RESUMO
Hypoxic damage to the prefrontal cortex (PFC) has been implicated in the frontal lobe dysfunction found in various neuropsychiatric disorders. The underlying subcortical mechanisms, however, have not been well explored. In this study, we induced a PFC-specific hypoxia-like damage by cobalt-wire implantation to demonstrate that the role of the mediodorsal thalamus (MD) is critical for the development of frontal lobe dysfunction, including frontal lobe-specific seizures and abnormal hyperactivity. Before the onset of these abnormalities, the cross talk between the MD and PFC nuclei at theta frequencies was enhanced. During the theta frequency interactions, burst spikes, known to depend on T-type Ca(2+) channels, were increased in MD neurons. In vivo knockout or knockdown of the T-type Ca(2+) channel gene (Ca(V)3.1) in the MD substantially reduced the theta frequency MD-PFC cross talk, frontal lobe-specific seizures, and locomotor hyperactivity in this model. These results suggest a two-step model of prefrontal dysfunction in which the response to a hypoxic lesion in the PFC results in abnormal thalamocortical feedback driven by thalamic T-type Ca(2+) channels, which, in turn, leads to the onset of neurological and behavioral abnormalities. This study provides valuable insights into preventing the development of neuropsychiatric disorders arising from irreversible PFC damage.
Assuntos
Canais de Cálcio Tipo T/metabolismo , Lobo Frontal/lesões , Lobo Frontal/fisiopatologia , Neurônios/metabolismo , Tálamo/metabolismo , Análise de Variância , Animais , Western Blotting , Condicionamento Clássico/fisiologia , Eletrofisiologia , Medo , Feminino , Corpos Estranhos , Lobo Frontal/metabolismo , Imuno-Histoquímica , Imageamento por Ressonância Magnética , Masculino , Camundongos , Atividade Motora/fisiologiaRESUMO
This study examined the neuroprotective effects of magnesium-sulfate (MgSO(4)) on the cerebral blood flow (CBF) and extracellular glutamate concentration in an eleven vessel occlusion (11VO) rat model. Twenty-one male Sprague-Dawley rats (250-350g) were used for the 11VO ischemic model, which was induced by a 10-min transient occlusion. The animals were divided into 3 groups, including ischemic-induced animals (ischemia group), ischemic-induced and MgSO(4) treated animals (MgSO(4) group), and sham animals for comparison. The real-time extracellular glutamate concentration was measured using a microdialysis biosensor, and the CBF was monitored by laser Doppler flowmetry. Neuronal cell death in the hippocampal region was observed 72h after ischemia by several stains (Nissl, DAPI, NeuN, and cleaved caspase3). A significant decrease in %CBF was observed in both the ischemia and MgSO(4) groups, such as ~10% during the ischemic period. However, the MgSO(4) group showed a significant decrease in the initial reperfusion %CBF compared to the ischemia group. A significantly lower level of glutamate release was observed in the MgSO(4) group than in the ischemia group during the ischemic and reperfusion episode. Our staining results revealed a significant decrease in neuronal cell death in the hippocampus in the MgSO(4) group compared to the ischemia group. These results suggest that MgSO(4) is responsible for the protection of neuronal cells by suppressing the release of extracellular glutamate under ischemic conditions and the CBF response during the initial reperfusion period.
Assuntos
Isquemia Encefálica/tratamento farmacológico , Região CA1 Hipocampal/efeitos dos fármacos , Ácido Glutâmico/metabolismo , Sulfato de Magnésio/farmacologia , Fármacos Neuroprotetores/farmacologia , Neurotoxinas/metabolismo , Células Piramidais/efeitos dos fármacos , Traumatismo por Reperfusão/prevenção & controle , Animais , Antígenos Nucleares/metabolismo , Apoptose/efeitos dos fármacos , Isquemia Encefálica/complicações , Isquemia Encefálica/fisiopatologia , Região CA1 Hipocampal/patologia , Caspase 3/metabolismo , Circulação Cerebrovascular/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Fluxometria por Laser-Doppler , Masculino , Proteínas do Tecido Nervoso/metabolismo , Células Piramidais/patologia , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/etiologia , Método Simples-CegoRESUMO
Acupuncture (ACU) is known to be effective in ischemia treatment, and glutamate (GLU) excitotoxicity is an important factor in neuronal cell death. We observed the effect of ACU on cerebral blood flow (%CBF) and DeltaGLU (the changes in GLU release) in the ischemic stroke rat model of diabetic mellitus (DM). A global ischemia was induced using the eleven-vessel occlusion (11-VO) method in 14 Sprague-Dawley rats (DM), which were randomly divided into two groups: the control group and the ACU-treatment group. Extracellular DeltaGLU was assessed using an intra-cerebral biosensor system measuring 256 samples per second, simultaneously with %CBF and electroencephalogram. ACU stimulation was applied to ACU points GB34 and GB39 during the ischemic period. Twenty-three diagnostic parameters were proposed first for a detailed analysis of changes in %CBF and GLU release during ischemia/reperfusion. ACU rats showed a significant decrease in ischemic (p < 0.05) and reperfusion %CBF (p < 0.0001) than control rats, and a significantly larger decrease in ischemic DeltaGLU (p < 0.05) and peak level of reperfusion DeltaGLU (p < 0.005) than control rats. From these results, we suggest that ACU stimulation is responsible for the potential protection of neurons through suppression of %CBF response in the increased plasma osmolality and extracellular DeltaGLU in diabetic rats under ischemic conditions.