti-cancer drug which causes toxicity by its reactive metabolites such as acrolein and phosphoramide mustard. In the present study modulation of toxicity caused by concomitant exposure to CP and l-buthionine-SR-sulfoximine (BSO) by fenugreek (Trigonella foenum-graecum L.) extract was evaluated by measuring lipid peroxidation (LPO) and anti-oxidants in urinary bladder in mice. Fenugreek, a common dietary and medicinal herb, showed protective effect not only on LPO but also on the enzymatic anti-oxidants. CP-treated animals exhibited a significant decrease in the activities of glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GP) and catalase (CAT) when compared to the controls. Level of reduced glutathione (GSH) was also reduced with an increase in LPO in CP-treated animals. BSO treatment depicted an additive toxic effect in CP-treated animals. Pre-treatment of herbal extract restored activities of all the enzymes and thus showed an overall protective effect on additive effect of CP and BSO. Restoration of GSH by extract treatment may play an important role in reversing CP-induced apoptosis and free radical-mediated LPO in urinary bladder. Fenugreek, known for its hypoglycemic, anti-inflammatory and immunomodulatory activity, may be a promising protective medicinal herb for consideration in complementary therapy in cancer patients under chemotherapeutic interventions.
Assuntos
Butionina Sulfoximina/toxicidade , Ciclofosfamida/toxicidade , Extratos Vegetais/farmacologia , Trigonella/química , Doenças da Bexiga Urinária/induzido quimicamente , Doenças da Bexiga Urinária/prevenção & controle , Animais , Antioxidantes/metabolismo , Interações Medicamentosas , Glutationa/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Camundongos , Bexiga Urinária/efeitos dos fármacos , Bexiga Urinária/enzimologia , Bexiga Urinária/metabolismo , Doenças da Bexiga Urinária/enzimologia , Doenças da Bexiga Urinária/metabolismo , Água/químicaRESUMO
Manipulation of afferent inputs may temporarily modulate dystonic spasms. Ten patients with writer's cramp were enrolled in a double-blind, randomized, crossover study in which the effects of transcutaneous electrical stimulation (TENS) and placebo treatment were compared. Patients were evaluated using four measures of dystonic impairment. The TENS group showed a significant improvement that persisted for 3 weeks in three of the four measures.
Assuntos
Distúrbios Distônicos/terapia , Mãos/fisiopatologia , Recuperação de Função Fisiológica/fisiologia , Estimulação Elétrica Nervosa Transcutânea/métodos , Vias Aferentes/fisiologia , Avaliação da Deficiência , Distúrbios Distônicos/fisiopatologia , Mãos/inervação , Humanos , Neurônios Motores/fisiologia , Músculo Esquelético/inervação , Músculo Esquelético/fisiopatologia , Inibição Neural/fisiologia , Efeito Placebo , Células Receptoras Sensoriais/fisiologia , Medula Espinal/fisiologia , Transmissão Sináptica/fisiologia , Estimulação Elétrica Nervosa Transcutânea/estatística & dados numéricos , Resultado do TratamentoRESUMO
New data on diagnosis, drug therapy, surgery and psychosocial concerns have emerged since the publication of the 1998 Guidelines for the Management of Parkinson's Disease. This article reviews new data and addresses issues left unanswered in the previous guidelines.
Assuntos
Doença de Parkinson/terapia , Guias de Prática Clínica como Assunto , Idoso , Condução de Veículo/legislação & jurisprudência , Inibidores de Catecol O-Metiltransferase , Demência/etiologia , Demência/terapia , Depressão/diagnóstico , Depressão/etiologia , Depressão/terapia , Agonistas de Dopamina/uso terapêutico , Terapia por Estimulação Elétrica , Inibidores Enzimáticos/uso terapêutico , Globo Pálido/cirurgia , Humanos , Pessoa de Meia-Idade , Doença de Parkinson/diagnóstico , Doença de Parkinson/psicologia , Qualidade de Vida , Tálamo/cirurgia , Tomografia Computadorizada de Emissão de Fóton Único , Resultado do TratamentoRESUMO
Lactotransferrin is an iron-binding protein. It has been purified from buffalo colostrum. The purified lactotransferrin has been crystallized in 10% ethanol solution. The crystals are orthorhombic and the space group is P2(1)2(1)2(1) with unit cell dimensions a = 161.70 A, b = 155.75 A, c = 113.48 A. The asymmetric unit contains three molecules of the protein with a solvent content of about 59%. The crystals were stable in the X-ray beam and diffract beyond 3.5 A resolution. The native data have been collected and the structure determination is in progress.
Assuntos
Búfalos , Colostro/química , Lactoferrina/química , Animais , Fenômenos Químicos , Físico-Química , Cristalização , Feminino , Lactoferrina/isolamento & purificação , Difração de Raios XRESUMO
In a high proportion of Burkitt lymphomas, transcription of the c-myc gene is initiated from a cryptic promoter in the first intron, creating abnormal messenger RNA molecules in which intron sequences, normally spliced out of the nascent transcripts, persist. An antisense oligodeoxynucleotide directed against these intron sequences greatly inhibited the proliferation of Burkitt lymphoma cell lines containing the abnormal transcripts (ST486 and JD38), but not that of cell lines containing normal c-myc transcripts (KK124). Flow cytometry showed a pronounced reduction in intracellular c-myc protein levels in cell lines containing aberrant myc transcripts, but no change in other cellular proteins. Control oligonucleotide did not inhibit c-myc protein expression or growth. These experiments provide evidence that antisense oligonucleotides targeted against tumour-specific, aberrant RNA species could be effective in controlling the proliferation of tumour cells without affecting normal cells.
Assuntos
Linfoma de Burkitt/patologia , DNA de Neoplasias/efeitos dos fármacos , Íntrons/efeitos dos fármacos , Oligonucleotídeos/farmacologia , Oncogenes , Proteínas Proto-Oncogênicas/antagonistas & inibidores , Sequência de Bases , Linfoma de Burkitt/genética , Divisão Celular/efeitos dos fármacos , DNA de Neoplasias/genética , Avaliação Pré-Clínica de Medicamentos , Humanos , Dados de Sequência Molecular , Oligonucleotídeos/análise , Oligonucleotídeos Antissenso , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas c-myc , Fatores de TempoRESUMO
In twenty patients with chronic pain syndrome, acupuncture treatment resulted in significant improvement of both pain and psychiatric symptoms and higher plasma concentrations of metenkephalin. Plasma beta-endorphin concentrations were unchanged. The degree of symptom relief was correlated with the increase in plasma met-enkephalin.