Data Mining Study on Prescription Patterns of Different Dosage Forms of Chinese Herbal Medicines for Treating and Improving Immune-Inflammatory Indices in Patients with Rheumatoid Arthritis / 中国结合医学杂志

OBJECTIVE@#To explore the prescription patterns of different dosage forms of Chinese herbal medicines (CHMs) for the treatment of rheumatoid arthritis (RA) and their effects on immune-inflammatory indices.@*METHODS@#Clinical data were collected from patients with RA in 4 hospitals (3 Class A comprehensive hospitals and 1 Class B comprehensive hospital) in Anhui Province, China, from August 2012 to June 2018 via the electronic medical record gathering system. Following extraction of prescription information, each prescribed herb was quantified and standardized according to the knowledge base to establish a database of RA treatment formulae. The medical records were divided into the granules group and decoction pieces group. Core herbs and their combination patterns were obtained from the two groups of cases using Liquorice software. Changes in immune-inflammatory and hepatic and renal function indices were compared between the two groups using SPSS 23.0 software. The Aprior module of SPSS Clementine 11.1 software was applied to analyse the correlation between CHMs and improvement in indices. Finally, the ORACLE 10 g tool was used to evaluate the random walk model of the immune-inflammatory indices between the two groups.@*RESULTS@#(1) We retrospectively analysed 35,898 prescriptions for 6,829 patients with RA who received CHM treatment. There were 3,816 patients in the granules group and 3,013 in the decoction pieces group. (2) The core herbs were Pi (Spleen)-strengthening and dampness-resolving drugs, blood-activating and stasis-resolving drugs, wind/dampness-dispelling drugs and heat-clearing and detoxifying drugs. (3) Both dosage forms could improve immune-inflammatory indices in RA patients, with similar efficacy and no influence on hepatic or renal function. (4) Herba Siegesbeckiae and Oldenlandia had a stronger association with immune-inflammatory indices in the two groups. (5) The immune-inflammatory indices showed obvious improvement after treatment with granules and decoction pieces of CHMs, and there were long range correlations between the comprehensive evaluation indices and interventions.@*CONCLUSIONS@#The principal CHM treatment methods for RA in four hospitals in Anhui Province are strengthening Pi and resolving dampness, activating blood and resolving stasis, dispelling wind/dampness and clearing heat. Granules and decoction pieces of CHMs have similar efficacy in improving immune-inflammatory indices in RA patients and could be used as treatment options for RA.

Research on Anti-aging Guided by Qiluo Doctrine / 中国实验方剂学杂志

Along with increasing degree of population aging globally， senility， good health and long life have become the focus of the world. Guided by Qiluo doctrine， an essence， Qi and spirit theory is proposed as below， essence is the origin of life， Qi is the impetus of life and spirit is the embodiment of life. Based on holistic view of kidney deficiency involving the five internal organs and injuries of the five internal organs definitely affecting the kidney， a mechanism of aging is proposed as below， deficiency of kidney essence is the foundation of aging， deficiency of promordial Qi is the key of aging and physical and spiritual loss is the manifestation of aging. It provides a theoretical guidance for anti-aging study of rejuvenating the elderly and making the strong person stronger. By virtue of the experiences in kidney-tonifying medication accumulated for more than two thousand years， Bazi Bushen capsules has been developed， which has anti-aging efficacy， including tonifying kidney， replenishing essence， coordinating Yin and Yang， supplementing primordial Qi and nourishing body and spirit. Experimental researches have demonstrated that Bazi Bushen capsules can improve overall aging and systemic aging， as well as prevent and treat aging related diseases. Preliminary clinical studies demonstrate that this capsules can enhance athletic ability and improve sexual function， and is expected to become a representative Chinese patent medicine of anti-aging. This paper addresses aging and anti-aging on the basis of Qiluo doctrine， in the hope of helping prevention and treatment of aging related diseases.

Daylight photodynamic therapy for the treatment of actinic keratosis / 中华皮肤科杂志

Actinic keratosis (AK) is a kind of precancerous skin lesions related to long-term light exposure, and photodynamic therapy is one of its main treatment methods. Compared with conventional photodynamic therapy, daylight photodynamic therapy (DL-PDT) with daylight as a light source has advantages of more convenient operation, less pain and higher patient acceptability, but it is liable to be affected by weather and other factors. DL-PDT is suitable for the treatment of grade Ⅰ or Ⅱ AK on the head and face, and its efficacy may be affected by pretreatment, photosensitizers, irradiation dose, etc. This review mainly elaborates the advantages of DL-PDT and introduces its operation methods to help clinicians choose the best treatment protocol for AK.

