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1.
Plant Sci ; 277: 166-176, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30466582

RESUMO

BACKGROUND: The production of secondary metabolites through the culture of entire plants is of great interest. Soilless culture, such as hydroponics, enables the control of plant growth and metabolism. Specific environmental conditions must be developed to maximize the productivity of medicinal plants used as efficient natural bioreactors. METHODS: The nutrient solution of newly established hydroponic cultures ofDatura innoxia Mill. were inoculated with Agrobacterium rhizogenes (A.r.) wild strains (TR7, TR107, 11325 or 15834). Growth and the alkaloid contents of roots and aerial parts were analyzed. Axenic cultures were also performed with modified TR7 strains containing the egfp or gus reporter gene. In vitro isolated root cultures enabled the phenological and molecular demonstration of gene transfer. RESULTS: A.r.TR 7 led to a greater improvement in plant secondary metabolism and growth. Positive expression of the reporter genes occurred. Isolation and subculture of some of the roots of these plants showed a hairy root phenotype; molecular tests proved the transfer of bacterial genes into the roots isolated from the plants. CONCLUSIONS: Hyoscyamine and scopolamine productivity is enhanced after A.r. inoculation in the nutrient solution of hydroponic plants. Transformation events occur in the original roots of the plants. This leads to chimeric plants with a part of their roots harboring a hairy root phenotype. Such semi-composite plants could be used for successful specialized metabolite bioproduction in greenhouses.


Assuntos
Agrobacterium/patogenicidade , Alcaloides/metabolismo , Datura/metabolismo , Datura/microbiologia , Datura/crescimento & desenvolvimento , Hidroponia , Desenvolvimento Vegetal
2.
Arch Virol ; 161(8): 2273-8, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27180098

RESUMO

Although poleroviruses are known to infect a broad range of higher plants, carnivorous plants have not yet been reported as hosts. Here, we describe the first polerovirus naturally infecting the pitcher plant Nepenthes mirabilis. The virus was identified through bioinformatic analysis of NGS transcriptome data. The complete viral genome sequence was assembled from overlapping PCR fragments and shown to share 91.1 % nucleotide sequence identity with the US isolate of beet western yellows virus (BWYV). Further analysis of other N. mirabilis plants revealed the presence of additional BWYV isolates differing by several insertion/deletion mutations in ORF5.


Assuntos
Luteoviridae/isolamento & purificação , Magnoliopsida/virologia , Doenças das Plantas/virologia , Animais , Sequência de Bases , Beta vulgaris/virologia , Luteoviridae/classificação , Luteoviridae/genética , Luteoviridae/fisiologia , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Proteínas Virais/genética
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