RESUMO
The total synthesis of 4-methylenegermine is described.
Assuntos
Cevanas/síntese química , Acetatos , Estrutura Molecular , Plantas Medicinais , EstereoisomerismoRESUMO
The use of plant oils as industrial feedstocks can often be hampered by their lack of optimization towards a particular process, as well as their development being risky; growing suitable volumes of crops to test can take up to five years. To circumvent this, we aimed to discover a method that would mimic plant oil profiles in the laboratory, and show that they exhibited similar properties to the naturally grown plant oils in a given process. Using the synthesis of polyurethanes as an example, we have synthesized six different polymers and demonstrated that plant oils will produce polymers with similar physical properties to those oils mimicked in the laboratory. The use of this mimicking process can be extended to other types of polymers to obtain a method for predicting the properties of a given material based on the plant oil composition of a crop before it is grown in bulk.
Assuntos
Óleos de Plantas/química , Poliuretanos/síntese química , Materiais Biomiméticos/síntese química , Materiais Biomiméticos/química , Cannabis/química , Ácidos Graxos/análise , Ácidos Graxos/química , Ácidos Graxos Monoinsaturados , Glicerol/química , Hidroxilação , Teste de Materiais , Estrutura Molecular , Óleos de Plantas/síntese química , Poliuretanos/química , Óleo de Brassica napus , Resistência à Tração , Triglicerídeos/síntese química , Triglicerídeos/químicaRESUMO
We prepare a biotinylated conjugate of the ubiquitous plant hormone (S)-(+)-abscisic acid via an acyl hydrazone linkage at the C4' position and demonstrate in vivo cleavage of the otherwise stable acyl hydrazone linkage using LC-MS2. As part of a wider chemical genomic study, biological activity of the conjugate was assessed using standard epidermal peel and gravimetric transpiration assays, showing significant activity but at a level lower than the unconjugated hormone. When deuterated samples of the conjugate were fed to the plant, however, it was apparent by LC-MS2 experiments that significant levels of hydrolysis of the acyl hydrazone had taken place, contrary to in vitro stability assays in artificial sap. We conclude that abscisic acid is liberated in sufficient quantities to account for the observed physiological response and that LC-MS2 monitoring of conjugates is a simple and practical method by which such events may be assessed, whether in plants or other organisms.