Evaluation of effect of Hibiscus paste external application combined with traditional Chinese medicine on inflammatory indices of patients with active rheumatoid arthritis based on random walk model / 中国免疫学杂志

Objective:To evaluate the effect of Hibiscus paste external application combined with traditional Chinese medicine on the inflammatory markers in patients with active rheumatoid arthritis (RA). Methods: The patient information was collected by the data processing system of hospitalized medical records in the First Affiliated Hospital of Anhui University of traditional Chinese medicine. The medical records of patients with rheumatoid arthritis in the Department of Rheumatology from May 2012 to December 2016 were collected. Patients were divided into experimental group and control group according to whether Furong ointment was applied. The random walk model was used to evaluate the influence of Furong ointment combined with traditional Chinese medicine on inflammatory index,erythrocyte sedimentation rate ( ESR) and high sensitivity C reactive protein ( Hs-CRP) in patients with active rheumatoid arthritis. Results: A total of 4 832 patients with rheumatoid arthritis were in accordance with the requirements of the study, including 2 579 in the experimental group and 2 253 in the control group. Baseline data analysis showed that there was no significant difference in general condition, frequency of oral Chinese medicine and core prescriptions between two groups ( P＞0. 05 ) . In the experimental group,the maximum ESR of inflammation index was 968,the walking rate was 3 756,the positive growth rate of walking was 0. 257 7,the ratio was 3. 88,the random fluctuation power law value was 0. 364 2±0. 124 6,the comprehensive evaluation index in-creased by 0. 479 9,and the comprehensive evaluation index was 2 017 times. In the control group,the maximum ESR of inflammation index was 398,the number of walking steps was 2 251,the positive growth rate of walking was 0. 176 8,the ratio was 5. 66,the random fluctuation power law value was 0. 173 5±0. 128 8,the comprehensive evaluation index increased by 0. 357,and the comprehensive evaluation index was 1 115 times. In the experimental group,the maximum Hs-CRP of inflammation index was 1 523,the walking rate was 5 149,the positive growth rate of walking was 0. 295 8,the ratio was 3. 38,the random fluctuation power law value was 0. 389 5± 0. 108 1,the comprehensive evaluation index increased by 0. 537 4,and the comprehensive evaluation index was 2 834 times. In the control group,the maximum Hs-CRP of inflammation index was 809,the number of walking steps was 3 463,the positive growth rate of walking was 0. 233 6,the ratio was 4. 28,the random fluctuation power law value was 0. 362 9±0. 073 8,the comprehensive evaluation index increased by 0. 448 2,and the comprehensive evaluation index was 1 805 times. Conclusion: Our hospital rheumatology on the treatment of rheumatoid arthritis from the spleen, oral Chinese medicine prescription to Jianpihuashi drugs, Huoxue Tongluo drugs, Qufengchushi drugs,drug detoxification,two groups of patients with comprehensive evaluation index and acceptable interventions are long-range correlation,a better effect than that of inflammatory markers associated with traditional Chinese medicine in patients with Furong ointment the simple use of Chinese herbs.

Discussion on Traditional Chinese Medicine Prescription Rule in Ankylosing Spondylitis Treatment Based on Data Mining / 世界科学技术-中医药现代化

