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Métodos Terapêuticos e Terapias MTCI
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1.
J Med Food ; 18(7): 745-52, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25599142

RESUMO

Daucus carota L. ssp. carota (Apiacea, wild carrot, Queen Anne's lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. This study aims to determine the anticancer activity of the pentane fraction (F1) and the 1:1 pentane:diethyl ether fraction (F2) of the Daucus Carota oil extract (DCOE) against human colon adenocarcinoma cell lines (HT-29 and Caco-2). Treatment of cells with various concentrations of F1 or F2 fractions produced a dose-dependent inhibition of cell proliferation. Flow cytometric analysis indicated that both fractions induced sub-G1 phase accumulation and increased apoptotic cell death. Western blot revealed the activation of caspase-3, PARP cleavage, and a considerable increase in Bax and p53 levels, and a decrease in Bcl-2 level. Treatment of HT-29 cells with either fraction markedly decreased the levels of both phosphorylated Erk and Akt. Furthermore, the combined treatment of F1 or F2 with wortmannin showed no added inhibition of cell survival suggesting an effect of F1 or F2 through the phosphatidyl inositol 3-kinase (PI3K) pathway. This study proposes that DCOE fractions (F1 and F2) inhibit cell proliferation by inducing cell cycle arrest and apoptosis in HT-29 cells through the suppression of mitogen-activated protein kinase (MAPK)/Erk and PI3K/Akt pathways.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Neoplasias do Colo/patologia , Daucus carota/química , Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores , Pentanos/farmacologia , Inibidores de Fosfoinositídeo-3 Quinase , Adenocarcinoma/patologia , Apoptose/efeitos dos fármacos , Células CACO-2 , Proliferação de Células/efeitos dos fármacos , Neoplasias do Colo/enzimologia , MAP Quinases Reguladas por Sinal Extracelular/análise , Fase G1/efeitos dos fármacos , Células HT29 , Humanos , Fitoterapia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Proteínas Proto-Oncogênicas c-akt/análise
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