Mechanism Study on Chinese Medicine in Treatment of Nodular Goiter / 中国结合医学杂志

Nodular goiter has become increasingly prevalent in recent years. Clinically, there has been a burgeoning interest in nodular goiter due to the risk of progression to thyroid cancer. This review aims to provide a comprehensive summary of the mechanisms underlying the therapeutic effect of Chinese medicine (CM) in nodular goiter. Articles were systematically retrieved from databases, including PubMed, Web of Science and China National Knowledge Infrastructure. New evidence showed that CM exhibited multi-pathway and multi-target characteristics in the treatment of nodular goiter, involving hypothalamus-pituitary-thyroid axis, oxidative stress, blood rheology, cell proliferation, apoptosis, and autophagy, especially inhibition of cell proliferation and promotion of cell apoptosis, involving multiple signal pathways and a variety of cytokines. This review provides a scientific basis for the therapeutic use of CM against nodular goiter. Nonetheless, future studies are warranted to identify more regulatory genes and pathways to provide new approaches for the treatment of nodular goiter.

Effect of nitrogen,phosphorus and potassium formula fertilizer on growth and chemical composition content of Inula japonica / 中国中药杂志

Inula japonica was used as the research object, "3414" fertilization experiment were conducted to study the effects of nitrogen,phosphorus and potassium formula fertilizer on the growth and chemical composition content of I. japonica. The characteristics of fertilizer requirement were preliminarily revealed and the study provided fertilization guidance for artificial cultivation of I. japonica. The results showed that different nitrogen,phosphorus and potassium formula fertilizers had significant effects on plant morphology,physiological and biochemical indexes,dry matter accumulation and chemical composition content. The growth indexes and chemical components of I. japonica showed an upward trend with the increase of fertilization amount,especially the nitrogen fertilizer was the most significant. The indicators were analyzed by membership function. After comprehensive evaluation,the optimal nitrogen,phosphorus and potassium formula fertilization level was N3 P2 K2,namely high level nitrogen fertilizer,medium level phosphorus fertilizer and potassium fertilizer. I. japonica is a high fertilizer demand plant,and the rational fertilization scheme is " applying nitrogen fertilizer again and applying phosphorus and potassium fertilizer properly".

Analysis on character of mineral elements in Changium smyrnioides and rhizosphere soil / 中国中药杂志

The contents of 22 kinds of mineral elements in different parts of Changium smyrnioides and in the rhizosphere soil of 10 different populations were determined by microwave digestion-inductively coupled plasma atomic emission spectrometry (ICP-AES). The characteristics of mineral elements in the plants and the soil of main distribution area of Ch. smyrnioides was analyzed and the mechanism of the quality formation of Ch. smyrnioides was explored to provide the basis for the quality evaluation and cultivation regulation of Ch. smyrnioides.The results showed that the quality of soil environment was better in the main distribution area of Ch. smyrnioides, the content of trace elements was higher, the contents of P and K were lower and the contents of mineral elements in the soil of each distribution area was significantly different. The three elements of Se, P and K are significantly accumulated in the root of Ch. smyrnioides. There were significant differences in the total contents of mineral elements in the roots of Ch. smyrnioides in different producing areas. The contents of mineral elements in different parts of Ch. smyrnioides were significantly different. Ch.smyrnioides of the main distribution area of Ch. smyrnioides belonged to the safety level, the distribution of mineral elements in the plants can be used as an indicator of the quality of medicinal herbs.

Quality classification criteria of Paeonia suffruticosa seeds / 中国中药杂志

In order to establish the quality classification criteria of Paeonia suffruticosa seeds, thirty-one batches of P. suffruticosa seeds from different provenances were selected. The seed rooting rate, seed germination rate, seed purity, seed viability, 1,000-seed weight and moisture content were determined and analyzed through SPSS 20.0 software. Seed rooting rate, seed germination rate and seed purity were selected as the main index for classification, while 1,000-seed weight, seed viability and moisture content could be used as important references. The seed quality grading of P. suffruticosa was set as three grades. The seed quality of each grade should meet following requirements: For the first grade seeds, seed rooting rate ≥ 80%, seed germination rate ≥ 80%, seed purity ≥ 90%, seed viability ≥ 80%, 1,000-seed weight ≥ 250 g, moisture content, ≤ 10. For the second grade seeds, seed rooting rate ≥ 50%, seed germination rate ≥ 60%, seed purity ≥ 70%, seed viability ≥ 75%, 1,000-seed weight ≥ 225 g, moisture content ≤ 10. For the third grade seeds, seed rooting rate ≥ 20%, seed germination rate ≥ 45%, seed purity ≥ 60%, seed viability ≥ 45%, 1,000-seed weight ≥ 205 g, moisture content ≤ 10. The quality classification criteria of P. suffruticosa seeds have been initially established.

Retrospect and prospect of medicinal plants cultivation in China / 中国中药杂志

There is time-honored history and culture of medicinal plant cultivation in China. In the present review, the medicinal plant cultivation history in china was summarized, its current situation and question were analyzed, and the prospects of medicinal plant cultivation research were pointed out, with the purpose of accelerating the growth of medicinal plant cultivation research.