This study was aimed to explore traditional Chinese medicine (TCM) compatibility patterns or rules in the treatment of ankylosing spondylitis (AS) in the real world clinical situations.The effect of TCM treatment and immune inflammatory biomarkers were analyzed by association rules,which provided evidences for AS syndrome differentiation and treatment.Retrospective study was conducted on records of hospitalized AS patients at the Department of Rheumatism,the First Affiliated Hospital of Anhui University of Chinese Medicine from June 2012 to June 2016.The technology for complex networks was integrated to analyze the core prescription.The frequency and association rules of prescription patterns were analyzed using the SPSS 21.0 and SPSS modeler 14.2.The effect on immune inflammatory biomarkers was analyzed by association rules and random walk model.The results showed that,(1) warm,cold,and neutral herbs,sweet,bitter or pungent herbs,and with the channel tropism of spleen channel,stomach channel and liver channel are the most frequently prescribed herbs;(2) herbs with functions of tonifying and replenishing,blood-activating and stasis-resolving,qi-regulating,spleen-strengthening and dampness-resolving,wind-dampness dispelling,heatclearing are frequently prescribed,which are also interrelated and interacted on each other;(3) the top five pairs of couplet herbs with the highest confidence included Hong Hua paired with Tao Ren (98.15％),Yi Yi Ren paired with Fu Ling (95.47％),Gan Cao paired Gou Ji (94.57％),Gan Cao paired with Wei Ling Xian (94.48％),Pu Gong Ying paired Bai Hua She She Cao (94.40％);(4) Shan Yao was highly related to decreased C3,C4 and hs-CRP when compatibility with Fu Ling,Niu Xi,Ze Xie;Gou Ji was highly related to decreased IgA,IgG and IgM when compatibility with Bai Hua She She Cao,Chuan Xiong,Shah Yao.Xi Qian Cao was associated with Niu Xi when ESR and WBC decreased.Gou Ji and Du Huo were associated when SOD increased.(5) The group of internal medicine coordinated with external medicine has positive growth rate of walk and improvement index of inflammatory indexes (ESR,hs-CRP) compared to single internal medicine group.It was concluded that AS treatment in the Department of Rheumatism of our hospital was based on spleen.The treatment was focused on the regulation of cold and warm,sweet and bitter,liver and spleen.The treatment of AS is to eliminate evil factors and to reinforce healthy qi.TCM has advantages in the improvement of immune inflammatory indexes.The improvement of inflammatory indexes by the internal medicine coordinated with external medicine was better than the single use of internal medicine,which is worthy of further promotion.

Research on Traditional Chinese Medicine Prescription Rule in Acute Gouty Arthritis Treatment Based on Data Mining / 世界科学技术-中医药现代化

Traditional Chinese medicine (TCM) is widely used in gouty arthritis treatment.Based on the real world,this article studied the implicit TCM prescription pattern in order to explore TCM prescription rule in acute gouty arthritis treatment.Based on herbs recorded in the Pharmacopoeia of the People's Republic of China,TCM prescriptions of 732 hospitalized acute gouty arthritis patients at the Department of Rheumatism and Immunology,the First Affiliated Hospital of Anhui University of Chinese Medicine from June 2012 to October 2016 were collected with software system.The frequency and association rules of prescription patterns were analyzed using the SPSS Modeler 14.2 Data Mining System.Cluster analysis was conducted using SPSS 22.0 statistical software.An initial statistical analysis was carried out to categorize herbs according to their medicinal types and dosage,natures,flavors,channel tropism and functions.Based on categorization,frequencies of occurrence were computed.The results showed that,(1) warm,cold or neutral herbs,sweet or bitter herbs,and with the channel tropism of spleen channel and stomach channel are the most frequently prescribed herbs (among 732 selected medicinal formulae);(2) spleen-strengthening and dampness-eliminating,wind-dispelling and damp-removing,blood-activating and stasis-resolving,heat-clearing,deficiency-tonifying,qi-regulating herbs are frequently used;(3) correlation analysis of TCM efficacy show that three couplet medicines with the highest confidence was spleen-strengthening and dampness-eliminating herbs and heat-clearing herbs (99.24％),blood-activating and stasis-resolving herbs and wind-dispelling and damp-removing herbs (99.01％),spleen-strengthening and dampnesseliminating herbs and qi-regulating herbs (98.92％).Correlation analysis of two TCM herbs showed that three couplet medicines with the highest confidence was Hong Hua paired with Tao Ren (99.7％),Yi Yi Ren paired with Shan Ci Gu (93.04％),and Yi Yi Ren paired with Chui Pen Cao (92.67％);(4) cluster analysis classified into five types,which were based on spleen-strengthening and dampness-eliminating and in combination with blood-activating and stasis-resolving herbs,heat-clearing and detoxification,wind-dispelling and damp-removing,representing prescription compatibility characteristics.It was concluded that gouty arthritis had a close relationship with the spleen.These prescriptions focus on herbs with spleen-strengthening and dampness-resolving which seems to be the key element in the treatment process.Meanwhile,the use of spleen-strengthening and dampness-eliminating herbs,blood-activating and stasis-resolving herbs,heat-clearing herbs,wind-dispelling and damp-removing herbs is the basis of prescription patterns.

