RESUMO
Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1. The chemical and spectral methods revealed a main polymer chain consisting of (1 â 6) ß-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as ß-GlcpA-4-OMe-(1 â 6)-ß-Galp-(1â fragments. In addition, a small part of the main chain carries monosaccharide residues of ß-Galp-(1â in position C-2. The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.
Assuntos
Galactanos , Ferula , Peso Molecular , PrebióticosRESUMO
From the leaves of Silybum marianum L. were isolated arabinogalactan with molecular weight 38â¯kDa and pectic substances. The monosaccharide composition of arabinogalactan was represented by ß-galactose and α-arabinose in a ratio of 2.6:1.0 and ß-galacturonic acid as a minor component. By chemical methods and GC, GC-MS, 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d-galactopyranose residues linked by ß-1,6-glycosidic bonds as a main chain, and the side chain was represented by α-arabinose, ß-galactose and 4-O-methylglucuronic acid. Pectic substance was found in small amounts. According to NMR data it contains also a branched rhamnogalacturonan.