RESUMO
A new rapid bioassay has been developed which can precisely differentiate between stereospecific alpha and beta isomers of the antimalarial drug arteether. This method was developed through the disc diffusion bioactivity tests wherein semisynthetically produced alpha arteether was able to inhibit the growth of E. coli strains which are defective in DNA gyrase enzyme. The wild type E. coli with intact DNA gyrase did not show this sensitivity to alpha arteether. The beta isomer of arteether was, however, ineffective against both the mutant and wild type strains. Direct experimental proof of gyrase involvement was obtained through mobilization of gyr genes by transformation of E. coli gyr- mutant strains with wild type gyrA clone pMK90 (carried on the thermo-inducible lambda Col E1 vector). This resulted in alpha arteether resistant and nalidixic acid sensitive phenotype clearly demonstrating the use of gyrA mutant strains in differentiating alpha and beta isomers of arteether by this simple bioassay.
Assuntos
Antimaláricos/farmacologia , Artemisininas , Escherichia coli/crescimento & desenvolvimento , Sesquiterpenos/farmacologia , Antimaláricos/química , Bioensaio , Cromatografia em Camada Fina , DNA Topoisomerases Tipo II/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Mutação , Plasmídeos , Sesquiterpenos/química , EstereoisomerismoRESUMO
We report in situ treatment, by focal hyperthermia, of a recurrent hepatoma in the transplanted liver of a 53-year-old man. Hyperthermia was generated by neodymium-yttrium-aluminum-garnet laser, which was delivered through a fiber placed inside a 19-gauge needle and inserted percutaneously into the liver under ultrasound guidance. The effect was monitored in real time by ultrasound and subsequently confirmed by computerized tomography and needle core biopsy. Objectively the tumor growth was halted for 3 months, indicating partial response to this minimally invasive treatment.
Assuntos
Carcinoma Hepatocelular/terapia , Hipertermia Induzida , Terapia a Laser , Neoplasias Hepáticas/terapia , Transplante de Fígado , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/patologia , Humanos , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Complicações Pós-Operatórias , Tomografia Computadorizada por Raios XRESUMO
The participants of inter-university "Kabaddi" competition showed higher values of height, weight and surface area than average Indian population, indicating better attainment of growth in them. Further, the values of respiratory efficiency tests like, FEV1, MEFR and PEFR were also observed to be more in these players, probably due to training effect. The grip strength values were high in comparison to those of Indian football goalkeepers and hockey players.