RESUMO
In the current study, we show the anti-oxidative and hypocholesterol effects of aloe vera in the liver. Male specific pathogen-free (SPF) Fischer 344 rats were randomly assigned to one of four groups: Group A (control) was fed test chow without aloe supplementation; Group B was fed a diet containing a 1% (per weight basis) freeze-dried aloe filet; Group C was fed a diet containing a 1% (per weight basis) charcoal-processed, freeze-dried aloe filet; and Group D was fed a diet containing a charcoal-processed freeze-dried, whole leaf aloe (0.02% per weight basis) in the drinking water. Our results show that a life-long intake of aloe had superior anti-oxidative action against lipid peroxidation in vivo, as indicated by reduced levels of hepatic phosphatidylcholine hydroperoxide. Additional anti-oxidative action was evidenced by enhanced superoxide dismutase (SOD) and catalase activity in groups B and C. Furthermore, our study revealed that hepatic cholesterol significantly increased in the control group during aging in contrast to the aloe-supplemented groups, which showed approximately 30% lower cholesterol levels, thereby an effective hypocholesteremic efficacy. In this report, we suggest that life-long dietary aloe supplementation suppresses free radical-induced oxidative damage and age-related increases in hepatic cholesterol.
Assuntos
Envelhecimento/metabolismo , Aloe , Anticolesterolemiantes/farmacologia , Colesterol/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/metabolismo , Envelhecimento/sangue , Aloe/química , Animais , Catalase/metabolismo , Colesterol/sangue , Cromatografia Líquida de Alta Pressão/métodos , Suplementos Nutricionais , Masculino , Fosfatidilcolinas/análise , Extratos Vegetais/farmacologia , Distribuição Aleatória , Ratos , Ratos Endogâmicos F344 , Organismos Livres de Patógenos Específicos , Superóxido Dismutase/metabolismo , Resultado do TratamentoRESUMO
The present study explores the dietary effect of pectin on the MLN lymphocyte functions of mice with dextran sulfate sodium (DS)-induced colitis. We found that the immunoglobulin (Ig)A level in mesenteric lymph node (MLN) lymphocytes was high, while the IgE level was lower, in mice fed with pectin than in those fed with cellulose. Interestingly, the fecal IgA concentration of the pectin-fed mice was significantly higher than that of the cellulose-fed mice. The concentrations of interferon-gamma and interleukin (IL)-2 treated with concanavalin A (ConA) were significantly higher in the pectin-fed group than in the cellulose-fed group. Although dietary pectin did not affect the IL-4 and IL-10 levels, the activation-induced IL-4 and IL-10 secretion was lower in MLN cells of the pectin-fed mice than of the cellulose-fed mice following DS-induced colitis. Based on these findings, we propose that the effect of dietary pectin on mice with DS-induced colitis is mediated by the manipulation of Th1 cells. Furthermore, the inhibitory effect of IL-4 and IL-10 by dietary pectin may play an important role in promoting a change in Th1/Th2 balance toward Th1-dominant immunity.
Assuntos
Colite/imunologia , Citocinas/biossíntese , Imunoglobulina A/biossíntese , Imunoglobulina E/biossíntese , Linfonodos/imunologia , Linfócitos/imunologia , Pectinas/farmacologia , Animais , Peso Corporal , Colite/induzido quimicamente , Citocinas/metabolismo , Sulfato de Dextrana , Fibras na Dieta/farmacologia , Ingestão de Alimentos , Fezes/química , Feminino , Linfonodos/citologia , Linfonodos/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Mesentério/efeitos dos fármacos , Mesentério/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Fatores de TempoRESUMO
Effect of nicotine on nitric oxide synthase (NOS) expression in various hypothalamic regions was investigated in rats via nicotineamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry. Sprague-Dawley rats were divided into the fed group, the fed and nicotine-treated group, the food-deprived group, and the food-deprived and nicotine-treated group. The fed groups received abundant food and water, while food was withheld from the food-deprived groups for 48 h. The nicotine-treated groups were injected with nicotine. Following food deprivation, enhanced NAPDH-d expression was detected in the paraventricular nucleus, ventromedial hypothalamic nucleus, and lateral hypothalamic area of the hypothalamus. Nicotine administration to the food-deprived rats resulted in decreased NADPH-d positivity. The present results indicate that nicotine administration is effective in limiting the enhancement in NOS expression following food restriction.