Analysis on Traditional Chinese Medicine Prescription Patterns in 3609 Patients with Osteoarthritis Based on The Real World / 世界科学技术-中医药现代化

Traditional Chinese medicine (TCM) is widely used as a complementary approach for osteoarthritis treatment.This article is based on the real world.The implicit prescription patterns behind TCM formulae were examined by association rules mining and cluster analysis,so as to explore the Chinese medicinal compatibility patterns or rules in osteoarthritis treatment.This study was based on real-world data from the hospital information system (HIS).Chinese medicinal prescriptions of hospitalized patients with osteoarthritis from June 2012 to June 2016 were collected from the Department of Rheumatism and Immunology,the First Affiliated Hospital of Anhui University of Chinese Medicine.TCM was listed according to the selected medicinal formulae and the added information was organized to establish a database.The frequency and association rules of prescription patterns were analyzed using the SPSS Modeler 14.2 Data Mining System.Cluster analysis of prescription patterns was also analyzed.An initial statistical analysis was carried out to categorize herbs according to their natures,flavors,channel tropisms and functions.Based on categorization,frequencies of occurrence were computed.At the same time,the relationship between TCM and immune and inflammatory markers was analyzed.The results showed that the main prescriptive features from the selected formulae of the mining data were that,(1) warm or cold herbs,sweet or bitter herbs,and with the channel tropism of spleen channel are the most frequently prescribed herbs in 3609 medicinal formulae;(2) herbs with blood-activating and stasis-resolving,restoring vital energy,water-eliminating and damp-removing,heat-clearing and or expelling wind-damp functions are frequently prescribed;(3) association rule analysis extracted three frequently used pairs of herbs,which are Hong Hua & Tao Ren (98.90％),Pu Gong Ying & Bai Hua She She Cao (97.88％),Fu Ling & Ze Xie (92.63％);(4) association rule between TCM and immune-inflammation index showed that the pair of Du Huo and Chen Pi was associated with increased superoxide dismutase (SOD) enzyme.The pair of Mai Ya and Dan Shen was associated with decreased high-sensitivity C-reactive protein (hs-CRP),erythrocyte sedimentation rate (ESR),complement component 3 (C3),complement component 4 (C4),immunoglobulin A (IgA) and immunoglobulin G (IgG).The pair of Mai Ya and Fu Ling was associated with decreased immunoglobulin M (IgM).It was concluded that osteoarthritis was closely related to spleen deficiency.These prescriptions focus on herbs for qi-nourishing and spleen-invigorating,blood-activating and stasis-resolving,spleen-invigorating and dampness-eliminating,heat-clearing and detoxicating which seems to be the key element in the treatment process.Meanwhile,the use of restoring vital energy herbs and spleen-strengthening herbs also forms the basis of prescription patterns.

Compatibility rules of rheumatoid arthritis treated from spleen based on HIS4000 medical records / 世界科学技术-中医药现代化

