RESUMO
In the present study, we describe the antinociceptive effect of filicene, a triterpene isolated from Adiantum cuneatum (Adiantaceae) leaves, in several models of pain in mice. When evaluated against acetic acid-induced abdominal constrictions, filicene (10, 30 and 60 mg/kg, i.p.) produced dose-related inhibition of the number of constrictions, being several times more potent [ID(50)=9.17 (6.27-13.18) mg/kg] than acetaminophen [ID(50)=18.8 (15.7-22.6) mg/kg], diclofenac [ID(50)=12.1(9.40-15.6) mg/kg] and acetylsalicylic acid [ID(50)=24.0(13.1-43.8) mg/kg] in the same doses as those used for the standard drugs. Filicene also produced dose-related inhibition of the pain caused by capsaicin and glutamate, with mean ID(50) values of 11.7 (8.51-16.0) mg/kg and <10 mg/kg, respectively. Its antinociceptive action was significantly reversed by atropine, haloperidol, GABA(A) and GABA(B) antagonists (bicuculline and phaclofen, respectively), but was not affected by L-arginine-nitric oxide, serotonin, adrenergic and the opioid systems. Together, these results indicate that the mechanisms involved in its action are not completely understood, but seem to involve interaction with the cholinergic, dopaminergic, glutamatergic, GABAergic and tachykinergic systems.
Assuntos
Adiantum/química , Analgésicos/isolamento & purificação , Analgésicos/farmacologia , Triterpenos/isolamento & purificação , Triterpenos/farmacologia , Ácido Acético/toxicidade , Analgésicos/administração & dosagem , Analgésicos/química , Animais , Capsaicina/toxicidade , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Ácido Glutâmico/toxicidade , Masculino , Camundongos , Estrutura Molecular , Dor/tratamento farmacológico , Dor/fisiopatologia , Fitoterapia , Plantas Medicinais/química , Receptores Colinérgicos/efeitos dos fármacos , Receptores Colinérgicos/fisiologia , Receptores Dopaminérgicos/efeitos dos fármacos , Receptores Dopaminérgicos/fisiologia , Receptores de GABA/efeitos dos fármacos , Receptores de GABA/fisiologia , Receptores de Neurotransmissores/efeitos dos fármacos , Receptores de Neurotransmissores/fisiologia , Receptores de Taquicininas/efeitos dos fármacos , Receptores de Taquicininas/fisiologia , Triterpenos/administração & dosagem , Triterpenos/químicaRESUMO
Wedelia paludosa (Acmela brasiliensis) (Asteraceae), a traditionally used native Brazilian medicinal plant, showed antifungal activity against dermatophytes in dilution tests. The hexane, dichloromethane and butanol fractions displayed activity against Epidermophyton floccosum, Trichophyton rubrum and Trichophyton mentagrophytes, with minimal inhibitory concentrations between 250 and 1000 microg/mL. Two pure compounds, identified as kaurenoic acid (1) and luteolin (2), also showed activity against these dermatophytes.
Assuntos
Antifúngicos/farmacologia , Fungos/efeitos dos fármacos , Wedelia/química , Antifúngicos/isolamento & purificação , Meios de Cultura , Diterpenos/farmacologia , Flavonoides/farmacologia , Flores/química , Luteolina , Metanol , Testes de Sensibilidade Microbiana , Extratos Vegetais/química , Extratos Vegetais/farmacologiaRESUMO
This study was conducted in order to determine the chemical composition and the possible antinociceptive effects in mice of some species of Phyllanthus in vitro. The methanolic extracts obtained from callus cultures of P. fraternus, P. stipulatus and P. caroliniensis caused significant inhibition in to the late phase of the formalin test, whereas the extract from P. urinaria inhibited both neurogenic and inflammatory phases of the test. Conventional chromatographic methods (TLC, GC) permitted the detection of some steroids or triterpenes, including beta-sitosterol, glochidonol and glochidone, which seem be responsible for the antinociceptive effects of the callus extracts studied.