Effect of tea polyphenols on the adhesion of highly metastatic human lung carcinoma cell lines to endothelial cells in vitro.

AIM: Tea polyphenols are known to play roles in critical steps of human lung carcinoma cell metastasis. For understanding the mechanisms whereby they inhibit tumor metastasis, the present study was conducted to investigate their effects on the adhesion of highly metastatic lung carcinoma cell lines (PG cells) to endothelial cells (EC cells) and adhesion molecule expression in vitro. METHODS: The expression of CD44 or CD54 in the PG cells was detected by flow cytometry and adhesion of PG cells to EC cells was assessed by confocal microscopy double fluorescence staining. RESULTS: The results showed that tea polyphenols: (1) inhibited the expression of CD44 and CD54, two important adhesion molecules in the PG cells in a dose-dependent manner; (2) significantly blocked the adhesion of PG cells to EC cells not only in a state of rest but also when active; and (3) influenced CD44 and CD54 expression during the adhesion process of PG cells to EC cells. CONCLUSION: The data indicated that the blocking role of tea polyphenols in the adhesion of PG cells to EC cells is related to CD44 and CD54. The mechanism of tea polyphenol prevention of human lung carcinoma metastasis might be through inhibiting adhesion molecule expression to block cancer cell adhesion.

[Simulation study of antithrombotic active fraction of Naodesheng by support vector machine].

OBJECTIVE: To screen the antithrombotic active fraction of Naodesheng. METHODS: The collagen and adrenaline-induced thrombosis by tail intravenous injection in mice was used. Based on orthogonal design, the support vector machine model was established for pharmacodynamics prediction of TCM prescriptions. RESULTS: The model established in this study could predict the drug actions of different combinations. CONCLUSION: The pharmacodynamic actions of several formulas are superior to that of the original formula and CD has the best effect.

A new alkaloid from the stem of Sparganium stoloniferum Buch.-Ham.

A new alkaloid (1), together with five known compounds (2-6), has been isolated from the stem of Sparganium stoloniferum Buch.-Ham. The structure of the new compound was elucidated as 3-isobutyl-tetrahydro-imidazo[1,2-a]pyridine-2,5-dione on the basis of physical and chemical evidence and spectral analysis. Compound 6 was obtained for the first time from the Sparganium genus.

[Survey of studies on the electromagnetic characteristics of acupoints and meridians].

The present paper reviewes the literatures on the electromagnetic characteristic of meridians at home and abroad in the last twenty years. It generally summarize the studies on the electromagnetic characteristic of meridians from the aspects of the imaging of meridians, the electromagnetic model of meridians, and the physiological and pathological observations on the electro changes at acupoints. The results indicate that acupoints apparently have electro characteristics which are associated with the change of the health condition, time, environment, etc. By means of electromagnetic characteristic, the image of meridians can be made and the phenomena of meridians can be clarified, the essence of meridians can be explained in a certain degree. It still demands further research on this characteristic of meridians.

[Development of measurement and control technologies for hyperthermia treatments of tumors with AC magnetic field].

Hyperthermia therapy with AC magnetic field heating magnetic nanoparticles is a new kind of treatment method. The paper reviews the research progresses about AC magnetic heating setups for hyperthermia therapy measurements of magnetic field temperature control, and so on.