RESUMO
Trace metals are chemical pollutants that have well-known noxious effects on wildlife and that are current major environmental issues in urban habitats. Previous studies have demonstrated their negative (e.g. lead) or positive (e.g. zinc) effects on body condition, immunity and reproductive success. Because of their effects on condition, trace metals are likely to influence the production of condition-dependent ornaments. The last decade has revealed that bird odors, like mammal odors, can convey information on individual quality and might be used as secondary sexual ornaments. Here, we used solid-phase microextraction headspace sampling with gas chromatography-mass spectrometry to investigate whether plumage scent varied with experimental supplementation in lead and/or zinc in feral pigeons. Zinc supplementation (alone or in combination with lead) changed the proportion of several volatiles, including an increase in the proportion of hydroxy-esters. The production of these esters, that most likely originate from preen gland secretions, may be costly and might thus be reduced by stress induced by zinc deficiency. Although lead is known to negatively impact pigeon condition, it did not statistically affect feather scent, despite most of the volatiles that increased with zinc exposure tended to be decreased in lead-supplemented pigeons. Further studies should evaluate the functions of plumage volatiles to predict how trace metals can impact bird fitness.
Assuntos
Columbidae/metabolismo , Poluentes Ambientais/metabolismo , Chumbo/metabolismo , Odorantes/análise , Zinco/metabolismo , Animais , Plumas/química , Feminino , Masculino , Oligoelementos/metabolismoRESUMO
Twelve French Asplenioideae ferns (genera Asplenium and subgenera Ceterach and Phyllitis) were investigated for the first time for volatile organic compounds (VOC) using GC-MS. Sixty-two VOC biosynthesized from the lipidic, shikimic, terpenic and carotenoid pathways were identified. Several VOC profiles can be highlighted from Asplenium jahandiezii and A. xalternifolium with exclusively lipidic derivatives to A. onopteris with an equal ratio of lipidic/shikimic compounds. Very few terpenes as caryophyllene derivatives were identified, but only in A. obovatum subsp. bilotii. The main odorous lipidic derivatives were (E)-2-decenal (waxy and fatty odor), nonanal (aldehydic and waxy odor with a fresh green nuance), (E)-2-heptenal (green odor with a fatty note) and 1-octen-3-ol (mushroom-like odor), reported for all species. A few VOC are present in several species in high content, i.e., 9-oxononanoic acid used as a precursor for biopolymers (19% in A. jahandiezii), 4-hydroxyacetophenone with a sweet and heavy floral odor (17.1% in A. onopteris), and 4-hydroxybenzoic acid used as a precursor in the synthesis of parabens (11.3% in A. foreziense). Most of the identified compounds have pharmacological activities, i.e., octanoic acid as antimicrobial, in particular against Salmonellas, with fatty and waxy odor (41.1% in A. petrarchae), tetradecanoic acid with trypanocidal activity (13.3% in A. obovatum subsp. bilotii), 4-hydroxybenzoic acid (8.7% in A. onopteris) with antimicrobial and anti-aging effects, 3,4-dihydroxybenzaldehyde as an inhibitor of growth of human cancer cells (6.7% in Ceterach officinarum), and phenylacetic acid with antifungal and antibacterial activities (5.8% in A. onopteris). Propionylfilicinic acid was identified in the twelve species. The broad spectrum of odorous and bioactive VOC identified from the Asplenium, Ceterach and Phyllitis species are indeed of great interest to the cosmetic and food industries.
