Valorization of spent coffee grounds through integrated bioprocess of fermentable sugars, volatile fatty acids, yeast-based single-cell protein and biofuels production.

Recovering nutrients from waste for biological processes aligns with sustainability principles. This study aimed to convert spent coffee grounds (SCG) into valuable products, including fermentable sugars, volatile fatty acids (VFAs), yeast-based single-cell protein and biofuels. Alkaline pretreatment was conducted before enzymatic hydrolysis, in which the pretreated SCG was hydrolyzed with varying enzyme loadings (20-60 filter paper units (FPU)/g-solid) and solid loadings (3-15 % w/v). The hydrolyzed slurry was utilized for VFAs and hydrogen production, yielding high values of 0.66 g/g-volatile solids (VS) and 109 mL/g-VS, respectively, using an enzyme loading of 50 FPU/g-solid and a solid loading of 3 % (w/v). The derived VFAs were used to cultivate a newly isolated yeast, Candida maltosa KKU-ARY2, resulting in an accumulated protein content of 43.7 % and a biomass concentration of 4.6 g/L. This study highlights the conversion of SCG into essential components, emphasizing the benefits of waste utilization through cascade bioprocesses.

Ameliorating Effect on Aß-Induced Alzheimer's Mice by Litsea cubeba Persoon Powder.

Alzheimer's disease (AD) is caused by excessive oxidative damage and aging. The objective of this study was to investigate the anti-dementia effect of LCP fruit powder on amyloid ß (Aß)-induced Alzheimer's mice. The composition of LCP essential oil was determined by gas chromatography/mass spectrometry. In addition, the water maze was used to evaluate the learning and memorizing abilities of the mice. The concentrations of malondialdehyde (MDA), protein carbonyl, phosphorylated τ-protein, and the deposition of Aß plaques in mouse brains were also assessed. The results showed that the main components of essential oils in LCP and d-limonene, neral, and geranial contents were 14.15%, 30.94%, and 31.74%, respectively. Furthermore, oral administration with different dosages of LCP significantly decreased the escape time (21.25~33.62 s) and distance (3.23~5.07 m) in the reference memory test, and increased the duration time (26.14~28.90 s) and crossing frequency (7.00~7.88 times) in the target zone of probe test (p < 0.05). LCP also inhibited the contents of MDA and the phosphor-τ-protein from oxidative stress, reduced the brain atrophy by about 3~8%, and decreased the percentage of Aß plaques from 0.44 to 0.05%. Finally, it was observed that the minimum dosage of LCP fruit powder (LLCP, 30.2 mg/day) could prevent oxidative stress induced by Aß and subsequently facilitate memory and learning deficits in Aß-induced neurotoxicity and cognitively impaired mice.

Active food ingredients production from cold pressed processing residues of Camellia oleifera and Camellia sinensis seeds for regulation of blood pressure and vascular function.

Hypertension is one of the most common illnesses worldwide. Accurate control of blood pressure can help reduce the incidence of complications. Nω-nitro-l-arginine methyl ester (l-NAME) is a nitric oxide synthase inhibitor that increases oxidative stress and inflammatory responses, activating the expression of transforming growth factor-beta (TGF-ß), which thickens the vessel wall and ultimately contributes to hypertension. Studies have shown that seeds of Camellia oleifera Abel and Camellia sinensis (L). O. Kuntze (Oolong tea) possesses antibacterial, antioxidant, and anti-inflammatory functions. Therefore, this study aimed was to investigate the functional components in the seed pomace ethanol extracts of C. oleifera Abel (CPE) and Oolong tea (OPE) and to evaluate the ameliorative effects of CPE and OPE on oxidative stress, inflammation, and vascular remodeling in l-NAME induced hypertensive C57BL/6J mice. After 8 weeks of treatment, all CPE and OPE dose groups significantly reduced systolic and diastolic blood pressure, by over 30 mmHg and 15 mmHg, respectively. Additionally, CPE and OPE decreased transforming growth factor-beta (TGF-ß) expression in the thoracic aortic and thoracic aortic intima-media thickness. Moreover, CPE and OPE decreased the malondialdehyde concentration in the liver by over 33%, as well as levels of tumor necrosis factor-α, interleukin 6, and interleukin-1ß in the kidney and heart. Collectively, CPE and OPE can reduce oxidative stress and vascular remodeling, lowering blood pressure, and reducing the risk of cardiovascular disease.

Role of Camellia brevistyla (Hayata) Coh. Stuart Seed Pomace Extract on Hypertension and Vascular Function in L-NAME-Treated Mice.

Camellia brevistyla (Hayata) Coh. Stuart seeds are used to produce edible oil. The seed pomace is an agricultural waste, containing approximately 8% saponin, which has antihypertensive effects. Nω -nitro-L-arginine methyl ester (L-NAME) can induce hypertension with no deficiency on mice. Here, we investigated the effects of ethanol extract from C. brevistyla seed pomace (CBPE) in L-NAME-induced hypertension mice. The results showed that all doses of CBPE significantly decreased systolic (117 ± 5-122 ± 5 mmHg) and diastolic (72 ± 16-77 ± 8 mmHg) blood pressure, aortic intima media thickness (48 ± 5-53 ± 5 µm), and also reduced the MDA adduct and protein carbonyl levels in the liver (101 ± 19-114 ± 17 ρmol/mL and 4.8 - 5.2 nmol/mg) compared to those observed in the L-NAME group (140 ± 3 and 95 ± 8 mmHg, 65 ± 10 µm, 145 ± 25 ρmol/mL, and 7.8 nmol/mg; P < 0.05). These results suggest that CBPE has profitable antihypertensive properties which are preventing aorta remodeling and reducing liver oxidative stress in hypertensive mice.