RESUMO
The use of inorganic calcium/phosphate supplemented with biopolymers has drawn lots of attention in bone regenerative medicine. While inflammation is required for bone healing, its exacerbation alters tissue regeneration/implants integration. Inspired by bone composition, a friendly automated spray-assisted system was used to build bioactive and osteoinductive calcium phosphate/chitosan/hyaluronic acid substrate (CaP-CHI-HA). Exposing monocytes to CaP-CHI-HA resulted in a secretion of pro-healing VEGF and TGF-ß growth factors, TNF-α, MCP-1, IL-6 and IL-8 pro-inflammatory mediators but also IL-10 anti-inflammatory cytokine along with an inflammatory index below 1.5 (versus 2.5 and 7.5 following CaP and LPS stimulation, respectively). Although CD44 hyaluronic acid receptor seems not to be involved in the inflammatory regulation, results suggest a potential role of chemical composition and calcium release from build-up substrates, in affecting the intracellular expression of a calcium-sensing receptor. Herein, our findings indicate a great potential of CaP-CHI-HA in providing required inflammation-healing balance, favorable for bone healing/regeneration.
Assuntos
Substitutos Ósseos/farmacologia , Fosfatos de Cálcio/farmacologia , Quitosana/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Ácido Hialurônico/farmacologia , Regeneração Óssea/genética , Regeneração Óssea/imunologia , Substitutos Ósseos/química , Osso e Ossos/citologia , Osso e Ossos/metabolismo , Fosfatos de Cálcio/química , Quimiocina CCL2/genética , Quimiocina CCL2/imunologia , Quitosana/química , Regulação da Expressão Gênica/imunologia , Humanos , Receptores de Hialuronatos/genética , Receptores de Hialuronatos/imunologia , Ácido Hialurônico/química , Inflamação , Interleucinas/genética , Interleucinas/imunologia , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/imunologia , Mitocôndrias/metabolismo , Espécies Reativas de Oxigênio/imunologia , Espécies Reativas de Oxigênio/metabolismo , Receptores de Detecção de Cálcio/genética , Receptores de Detecção de Cálcio/imunologia , Transdução de Sinais , Células THP-1 , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/imunologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/imunologia , Vinculina/genética , Vinculina/imunologiaRESUMO
Thin films and surface coatings play an important role in basic and applied research. Here we report on a new, versatile, and simple method ("precipitation coating") for the preparation of inorganic films, based on the alternate spraying of complementary inorganic salt solutions against a receiving surface on which the inorganic deposit forms. The method applies whenever the solubility of the deposited material is smaller than that of the salts in the solutions of the reactants. The film thickness is controlled from nanometers to hundreds of micrometers simply by varying the number of spraying steps; 200 spray cycles, corresponding to less than 15 min deposition time, yield films with thicknesses exceeding one micrometer and reaching tens of micrometers in some cases. The new solution-based process is also compatible with conventional layer-by-layer assembly and permits the fabrication of multimaterial sandwich-like coatings.