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Eur J Pharmacol ; 895: 173880, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33476654

RESUMO

Classic glucocorticoids have been prescribed for various inflammatory diseases, such as rheumatoid arthritis, due to their outstanding anti-inflammatory effects. However, glucocorticoids cause numerous unwanted side effects, including osteoporosis and diabetes. Hence, selective glucocorticoid receptor modulators (SGRMs), which retain anti-inflammatory effects with minimized side effects, are among the most anticipated drugs in the clinical field. The assumption is that there are two major mechanisms of action via glucocorticoid receptors, transrepression (TR) and transactivation (TA). In general, anti-inflammatory effects of glucocorticoids are largely due to TR, while the side effects associated with glucocorticoids are mostly mediated through TA. We previously reported that JTP-117968, a novel SGRM, maintained partial TR activity while remarkably reducing the TA activity. In this study, we investigated the anti-inflammatory effect of JTP-117968 on a lipopolysaccharide (LPS) challenge model and collagen-induced arthritis (CIA) model in mice. Meanwhile, we tested the effect of JTP-117968 on the bone mineral density (BMD) in mouse femur to evaluate the side effect. Based on the evaluation, JTP-117968 reduced the plasma levels of tumor necrosis factor α induced by LPS challenge in mice significantly. Remarkably, CIA development was suppressed by JTP-117968 comparably with prednisolone and PF-802, an active form of fosdagrocorat that has been developed clinically as an orally available SGRM. Strikingly, the side effect of JTP-117968 on mouse femoral BMD was much lower than those of PF-802 and prednisolone. Therefore, JTP-117968 has attractive potential as a new therapeutic option against inflammatory diseases with minimized side effects compared to classic glucocorticoids.


Assuntos
Aminopiridinas/farmacologia , Anti-Inflamatórios/farmacologia , Artrite Experimental/prevenção & controle , Densidade Óssea/efeitos dos fármacos , Glucocorticoides/farmacologia , Articulações/efeitos dos fármacos , Fenantrolinas/farmacologia , Receptores de Glucocorticoides/agonistas , Aminopiridinas/toxicidade , Animais , Anti-Inflamatórios/toxicidade , Artrite Experimental/metabolismo , Artrite Experimental/patologia , Feminino , Glucocorticoides/toxicidade , Humanos , Mediadores da Inflamação/sangue , Peptídeos e Proteínas de Sinalização Intercelular/genética , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Articulações/metabolismo , Articulações/patologia , Masculino , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos DBA , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Fenantrolinas/toxicidade , Receptores de Glucocorticoides/metabolismo , Fator de Necrose Tumoral alfa/sangue
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