Assuntos
Gleiquênias/química , Extratos Vegetais/química , Compostos Orgânicos Voláteis/química , Aldeídos/química , Aldeídos/isolamento & purificação , Aldeídos/farmacologia , Benzaldeídos/química , Benzaldeídos/isolamento & purificação , Benzaldeídos/farmacologia , Catecóis/química , Catecóis/isolamento & purificação , Catecóis/farmacologia , Gleiquênias/classificação , Octanóis/química , Octanóis/isolamento & purificação , Octanóis/farmacologia , Parabenos/química , Parabenos/isolamento & purificação , Parabenos/farmacologia , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Compostos Orgânicos Voláteis/isolamento & purificação , Compostos Orgânicos Voláteis/farmacologiaRESUMO
Aerial parts of six Dryopteris species collected in France were investigated for volatile organic compounds (VOC) for the first time. Fifty-three biosynthesized VOC from the shikimic, lipidic and terpenic pathways were identified using gas chromatography/mass spectrometry. Many bioactive polyketide compounds as filicinic derivatives (from 8.5 to 23.5%) and phloroglucinol derivatives (from 8.2 to 53.8%) with various pharmacological activities were detected in high amount from five analysed Dryopteris species, in particular D. oreades and D. borreri, i.e., propionylfilicinic acid (> 10% in D. affinis and D. ardechensis) and 2,6-dihydroxy-4-methoxy-3-methylbutyrophenone (aspidinol) (19.1% and 14.6% in D. oreades and D. borreri, respectively). Several terpenic derivatives with a low odor threshold were identified, i.e., carota-5,8-diene (from 2.5 to 18.4%: floral, woody or fresh bark note), (E)-nerolidol (> 10% for D. borreri and D. cambrensis; floral or woody odor), alpha-selinene (> 7% for D. ardechensis; woody-spicy odor), and aristolene (12.8% in D. affinis; flower, sweet odor). The main isoprenoid derivatives were 4-hydroxy-5,6-epoxyionol, 3-oxo-alpha-ionol and 4-oxo-7,8-dihydro-beta-ionone (essentially in D. remota), whereas the main aromatic compound was 4-hydroxy-3-methoxyacetophenone (20.6% and 12.6% in D. cambrensis and D. borreri, respectively) and the main lipid derivative was 1-octen-3-ol with a mushroom-like odor (from 0.4 to 8.3%). Dryopteris species resources are of great interest as a reservoir of odorous and bioactive compounds.
Assuntos
Dryopteris/química , Compostos Orgânicos Voláteis/análise , França , OdorantesRESUMO
Six horsetails were investigated for volatile organic compounds (VOC) by GC-MS using organic solvent extraction. Seventy-five VOC biosynthesized from the shikimic, lipidic and terpenic pathways including isoprenoid derivatives were detected from these putative natural resources. E. palustre var. americana contained mainly lipidic derivatives, i.e., 1-octen-3-ol (mushroom-like odor), (E)-2-hexenoic acid (fruity odor) and (E)-2-hexenal (green odor). Many isoprenoid flavour precursors, i.e., 3-oxo-alpha-ionol (spicy odor) and (E,E)-pseudoionone (balsamic odor), as well as odorous benzenic derivatives, i.e, phenylethanal (hyacinth, lilac note) and 2-phenylethanol (rose odor) contributed to the odor of E. arvense. The volatile pattern of E. telmateia is dominated by high amounts of isoprenoids and benzenic derivatives. The complex volatile profiles of E. hyemale and E. ramosissimum are based on ferulic acid isomers, along with either (E)-2-heptenal (green vegetable-like odor) or 4-vinylguaiacol (spicy clove smoky odor) for E. hyemale and E. ramosissimum, respectively. The broad spectrum of E. scirpioides shows the lowest VOC content with high amount of isoprenoids (46.9%), mainly ionone derivatives. Equisetum resources are of great interest as bioactive litter and new potential functional feed ingredients.
