Supplementation of nano-zinc in lower doses as an alternative to pharmacological doses of ZnO in weanling pigs.

A set of studies was performed to determine the influence of dietary ZnO concentration and source during two phases (days 0 to 14 and days 15 to 28). Experiment 1: 168 weaned piglets were allocated to four treatment groups in six replicates. The treatments included a basal diet without ZnO supplementation (control), 2,500 mg ZnO/kg (In2500), 500 mg nano-ZnO/kg (N500), and 150 mg nano-ZnO/kg (N150). Experiment 2: 168 weaned piglets were divided into three treatment groups with eight replicates. The treatments included control, In2500, N300, and 150 mg nano-ZnO/kg (N150). An in vitro trial showed that the growth of Listeria monocytogenes, Escherichia coli, and Salmonella typhimurium was inhibited when exposed to 300 and 500 ppm of ZnO after 24 h of incubation. In experiment 1, the average daily gain (ADG) by the pigs was improved in the N500 and IN2500 treatment groups. Colonization of coliforms and Clostridium spp. significantly decreased in the pigs fed the N500 and IN2500 diets in phase 1. The total plasma antioxidant capacity was greater in the IN2500 and N500 treatment groups than in the control. Superoxide dismutase (SOD) activity was greater in pigs fed the IN2500 (phase 1) or the IN2500 and N500 (phase 2) diets than in the control and N150 treatment group. In experiment 2, pigs in the N300 treatment group showed a higher ADG and lower fecal score colonization of coliforms and Clostridium spp. compared with those in the N150 treatment group. In conclusion, nano-ZnO at a dose of 300 ppm showed the same growth as the pharmacological dose of Zn. This provides an option to the pharmacological dose.

Improved Growth Performance, Antioxidant Status, Digestive Enzymes, Nutrient Digestibility and Zinc Bioavailability of Broiler Chickens with Nano-Sized Hot-Melt Extruded Zinc Sulfate.

This experiment was conducted to investigate the effect of the supplementation of hot-melt extrusion (HME) processed zinc sulfate (ZnSO4) on the growth performance, antioxidative activity, pancreatic digestive enzyme, small intestinal morphology, nutrient digestibility, and Zn content in broilers. The chicks were allocated to three treatments, each of which had five replicates of 15 chicks per replicate. The broiler chickens were assigned to three dietary treatments: the control (without supplemental Zn), IN-Zn (ZnSO4, 80 mg/kg), and HME-Zn (HME processed ZnSO4 as nano-Zn, 80 mg/kg). The broilers fed diets supplemented with 80 mg/kg of HME-Zn improved the BWG (P < 0.05) and FCR (P < 0.05) compared to the broilers fed the control and IN-Zn diets in phase 2. The Zn supplementation significantly enhanced the superoxide dismutase (SOD) activity in the serum (P < 0.05) and liver (P < 0.05), and HME-Zn supplementation significantly increased the SOD in the liver compared to the IN-Zn supplementation. Reduced malondialdehyde (MDA) concentration was seen with the Zn supplementation compared to the control (P < 0.05). The chickens fed diets supplemented with the HME-Zn had higher activity of amylase (P < 0.05) and trypsin (P < 0.05) than those of the chickens fed the control and IN-Zn diets. The villus height (VH) in the duodenum (P < 0.05) and jejunum (P < 0.05) increased with the ZnSO4 and HME-Zn supplementation compared to the control. The VH and crypt depth rate (VH:CD) in the jejunum improved with the HME-Zn compared to the control (P < 0.05). The HME-Zn significantly increased the apparent ileal digestible crude protein (CP) (P < 0.05) and energy corrected by nitrogen (AIDEn) (P < 0.05) compared to the control or IN-Zn. In phases 1 and 2, the HME-Zn significantly increased Zn concentration in the liver and tibia compared to control and IN-Zn (P < 0.05). The excretion of Zn was significantly decreased in the HME-Zn compared to the IN-Zn (P < 0.05). In conclusion, supplementation of 80 mg/kg of HME-Zn in diets improved the growth performance, antioxidative activity, pancreatic enzyme activity, intestinal villus height, and nutrient digestibility with the improved Zn bioavailability in broilers.

Effects of hot-melt extruded nano-copper on the Cu bioavailability and growth of broiler chickens.

