RESUMO
To study the substitution of fish oil by vegetable oils in fish diets, juveniles Senegalese sole (Solea senegalensis) were fed diets (56 % crude protein, 12 % crude lipid) containing either linseed (100LO) or soybean (100SO) oils in comparison with a 100 % fish oil-based diet (100FO) for 90 days. Samples of muscle, liver, and intestine were collected for biochemical analysis and for glucocorticoid receptor-related genes, including GR1 and GR2, and the associated heat shock proteins HSP70, HSP90AA, and HSP90AB. Besides, basal levels of plasma cortisol were also determined. After the feeding period, a stress test, consisting on 5 min of net chasing, was applied to a selected population of each dietary group. Total replacement of fish oil by vegetable oils did not induced changes in fish growth and performance, but affected fatty acid profile of muscle, liver, and intestine, reflecting those tissues the characteristic fatty acids of each type of dietary oil. A tendency to conserve the ARA/EPA ratio could be observed in the different tissues, despite of the level of these fatty acids in diet. Chasing stress induced an increase of muscle GR1 and a reduction in intestinal GR2 relative expressions at any of the experimental diets assayed. In liver, chasing stress induced an increase in both GR1 and GR2 gene expression in fish fed fish oil diets. Similarly, chasing stress induced an increase of muscle HSP70 and decrease of HSP90AB in liver at any of the experimental diet assayed. Besides, vegetable oils decreased the expression of HSP70 in intestine, being the relative expression of liver HSP90AA increased by the inclusion of linseed oil in the diet, at any of the experimental conditions assayed.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal/fisiologia , Aquicultura/métodos , Gorduras Insaturadas na Dieta/farmacologia , Linguados/fisiologia , Regulação da Expressão Gênica/efeitos dos fármacos , Óleos de Plantas/farmacologia , Análise de Variância , Animais , Sequência de Bases , Primers do DNA/genética , Gorduras Insaturadas na Dieta/administração & dosagem , Ácidos Graxos/análise , Óleos de Peixe/administração & dosagem , Proteínas de Choque Térmico/metabolismo , Hidrocortisona/sangue , Mucosa Intestinal/metabolismo , Fígado/metabolismo , Dados de Sequência Molecular , Músculo Esquelético/metabolismo , Óleos de Plantas/administração & dosagem , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Receptores de Glucocorticoides/genética , Receptores de Glucocorticoides/metabolismo , Análise de Sequência de DNA/veterinária , Estresse Fisiológico/fisiologiaRESUMO
A rare case of arthritis, peri-arthiritis and pleurits associated with Salmonella enterica and Corynebacterium pseudotuberculosis infection in a dromedary camel is reported. Articular infections caused by Non-typhoidal Salmonella have been exceptionally described in human medicine. To our knowledge, this would be the first description of articular infections associated with Non-thyphoidal Salmonella in other mammals than humans. Possible pathogenesis of the infection is discussed.