Toxicological evaluation of rapeseed products in a subacute feeding study in rats.

Groups of 15 male rats were fed ad libitum for 4 weeks standard diet containing 0, 2.5, 5 or 10% rapeseed protein isolate (RPI), 2.5, 5 or 10% rapeseed extraction residue (RER) or 10% casein. Body weight gain and food intake were recorded weekly. Clinical chemistry analyses, haematology, urinalysis, organ weights and histology were performed terminally. Serum alanine aminotransferase was decreased by RPI, RER and casein, while serum albumin only increased in rats fed RER. Microscopic examinations revealed abnormalities in liver and kidneys of animals at the 10% RPI, RER and casein levels. The absolute liver weights showed increases in the 5 and 10% RER groups and in the 10% RPI group. The relative kidney weights were reduced at all RER levels and in the 2.5% RPI group. Antithyroid activity of the rapeseed products were not noted.

[Toxicological evaluation of saccharose carbonic acid esters in basic subchronic feeding trials with rats. 1. Effect of saccharose fatty acid polyester]. / Toxikologische Beurteilung von Saccharosecarbonsäureestern auf der Grundlage subchronischer Fütterungsversuche mit Ratten. 1. Mitt. Wirkung von Saccharosefettsäurepolyester.

Groups of male and female rats received a sucrose fatty acid polyesters containing product (SPE) in their diet for 13 weeks at levels of 0, 5, 10 or 15%. Additional groups were pair-fed to the high-dose SPE-group (standard diet, 92.5%) or given food containing 7.5% hydrogenated lard (HF) or 4.5% fatty acid ethyl ester (FEE). Compared to the controls, there were increases in the food intake in males and females of the SPE-groups, HF- group and FEE-group. Male rats fed SPE showed increases in serum urea nitrogen at all levels, in serum alkaline phosphatase activity and urinary glucose excretion at 10 and 15%, in serum leucine amino-peptidase at 15%. In females dietary SPE increased the blood glucose content and serum alkaline phosphatase activity at 15% and the serum leucine aminopeptidase activity at 10 and 15%.

[The subchronic toxicity of 5-vinyloxazolidine-2-thione in rats]. / Untersuchung der subchronischen Toxizität von 5-Vinyloxazolidin-2-thion an Ratten.

Groups of male and female rats received 5-vinyloxazolidine-2-thione (VOT) in their drinking water for 13 weeks at concentrations of 0, 10, 25, 50 or 100 mg/l and 0, 2.5 or 5 mg/l respectively. Compared with the controls, there were decreases in food intake and growth of females given VOT-doses of 10 mg/l and higher. Liquid intake was reduced in males and females of the highest dose group. Terminal haematological examination revealed an increase in white cell count at the 50 and 100 mg levels in females and a reduction in the red cell count at 100 mg/l in males. Organ weight changes were dose-related increases in the relative thyroid and liver weight and a decrease in the relative thymus weight. Structural and functional changes of the thyroid were observed in animals at levels of 10 mg/l and higher. Histological examinations of the kidneys showed tubulonephrosis in animals at and above a VOT-level of 5 mg/l. The no-observed-effect level (NOEL) established from these studies was 2.5 ml VOT/l drinking water (approximately equivalent to an daily intake of 0.4 mg/kg body weight).

[Progress report. Possibilities and limitations of the in vitro technic for prenatal toxicologic studies]. / Fortschrittsbericht. Grenzen und Möglichkeiten der In-vitro-Technik für pränataltoxikologische Untersuchungen.

Considering the variety of problems of the prenatal toxicity test the in vitro methods hitherto existing in this field are discussed according to their degree of complexity (cell culture, mass culture of dispersed cells, organ culture, whole embryo culture) within their limitations and possibilities. In this connection special attention is focussed to the use of human tissue for testing basic mechanisms of the developmental biology and for toxicity tests.

[Continuous interpretation system for the cytophotometer "Amplival photometry"=-studies with pesticide combinations]. / Kontinuierliches Auswertesystem zum Cytophotometer "Amplival photometrie"--Untersuchungen mit Pestizidkombinationen.

By means of complementary technique the cytophotometer "Amplival photonetrie" is converted in a continuous interpretation system. Thereby it is possible to move the slides by controlling step motors in alpha- and/or gamma-axis. The number of stepts and the speed of them can be predetermined. For the quantitative histochemical examination the sum of the extinctions of single steps is determined. therefore the arythmetical average can be calculated per arbitrary unit tissue. In examinations with combinations of pesticides the usefulness of the interpretation system is demonstrated.