RESUMO
UNLABELLED: Endometrial hyperplasias are now diagnosed according to the histopathological examination. By using immunohistochemistry, the authors search for supplementary elements for the diagnosis and evaluation of the prognosis of this nosologic framework. MATERIAL AND METHODS: We investigated a number of 138 cases of endometrial hyperplasia, using immunohistochemical research. The avidine-biotin-peroxidase method revealed estrogenic and progesterone receptors, together with EGF, proliferative Ki67 and cyclin D1. RESULTS: The reaction was positive in variable percents for estrogenic receptors, especially for complex atypical hyperplasias. A positive immunoreactivity was also found for progesterone receptors and for Ki67 (in atypical hyperplasias); cyclin D1 revealed only a moderate reaction. CONCLUSIONS: Immunohistochemistry can be useful for the prognosis of the lesion, but it is not indispensable for diagnosis, while the histopathologic examination is absolutely necessary for a complete endometrial hyperplasia diagnosis.
Assuntos
Hiperplasia Endometrial/diagnóstico , Biomarcadores Tumorais/metabolismo , Ciclina D1/metabolismo , Hiperplasia Endometrial/metabolismo , Fator de Crescimento Epidérmico , Feminino , Humanos , Imuno-Histoquímica/métodos , Antígeno Ki-67/metabolismo , Prognóstico , Receptores de Estrogênio/metabolismo , Receptores de Progesterona/metabolismoAssuntos
Cárie Dentária/terapia , Materiais Dentários/uso terapêutico , Polpa Dentária/efeitos dos fármacos , Restauração Dentária Permanente , Animais , Cárie Dentária/patologia , Polpa Dentária/ultraestrutura , Cães , Avaliação Pré-Clínica de Medicamentos , Microscopia Eletrônica , Fatores de TempoRESUMO
The effect of Silymarin (Legalon) upon liver lesions was investigated using four experimental models: In acute galactosamine-hepatitis, Silymarin administration achieved protection of the liver structure (electron-microscopy included), liver cell glycogen, RNA and enzymatic activity, Galactosamine-depressed gluconeogenesis in the isolated perfused rat liver was significantly preserved by Silymarin treatment. In lead and cadmium poisoning the structural damage and histochemical and histoenzymatic changes were partly but significantly prevented. The complex noxious effects of Imuran overdoses were favourably influenced by Silymarin, without diminishing the cytostatic-immunosuppressive action of Imuran.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Flavonoides/uso terapêutico , Galactosamina/efeitos adversos , Hepatite Animal/tratamento farmacológico , Silimarina/uso terapêutico , Animais , Azatioprina/intoxicação , Intoxicação por Cádmio/tratamento farmacológico , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Hepatite Animal/induzido quimicamente , Intoxicação por Chumbo/tratamento farmacológico , Ratos , Ratos EndogâmicosRESUMO
The hepatoprotective action of Silymarin was studied in 65 male Wistar rats, prior to and following D-galactosamine intoxication. There was a marked reduction in the histological and ultrastructural changes in the nucleolus, nuclear membrane, mitochondria, granular and agranular endoplasmic reticulum and lysosomes of the liver cell and also in the Kupffer stellate cells. The reduction in glycogen and RNA loss was determined biochemically. The activities of many enzymes were kept constant (oxidoreductases, NADH2 diaphorase, G-6-phosphatase, Mg++ and K+/Na+-dependent ATPases, acid phosphatases).