1.
J Microbiol Methods
; 150: 1-4, 2018 07.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29763647
RESUMO
RNA polymerase containing the stress response regulator σS subunit (RpoS) plays a key role in bacterial survival in hostile environments in nature and during infection. Here we devise and validate a simple cell-based high throughput luminescence assay for this holoenzyme suitable for screening large chemical libraries in a robotic platform.