This study was aimed to investigate the Cu bioavailability, growth response, digestibility of nutrients, and blood metabolites of broiler chicks fed CuSO4 in nano or common forms. A total of 720 broiler chickens were distributed between eight treatments according to a completely randomized design. There were 8 treatments and 6 replicates in each treatment with 15 birds/replicate. The treatments were divided into common copper sulfate at the doses of 16 ppm, 40 ppm, 80 ppm, and 120 ppm (INO) and hot-melt extruded copper sulfate at the doses of 16 ppm, 40 ppm, 80 ppm, and 120 ppm (HME-Cu). The experiment was operated for 35 days in 2 phases (phase 1, d 0 to 14; and phase 2, d 15 to 35). No significant differences were shown in growth performance, feed intake, FCR, and nutrient digestibility among the treatments. The concentration of Cu in the serum was increased in the HME-Cu broilers compared with the INO broilers at phase 2. A linear increase was observed in the concentration of Cu in the liver in broilers fed INO diets, however, no significant differences were observed by the supplementation of HME-Cu levels. The linear increase was detected in the content of Cu in excreta in the INO and HME-Cu treatments by increasing the dietary Cu content. The HME-Cu treatments showed a lower Cu concentration in the excreta compared with the INO treatments. The higher bioavailability of Cu in HME form can decrease the recommended dose of Cu in broiler diets.

Nano-sized Zinc in Broiler Chickens: Effects on Growth Performance, Zinc Concentration in Organs, and Intestinal Morphology.

The effects of dietary supplementation of zinc (Zn) sources and concentrations were investigated on growth performance, absorption into tissues, fecal excretion, nutrient retention, and intestinal morphology in broilers fed a corn-soybean meal basal diet. A total of 525 one-day-old chicks (Ross 308) were assigned based on body weight to seven dietary treatments. There were five replicate pens for each treatment and 15 broilers per replicate pen. The dietary treatments included a basal diet (control, without supplementing Zn), and basal diet supplemented with Zn, as inorganic zinc sulfate (ZnS; 110 mg/kg); organic Zn-methionine (ZnM; 110 mg/kg); hot-melt extruded (HME) 25 zinc sulfate (27.5 mg/kg); HME50 zinc sulfate (55 mg/kg); HME75 zinc sulfate (82.5 mg/kg); or HME100 zinc sulfate (110 mg/kg) for 35 days in two phases (d 1-21, phase I and d 22-35, phase II). Bodyweight and feed efficiency of broiler chicks fed diets supplemented with increasing dietary concentrations of HME-Zn improved linearly during the study period (P<0.05). Compared to the control treatment, the ZnS, ZnM, and HME diets increased Zn concentrations in the serum and liver. Inorganic ZnS supply resulted in the highest Zn concentration in excreta. Increasing supplemented Zn content in diets as HME linearly increased Zn concentration in the excreta, serum, liver, and tibia. Broiler chicks fed diets supplemented with increasing concentrations of HME increased villus height (VH; linear and quadratic) of the jejunum and VH of the ileum (linear). Increasing concentrations of dietary Zn supplied as HME resulted in linearly enhanced dry matter, gross energy, and nitrogen retention of broilers on day 21. These results suggest that dietary HME-Zn at a lower level (55 ppm) shows the same growth performance as common ZnSO4 at 110 ppm.

Synergistic effect of exogenous multi-enzyme and phytase on growth performance, nutrients digestibility, blood metabolites, intestinal microflora and morphology in broilers fed corn-wheat-soybean meal diets.