Assuntos
Equisetum/química , Compostos Orgânicos Voláteis/análise , Cromatografia Gasosa-Espectrometria de Massas , VolatilizaçãoRESUMO
Stoichiometric homeostasis of heterotrophs is a common, but not always well-examined premise in ecological stoichiometry. We experimentally evaluated the relationship between substrate (plant litter) and consumer (microorganisms) stoichiometry for a tropical terrestrial decomposer system. Variation in microbial C : P and N : P ratios tracked that of the soluble litter fraction, but not that of bulk leaf litter material. Microbial N and P were not isometrically related, suggesting higher rates of P than N sequestration in microbial biomass. Shifts in microbial stoichiometry were related to changes in microbial community structure. Our results indicate that P in dissolved form is a major driver of terrestrial microbial stoichiometry, similar to aquatic environments. The demonstrated relative plasticity in microbial C : P and N : P and the critical role of P have important implications for theoretical modelling and contribute to a process-based understanding of stoichiometric relationships and the flow of elements across trophic levels in decomposer systems.
Assuntos
Consórcios Microbianos/fisiologia , Folhas de Planta/microbiologia , Biomassa , Carbono/metabolismo , Ecossistema , Meliaceae/microbiologia , Nitrogênio/metabolismo , Fósforo/metabolismo , Clima TropicalRESUMO
Six French ferns were investigated for volatile organic compounds (VOC) by GC-MS using organic solvent extraction. Seventy-seven VOC biosynthesized from the shikimic, lipidic and terpenic pathways, including isoprenoid derivatives, were identified from these putative natural resources. Asplenium trichomanes subsp. trichomanes contained mainly polyketides with an oily or waxy odor. (E)-2-Hexenal and (Z)-3-hexenol, responsible for the "green odor", were found in high contents in Polystichum setiferum, Dryopteris dilatata and Phegopteris connectilis. In the last, 7.4% of coumarin with a cut hay scent was highlighted from the volatile fraction. (E)-3-Hexenoic acid and (E)-2-hexenoic acid, both with herbal and fruity notes, were identified in Gymnocarpium dryopteris and Pteridium aquilinum. 1-Octen-3-ol, well-known for its mushroom-like odor, was abundant in all analyzed French ferns. While the "fougère" fragrance is claimed by the perfumers to be a fantasy scent, coumarin, (E)-2-hexenal, (Z)-3-hexenol and 1-octen-3-ol are the main odorous components of the perfumes belonging to the fougère accord family. This suggests that the fougère scent from the perfumers' imagination is a natural fragrance.
Assuntos
Gleiquênias/química , Odorantes , Compostos Orgânicos Voláteis/análise , FrançaRESUMO
Five French ferns belonging to different families were investigated for volatile organic compounds (VOC) by GC-MS using organic solvent extraction. Fifty-five VOC biosynthesized from the shikimic, lipidic and terpenic pathways including monoterpenes, sesquiterpenes and carotenoid-type compounds were identified. The main volatile compound of Adiantum capillus-veneris L. (Pteridaceae) was (E)-2-decenal with a plastic or "stink bug" odor. The volatile profiles of Athyrium filix-femina (L.) Roth (Woodsiaceae) and Blechnum spicant (L.) Roth (Blechnaceae) showed similarities, with small amounts of isoprenoids and the same main volatile compounds, i.e., 2-phenylethanal (odor of lilac and hyacinth) and 1-octen-3-ol (mushroom-like odor). The main volatile compound of Dryopteris filix-mas (L.) Schott (Dryopteridaceae) was (E)-nerolidol with a woody or fresh bark note. Polyketides, as acylfilicinic acids, were mainly identified in this fern. Oreopteris limbosperma (Bellardi ex. All.) J. Holub (Thelypteridaceae), well-known for its lemon smell, contained the highest biodiversity of VOC. Eighty percent of the volatiles was issued from the terpenic pathway. The main volatiles were (E)-nerolidol, alpha-terpineol, beta-caryophyllene and other minor monoterpenes (for example, linalool, pinenes, limonene, and gamma-terpinen-7-al). It was also the fern with the highest number of carotenoid-type derivatives, which were identified in large amounts. Our results were of great interest underlying new industrial valorisation for ferns based on their broad spectrum of volatiles.