RESUMO
Two new fatty abietane diterpenoids, oleoyl neocryptotanshinone (1) and oleoyl danshenxinkun A (2), were isolated as minor components from the roots of Salvia miltiorrhiza. Their structures were established on the basis of spectral evidence. In preliminary tests, they selectively inhibited rabbit platelet aggregation induced by arachidonic acid.
Assuntos
Diterpenos/química , Ácidos Oleicos/química , Plantas Medicinais/química , Inibidores da Agregação Plaquetária/química , Animais , China , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Hidrólise , Técnicas In Vitro , Espectroscopia de Ressonância Magnética , Ácidos Oleicos/isolamento & purificação , Ácidos Oleicos/farmacologia , Raízes de Plantas/química , Agregação Plaquetária/efeitos dos fármacos , Inibidores da Agregação Plaquetária/isolamento & purificação , Inibidores da Agregação Plaquetária/farmacologia , Coelhos , Espectrofotometria UltravioletaRESUMO
The abietane diterpenoid, neocryptotanshinone II, and the known 6,12-dihydroxyabieta-5,8,11,13-tetraen-7-one were isolated as minor components from the roots of Salvia miltiorrhiza. Their structures were established on the basis of spectral evidence.
Assuntos
Abietanos , Diterpenos/química , Diterpenos/isolamento & purificação , Plantas Medicinais/química , Raízes de Plantas/químicaRESUMO
Fifteen tanshinone analogues isolated from the chloroform extract of Danshen roots (Salviae Miltiorrhizae Radix) by chromatographic procedures were tested for their cytotoxic activities against KB, Hela, Colo-205 and Hep-2 carcinoma cell lines. Several of them were effective at concentrations below 1 micrograms/ml concentrations. Tanshinone analogues with either hydroxy substitutions or olefinic feature in ring A demonstrated higher biologic activities. Analysis of structure-activity relationship indicate that the basic requirement for activity is the presence of a furano-o-naphthoquinone in the molecule. Compounds which lack an intact furan ring were found to be inactive. It is suggested that the planar phenanthrene ring of the tanshinones may be essential for interaction with DNA molecule whereas the furano-o-quinone moiety could be responsible for the production of reactive free radicals in the close vicinity of the bases to cause DNA damage.
Assuntos
Anti-Infecciosos/farmacologia , Fenantrenos/farmacologia , Células Tumorais Cultivadas/efeitos dos fármacos , Abietanos , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/química , Carcinoma , Neoplasias do Colo , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Células KB , Neoplasias Laríngeas , Dose Letal Mediana , Fenantrenos/administração & dosagem , Fenantrenos/químicaRESUMO
Two new diterpenoids, designated neocryptotanshinone and isotanshinone IIB, have been isolated from "Tan-Shen," the root of Salvia miltiorrhiza, together with a known compound, danshexinkun A. Their structures are established by spectral and physical data. Isotanshinone IIB exhibits significant inhibitory activity in vitro on ADP- and collagen-induced aggregation.