OBJECTIVE: This study was conducted to investigate the synergistic effect of exogenous multienzyme and phytase on growth performance, nutrients digestibility, blood metabolites, intestinal microflora, and morphology in broilers fed corn-wheat-soybean meal diets. METHODS: A 2×2 factorial design was used in this study. Four dietary treatments consisted of i) basal diets (corn-wheat-soybean meal based diets without multi-enzyme and phytase), ii) basal diets with phytase (0.05%), iii) basal diets with exogenous multi-enzyme (0.05%), and iv) basal diets with exogenous multi-enzyme including phytase (0.05%). A total of 480 broiler chickens (Ross 308 - one day old) were weighed and allotted to thirty-two cages (15 birds per cage), and chicks were randomly allocated to four dietary treatments. RESULTS: The body weight gain and feed conversion rate were improved by supplementation of exogenous multi-enzyme containing phytase during the finisher period (p<0.05). The birds fed diets with exogenous multi-enzyme containing phytase had a significantly greater digestibility of dry matter, gross energy, crude protein, calcium, and phosphorus compared with birds fed non-supplemented diets (p<0.05). The chickens fed diets with exogenous multi-enzyme containing phytase showed a higher concentration of Ca and P in the serum (p<0.05). The population of Lactobacillus spp., Escherichia coli, and Clostridium were not affected in the ileum and cecum of chickens fed enzyme-supplemented diets. The dietary supplemental exogenous multi-enzyme containing phytase showed a significant improvement in villus height, crypt depth, and villus height and crypt depth ratio, compared to basal diets or dietary supplemental phytase (p<0.05). CONCLUSION: The supplementation of the exogenous multi-enzyme containing phytase synergistically improved the growth performance, nutrients digestibility, and villus height of the small intestine of broiler chickens fed a corn-wheat-soybean meal based diets.

Effects of Hot-Melt Extruded Nano-Copper as an Alternative for the Pharmacological Dose of Copper Sulfate in Weanling Pigs.

This study was conducted to investigate the effects of hot-melt extrusion (HME)-processed copper (Cu) sulfate supplementation on the growth performance, gut microbiota, metabolic function of Cu, and bioavailability of Cu in weanling pigs fed a corn-soybean meal basal diets. A total of 180 piglets (Yorkshire × Landrace × Duroc) of mixed-sex randomly were allotted to six treatments on the basis of initial average body weight (6.36 ± 0.39 kg) to six dietary treatments. There were six replicates in each treatment with 5 pigs per replicates. The dietary treatments included levels of CuSO4 (IN6, 6 mg Cu/kg diets; IN125, 125 mg Cu/kg diets), nano-CuSO4 (HME6, 6 mg Cu/kg diets; HME65, 65 mg Cu/kg diets; and HME125, 125 mg Cu/kg diets), and Cu-methionine (ORG125, 125 mg Cu/kg diets). The weanling pigs fed diets supplemented with the HME65 and HME125 showed a greater body weight and feed intake compared with IN6 and IN125 (P < 0.05). The weaning pigs fed diets supplemented with the HME125 showed the highest digestibility of gross energy in phase 1 and phase 2 (P < 0.05). The supplementation of HME125 significantly reduced the Escherichia coli (E.coli) in cecum and colon (P < 0.05). The supplementation of HME65 showed statistically equivalent effect on reduction of E. coli in the cecum and colon compared with IN125 and ORG125 treatments. The villus height in duodenum and jejunum of piglets in HME65 and HME125 treatments were higher than ORG125, HME6, IN6, and IN125 (P < 0.05). The gene expression of Atox1 was upregulated in IN125, HME125, and ORG125 treatments (P < 0.05). The expression of Sod1 was increased in IN125 treatment compared with IN6 treatment (P < 0.05). The HME125 treatment had the highest gene expression of ghrelin (P < 0.05). The Cu concentration of serum and liver was higher in the HME125 treatment than the HME6, IN6, and IN125 treatments (P < 0.05). The HME125 and ORG125 treatments showed a lower fecal Cu compared with IN125 treatment (P < 0.05). Taken together, these results suggest that the HME65 can be an alternative to IN125 in weanling pigs due to the greater overall average daily gain, improved villus height, and higher bioavailability.

Hot-Melt Extruded Selenium: a Highly Absorbable Nano-Selenium in Lactating Sows Exposed to High Ambient Temperature.