Objective:Based on the hospital information system (hospital information system,HIS) were studied in rheumatology clinical medicine data hidden behind the prescription pattern,aims to explore the treatment of rheumatoid arthritis with patterns or rules from the spleen.Methods:This study from May 2012 to December 2016 in the treatment of active rheumatoid arthritis patients as the research object in the division of Rheumatology at the First Affiliated Hospital of Anhui TCM university.The use of Office 2010,the complex network technology and IBM Clementine 14.2 SPSS Apriori software module for Chinese medicine core prescription analysis,frequency statistics,association rule analysis.Results:a total of 4 000 copies of medical records and 3 643 prescriptions of traditional Chinese medicine were included in the study,the main results are as follows:(1) the clinical medication can be divided into jianpihuashi drugs,Huoxue Huayu drugs,dispelling dampness and heat drugs in four categories,more than 60％ times the frequency of tuckahoe,liquorice,tangerine peel,coix seed,Salvia miltiorrhiza the kernel,safflower,Chinese yam,clematis root,peach kernel,core prescription:tuckahoe,liquorice,tangerine peel,Salvia miltiorrhiza,coix seed,safflower,Chinese yam,clematis root,peach kernel,dandelion;(2) drug association analysis:Poria and Oldenlandia,dandelion,Herba Siegesbeckiae,peach kernel,Chinese medicine jianpihuashi the compatibility of blood stasis,dispelling dampness and heat drugs for 20 drug combinations;(3) compare the index difference between the two groups before and after treatment,the experimental group Hs-CRP,RF,ESR,IgA,IgG and C3 compared with the control group decreased significantly after treatment,the difference was statistically significant (P＜0.05 or P＜0.01);(4) the analysis of drug and two indicators of Relevance:Jiang Banxia,Herba Siegesbeckiae and Hs-CRP,ESR decreased the incidence is higher,white atractylodes rhizome,Salvia miltiorrhiza and RF reduce the high degree of relevance,yam,dandelion and C3 to reduce the high degree of relevance,Du Zhonghe,IGA,IGG and Honghua Weilingxian to reduce the high correlation.Analysis of the three Association:Jiang Banxia,Herba Siegesbeckiae respectively with Hs-CRP,ESR,dandelion,walnuts and reduce the high degree of relevance,astragalus,salvia,licorice and RF reduce the high degree of relevance,yam,dandelion,Herba Siegesbeckiae and C3 reduce the high degree of relevance,malt,dandelion,Radix Clematidis and IGA to reduce the high degree of relevance,eucommia,safflower,licorice and IGG reduce high correlation.Conclusion:This study on the treatment of rheumatoid arthritis from the real world to the spleen,spleen wet medicine as the core,and the compatibility between blood stasis,antipyretic,rheumatism medicine prescription is the key,which can effectively improve the patients' immune inflammation and other laboratory indexes.

High-efficiency Utilization And Sustainable Development of Traditional Chinese Medicine Resource: Based on Discovery of Functional Components / 世界科学技术-中医药现代化

As national strategic resources,traditional Chinese medicine (TCM) resource is the material basis for the pharmaceutical industry and health services.The high-efficiency utilization of TCM resource is a major strategic issue that realize the resource conservation and environment friendly recycling economy,guarantee the sustainable development of medicine.But currently,on one hand,the wild TCM resource was seriously damaged and in a serious shortage of stock,the cultivation variety was degenerated,the tending of endangered medicinal materials and the development of alternative varieties were faced many problems.On the other hand,the limited resources cannot be effectively utilized,which results in the waste of resources.Therefore,two ecological restricted resources,poria cocos and Chinese gall,were studied for comprehensive utilization of resources to explore the comprehensive exploitation and utilization of TCM resource as well as the pattern of sustainable development.We suggested that TCM resource should be taken into account as a whole for comprehensive utilization.We should especially pay attention to basic research for the sustainable development of TCM resource,discovery of bioactive substance,excavation and transformation for functional components,the use of biotechnology,the product development,the formation and extension of industry chain.So as the comprehensive exploitation and utilization of TCM resources and sustainable development would be realized.

Effect of Comprehensive Chinese Medical Therapy of Chinese Herbal Medicine Fumigation, Massage and Quadriceps Exercise on Contents of Visfatin and Chemerin in Joint Fluid of Patients with Knee Osteoarthritis / 广州中医药大学学报

Objective To study the effect of comprehensive Chinese medicine therapy,including Chinese herbal medicine fumigation,massage,and quadriceps exercise,on adipokines of visfatin and chemerin content in joint fluid of patients with knee osteoarthritis (KOA),and to explore its possible therapeutic mechanism for KOA.Methods A total of 60 cases of KOA patients were randomly divided into treatment group and control group,30 cases in each group.The treatment group was treated with comprehensive Chinese medicine therapy,and the control group was treated with Chinese medicine fumigation alone.After treatment for 2 weeks,the clinical efficacy of both groups was evaluated,and the changes in the scores of the Western Ontario and McMaster Universities Arthritis Index (WOMAC)were observed.Moreover,the contents of visfatin and chemerin in jointfluid were examined.Results (1) The total effective rate of the treatment group was 96.7％ and that of the control group was 83.3％,the difference being significant (P ＜ 0.01).(2) After treatment,WOMAC scores of both groups were obviously decreased(P ＜ 0.01 compared with those before treatment),and the decrease in the treatment group was superior to that in the control group (P ＜ 0.01).(3) The contents of visfatin and chemerint in joint synovial fluid of both groups were decreased (P ＜ 0.01 compared with those before treatment),and the decrease in the treatment group was superior to that in the control group(P ＜ 0.05).Conclusion The comprehensive Chinese medicine therapy of Chinese herbal medicine fumigation,massage and quadriceps exercise is effective for the treatment of KOA,and can decrease the contents of visfatin and chemerin in joint fluid of KOA patients,which may be one of its therapeutic mechanisms.

