Colonial Microcystis' biomass affects its shift to diatom aggregates under aeration mixing.

The effect of hydrodynamic mixing on controlling Microcystis blooms or changing the algal community to diatom dominance has been widely studied; however, the effects of colonial Microcystis biomass on the development of the algal community are poorly known. Here, in order to study the changes in Microcystis blooms under continuous aeration mixing, an experiment was carried out in a greenhouse with factors of varying biomass of Microcystis and inorganic nitrogen and phosphorus enrichment in summer. There were three chlorophyll a (Chl-a) levels in six treatments: low Chl-a level of 68.4 µg L-1 (treatments L, L-E), medium Chl-a level of 468.7 µg L-1 (treatments M, M-E), and high Chl-a level of 924.1 µg L-1 (treatments H, H-E). Treatments L-E, M-E and H-E were enriched with the same inorganic nitrogen and phosphorus nutrients. During the experiment of 30 days, the concentration of Microcystis and Chl-a decreased, and diatom Nitzschia palea cells appeared in all the treatments, which became dominant in treatments M, M-E, H and H-E, with the highest biomass of 9.41 ± 1.96 mg L-1 Nitzschia in treatment H-E on day 30. The rank order of the biomass of Nitzschia from low to high was (L = L-E) < (M = M-E) < H < H-E (P < 0.05). In addition, Nitzschia cells were aggregates attached to Microcystis colonies in all the treatments. The results showed that the initial biomass of colonial Microcystis affected the algal shift from Microcystis dominance to Nitzschia dominance. However, the enriched inorganic nitrogen and phosphorus was beneficial for the Nitzschia increase in the high biomass treatment alone. The shift from Microcystis dominance to diatom dominance under continuous aeration mixing may be caused by low light conditions as well as the nutrients released from Microcystis decay. Moreover, the aerobic condition caused by aeration mixing maintained the colonial mucilaginous sheath to support the growth of Nitzschia cells in aggregation. This study found for the first time that Microcystis blooms could shift to diatom Nitzschia dominance in aggregates. It provided a method to control and manipulate Microcystis blooms to diatom dominance through continuous aeration mixing to proper biomass of Microcystis colonies. The shift to diatoms dominance would provide more high quality food organisms for aquaculture and be beneficial to the material cycling and energy flowing in food web dynamics.

Effects of dietary melatonin on antioxidant and immune function of the Pacific white shrimp (Litopenaeus vannamei), as determined by transcriptomic analysis.

Melatonin (MT) is regarded as an antioxidant and immunostimulant that can efficiently scavenge free radicals and activate antioxidant enzymes. The aim of this study was to investigate the effects of dietary MT on the growth performance and immune function of the Pacific white shrimp (Litopenaeus vannamei). Six groups of L. vannamei were supplemented with dietary MT at 0, 22.5, 41.2, 82.7, 165.1, and 329.2 mg/kg levels for 2 months. RNA-Seq analysis was performed to obtain transcriptome data of the control group and the group supplemented with dietary MT at 82.7 mg/kg BW. In total, 1220 DEGs (799 up-regulated and 421 down-regulated) were identified. Pathways and genes related to growth performance and immune function were verified by real-time quantitative polymerase chain reaction. The total hemocyte count, phagocytosis rate, and respiratory burst were significantly increased in the MT (82.7 mg/kg BW) group as compared to the control group. Analysis of antioxidant-related enzymes in the hepatopancreas showed that dietary MT (82.7 mg/kg BW) significantly increased activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase, while dietary MT at 41.2 mg/kg BW significantly increased activities of glutathione S-transferase, lysozyme (LZM), and phenoloxidase (PO). At the transcriptional level, dietary MT up-regulated expression levels of genes associated with antioxidant immunity and growth, which included PO, SOD, LZM, GPx, chitin synthase, ecdysone receptor, calcium-calmodulin dependent protein kinase I, and retinoid X receptor. In conclusion, dietary MT may improve the growth performance and immune function of L. vannamei to some extent.

Transcriptomic analysis of the antioxidant responses and immunomodulatory effects of dietary melatonin in red swamp crayfish (Procambarus clarkii).

This study aimed to investigate the effects of dietary melatonin (MT) levels on the antioxidant capacity, immunomodulatory, and transcriptional regulation of red swamp crayfish. Six experimental diets with different levels of MT (0, 22.5, 41.2, 82.7, 165.1, and 329.2 mg/kg diet) were fed to juvenile crayfish for 60 d. The transcriptome data of the control group and the group supplemented with dietary MT at 165.1 mg/kg were obtained using RNA-seq. In total, 3653 differentially expressed genes (2082 up-regulated and 1571 down-regulated) were identified. Pathways and genes related to antioxidant immune and growth performance were verified by qRT-PCR. The total hemocyte count, phagocytosis rate, and respiratory burst were significantly increased in the MT (165.1 mg/kg) group compared to the control group. Analysis of antioxidant immune-related enzymes in the hepatopancreas demonstrated that dietary MT (165.1 mg/kg) significantly increased activities of catalase, superoxide dismutase, glutathione reductase, and glutathione peroxidase and significantly decreased aspartate aminotransferase and alanine aminotransferase activity. At the transcriptional level, dietary MT up-regulated expression levels of genes associated with antioxidant immune and development, which included toll-like receptors, Crustin, C-type lectin, and so on. To conclude, MT could be used as a supplement in crayfish feed to increase immunity and antioxidant capacity and according to the broken line regression, the ideal MT concentration was the 159.02 mg/kg. Overall, this study demonstrates the role of melatonin in the antioxidant responses and immunomodulatory of Procambarus clarkii, laying the foundation for the development of melatonin as a feed additive in the aquaculture of this species.