[Changes in contents of aluminium, calcium and phosphorus contents of local bone after implantation of alumina endoprosthesis].

Thirty rabbits were divided equally into 5 groups randomly. A hole, 6mm in diameter and 2mm deep, was bored on both ilia of the thirty animals. Two cubes of alumina were implanted into the hole on one side of each animal, while the hole on the opposite side served as control. Six rabbits each were sacrificed on 10, 20, 40, 60 and 90 days after operation. Calcium, phosphorus and aluminium contents of bone tissues in and around both holes were determined by Inductively Coupled Plasma-Atomic Emission Spectrometry. The results show that the aluminium content of the bone tissues on the implanted side in each group was higher than that of the control with statistically significant difference in the 10-, 40- and 60-day groups (P < 0.05). This means that the implantation definitely releases aluminium into the bone tissues. On the contrary, calcium and phosphorus contents were significantly lower in the bone tissues on the implanted side than in the control side in the 10- and 20-day groups (P < 0.05-0.001). It implies that the aluminium released from the implant in the early stage can interfere with the metabolism of calcium and phosphorus and thus delay the mineralization of the bone tissues.

Local changes in aluminium, calcium and phosphorus content of bone caused by alumina implant.

Thirty rabbits were divided equally into 5 groups randomly. A hole, 6 mm in diameter and 2 mm deep, was bored on each iliac crest. Two pieces of alumina were implanted into the hole of one side, while the opposite side served as control. These rabbits were killed on 10, 20, 40, 60 and 90 days after operation. Calcium, phosphorus and aluminium contents of iliac bone on both sides were determined by Inductively Coupled Plasma--Atomic Emission Spectrometry. The results showed that the aluminium content of the implanted side in each group was higher than that of the control and difference was significant in 10, 40 and 60 day groups (P < 0.05). This shows that the implant releases aluminium into the bone. Moreover, the calcium and phosphorus contents were significantly lower on the implanted side than on the control side in 10 and 20 day groups (P < 0.05-0.001). Apparently, the aluminium released from the implant in the early stage can interfere with the local calcium and phosphorus metabolism and delay the mineralization of the bone.

[Histochemical study on effect of radix Salviae miltiorrhizae on growth of isolated cells from embryonic chicken frontal bone cultured in vitro].

12-day embryonic chicken frontal bone digested with trypsin to prepare the suspension of isolated bone cells. 3 x 10(6) cells were harvested altogether. The cells were divided equally into five parts. Then the Eagle medium and 0.1%, 0.2%, 0.4% and 0.8% Radix Salviae Miltiorrhizae in Eagle medium were added respectively and cultured in 5% CO2 incubator. It was observed under the inverted microscope every day. At the 26th day of culture, the cells were studied. The specimens were stained with H. E., Alcian Blue-Sirius Red, Alizarin Red S staining and alkaline phosphatase-acid phosphatase reaction for comparison. It was found that the maturation of the osteoblast-like cells could be accelerated by Radix Salviae Miltiorrhizae. Secretion of the collagenous substance, positive alkaline phosphatase reaction and deposition of mineral on the collagenous substance, forming bone nodules were found to be enhanced. But unduly high concentration of Radix Salviae Miltiorrhizae could lead to inhibition of osteoblast-like cell growth. The optimal concentration of Radix Salviae Miltiorrhizae was 0.2% in culture medium.