Assuntos
Antígenos de Plantas/imunologia , Imunoglobulina E/imunologia , Hipersensibilidade a Trigo/imunologia , Adolescente , Adulto , Alérgenos/imunologia , Criança , Pré-Escolar , Dermatite Atópica/imunologia , Humanos , Imunoglobulina E/sangue , Lactente , Poaceae/imunologia , Pólen/imunologia , Adulto JovemRESUMO
BACKGROUND: Dietary intake of wheat causes hypersensitivity reactions in patients suffering from IgE-mediated food allergy and coeliac disease. AIM: To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars. METHODS: Summer wheat was grown in a glasshouse and seeds were harvested at defined maturation stages. Mature seeds were obtained from 13 different defined cultivars. Protein extracts were prepared from different maturation stages and cultivars with a standardized procedure based on seed weight and analysed by IgE and IgA immunoblotting using sera from clinically defined patients suffering from wheat allergy or coeliac disease. RESULTS: With a few exceptions the expression of IgE- and IgA-reactive wheat antigens increased during wheat seed maturation. Wheat cultivars could be identified in which the expression of certain IgE- and IgA-reactive components was strongly reduced or not detectable. CONCLUSIONS: The expression of IgE- and IgA-reactive antigens depends on wheat seed maturation and varies in different wheat cultivars.