Identification of herbal tea ingredient Plumeria rubra and its adulterants using DNA barcoding / 中国中药杂志

ITS2 sequence was used as a barcode to identify herbal tea ingredient Plumeria rubra and its adulterants. Genomic DNAs from forty eight samples were extracted, the ITS2 sequences were amplified and sequenced bi-direstionlly, and then assembled and obtained using CodonCode Aligner. The sequences were aligned using ClustalW, the genetic distances were computed by kimura 2-parameter (K2P) model and the Neighbor-joining (NJ) phylogenetic trees were constructed using MEGA5.0. Results showed that the length of ITS2 sequence of P. rubra were 244 bp. The intra-specific genetic distances (0-0. 016 6) were much smaller than inter-specific ones between P. rubra and its adulterants(0.320 8-0.650 4). The NJ tree indicated that P. rubra and its adulterants could be distinguished clearly. Therefore, Using ITS2 barcode can accurately andeffectively distinguish herbal tea ingredient P. rubra from its adulterants, which providesa new molecular method to identify P. rubra and ensure its safety in use.

Factor of Prunella vulgaris yield components and morphological characteristics of relationship in different populations / 中国中药杂志

Prunella vulgaris is a perennial herb whose dry spike is used for medicine in China. It is also a very important traditional medicine. Current study was conducted to the biomass components factors of P. vulgaris in the different ecological environments for the development and protection. The results showed that the contribution factor of the production in different environments was significant differences. In favorable environment, the length of leaf, numbers of leaves, height, reproductive branch number of individuals gave the greatest contribution. In transitional habitats, the diameter of stem and spike, the length of leaf contributed the most. While in the undergrowth and dry hillside, the length of root, the width of leaf, the length of spike were the main factors for biomass.

Nardosinone protects H9c2 cardiac cells from angiotensin II-induced hypertrophy / 华中科技大学学报（医学）（英德文版）

Pathological cardiac hypertrophy induced by angiotensin II (AngII) can subsequently give rise to heart failure, a leading cause of mortality. Nardosinone is a pharmacologically active compound extracted from the roots of Nardostachys chinensis, a well-known traditional Chinese medicine. In order to investigate the effects of nardosinone on AngII-induced cardiac cell hypertrophy and the related mechanisms, the myoblast cell line H9c2, derived from embryonic rat heart, was treated with nardosinone (25, 50, 100, and 200 μmol/L) or AngII (1 μmol/L). Then cell surface area and mRNA expression of classical markers of hypertrophy were detected. The related protein levels in PI3K/Akt/mTOR and MEK/ERK signaling pathways were examined by Western blotting. It was found that pretreatment with nardosinone could significantly inhibit the enlargement of cell surface area induced by AngII. The mRNA expression of ANP, BNP and β-MHC was obviously elevated in AngII-treated H9c2 cells, which could be effectively blocked by nardosinone at the concentration of 100 μmol/L. Further study revealed that the protective effects of nardosinone might be mediated by repressing the phosphorylation of related proteins in PI3K/Akt and MEK/ERK signaling pathways. It was suggested that the inhibitory effect of nardosinone on Ang II-induced hypertrophy in H9c2 cells might be mediated by targeting PI3K/Akt and MEK/ERK signaling pathways.