To study the role of hot-melt extruded (HME) selenium (Se) and in the lactating sows' reproductive performance and litter growth, a total of 60 crossbred sows (Yorkshire × Landrace; average body weight, 245.3 kg) were allotted to one of four treatments according to a 2 × 2 factorial arrangement. The performance of lactating sows was investigated in the different average room temperatures (high temperature (HT), 28.1 °C vs. low temperature (LT), 24.6 °C), and different selenium sources (INO, 0.15 mg common sodium selenite/kg diet; HME, 0.15 mg nano-sodium selenite/kg diet) during the summer season. At days 10 and 21, the serum glutathione peroxidase (GPx) activity and Se concentration were greater in lactating sows fed HME compared with sows fed INO diet. The concentration of Se in the serum was greater in piglets from the HME treatment compared with piglets from the INO treatment. The HT treatment had a significantly higher body weight loss, backfat thickness loss, and weaning-to-estrus interval. The piglets' survivability, total weight gain, daily feed intake, and average daily gain during the lactation were greater in the LT sows compared with the HT sows. A higher blood cortisol concentration was observed in the HT sows compared with the LT. Selenium contents of milk at days 10 and 21 postpartum were increased by dietary supplementation of HME. In conclusion, the results of the present study demonstrated that there are no performance differences between sows fed different Se sources; however, HME supplementation increased serum and milk Se content, resulting in increased serum GPx concentration in sows and a greater serum Se in the litters.

Biological Evaluation of Hot-Melt Extruded Nano-selenium and the Role of Selenium on the Expression Profiles of Selenium-Dependent Antioxidant Enzymes in Chickens.

A study was conducted to determine the effect of dietary selenium (Se) concentration and source for broiler chickens on performance, nutrient digestibility, plasma Se, glutathione peroxidase (GPx) activity, and thiobarbituric acid reactive substances (TBARS). A total of 700 1-day-old broiler chicks were assigned to 7 diets with 20 birds per cage and 5 replicates per treatment. The experimental diets were fed for 32 days in 2 phases (phase 1, day 0 to 14 and phase 2, day 15 to 32). Treatments were as follows: control (without Se supplementation), sodium selenite (SeS; 0.15, 0.30, or 0.45 ppm), and hot-melt extruded sodium selenite (SeHME; 0.15, 0.30, or 0.45 ppm). There were significant linear responses (P < 0.01) for higher plasma Se concentration in SeS and SeHME treatments. Moreover, an increased (P < 0.01) Se concentration of plasma occurred in SeHME treatment compared with that in SeS treatment. The serum GPx analyses revealed that supplemental SeS and SeHME increased significantly the activity of GPx in the plasma in phase 1 (P < 0.05) and phase 2 (P < 0.05). There were significant linear (P < 0.01) responses of SeS and SeHME treatments for the expression of SelW, GPx1, GPx3, and GPx4 in the livers and spleens. In addition, SeHME showed an upregulated expression of GPx-4 in the livers (P < 0.01) and SelW in the spleens (P < 0.05) compared with SeS treatment. SeHME showed a lower TBARS on day 9. Moreover, a decreased (P < 0.01) TBARS occurred in SeS treatment compared with that in control treatment. In conclusion, SeHME can increase antioxidant activity and Se absorption, consequently being a more suitable source of Se than regular sodium selenite.

Preparation of cupric sulfate-based self-emulsifiable nanocomposites and their application to the photothermal therapy of colon adenocarcinoma.

Nanocomposites (NCs) of cupric sulfate monohydrate (CuSO4) were fabricated by hot-melt extrusion (HME) system equipped with twin screws. Micron-sized bulk powder of CuSO4 was dispersed in the mixture of surfactants (Span 80 and Tween 80) and hydrophilic polymer (polyethylene glycol (PEG) 6000) by HME process. Reduction of surface tension by surfactants and homogeneous dispersion in hydrophilic polymer along with HME technique were introduced to prepare CuSO4 NCs. Dispersion of CuSO4 NCs exhibited approximately 204 nm hydrodynamic size, unimodal size distribution, and positive zeta potential values. Encapsulation of CuSO4 in CuSO4 NCs and the physicochemical interactions between CuSO4 and pharmaceutical excipients were investigated by solid-state studies. Of note, CuSO4 NCs group exhibited higher antiproliferation efficacies, compared with bulk CuSO4, in Caco-2 (human adenocarcinoma) cells at 75 and 100 µg/mL CuSO4 concentrations (p < 0.05). Also, near-infrared laser irradiation to CuSO4 NCs group elevated the antiproliferation efficacies, compared with non-irradiation group, in Caco-2 cells. After intravenous injection in mice, CuSO4 NCs did not show severe in vivo toxicities. Developed CuSO4 NCs can be one of promising candidates of photothermal therapeutic agents for colon cancers.