Assuntos
Enterobacteriáceas Resistentes a Carbapenêmicos/isolamento & purificação , Infecção Hospitalar/terapia , Infecções por Enterobacteriaceae/terapia , Controle de Infecções/métodos , Enterococos Resistentes à Vancomicina/isolamento & purificação , Idoso , Roupas de Cama, Mesa e Banho/microbiologia , Clorexidina/administração & dosagem , Protocolos Clínicos , Vestuário , Infecção Hospitalar/microbiologia , Infecção Hospitalar/transmissão , Enema/métodos , Infecções por Enterobacteriaceae/microbiologia , Infecções por Enterobacteriaceae/transmissão , Microbiologia Ambiental , Transplante de Microbiota Fecal , Feminino , Microbioma Gastrointestinal , Humanos , Higiene , Lactobacillus/metabolismo , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Quarentena , Pele/microbiologia , Resultado do TratamentoRESUMO
Viscum album coloratum (Korean mistletoe) is a semi-parasitic plant that grows on various trees and has a variety of biological functions such as immunomodulation, apoptosis, and anti-tumor activity. In this study, we investigated the effects of Korean mistletoe extract (KME) on lifespan in experimental models using Caenorhabditis elegans and Drosophila melanogaster. Supplementation of KME at 50 µg/ml extended the mean survival time by 9.61 and 19.86 % in worms and flies, respectively. The longevity benefit of KME was not due to reduced feeding, reproduction, and/or locomotion in flies and worms. The supplementation of KME also did not increase resistance to various stresses including heat shock, oxidative, or starvation stresses. Furthermore, KME did not further extend the lifespan of flies fed a dietary restricted diet but did increase the expression of Sir2, one of the target genes of dietary restriction, suggesting that KME may function as a putative dietary restriction mimetic. These results also suggest that the longevity promoting effects of KME may be an example of mild stress-induced hormesis.
Assuntos
Caenorhabditis elegans/efeitos dos fármacos , Caenorhabditis elegans/fisiologia , Drosophila melanogaster/efeitos dos fármacos , Longevidade/efeitos dos fármacos , Viscum album/química , Animais , Restrição Calórica , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Drosophila melanogaster/fisiologia , Feminino , Expressão Gênica/efeitos dos fármacos , Genes de Insetos , Histona Desacetilases/genética , Masculino , Medicina Tradicional Coreana , Extratos Vegetais/farmacologia , Sirtuínas/genética , Estresse Fisiológico/efeitos dos fármacosRESUMO
Arabidopsis thaliana infected with Beet severe curly top virus (BSCTV) exhibits systemic symptoms such as stunting of plant growth, callus induction on shoot tips, and curling of leaves and shoot tips. The regulation of sucrose metabolism is essential for obtaining the energy required for viral replication and the development of symptoms in BSCTV-infected A. thaliana. We evaluated the changed transcript level and enzyme activity of invertases in the inflorescence stems of BSCTV-infected A. thaliana. These results were consistent with the increased pattern of ribulose-1,5-bisphosphate carboxylase/oxygenase activity and photosynthetic pigment concentration in virus-infected plants to supply more energy for BSCTV multiplication. The altered gene expression of invertases during symptom development was functionally correlated with the differential expression patterns of D-type cyclins, E2F isoforms, and invertase-related genes. Taken together, our results indicate that sucrose sensing by BSCTV infection may regulate the expression of sucrose metabolism and result in the subsequent development of viral symptoms in relation with activation of cell cycle regulation.
Assuntos
Arabidopsis/enzimologia , Geminiviridae/fisiologia , Regulação Enzimológica da Expressão Gênica , Doenças das Plantas/virologia , beta-Frutofuranosidase/genética , Arabidopsis/genética , Arabidopsis/virologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Sequência de Bases , Beta vulgaris/virologia , Carotenoides/metabolismo , Proteínas de Ciclo Celular/genética , Clorofila/metabolismo , Ciclinas/genética , Fatores de Transcrição E2F/genética , Regulação da Expressão Gênica de Plantas , Glucosiltransferases/metabolismo , Inflorescência/enzimologia , Inflorescência/genética , Inflorescência/virologia , Dados de Sequência Molecular , Folhas de Planta/enzimologia , Folhas de Planta/genética , Folhas de Planta/virologia , Caules de Planta/enzimologia , Caules de Planta/genética , Caules de Planta/virologia , Plantas Geneticamente Modificadas , Ribulose-Bifosfato Carboxilase/metabolismo , Sacarose/metabolismo , beta-Frutofuranosidase/metabolismoRESUMO
Intestinal pseudo-obstruction is a massive colonic dilation with signs and symptoms of colonic obstruction, but without a mechanical cause. A 49-year-old female patient complained of nausea, vomiting, and abdominal distension 1 month after a massive brainstem hemorrhage. No improvement was seen with conservative treatments. An extended-length rectal tube was inserted to perform glycerin enema. In addition, bethanechol (35 mg per day) was administered to stimulate colonic motility. The patient's condition gradually improved over a 2-month period without any surgical intervention. Extended length rectal tube enema and bethanechol can be used to improve intestinal pseudo-obstruction in stroke patients.