Nardosinone protects H9c2 cardiac cells from angiotensin II-induced hypertrophy / 华中科技大学学报（医学）（英德文版）

Pathological cardiac hypertrophy induced by angiotensin II (AngII) can subsequently give rise to heart failure, a leading cause of mortality. Nardosinone is a pharmacologically active compound extracted from the roots of Nardostachys chinensis, a well-known traditional Chinese medicine. In order to investigate the effects of nardosinone on AngII-induced cardiac cell hypertrophy and the related mechanisms, the myoblast cell line H9c2, derived from embryonic rat heart, was treated with nardosinone (25, 50, 100, and 200 μmol/L) or AngII (1 μmol/L). Then cell surface area and mRNA expression of classical markers of hypertrophy were detected. The related protein levels in PI3K/Akt/mTOR and MEK/ERK signaling pathways were examined by Western blotting. It was found that pretreatment with nardosinone could significantly inhibit the enlargement of cell surface area induced by AngII. The mRNA expression of ANP, BNP and β-MHC was obviously elevated in AngII-treated H9c2 cells, which could be effectively blocked by nardosinone at the concentration of 100 μmol/L. Further study revealed that the protective effects of nardosinone might be mediated by repressing the phosphorylation of related proteins in PI3K/Akt and MEK/ERK signaling pathways. It was suggested that the inhibitory effect of nardosinone on Ang II-induced hypertrophy in H9c2 cells might be mediated by targeting PI3K/Akt and MEK/ERK signaling pathways.

Study of HPLC-DAD fingerprint on complex traditional Chinese medicine proprietary preparation-Baoji pills / 中国中药杂志

<p><b>OBJECTIVE</b>Based on 'Back-tracking' method, identification and quality evaluation of complex traditional Chinese medicine (TCM) preparation of Baoji pills (BJP) were carried out by HPLC fingerprint analysis.</p><p><b>METHOD</b>HPLC-DAD fingerprint of BJP was conducted with Zorbax SB-C18 column and non-linear elution with the mobile phase consisted of acetonitrile-0.5% glacial acetic acid at column temperature 30 degrees C and detective wavelengths of 250 nm and 283 nm. From the established chromatographic pattern of BJP, track backward to the corresponding crude herbal drugs in the formula, attribution ofmost peaks in the BJP fingerprint can be disclosed.</p><p><b>RESULT</b>The BJP HPLC fingerprint consisted of 44 peaks among which 35 peaks were assigned by parallel comparison with the fingerprint of the 10 corresponding crude drugs in the formula such as pueraria, pummelo peel, and magnolia bark, etc. and 22 peaks we reidentified by comparison with the chemical reference substances.</p><p><b>CONCLUSION</b>The established HPLC fingerprint represents the whole character of BJP, which enhanced the specialty for control and assessment of the product quality. It exemplified much more effective for quality control than selecting any marker for qualitative or quantitative testing target. And the Back-tracking' experimental method extended the study mentality for complex formula TCM products chromatographic fingerprinting analysis.</p>

Effect of angong niuhuang pill as an adjuvant treatment on moderate or severe neonatal hypoxic-ischemic Encephalopathy / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To evaluate the clinical efficacy and safety of angong niuhuang pill (ANP) as an adjuvant treatment on moderate or severe neonatal hypoxic-ischemic encephalopathy (NHIE).</p><p><b>METHODS</b>Thirty-nine neonates with NHIE in the control group were treated with conventional treatment, and 58 in the treated group were administered orally ANP additionally, and relative indexes were observed.</p><p><b>RESULTS</b>The improvement of aspects such as recovery of consciousness, muscular tension, and primitive reflex and disappearance of convulsion, in the treated group was better than that in the control group (P < 0.01).</p><p><b>CONCLUSION</b>ANP as an adjuvant treatment has a definite effect on NHIE, it can promote the recovery of patients, decrease the occurrence of sequelae and with high safety, therefore, is a drug feasible for clinical application.</p>

Changes of dehydroandrographolide's contents of andrographis tablet in the process of production / 中国中药杂志

<p><b>OBJECTIVE</b>To recognize changes in the contents of ingredients of Andrographis Tablet in the process of production.</p><p><b>METHOD</b>Adopting TLCS, TLC, HPLC to detect effective contents of ingredients which are produced in every stage of process of Andrographis Table's production.</p><p><b>RESULT</b>Handling with the fresh Herba Andrographis according to current pharmacopeoia's technology, it showed that only dehyandrographolide can be detected. It indicated that the main factor that leads to chemical change is the heating process in the process of production.</p><p><b>CONCLUSION</b>Avoiding heating treatment or reducing heating treatment time is the main factor to protect the effective ingredients.</p>