RESUMO
The quercetin-molecularly imprinted polymers with specific affinity and selectivity were prepared by using methacrylic acid (MAA), acrylamide (AM), N,N-(diethylamino) ethyl methacrylate and 2-vinylpyridine as functional monomers, respectively. The adsorption properties for template were studied by equilibrium binding experiments. The results showed 2-VP and DEAEM based on ionic interaction with quercetin possessed better imprinting effects. Using the quercetin-imprinted polymers as thin layer chromatographic stationary could successfully separate the template from the other structurally related compounds. In addition, the influence on adsorption effect about the particle size and repeating times of MIP were investigated.
Assuntos
Cromatografia em Camada Fina/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Polímeros/química , Quercetina/química , Adsorção , Cromatografia em Camada Fina/instrumentação , Reagentes de Ligações Cruzadas/química , Medicamentos de Ervas Chinesas/química , Impressão Molecular , Polímeros/isolamento & purificação , Quercetina/isolamento & purificação , Reprodutibilidade dos Testes , Tecnologia Farmacêutica/instrumentação , Tecnologia Farmacêutica/métodosRESUMO
Six chemical components were got from the alcohol extract of Cirsium segestum, their structures were identified by UV, IR, NMR, MS and EA. They were 5,7-dihydroxyflavone, baicalin, oleanolic acid, cholesterol, methyl ursolate and rutin. The first to the fifth of which were got from Cirsium segestum for the first time.