Investigation on biologically characterst of wild species and herbal quality of Changium smyrnioides / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the species resources of Changium smyrnioides.</p><p><b>METHOD</b>Nine habitat populations of C. smyrnioides, included with population environment, time limit, morphological characters and so on were investigated. The phylogenetic relationship of different habitat populations of C. smyrnioides was studied by isoenzyme electrophoretic analysis of the EST.</p><p><b>RESULT</b>The results showed that wild resources of C. smyrnioides were endangered. Seed germinating rate of cultivated plant was significantly higher than that of wild seed, but polysaccharide content in the root of wild species was significantly higher than that of cultivated plant. There existed a geographic phylogenetic relationship among different habitat populations.</p><p><b>CONCLUSION</b>The wild C. smyrnioides resources are endangered. The wild C. smyrnioides around Nanjing city are better than the others, it could be used for the further selecting of species resources.</p>

Effect of seedling and fertilizering on yield and quality of cultivated Changium smyrnioides / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of seedling and fertilizer on yield and quality of cultivated Changium smyrnioides and build a basis for its GAP.</p><p><b>METHOD</b>Experiments were conducted to study the effects of seedling quality and different fertilizering measures on yield and quality of cultivated C. smyrnioides.</p><p><b>RESULT</b>The seedling and fertilizering depth affected the yield of roots obviously. Organic fertilizer combined with nitrogen and phosphorus could increase the amylose content obviously.</p><p><b>CONCLUSION</b>In cultivation, the seedling (root length > 5.0 cm, root diameter > 0.3 cm, fresh root weight > 0.3 g) is optimal. The ideal combination is nitrogen, phosphorus and organic fertilizer. The suggested fertilizering depth ranged from 10 to 15 cm.</p>

Study on seed quality test and quality standard of Changium smyrnioides / 中国中药杂志

<p><b>OBJECTIVE</b>To study seed quality test methods, and provide basis for establishing seed quality standard of Changium smyrnioides.</p><p><b>METHOD</b>According to the seed testing regulations, the seed quality of C. smyrnioides from different producing area and in different growing years was measured.</p><p><b>RESULT AND CONCLUSION</b>The range of seed purity, weight per 1000 seeds, seed moisture content, seed viability were determined and the primary seed quality classification standard was established.</p>

RAPD analysis for genetic diversity of Chrysanthemum morifolium / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the genetic diversity of Chrysanthemum morifolium at the level of molecular biology.</p><p><b>METHOD</b>The total genomic DNA was extracted from medicinal chrysanthemums by 2% CTAB method. And the genetic diversity of 22 C. morifolium accessions was tested by RAPD marks. The NTSYS software was used to analyze the marks.</p><p><b>RESULT</b>26 10-mer arbitrary primers were found to acquire polymophic results. A total of 233 bands were amplified, of which 89.7% bands were found to be polymophic. 8.04 polymophic bands were amplified by each primer on the average. The results of cluster analysis by using UPGMA method showed that all the tested accessions could be differentiated by RAPD marks.</p><p><b>CONCLUSION</b>There actually existed much genetic diversity at the molecular level among the germplasm resources of C. morifolium. RAPD marks could be effective tools to construct DNA fingerprintings of C. morifolium. The differences between the tested chrysanthemums are related to the environments. However, it was affected by genetic facters more significantly.</p>

Study on seed dormancy mechanisms of Changium smyrnioides and its geminating conditions / 中国中药杂志

<p><b>OBJECTIVE</b>To study the seed dormancy mechanisms and the optimal seed germinating conditions, so as to provide basis for the seed testing of Changium smyrnioides.</p><p><b>METHOD</b>Put the seeds into wettish sand under temperature of 25, 20, 15, 10, 5 degrees C, observe the shape changing of embryo and measure its length every other 10 days. Carry on experiments of seed germination under temperature of 25,20, 15, 10, 5 degrees C on the paper bed and sand bed, determine the time of germination starting, time of germination lasting, germination rate and germination energy.</p><p><b>RESULT</b>Embryo grew the most quickly under 15 degrees C. The seed germination was largely prohibited when the temperature is higher than 20 degrees C. The mildew rate was very high under 25, 20, 15, 10 degrees C. Put the seeds under 5 degrees C for 45 days could quickly break off its dormancy, and its mildew rate was lower. The seeds germinate fastest under the condition of 15-10 degrees C on the paper bed, and its germination rate was the highest.</p><p><b>CONCLUSION</b>The morphological post-maturation seems the major dormancy mechanism of C. smyrnioides. Temperature is a major factor that affect seed germination of C. smymioides. The best pretreatment method is to put the seeds into 5 degrees C of wettish sand for 45 days. The best germination bed is the paper bed, and the best germination temperature is 15-10 degrees C.</p>

Postoperative intussusception in children: a review of 14 cases / 中国医学科学杂志(英文版)

<p><b>OBJECTIVE</b>To search the etiologic factor, clinical diagnosis points and treatment of postoperative intussusception (PI).</p><p><b>METHODS</b>To retrospectively review the clinical materials of 14 cases with PI including the cause of disease and treatment.</p><p><b>RESULTS</b>PI occurred within 10 days (average 4 days) after the primary operation. Bowel obstructive symptoms gradually emerged. One case was diagnosed with intussusception by sonography and received enema reduction of intussusception by hydrostatic pressure. Thirteen cases were performed secondary operation. Small intestine was main site of intussusception. Manual reduction of the lesion was performed in 12 cases and bowel resection and anastomosis was done in 1 case with bowel necrosis.</p><p><b>CONCLUSION</b>PI should be suspected if child presents with the symptoms of ileus in early postoperative period. Abdominal sonography may have some value on diagnosis of PI. Operation is the first choice for the treatment of PI.</p>

Study on inoculation technology of Cistanche tubulosa in the field / 中国中药杂志

<p><b>OBJECTIVE</b>To increase inoculation rate of Cistanche tubulosa in the field by studying inoculation technologies.</p><p><b>METHOD</b>Root-tube inoculation methed was used on field experiments. Inoculation rate of C. tubulosa was compared to different size seeds and inoculation mediums and inoculation time.</p><p><b>RESULT AND CONCLUSION</b>May is suitable inoculation time. The inoculation rate of C. tubulosa is 92.5% while the seed width is more than 0.7 mm and coarse sand is selected during inoculation period.</p>